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The map label for this gene is yeaT [H]

Identifier: 238895682

GI number: 238895682

Start: 3607978

End: 3608877

Strand: Reverse

Name: yeaT [H]

Synonym: KP1_3779

Alternate gene names: 238895682

Gene position: 3608877-3607978 (Counterclockwise)

Preceding gene: 238895685

Following gene: 238895679

Centisome position: 68.76

GC content: 61.89

Gene sequence:

>900_bases
ATGTTCAGGCTGGAAGATCTGACCTTATTTGTCCGCGCCGCGGCGCTGGGCAGTTTTAGCGACGCGGCGCGCGAGGCCGG
GCAGCAGCCGGCGCAGGTCAGCGCGGCGATTAAGCGTCTGGAGACGATCCTCAATATTCGCCTGTTTGCCCGTTCGACCC
GCAGCCTGCGCCTGACGCCGGAGGGGGAGACCTGGCTGCCCTACGCCACCCAGATGCTCGATACTCTCGAGGCCGGTCTG
CAGAAGATCCAAACCCCGGACGATGAAGTTCGCGGCATGTTGCAAATTGCCGTGCCGTCGGACCTCGGGCGTAATCTGCT
CCTGACCCTGTTCCGCGATTTCCGCCAACGCCACCCGGCGCTGCGCCTGCGGCTGCTCTTTTCCGATCAGCTGACCGACG
TGTTTAAAGATCCGGTGGACGTTGCGTTTCGCTATGGCAATAACGACGATGCCTCGTTTATCTCGCTGCCGGTGGCGCCG
GAAAATCGTCGGGTGCTGGTGGCCTCGCCTGAGTGGATCGCCCGCCATGGCGAACCGCAGACCCTCGAGGAACTCAGCCA
GCACAATGCGTTGATCTACATTTTGCGCGGCCGTCCGTTCGACCGCTGGTCGCTGAGCCTCGACGGCGTGGTTCAGCAGC
AGAAGGTTTCCGGCACGGTGATGAGCGACGACGCCGAAGTGATCCGTCGGCTGGCGATCGCCGGGGAGGGCATTGCCTAT
AAATCGATGCTCGACGTCAGCGACGATCTGCGGGCGGGGCGCCTGCGGCGCCTGCTGCCGCGTTATCAGGGGGATGTAGT
GCCGTTGAATCTGATTTGTCCGCACCGCAAACAGCTCTCTGCCGCGGTGCGTTTGTTATATGAAGAGGTGAAATCACATT
GTGAAGGGCTTAATGCCTGA

Upstream 100 bases:

>100_bases
TAAAAAGTGTAAGCAAAACGCGGCGCAAGTCTTGTGCCGCTTGCGTTGCAGCGACGGTGATGCGTATTTTGGGAGCAATC
TGAAGCAGGGGAGAATCGGC

Downstream 100 bases:

>100_bases
CTAAGGTCGCGAAGTAATACACCAGCGGGACGGCCAATACCCACAGGCCAATCGGGATCTCCCGCCACTTACCGGCGATA
GCCTTGATCACAATGTAAAA

Product: putative LysR-family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MFRLEDLTLFVRAAALGSFSDAAREAGQQPAQVSAAIKRLETILNIRLFARSTRSLRLTPEGETWLPYATQMLDTLEAGL
QKIQTPDDEVRGMLQIAVPSDLGRNLLLTLFRDFRQRHPALRLRLLFSDQLTDVFKDPVDVAFRYGNNDDASFISLPVAP
ENRRVLVASPEWIARHGEPQTLEELSQHNALIYILRGRPFDRWSLSLDGVVQQQKVSGTVMSDDAEVIRRLAIAGEGIAY
KSMLDVSDDLRAGRLRRLLPRYQGDVVPLNLICPHRKQLSAAVRLLYEEVKSHCEGLNA

Sequences:

