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The map label for this gene is cobS [H]

Identifier: 238895585

GI number: 238895585

Start: 3487590

End: 3488330

Strand: Reverse

Name: cobS [H]

Synonym: KP1_3668

Alternate gene names: 238895585

Gene position: 3488330-3487590 (Counterclockwise)

Preceding gene: 238895586

Following gene: 238895584

Centisome position: 66.46

GC content: 65.59

Gene sequence:

>741_bases
GTGATTAAGTCATTCTTTGCTGCACTCTCGTTTATCAGCCGCCTGCCGGTCCCGGCGCGGTTATCTCAGGGCCTGGAGAT
CGAACAGTACCAGCGCAGTATCGTCACCTTCCCGCTGGTCGGTTTACTGCTCGGCGCCATCGCCGGCGCGGTGGCGCTCC
TCCTGCAGCCGTGGTGCGGCGTCCCGCTGGCGGCGCTGTTCGGCGTGCTGGCGCTGGCGCTGCTCACCGGCGGATTCCAT
CTTGACGGGCTGGCTGATACCTGCGACGGCATTTTCTCCGCCCGCACCCGCGATCGGATGCTGGAGATCATGCGCGACAG
CCGGCTGGGCACTCACGGCGGGCTGGCGCTGATCTTCGTGCTGGTGGCGAAAGTGCTGGTGGTTGGCGAGCTGCTGCTGC
GTGATATCCACCCCATCGCAGCCCTGGCGGCGGCCTGCGCCGTCGGTCGCGGGATGGCGGTGCTGCTGATGTATCGTCAT
CGCTATGCGCGGGAAAAGGGGCTGGGCAATCTGTTTATCGGCAAAATTAGCCTGCAGCAGACGCTGGTGACGATGGCGAT
GGCGATCGCGCTGGCCACCGCCCTGCTGGGGCTGCAGGGGCTGCGCGCCGCGCTGATCACCCTGGTGCTGATCTGGGGTC
TGGGATGGGCGCTGAAGCGAACCCTCGGCGGGCAGACCGGGGATACCCTCGGGGCGGCGATTGAGCTGGGCGAATTACTC
TTTTTACTGGCGCTGCTGTAA

Upstream 100 bases:

>100_bases
CTCACCTCTCCGACGATCGATTCGCCAGAAGACAAACTCAGGTGCTGACCGGGCGCGACAGAACTCTTCCGCTGCTTTCG
CCGACAGGAGTCAACCTCAC

Downstream 100 bases:

>100_bases
GGCCTGACGCCAACGGTGGGGAACAGAATGGAAACACTTTCGGCGTTACTGGCTGCCATTCCGCAGCCCGATGTGGCGGC
CATGGCGCGCGCTCAGCAGC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MIKSFFAALSFISRLPVPARLSQGLEIEQYQRSIVTFPLVGLLLGAIAGAVALLLQPWCGVPLAALFGVLALALLTGGFH
LDGLADTCDGIFSARTRDRMLEIMRDSRLGTHGGLALIFVLVAKVLVVGELLLRDIHPIAALAAACAVGRGMAVLLMYRH
RYAREKGLGNLFIGKISLQQTLVTMAMAIALATALLGLQGLRAALITLVLIWGLGWALKRTLGGQTGDTLGAAIELGELL
FLLALL

Sequences:

>Translated_246_residues
MIKSFFAALSFISRLPVPARLSQGLEIEQYQRSIVTFPLVGLLLGAIAGAVALLLQPWCGVPLAALFGVLALALLTGGFH
LDGLADTCDGIFSARTRDRMLEIMRDSRLGTHGGLALIFVLVAKVLVVGELLLRDIHPIAALAAACAVGRGMAVLLMYRH
RYAREKGLGNLFIGKISLQQTLVTMAMAIALATALLGLQGLRAALITLVLIWGLGWALKRTLGGQTGDTLGAAIELGELL
FLLALL
>Mature_246_residues
MIKSFFAALSFISRLPVPARLSQGLEIEQYQRSIVTFPLVGLLLGAIAGAVALLLQPWCGVPLAALFGVLALALLTGGFH
LDGLADTCDGIFSARTRDRMLEIMRDSRLGTHGGLALIFVLVAKVLVVGELLLRDIHPIAALAAACAVGRGMAVLLMYRH
RYAREKGLGNLFIGKISLQQTLVTMAMAIALATALLGLQGLRAALITLVLIWGLGWALKRTLGGQTGDTLGAAIELGELL
FLLALL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=247, Percent_Identity=71.2550607287449, Blast_Score=336, Evalue=7e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26062; Mature: 26062

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSFFAALSFISRLPVPARLSQGLEIEQYQRSIVTFPLVGLLLGAIAGAVALLLQPWCG
CHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VPLAALFGVLALALLTGGFHLDGLADTCDGIFSARTRDRMLEIMRDSRLGTHGGLALIFV
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LVAKVLVVGELLLRDIHPIAALAAACAVGRGMAVLLMYRHRYAREKGLGNLFIGKISLQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
TLVTMAMAIALATALLGLQGLRAALITLVLIWGLGWALKRTLGGQTGDTLGAAIELGELL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
FLLALL
HHHHHC
>Mature Secondary Structure
MIKSFFAALSFISRLPVPARLSQGLEIEQYQRSIVTFPLVGLLLGAIAGAVALLLQPWCG
CHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VPLAALFGVLALALLTGGFHLDGLADTCDGIFSARTRDRMLEIMRDSRLGTHGGLALIFV
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LVAKVLVVGELLLRDIHPIAALAAACAVGRGMAVLLMYRHRYAREKGLGNLFIGKISLQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
TLVTMAMAIALATALLGLQGLRAALITLVLIWGLGWALKRTLGGQTGDTLGAAIELGELL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
FLLALL
HHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA