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The map label for this gene is narK [H]

Identifier: 238895275

GI number: 238895275

Start: 3154960

End: 3156348

Strand: Reverse

Name: narK [H]

Synonym: KP1_3327

Alternate gene names: 238895275

Gene position: 3156348-3154960 (Counterclockwise)

Preceding gene: 238895278

Following gene: 238895274

Centisome position: 60.14

GC content: 59.25

Gene sequence:

>1389_bases
ATGAGTCAATCTTCCCTCCCGGAGAAGGCTAACCGCTCGGTCATAACCGACTGGCGGCCTGAAGATCCTGAATTCTGGCA
ACAGCGCGGCCATCGTGTGGCAAGCCGCAATTTATGGATCTCCGTTCCCTGTCTGTTACTGGCGTTCTGCGTTTGGATGT
TGTTCAGCGCGGTTGCCGTTAACCTCAACAAAGTAGGCTTTCAGTTTACAACCGATCAGCTGTTTATGCTGACCGCGCTG
CCGGCGCTGTCAGGCGCCTTATTACGCGTGCCTTACGCCTTTATGGTGCCGCTGTTCGGCGGCCGTCGCTGGACGGCGTT
CAGCACCGGGATCATGATTGTGCCGTGCGTCTGGCTGGGCTTCGCGGTCCAGGATACCTCTACGCCGTTTAGCATCTTCG
TGATTATCTCTCTGCTGTGCGGCTTCGCCGGAGCCAACTTTGCCTCCAGTATGGCCAACATCAGCTTCTTCTTCCCGAAA
GCGAAGCAGGGCGGGGCGCTCGGCGTTAACGGCGGCCTCGGCAATATGGGCGTCAGCGTGATGCAGCTGGTCGCGCCGCT
GGTGGTCTCGATCTCTATCTTTGCCGTTTTTGGCGGTAACGGTAGCGAGCAACCGGACGGCTCGATGCTGTATCTGGAAA
ACGCCGCGTGGATCTGGGTGCCTTTCCTGATTATTTTCACTCTGGCGGCGTGGTTCTTTATGAACGACCTGTCGGCTTCG
AAAGCGTCGCTGAGCGAGCAGTTGCCGGTACTTAAGCGCCTGCATCTGTGGATTATGGCGCTGCTCTATCTCGCGACCTT
CGGCTCCTTTATCGGCTTTTCCGCCGGGTTCGCGATGCTGTCAAAAACTCAGTTCCCGGATGTGCAGATCCTGCATTACG
CCTTCTTCGGCCCGTTCATTGGCGCGCTGGCGCGTTCGATGGGCGGGGCGATCTCCGACCGCCTCGGTGGGACCCGCGTA
ACGCTGGTCAACTTTGTGGTGATGGCTGTCTTCTGCGCGCTGCTGTTCCTGACCCTGCCGACCAACGGCCAGGGCGGTAA
CTTTATCGCCTTCTTCGCGGTGTTTATGGTGCTGTTCCTGACCGCCGGGCTGGGGAGCGCCTCCACCTTCCAGATGATCT
CCGTGATCTTCCGTAAGCTGACTATGGACCGCGTGAAGGCCCAGGGCGGCAGCGAAGCGCAGGCGATGCGCGAGGCGGCG
ACCGATACCGCGGCGGCGCTGGGCTTTATCTCCGCCATTGGCGCTATCGGCGGCTTCTTTATTCCGAAAGCGTTCGGTAT
CTCGCTGGATCTGACCGGCTCGCCGGCCGGGGCGATGAAAGTGTTCCTCGTGTTCTATATTGCCTGCGTGGTGATCACCT
GGGCGGTATACGGTCGTAAGCGTCAGTAA

Upstream 100 bases:

>100_bases
TGCACAAATTAGCTCGCGCTTTTCCTTTGATTTATATCAACTTACCGCCCCGGGTAACCCCTAAGGTAGCGGCAGGAAAT
ATGAATAATCAGAGGTATCT

Downstream 100 bases:

>100_bases
TCGGCCGCGGTAACGTTGGCGTATAAAAAGGCGCGCTCCCCACGGAGGCGCCTTTTTTAACCGCTCAATTAGTTACAACA
TACTATGGGAGAATATTGTC

Product: nitrite extrusion protein

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSQSSLPEKANRSVITDWRPEDPEFWQQRGHRVASRNLWISVPCLLLAFCVWMLFSAVAVNLNKVGFQFTTDQLFMLTAL
PALSGALLRVPYAFMVPLFGGRRWTAFSTGIMIVPCVWLGFAVQDTSTPFSIFVIISLLCGFAGANFASSMANISFFFPK
AKQGGALGVNGGLGNMGVSVMQLVAPLVVSISIFAVFGGNGSEQPDGSMLYLENAAWIWVPFLIIFTLAAWFFMNDLSAS
KASLSEQLPVLKRLHLWIMALLYLATFGSFIGFSAGFAMLSKTQFPDVQILHYAFFGPFIGALARSMGGAISDRLGGTRV
TLVNFVVMAVFCALLFLTLPTNGQGGNFIAFFAVFMVLFLTAGLGSASTFQMISVIFRKLTMDRVKAQGGSEAQAMREAA
TDTAAALGFISAIGAIGGFFIPKAFGISLDLTGSPAGAMKVFLVFYIACVVITWAVYGRKRQ

Sequences:

