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The map label for this gene is nasA [H]

Identifier: 238895279

GI number: 238895279

Start: 3160630

End: 3163335

Strand: Reverse

Name: nasA [H]

Synonym: KP1_3331

Alternate gene names: 238895279

Gene position: 3163335-3160630 (Counterclockwise)

Preceding gene: 238895280

Following gene: 238895278

Centisome position: 60.27

GC content: 67.85

Gene sequence:

>2706_bases
GTGCGACGGCGACGAACAGGCGGTACGCGCCTGGCCGGTGAAAGTGGAAAACGGCAAAGTGTGGGTGGGCAACCAGCAGC
TGCTGGCCCGCGCGGAGGCCTCCTGATGAAGACCATCCGAACGACCTGCCCCTACTGCGGCGTAGGCTGCGGGGTGCTGG
CGAGCGTGGACGACGCGGGGCAGGTGAGCGTGCGCGGCGACGATCAGCACCCGGCGAACCTCGGCCGTCTGTGCGTCAAA
GGGGCCGCCTTAGGGGAGACCACGGGCCTGGCGGGGCGGCTGCTGACGCCGGAGGTCGACGGCCAGCAGGTAGCCTGGCC
TCAGGCGCTGGCGGAAACCGCTGCGCGCCTGAGACAGATCATCGACCAGCACGGACCGCAAGCGGTGGCCTTTTACGCCT
CCGGCCAGCTGTTAACCGAGGATTACTATGCCGCCAATAAGCTGATGAAAGGGTTTATCGGCGCGGCGAACATCGATACC
AATTCACGGCTGTGCATGTCGTCAGCGGTCACTGGCTACAAGCGGGCCTTTGGCGCCGACGTGGTGCCCTGCAGCTACGA
TGATGTGGAAAACAGCGATCTGGTGGTGCTGGTCGGCTCGAACGCCGCCTGGGCGCATCCGGTGCTGTTCCAGCGGCTGG
CGCAGGCGAAGCGGGATAATCCCCGATTGCGGATCGTGGCGATCGACCCCCGACGCACCGCTACCTGCGAGATCGCCGAC
CGCCATCTGGCGCTGGCCCCGGGAAGCGACGGCGGGTTGTTTGCGGGGCTGCTTAACGCCCTGGCTGAGGCAGGAGCCTG
CGTCGATGGCTTTCGCGACGGCCCGCAGGCGCTGGCCGCCGCCCGCGGCTGGGACGTGGCGAGGGTGGCGGCCTTTTGCG
GTCTGCCTGCCGATGAGGTGGCGGGATTCTATCGCGAGTTCATCGCCGCCCCGCGGGCGATCACCCTCTACACCATGGGA
ATCAATCAATCCGCCAGCGGCAGCGACAAATGTAACGCCATCATCAACGTCCATCTGGCCAGCGGGAAGTATGGCCGCCG
CGGCTGCGGCCCCTTTTCGCTGACCGGTCAACCCAACGCCATGGGCGGCCGCGAAGTCGGCGGGCTGGCGACGATGCTGG
CGGCGCACATGGATTTTGTGCCAGACGATCTGCAGCGGCTGGCCCGCTTCTGGGGGACGGAACGGCTGGCGCAGACCCCC
GGGCTGACTGCCGTGGAGCTGTTTGCCGCCATTGGCCGCGGCGAGGTCAAAGCGGTCTGGATCATGGGTACCAACCCGGT
GGTGTCGCTCCCTGACAGCCACGCGGTGAGCCAGGCGCTGGCCGCCTGTCCGCTGGTGATCGTCTCGGATGTCGCCGCCC
AGACCGATACCGGACGTTTCGCCCACATCCGCTTTCCGGCGCTGGCCTGGGGCGAGAAGAATGGTACGGTCACCAACTCG
GAGCGGCGGATATCCCGTCAGCGCAGCTTTCTGCCGCCGCCGGGGGAGGCGAAAGCGGACTGGTGGATCATCGCCAGAGT
GGGCCAGGCGCTGGGGTATCGCGAGGCGTTTGCCTGGCAGCATCCGCATGACGTCTTCCGCGAGCATGCGGCGCTGTCCG
GTTTCGAAAACGACGGCCAGCGGGCGTTTGATATCGGCGCGCTGGCGGATCTCAGCCGTGAGGCGTGGGACGCCATGCCG
CCGGTGCGCTGGCCGGTCAGCCGCAGCGAGGCCGCCTGGGATATCACGCGCGGCTGGCATGGCGATGGCAGGCTGCGGAT
GGTGCCGGTGACGCCTCAACCGACCCGGGCGACAACCGATGCCTTTTATCCGCTGATCCTCAACAGCGGGCGGATCCGCG
ATCAGTGGCACACCATGACCCGTACCGGCGCGGTGCCGCGGCTGATGCAGCATATCGCGGAGCCAATGGTGGAGGTGGCG
CCGCAGGATGCCGTTCGCTATCAGCTTCCCGCTGACGGGCTGGCGCGGATCTGGTCGCGGCATGGGGTGATGGTGGCGAA
AGTGGCGATCAGCGAAGGGCAGCGCCCCGGCTCGCTGTTTGTTCCGATGCACTGGAACAACCAGTTTGCCCGTCAGGGGC
GGGTGAATAACCTGCTGGCCGCCGTCACGGACCCGTATTCCGGCCAGCCGGAAAGTAAACAGGCGGCGGTGGCGATCGCC
GCCTGGCAACCGGCCTGGCACAGCGAGCTTTTTTGCCGCGAACCGCTACCATTTCCTGCGGCCTGGCATTGGCGGCGGCG
GGCGACGCCAGGCGTGCTGCACTACTCGCTGGCCGGGGAGGCATCTGCCCGACAGTGGCTCAGCGCGTGGTGCGCCCGGC
GCGGCTGGCAGTTACAGGTGGCTGACGGCGGCGCGGTGTGGAACCTGCTGGCCTGGCATCAGGGAAGGCTGATGCTCGGC
TGGTGGAGCGATGCGCGCGAGCCGGCGGTGGATTGCGCATGGATAAGCGCTGCCTTTGCCGCGCCGCCGTCCGATGCTGC
CCAACGACATGCCTTGCTTAGCGGCCGACCGGGCGCCGCCGTCGCGCCGCGGGGGCGGATCGTCTGCAGCTGCTTTGGGG
TGGGGGAATGGTCAATTAACGAGGCCATCGCCAGCGGCTGCGCCAGCGTCGGGGCGCTGGGTGGGAAGCTGAAATGCGGG
ACCAACTGCGGCTCTTGCGTGCCGGAGCTCAACGCGCTGCTGGCGGCGCAACGAACCCGGGCGTAA

Upstream 100 bases:

>100_bases
CCAACGTGCTGTCGCGTGGGCTGTTAGGGGACGTCGGCGGCGAGCCGGTGGTGATCTCCCCGCTGTACAAACAGCGGATA
CGCCTGCGCGACGGCTGGCC

Downstream 100 bases:

>100_bases
CGGTGGCGCGCAGCGACGCCTCCGGTTGGGCGGCACCTTACCCAACCGGTTTCAGCTTATTCCTGGAATTGCCCACAGGG
CTGCGGGAGCAGGGAAAAAA

Product: putative nitrate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 901; Mature: 901

Protein sequence:

>901_residues
MRRRRTGGTRLAGESGKRQSVGGQPAAAGPRGGLLMKTIRTTCPYCGVGCGVLASVDDAGQVSVRGDDQHPANLGRLCVK
GAALGETTGLAGRLLTPEVDGQQVAWPQALAETAARLRQIIDQHGPQAVAFYASGQLLTEDYYAANKLMKGFIGAANIDT
NSRLCMSSAVTGYKRAFGADVVPCSYDDVENSDLVVLVGSNAAWAHPVLFQRLAQAKRDNPRLRIVAIDPRRTATCEIAD
RHLALAPGSDGGLFAGLLNALAEAGACVDGFRDGPQALAAARGWDVARVAAFCGLPADEVAGFYREFIAAPRAITLYTMG
INQSASGSDKCNAIINVHLASGKYGRRGCGPFSLTGQPNAMGGREVGGLATMLAAHMDFVPDDLQRLARFWGTERLAQTP
GLTAVELFAAIGRGEVKAVWIMGTNPVVSLPDSHAVSQALAACPLVIVSDVAAQTDTGRFAHIRFPALAWGEKNGTVTNS
ERRISRQRSFLPPPGEAKADWWIIARVGQALGYREAFAWQHPHDVFREHAALSGFENDGQRAFDIGALADLSREAWDAMP
PVRWPVSRSEAAWDITRGWHGDGRLRMVPVTPQPTRATTDAFYPLILNSGRIRDQWHTMTRTGAVPRLMQHIAEPMVEVA
PQDAVRYQLPADGLARIWSRHGVMVAKVAISEGQRPGSLFVPMHWNNQFARQGRVNNLLAAVTDPYSGQPESKQAAVAIA
AWQPAWHSELFCREPLPFPAAWHWRRRATPGVLHYSLAGEASARQWLSAWCARRGWQLQVADGGAVWNLLAWHQGRLMLG
WWSDAREPAVDCAWISAAFAAPPSDAAQRHALLSGRPGAAVAPRGRIVCSCFGVGEWSINEAIASGCASVGALGGKLKCG
TNCGSCVPELNALLAAQRTRA

Sequences:

>Translated_901_residues
MRRRRTGGTRLAGESGKRQSVGGQPAAAGPRGGLLMKTIRTTCPYCGVGCGVLASVDDAGQVSVRGDDQHPANLGRLCVK
GAALGETTGLAGRLLTPEVDGQQVAWPQALAETAARLRQIIDQHGPQAVAFYASGQLLTEDYYAANKLMKGFIGAANIDT
NSRLCMSSAVTGYKRAFGADVVPCSYDDVENSDLVVLVGSNAAWAHPVLFQRLAQAKRDNPRLRIVAIDPRRTATCEIAD
