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The map label for this gene is dcm [H]

Identifier: 238895488

GI number: 238895488

Start: 3373382

End: 3374815

Strand: Reverse

Name: dcm [H]

Synonym: KP1_3560

Alternate gene names: 238895488

Gene position: 3374815-3373382 (Counterclockwise)

Preceding gene: 238895489

Following gene: 238895487

Centisome position: 64.3

GC content: 59.41

Gene sequence:

>1434_bases
ATGCCGCAAAAGTTAAGCAAGGAACACAGGTCGTTGTCGTTGGCTGAACAGGCCGGGGAAGACGCTGAGGCGCTGCTGCG
TCAGCTGATGACGATCTACGATGTAAAAACGCTGGTTGCCGAACTGGTGAGCGTCGGCGAACAGCACTGGAGCGCGGCGA
TCCTCAAGCGCGTGGTGGCGCTTGGCCGCGTGGCGGAGCGTTTGCGCCCGCAGGAAGTTGCTCATCTGGCGACGCTCCTG
CCTTCGCCGCCGGCCCATCATCCGCACTATGGGTTCCGTTTTATCGATCTGTTTGCCGGTATTGGCGGGATCCGCAGTGG
TTTTGAAGCCATCGGCGGGCAGTGCGTTTTCACCAGCGAGTGGAACAAGCACGCGGTACGCACCTATAAGGCCAACTGGT
ACTGTGATCCGCAGCAGCACCGTTTTAATGAAGATATCCGCGATATTACCCTCAGCCAACGCTCTGACGTTAGCGACGAG
GAGGCGGCGCGGCATATTCGCGAATCTATCCCTCAGCATGATGTGCTGCTGGCTGGCTTCCCCTGCCAGCCTTTCTCGCT
TGCCGGCGTCTCTAAGAAAAATGCGATGGGCCGTGCGCATGGCTTTGCCTGCGAAACCCAGGGAACGCTGTTTTTTGACG
TGGTGAGAATTATCGCGGCTCGTCAGCCAGCGATTTTTGTGCTGGAGAATGTCAAAAACCTCAAAAGCCACGATCAGGGG
CGCACTTTCCGTATCATCATGCAGACGCTGGATGAGCTGGGATATGAAGTAGCTGACGCGGGGCATACCGGGCCGGATGA
CCCGAAGGTGATTGACGGGCGCCATTTCTTGCCTCAACACCGGGAGCGCATCGTGCTGGTGGGTTTCCGTCGCGATCTGC
AGCTGCATGCTGGATTTACGCTGCGCGACATCGCGGCGCAGTACCCGGCGGTGCGGCCAACATTTGGCGAGCTGCTGGAG
CCGACGGTCGATGCGAAATTTATTCTTACGCCGGTGCTGTGGAAATATCTTTATCGTTATGCCCGCAAGCATCAGGCGCG
GGGCAATGGCTTTGGTTATGGCCTGGTTGACCCCGCCAATCCCCACAGCGTGGCCCGGACGCTTTCTGCCCGCTACTATA
AAGATGGTGCCGAAATTCTCGTCGATCGCGGCTGGGACCGGCCGCTCGGTGAGAAGCATTTTGACGATCCGCTGAATCAA
CAGCGGCGGCCTCGCCGGTTGACGCCGCGCGAATGCGCCCGGCTGATGGGCTTTGAATCCCCGCAGGGGGCGCGTTTTCG
CATCCCGGTATCGGATACGCAGGCCTATCGTCAGTTTGGCAACTCGGTGGTGGTTCCGGTCTTCGCCGCGGTGGCGAAAC
TCCTGGCGCCGCGCATCGCTCAGGCGGTGGCGCGCCGTGAAGCGGATGATAATGATGGCGGATGTTCACGATAA

Upstream 100 bases:

>100_bases
GCGCTGGATGAAGCGGTTTTGCGCCGCTTTGCACCGCAGGCGTCAACCGACCGGTGATTTATGGTACTCTCTGCCAAAAT
CGTCCGCGTTGGTTGAGGAT

Downstream 100 bases:

>100_bases
AGCGACGCGCAGTAAAAATATGCGGGCGATCGCCACGCGCGATACGGCGATCGAAAAGCGGATAGCGGCGCTGCTGACCG
GGGCGGGATTCACCTTTGTC

Product: DNA cytosine methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MPQKLSKEHRSLSLAEQAGEDAEALLRQLMTIYDVKTLVAELVSVGEQHWSAAILKRVVALGRVAERLRPQEVAHLATLL
PSPPAHHPHYGFRFIDLFAGIGGIRSGFEAIGGQCVFTSEWNKHAVRTYKANWYCDPQQHRFNEDIRDITLSQRSDVSDE
EAARHIRESIPQHDVLLAGFPCQPFSLAGVSKKNAMGRAHGFACETQGTLFFDVVRIIAARQPAIFVLENVKNLKSHDQG
RTFRIIMQTLDELGYEVADAGHTGPDDPKVIDGRHFLPQHRERIVLVGFRRDLQLHAGFTLRDIAAQYPAVRPTFGELLE
PTVDAKFILTPVLWKYLYRYARKHQARGNGFGYGLVDPANPHSVARTLSARYYKDGAEILVDRGWDRPLGEKHFDDPLNQ
QRRPRRLTPRECARLMGFESPQGARFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLAPRIAQAVARREADDNDGGCSR

Sequences:

>Translated_477_residues
MPQKLSKEHRSLSLAEQAGEDAEALLRQLMTIYDVKTLVAELVSVGEQHWSAAILKRVVALGRVAERLRPQEVAHLATLL
PSPPAHHPHYGFRFIDLFAGIGGIRSGFEAIGGQCVFTSEWNKHAVRTYKANWYCDPQQHRFNEDIRDITLSQRSDVSDE
EAARHIRESIPQHDVLLAGFPCQPFSLAGVSKKNAMGRAHGFACETQGTLFFDVVRIIAARQPAIFVLENVKNLKSHDQG
RTFRIIMQTLDELGYEVADAGHTGPDDPKVIDGRHFLPQHRERIVLVGFRRDLQLHAGFTLRDIAAQYPAVRPTFGELLE
PTVDAKFILTPVLWKYLYRYARKHQARGNGFGYGLVDPANPHSVARTLSARYYKDGAEILVDRGWDRPLGEKHFDDPLNQ
QRRPRRLTPRECARLMGFESPQGARFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLAPRIAQAVARREADDNDGGCSR
>Mature_476_residues
PQKLSKEHRSLSLAEQAGEDAEALLRQLMTIYDVKTLVAELVSVGEQHWSAAILKRVVALGRVAERLRPQEVAHLATLLP
SPPAHHPHYGFRFIDLFAGIGGIRSGFEAIGGQCVFTSEWNKHAVRTYKANWYCDPQQHRFNEDIRDITLSQRSDVSDEE
AARHIRESIPQHDVLLAGFPCQPFSLAGVSKKNAMGRAHGFACETQGTLFFDVVRIIAARQPAIFVLENVKNLKSHDQGR
TFRIIMQTLDELGYEVADAGHTGPDDPKVIDGRHFLPQHRERIVLVGFRRDLQLHAGFTLRDIAAQYPAVRPTFGELLEP
TVDAKFILTPVLWKYLYRYARKHQARGNGFGYGLVDPANPHSVARTLSARYYKDGAEILVDRGWDRPLGEKHFDDPLNQQ
RRPRRLTPRECARLMGFESPQGARFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLAPRIAQAVARREADDNDGGCSR

Specific function: This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=471, Percent_Identity=76.8577494692144, Blast_Score=732, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 53645; Mature: 53514

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQKLSKEHRSLSLAEQAGEDAEALLRQLMTIYDVKTLVAELVSVGEQHWSAAILKRVVA
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGRVAERLRPQEVAHLATLLPSPPAHHPHYGFRFIDLFAGIGGIRSGFEAIGGQCVFTSE
HHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECC
WNKHAVRTYKANWYCDPQQHRFNEDIRDITLSQRSDVSDEEAARHIRESIPQHDVLLAGF
CCCHHHEEEECCEEECHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECC
PCQPFSLAGVSKKNAMGRAHGFACETQGTLFFDVVRIIAARQPAIFVLENVKNLKSHDQG
CCCCCCCCCCCHHHHCHHHCCCEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
RTFRIIMQTLDELGYEVADAGHTGPDDPKVIDGRHFLPQHRERIVLVGFRRDLQLHAGFT
CHHHHHHHHHHHHCCHHHCCCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCEEEECCCH
LRDIAAQYPAVRPTFGELLEPTVDAKFILTPVLWKYLYRYARKHQARGNGFGYGLVDPAN
HHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PHSVARTLSARYYKDGAEILVDRGWDRPLGEKHFDDPLNQQRRPRRLTPRECARLMGFES
HHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCCC
PQGARFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLAPRIAQAVARREADDNDGGCSR
CCCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PQKLSKEHRSLSLAEQAGEDAEALLRQLMTIYDVKTLVAELVSVGEQHWSAAILKRVVA
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGRVAERLRPQEVAHLATLLPSPPAHHPHYGFRFIDLFAGIGGIRSGFEAIGGQCVFTSE
HHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECC
WNKHAVRTYKANWYCDPQQHRFNEDIRDITLSQRSDVSDEEAARHIRESIPQHDVLLAGF
CCCHHHEEEECCEEECHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECC
PCQPFSLAGVSKKNAMGRAHGFACETQGTLFFDVVRIIAARQPAIFVLENVKNLKSHDQG
CCCCCCCCCCCHHHHCHHHCCCEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
RTFRIIMQTLDELGYEVADAGHTGPDDPKVIDGRHFLPQHRERIVLVGFRRDLQLHAGFT
CHHHHHHHHHHHHCCHHHCCCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCEEEECCCH
LRDIAAQYPAVRPTFGELLEPTVDAKFILTPVLWKYLYRYARKHQARGNGFGYGLVDPAN
HHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PHSVARTLSARYYKDGAEILVDRGWDRPLGEKHFDDPLNQQRRPRRLTPRECARLMGFES
HHHHHHHHHHHHHHCCHHHEEECCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHCCCC
PQGARFRIPVSDTQAYRQFGNSVVVPVFAAVAKLLAPRIAQAVARREADDNDGGCSR
CCCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]