>Translated_462_residues
MSQSSLPEKANRSVITDWRPEDPEFWQQRGHRVASRNLWISVPCLLLAFCVWMLFSAVAVNLNKVGFQFTTDQLFMLTAL
PALSGALLRVPYAFMVPLFGGRRWTAFSTGIMIVPCVWLGFAVQDTSTPFSIFVIISLLCGFAGANFASSMANISFFFPK
AKQGGALGVNGGLGNMGVSVMQLVAPLVVSISIFAVFGGNGSEQPDGSMLYLENAAWIWVPFLIIFTLAAWFFMNDLSAS
KASLSEQLPVLKRLHLWIMALLYLATFGSFIGFSAGFAMLSKTQFPDVQILHYAFFGPFIGALARSMGGAISDRLGGTRV
TLVNFVVMAVFCALLFLTLPTNGQGGNFIAFFAVFMVLFLTAGLGSASTFQMISVIFRKLTMDRVKAQGGSEAQAMREAA
TDTAAALGFISAIGAIGGFFIPKAFGISLDLTGSPAGAMKVFLVFYIACVVITWAVYGRKRQ
>Mature_461_residues
SQSSLPEKANRSVITDWRPEDPEFWQQRGHRVASRNLWISVPCLLLAFCVWMLFSAVAVNLNKVGFQFTTDQLFMLTALP
ALSGALLRVPYAFMVPLFGGRRWTAFSTGIMIVPCVWLGFAVQDTSTPFSIFVIISLLCGFAGANFASSMANISFFFPKA
KQGGALGVNGGLGNMGVSVMQLVAPLVVSISIFAVFGGNGSEQPDGSMLYLENAAWIWVPFLIIFTLAAWFFMNDLSASK
ASLSEQLPVLKRLHLWIMALLYLATFGSFIGFSAGFAMLSKTQFPDVQILHYAFFGPFIGALARSMGGAISDRLGGTRVT
LVNFVVMAVFCALLFLTLPTNGQGGNFIAFFAVFMVLFLTAGLGSASTFQMISVIFRKLTMDRVKAQGGSEAQAMREAAT
DTAAALGFISAIGAIGGFFIPKAFGISLDLTGSPAGAMKVFLVFYIACVVITWAVYGRKRQ

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=462, Percent_Identity=85.9307359307359, Blast_Score=777, Evalue=0.0,
Organism=Escherichia coli, GI1787743, Length=454, Percent_Identity=73.568281938326, Blast_Score=650, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49914; Mature: 49783

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSSLPEKANRSVITDWRPEDPEFWQQRGHRVASRNLWISVPCLLLAFCVWMLFSAVAV
CCCCCCCCHHCCCCCCCCCCCCHHHHHHCCCEEHHCCCEEEHHHHHHHHHHHHHHHHHHH
NLNKVGFQFTTDQLFMLTALPALSGALLRVPYAFMVPLFGGRRWTAFSTGIMIVPCVWLG
HHHHCCCEECCHHHHHHHHHHHHCCHHHHCCHHHHHHHCCCCEEEECCCCHHHHHHHHHH
FAVQDTSTPFSIFVIISLLCGFAGANFASSMANISFFFPKAKQGGALGVNGGLGNMGVSV
HEEECCCCCHHHHHHHHHHHHHCCCHHHHHHCCCEEECCCCCCCCCEECCCCCCHHHHHH
MQLVAPLVVSISIFAVFGGNGSEQPDGSMLYLENAAWIWVPFLIIFTLAAWFFMNDLSAS
HHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHH
KASLSEQLPVLKRLHLWIMALLYLATFGSFIGFSAGFAMLSKTQFPDVQILHYAFFGPFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GALARSMGGAISDRLGGTRVTLVNFVVMAVFCALLFLTLPTNGQGGNFIAFFAVFMVLFL
HHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
TAGLGSASTFQMISVIFRKLTMDRVKAQGGSEAQAMREAATDTAAALGFISAIGAIGGFF
HCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPKAFGISLDLTGSPAGAMKVFLVFYIACVVITWAVYGRKRQ
HHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SQSSLPEKANRSVITDWRPEDPEFWQQRGHRVASRNLWISVPCLLLAFCVWMLFSAVAV
CCCCCCCHHCCCCCCCCCCCCHHHHHHCCCEEHHCCCEEEHHHHHHHHHHHHHHHHHHH
NLNKVGFQFTTDQLFMLTALPALSGALLRVPYAFMVPLFGGRRWTAFSTGIMIVPCVWLG
HHHHCCCEECCHHHHHHHHHHHHCCHHHHCCHHHHHHHCCCCEEEECCCCHHHHHHHHHH
FAVQDTSTPFSIFVIISLLCGFAGANFASSMANISFFFPKAKQGGALGVNGGLGNMGVSV
HEEECCCCCHHHHHHHHHHHHHCCCHHHHHHCCCEEECCCCCCCCCEECCCCCCHHHHHH
MQLVAPLVVSISIFAVFGGNGSEQPDGSMLYLENAAWIWVPFLIIFTLAAWFFMNDLSAS
HHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHH
KASLSEQLPVLKRLHLWIMALLYLATFGSFIGFSAGFAMLSKTQFPDVQILHYAFFGPFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
GALARSMGGAISDRLGGTRVTLVNFVVMAVFCALLFLTLPTNGQGGNFIAFFAVFMVLFL
HHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
TAGLGSASTFQMISVIFRKLTMDRVKAQGGSEAQAMREAATDTAAALGFISAIGAIGGFF
HCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPKAFGISLDLTGSPAGAMKVFLVFYIACVVITWAVYGRKRQ
HHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]