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The map label for this gene is alkA [H]

Identifier: 238895639

GI number: 238895639

Start: 3552859

End: 3553707

Strand: Reverse

Name: alkA [H]

Synonym: KP1_3731

Alternate gene names: 238895639

Gene position: 3553707-3552859 (Counterclockwise)

Preceding gene: 238895645

Following gene: 238895638

Centisome position: 67.71

GC content: 68.55

Gene sequence:

>849_bases
ATGGTGCTGTTACCCTGGACCCCGCCCTATGACTGGGCGTGGATGGTTGGTTTTCTGCAGGCGCGCGCGGTTGCCGGCGT
CGAGCGTTTTCACGATGGGGGCTACAGCCGCAGCTTTGGCGTGGAAGGCCACCGCGGGCTGATTCATCTGGCGCCGGACG
AAGAGGCGCAGGGCCTGCGGGTGACCCTTTCCCCGGGACTGCAGCCGGTGGCGGAGATCTGCTATGCGCGAATTGGCCAG
CTGTTTGATTTAGCCTGCGACCCGCGGCAGGTCGCTCGCACGCTGGGCGACCTGGCGCAGGCGCGGCCGGGGCTGCGCCT
GCCGGGGGCGCTGGATGCCTTCGAGCAGGCGGTGCGCGCGGTGCTGGGCCAGCTGGTGAGCGTGGCGATGGCCGCCCGGC
TCACCGCCAAAGTGGCGGCTGGCTGGGGCGAACCGCTGGCGGAGGCGCCTGGATATGTCTTGTTCCCGACGCCAGAGGCG
CTGTCCCGGGCCGATCCGCAGGCGCTGAAGGCGCTGGGGATGCCGCTGCGACGCGCGGAGGCGCTGATCCACCTCGCCCG
GGCGGCGCTCAGCGGCGAGCTGCCCCTGACGGCGCCGGCGGATATTGACGCCGGGCTGCGTCAACTGCAGACCCTGCCTG
GGATTGGCCGCTGGACGGCAAACTACTTTGCCCTGCGCGGCTGGCAGGCGAAAGATATCTTTTTGCCAGATGATTACCTG
ATTAAACAGCGCTTTCCGGGAATGACCCCGGCCGCCATCGCCCGCTATGCCCGCCGCTGGCAGCCGATGCGATCCTACGC
GCTGTTGCATATCTGGTATACCGATGACTGGGCGCCGGCGGCGGAATAG

Upstream 100 bases:

>100_bases
GACTTTAGCGGATCGTCGCCATGCCGACAACCGGAATTTAAAAGCAAGGCGCTGATGGTCAAGGGCGACTATCATTGAAA
CGCTATATAAAGGAGAGCTT

Downstream 100 bases:

>100_bases
CCGCCCGGGGCGCATACCACTGGTATCGGGACGCTGAAATCCCTATAATTGCCGCGTTTGGCGGTCCGCCGCCACCCTCC
ATCTCTATCCAGGTTAATCA

Product: 3-methyl-adenine DNA glycosylase II

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase II, inducible; TAG II; DNA-3-methyladenine glycosidase II; DNA-3-methyladenine glycosylase II [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MVLLPWTPPYDWAWMVGFLQARAVAGVERFHDGGYSRSFGVEGHRGLIHLAPDEEAQGLRVTLSPGLQPVAEICYARIGQ
LFDLACDPRQVARTLGDLAQARPGLRLPGALDAFEQAVRAVLGQLVSVAMAARLTAKVAAGWGEPLAEAPGYVLFPTPEA
LSRADPQALKALGMPLRRAEALIHLARAALSGELPLTAPADIDAGLRQLQTLPGIGRWTANYFALRGWQAKDIFLPDDYL
IKQRFPGMTPAAIARYARRWQPMRSYALLHIWYTDDWAPAAE

Sequences:

>Translated_282_residues
MVLLPWTPPYDWAWMVGFLQARAVAGVERFHDGGYSRSFGVEGHRGLIHLAPDEEAQGLRVTLSPGLQPVAEICYARIGQ
LFDLACDPRQVARTLGDLAQARPGLRLPGALDAFEQAVRAVLGQLVSVAMAARLTAKVAAGWGEPLAEAPGYVLFPTPEA
LSRADPQALKALGMPLRRAEALIHLARAALSGELPLTAPADIDAGLRQLQTLPGIGRWTANYFALRGWQAKDIFLPDDYL
IKQRFPGMTPAAIARYARRWQPMRSYALLHIWYTDDWAPAAE
>Mature_282_residues
MVLLPWTPPYDWAWMVGFLQARAVAGVERFHDGGYSRSFGVEGHRGLIHLAPDEEAQGLRVTLSPGLQPVAEICYARIGQ
LFDLACDPRQVARTLGDLAQARPGLRLPGALDAFEQAVRAVLGQLVSVAMAARLTAKVAAGWGEPLAEAPGYVLFPTPEA
LSRADPQALKALGMPLRRAEALIHLARAALSGELPLTAPADIDAGLRQLQTLPGIGRWTANYFALRGWQAKDIFLPDDYL
IKQRFPGMTPAAIARYARRWQPMRSYALLHIWYTDDWAPAAE

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, 3-methylguanine, 7-methylguanine, O2- methylthymine, and O2-methylcytosine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkylbase DNA glycosidase alkA family [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=279, Percent_Identity=65.5913978494624, Blast_Score=375, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010316
- InterPro:   IPR000035
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =3.2.2.21 [H]

Molecular weight: Translated: 30882; Mature: 30882

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00516 ALKYLBASE_DNA_GLYCOS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLLPWTPPYDWAWMVGFLQARAVAGVERFHDGGYSRSFGVEGHRGLIHLAPDEEAQGLR
CEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCEE
VTLSPGLQPVAEICYARIGQLFDLACDPRQVARTLGDLAQARPGLRLPGALDAFEQAVRA
EEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
VLGQLVSVAMAARLTAKVAAGWGEPLAEAPGYVLFPTPEALSRADPQALKALGMPLRRAE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEECCHHHHHCCCHHHHHHHCCCHHHHH
ALIHLARAALSGELPLTAPADIDAGLRQLQTLPGIGRWTANYFALRGWQAKDIFLPDDYL
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHEECCCCCEEECCCHHH
IKQRFPGMTPAAIARYARRWQPMRSYALLHIWYTDDWAPAAE
HHHHCCCCCHHHHHHHHHHCCHHHCEEEEEEEEECCCCCCCC
>Mature Secondary Structure
MVLLPWTPPYDWAWMVGFLQARAVAGVERFHDGGYSRSFGVEGHRGLIHLAPDEEAQGLR
CEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCEE
VTLSPGLQPVAEICYARIGQLFDLACDPRQVARTLGDLAQARPGLRLPGALDAFEQAVRA
EEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
VLGQLVSVAMAARLTAKVAAGWGEPLAEAPGYVLFPTPEALSRADPQALKALGMPLRRAE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEECCHHHHHCCCHHHHHHHCCCHHHHH
ALIHLARAALSGELPLTAPADIDAGLRQLQTLPGIGRWTANYFALRGWQAKDIFLPDDYL
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHEECCCCCEEECCCHHH
IKQRFPGMTPAAIARYARRWQPMRSYALLHIWYTDDWAPAAE
HHHHCCCCCHHHHHHHHHHCCHHHCEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6094528; 9097040; 9278503; 6389535; 3529081; 8706135; 8706136 [H]