RHLALAPGSDGGLFAGLLNALAEAGACVDGFRDGPQALAAARGWDVARVAAFCGLPADEVAGFYREFIAAPRAITLYTMG
INQSASGSDKCNAIINVHLASGKYGRRGCGPFSLTGQPNAMGGREVGGLATMLAAHMDFVPDDLQRLARFWGTERLAQTP
GLTAVELFAAIGRGEVKAVWIMGTNPVVSLPDSHAVSQALAACPLVIVSDVAAQTDTGRFAHIRFPALAWGEKNGTVTNS
ERRISRQRSFLPPPGEAKADWWIIARVGQALGYREAFAWQHPHDVFREHAALSGFENDGQRAFDIGALADLSREAWDAMP
PVRWPVSRSEAAWDITRGWHGDGRLRMVPVTPQPTRATTDAFYPLILNSGRIRDQWHTMTRTGAVPRLMQHIAEPMVEVA
PQDAVRYQLPADGLARIWSRHGVMVAKVAISEGQRPGSLFVPMHWNNQFARQGRVNNLLAAVTDPYSGQPESKQAAVAIA
AWQPAWHSELFCREPLPFPAAWHWRRRATPGVLHYSLAGEASARQWLSAWCARRGWQLQVADGGAVWNLLAWHQGRLMLG
WWSDAREPAVDCAWISAAFAAPPSDAAQRHALLSGRPGAAVAPRGRIVCSCFGVGEWSINEAIASGCASVGALGGKLKCG
TNCGSCVPELNALLAAQRTRA
>Mature_901_residues
MRRRRTGGTRLAGESGKRQSVGGQPAAAGPRGGLLMKTIRTTCPYCGVGCGVLASVDDAGQVSVRGDDQHPANLGRLCVK
GAALGETTGLAGRLLTPEVDGQQVAWPQALAETAARLRQIIDQHGPQAVAFYASGQLLTEDYYAANKLMKGFIGAANIDT
NSRLCMSSAVTGYKRAFGADVVPCSYDDVENSDLVVLVGSNAAWAHPVLFQRLAQAKRDNPRLRIVAIDPRRTATCEIAD
RHLALAPGSDGGLFAGLLNALAEAGACVDGFRDGPQALAAARGWDVARVAAFCGLPADEVAGFYREFIAAPRAITLYTMG
INQSASGSDKCNAIINVHLASGKYGRRGCGPFSLTGQPNAMGGREVGGLATMLAAHMDFVPDDLQRLARFWGTERLAQTP
GLTAVELFAAIGRGEVKAVWIMGTNPVVSLPDSHAVSQALAACPLVIVSDVAAQTDTGRFAHIRFPALAWGEKNGTVTNS
ERRISRQRSFLPPPGEAKADWWIIARVGQALGYREAFAWQHPHDVFREHAALSGFENDGQRAFDIGALADLSREAWDAMP
PVRWPVSRSEAAWDITRGWHGDGRLRMVPVTPQPTRATTDAFYPLILNSGRIRDQWHTMTRTGAVPRLMQHIAEPMVEVA
PQDAVRYQLPADGLARIWSRHGVMVAKVAISEGQRPGSLFVPMHWNNQFARQGRVNNLLAAVTDPYSGQPESKQAAVAIA
AWQPAWHSELFCREPLPFPAAWHWRRRATPGVLHYSLAGEASARQWLSAWCARRGWQLQVADGGAVWNLLAWHQGRLMLG
WWSDAREPAVDCAWISAAFAAPPSDAAQRHALLSGRPGAAVAPRGRIVCSCFGVGEWSINEAIASGCASVGALGGKLKCG
TNCGSCVPELNALLAAQRTRA