>Translated_299_residues
MFRLEDLTLFVRAAALGSFSDAAREAGQQPAQVSAAIKRLETILNIRLFARSTRSLRLTPEGETWLPYATQMLDTLEAGL
QKIQTPDDEVRGMLQIAVPSDLGRNLLLTLFRDFRQRHPALRLRLLFSDQLTDVFKDPVDVAFRYGNNDDASFISLPVAP
ENRRVLVASPEWIARHGEPQTLEELSQHNALIYILRGRPFDRWSLSLDGVVQQQKVSGTVMSDDAEVIRRLAIAGEGIAY
KSMLDVSDDLRAGRLRRLLPRYQGDVVPLNLICPHRKQLSAAVRLLYEEVKSHCEGLNA
>Mature_299_residues
MFRLEDLTLFVRAAALGSFSDAAREAGQQPAQVSAAIKRLETILNIRLFARSTRSLRLTPEGETWLPYATQMLDTLEAGL
QKIQTPDDEVRGMLQIAVPSDLGRNLLLTLFRDFRQRHPALRLRLLFSDQLTDVFKDPVDVAFRYGNNDDASFISLPVAP
ENRRVLVASPEWIARHGEPQTLEELSQHNALIYILRGRPFDRWSLSLDGVVQQQKVSGTVMSDDAEVIRRLAIAGEGIAY
KSMLDVSDDLRAGRLRRLLPRYQGDVVPLNLICPHRKQLSAAVRLLYEEVKSHCEGLNA

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=32.1917808219178, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786401, Length=256, Percent_Identity=32.421875, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=31.7241379310345, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1789440, Length=293, Percent_Identity=28.3276450511945, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787589, Length=289, Percent_Identity=26.643598615917, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=27.3648648648649, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1787128, Length=297, Percent_Identity=26.5993265993266, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI157672245, Length=173, Percent_Identity=31.2138728323699, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789173, Length=256, Percent_Identity=29.296875, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786448, Length=190, Percent_Identity=31.5789473684211, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082132, Length=294, Percent_Identity=27.891156462585, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786508, Length=98, Percent_Identity=38.7755102040816, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788706, Length=192, Percent_Identity=28.6458333333333, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=124, Percent_Identity=26.6129032258064, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33697; Mature: 33697

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRLEDLTLFVRAAALGSFSDAAREAGQQPAQVSAAIKRLETILNIRLFARSTRSLRLTP
CCCCHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEECCCEEEECC
EGETWLPYATQMLDTLEAGLQKIQTPDDEVRGMLQIAVPSDLGRNLLLTLFRDFRQRHPA
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCHH
LRLRLLFSDQLTDVFKDPVDVAFRYGNNDDASFISLPVAPENRRVLVASPEWIARHGEPQ
HEEHHHHHHHHHHHHHCCHHEEEEECCCCCCCEEEEECCCCCCEEEEECCHHHHHCCCCH
TLEELSQHNALIYILRGRPFDRWSLSLDGVVQQQKVSGTVMSDDAEVIRRLAIAGEGIAY
HHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEECCHHHHHHHHHHCCCCCHH
KSMLDVSDDLRAGRLRRLLPRYQGDVVPLNLICPHRKQLSAAVRLLYEEVKSHCEGLNA
HHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFRLEDLTLFVRAAALGSFSDAAREAGQQPAQVSAAIKRLETILNIRLFARSTRSLRLTP
CCCCHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEECCCEEEECC
EGETWLPYATQMLDTLEAGLQKIQTPDDEVRGMLQIAVPSDLGRNLLLTLFRDFRQRHPA
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCEEEEEECCCCCCHHHHHHHHHHHHHHCHH
LRLRLLFSDQLTDVFKDPVDVAFRYGNNDDASFISLPVAPENRRVLVASPEWIARHGEPQ
HEEHHHHHHHHHHHHHCCHHEEEEECCCCCCCEEEEECCCCCCEEEEECCHHHHHCCCCH
TLEELSQHNALIYILRGRPFDRWSLSLDGVVQQQKVSGTVMSDDAEVIRRLAIAGEGIAY
HHHHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCEECCHHHHHHHHHHCCCCCHH
KSMLDVSDDLRAGRLRRLLPRYQGDVVPLNLICPHRKQLSAAVRLLYEEVKSHCEGLNA
HHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]