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=799, Percent_Identity=27.6595744680851, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI3868721, Length=708, Percent_Identity=26.1299435028249, Blast_Score=232, Evalue=7e-62,
Organism=Escherichia coli, GI3868720, Length=632, Percent_Identity=24.5253164556962, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI1787778, Length=590, Percent_Identity=25.2542372881356, Blast_Score=107, Evalue=5e-24,
Organism=Escherichia coli, GI3868719, Length=256, Percent_Identity=30.46875, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR007419
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 96671; Mature: 96671

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRRRTGGTRLAGESGKRQSVGGQPAAAGPRGGLLMKTIRTTCPYCGVGCGVLASVDDAG
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEECCCCCC
QVSVRGDDQHPANLGRLCVKGAALGETTGLAGRLLTPEVDGQQVAWPQALAETAARLRQI
EEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHH
IDQHGPQAVAFYASGQLLTEDYYAANKLMKGFIGAANIDTNSRLCMSSAVTGYKRAFGAD
HHHCCCCEEEEEECCCEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
VVPCSYDDVENSDLVVLVGSNAAWAHPVLFQRLAQAKRDNPRLRIVAIDPRRTATCEIAD
CCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEC
RHLALAPGSDGGLFAGLLNALAEAGACVDGFRDGPQALAAARGWDVARVAAFCGLPADEV
CEEEECCCCCCCHHHHHHHHHHHCCCHHHCCCCCHHHHHHHCCCCHHHHHHHHCCCHHHH
AGFYREFIAAPRAITLYTMGINQSASGSDKCNAIINVHLASGKYGRRGCGPFSLTGQPNA
HHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCC
MGGREVGGLATMLAAHMDFVPDDLQRLARFWGTERLAQTPGLTAVELFAAIGRGEVKAVW
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHCCCCEEEEE
IMGTNPVVSLPDSHAVSQALAACPLVIVSDVAAQTDTGRFAHIRFPALAWGEKNGTVTNS
EEECCCEEECCCHHHHHHHHHHCHHHHHHHHHHCCCCCCEEEEECCEEEECCCCCCCCCH
ERRISRQRSFLPPPGEAKADWWIIARVGQALGYREAFAWQHPHDVFREHAALSGFENDGQ
HHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHCHHHHHCCCCCHHHHHHHHHHCCCCCCCC
RAFDIGALADLSREAWDAMPPVRWPVSRSEAAWDITRGWHGDGRLRMVPVTPQPTRATTD
EEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEECCCCCCCCCHH
AFYPLILNSGRIRDQWHTMTRTGAVPRLMQHIAEPMVEVAPQDAVRYQLPADGLARIWSR
HEEHEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHH
HGVMVAKVAISEGQRPGSLFVPMHWNNQFARQGRVNNLLAAVTDPYSGQPESKQAAVAIA
CCEEEEEEEECCCCCCCEEEEEEECCCHHHHCCCHHHHHEEECCCCCCCCCCCCEEEEEE
AWQPAWHSELFCREPLPFPAAWHWRRRATPGVLHYSLAGEASARQWLSAWCARRGWQLQV
ECCCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEE
ADGGAVWNLLAWHQGRLMLGWWSDAREPAVDCAWISAAFAAPPSDAAQRHALLSGRPGAA
ECCCCEEHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
VAPRGRIVCSCFGVGEWSINEAIASGCASVGALGGKLKCGTNCGSCVPELNALLAAQRTR
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCC
A
C
>Mature Secondary Structure
MRRRRTGGTRLAGESGKRQSVGGQPAAAGPRGGLLMKTIRTTCPYCGVGCGVLASVDDAG
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEECCCCCC
QVSVRGDDQHPANLGRLCVKGAALGETTGLAGRLLTPEVDGQQVAWPQALAETAARLRQI
EEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHH
IDQHGPQAVAFYASGQLLTEDYYAANKLMKGFIGAANIDTNSRLCMSSAVTGYKRAFGAD
HHHCCCCEEEEEECCCEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
VVPCSYDDVENSDLVVLVGSNAAWAHPVLFQRLAQAKRDNPRLRIVAIDPRRTATCEIAD
CCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEC
RHLALAPGSDGGLFAGLLNALAEAGACVDGFRDGPQALAAARGWDVARVAAFCGLPADEV
CEEEECCCCCCCHHHHHHHHHHHCCCHHHCCCCCHHHHHHHCCCCHHHHHHHHCCCHHHH
AGFYREFIAAPRAITLYTMGINQSASGSDKCNAIINVHLASGKYGRRGCGPFSLTGQPNA
HHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCC
MGGREVGGLATMLAAHMDFVPDDLQRLARFWGTERLAQTPGLTAVELFAAIGRGEVKAVW
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHCCCCEEEEE
IMGTNPVVSLPDSHAVSQALAACPLVIVSDVAAQTDTGRFAHIRFPALAWGEKNGTVTNS
EEECCCEEECCCHHHHHHHHHHCHHHHHHHHHHCCCCCCEEEEECCEEEECCCCCCCCCH
ERRISRQRSFLPPPGEAKADWWIIARVGQALGYREAFAWQHPHDVFREHAALSGFENDGQ
HHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHCHHHHHCCCCCHHHHHHHHHHCCCCCCCC
RAFDIGALADLSREAWDAMPPVRWPVSRSEAAWDITRGWHGDGRLRMVPVTPQPTRATTD
EEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEECCCCCCCCCHH
AFYPLILNSGRIRDQWHTMTRTGAVPRLMQHIAEPMVEVAPQDAVRYQLPADGLARIWSR
HEEHEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHH
HGVMVAKVAISEGQRPGSLFVPMHWNNQFARQGRVNNLLAAVTDPYSGQPESKQAAVAIA
CCEEEEEEEECCCCCCCEEEEEEECCCHHHHCCCHHHHHEEECCCCCCCCCCCCEEEEEE
AWQPAWHSELFCREPLPFPAAWHWRRRATPGVLHYSLAGEASARQWLSAWCARRGWQLQV
ECCCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEE
ADGGAVWNLLAWHQGRLMLGWWSDAREPAVDCAWISAAFAAPPSDAAQRHALLSGRPGAA
ECCCCEEHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
VAPRGRIVCSCFGVGEWSINEAIASGCASVGALGGKLKCGTNCGSCVPELNALLAAQRTR
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]