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The map label for this gene is btuE [H]

Identifier: 238895222

GI number: 238895222

Start: 3103949

End: 3104500

Strand: Reverse

Name: btuE [H]

Synonym: KP1_3270

Alternate gene names: 238895222

Gene position: 3104500-3103949 (Counterclockwise)

Preceding gene: 238895224

Following gene: 238895221

Centisome position: 59.15

GC content: 56.88

Gene sequence:

>552_bases
ATGCAACATGACATTCTGAATACCGAAGTGACGACCATTGATGGCGAGAAAACCACCCTGGCATCCTTTGCCGGCAAGGT
GCTGCTGATCGTCAATGTAGCCTCGAAATGCGGCCTGACGCCGCAGTATGAGCAACTCGAGGATCTGCAAAAGCAGTTTG
CCGCCGAGGGCTTCAGCGTGCTGGGGTTCCCCTGCAACCAGTTCCTCGGCCAGGAGCCGGGCAGTGAAGAGGAGATTAAG
ACCTTCTGCAGCACTACCTACGGCGTCACCTTCCCGCTGTTTAGCAAAATTGACGTCAACGGCGAACATCGCGCGCCGCT
CTATCAAAAACTGATTGCCGCCGCGCCGAAAGCGGTGGCCCCGGAAGGCAGCGGTTTTTATGAGCGGATGGCCAGCAAAG
GCCGGGCACCGCTGTATGTGGACGACATTCTGTGGAATTTCGAAAAATTCCTCATCGATCGCCAGGGCAACGTCATTCAG
CGCTTCTCTCCGGATATGACGCCGGACGATCCGCAGCTGGTGGCGGCGATTAAAGGAGCGCTGGCGCAATAA

Upstream 100 bases:

>100_bases
CAACTCGGGGGGAATGGCAGTAGAATGGCGGGCGCTGAATCCGCTACAGTAGAAAGGGATCACCTTCACCGGAAACCGGT
TTATCCATTGAGGACAGGCT

Downstream 100 bases:

>100_bases
TGTCGTTTCTGATGCAGCTACAGGATGTAGAAGCGGCGGGGCGCCTGGCCCCGTTTTCTGCGGCCTTTCGCGCCGGGGAG
ATTGTCCATCTGGTGGGGCC

Product: putative glutathione peroxidase

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MQHDILNTEVTTIDGEKTTLASFAGKVLLIVNVASKCGLTPQYEQLEDLQKQFAAEGFSVLGFPCNQFLGQEPGSEEEIK
TFCSTTYGVTFPLFSKIDVNGEHRAPLYQKLIAAAPKAVAPEGSGFYERMASKGRAPLYVDDILWNFEKFLIDRQGNVIQ
RFSPDMTPDDPQLVAAIKGALAQ

Sequences:

>Translated_183_residues
MQHDILNTEVTTIDGEKTTLASFAGKVLLIVNVASKCGLTPQYEQLEDLQKQFAAEGFSVLGFPCNQFLGQEPGSEEEIK
TFCSTTYGVTFPLFSKIDVNGEHRAPLYQKLIAAAPKAVAPEGSGFYERMASKGRAPLYVDDILWNFEKFLIDRQGNVIQ
RFSPDMTPDDPQLVAAIKGALAQ
>Mature_183_residues
MQHDILNTEVTTIDGEKTTLASFAGKVLLIVNVASKCGLTPQYEQLEDLQKQFAAEGFSVLGFPCNQFLGQEPGSEEEIK
TFCSTTYGVTFPLFSKIDVNGEHRAPLYQKLIAAAPKAVAPEGSGFYERMASKGRAPLYVDDILWNFEKFLIDRQGNVIQ
RFSPDMTPDDPQLVAAIKGALAQ

Specific function: Not essential for B12 transport; however, it is an auxiliary component of the transport system [H]

COG id: COG0386

COG function: function code O; Glutathione peroxidase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutathione peroxidase family [H]

Homologues:

Organism=Homo sapiens, GI75709200, Length=157, Percent_Identity=41.4012738853503, Blast_Score=114, Evalue=5e-26,
Organism=Homo sapiens, GI90903240, Length=157, Percent_Identity=41.4012738853503, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI15618997, Length=180, Percent_Identity=34.4444444444444, Blast_Score=110, Evalue=8e-25,
Organism=Homo sapiens, GI192455698, Length=175, Percent_Identity=34.2857142857143, Blast_Score=103, Evalue=9e-23,
Organism=Homo sapiens, GI6006001, Length=171, Percent_Identity=39.766081871345, Blast_Score=100, Evalue=5e-22,
Organism=Homo sapiens, GI4557629, Length=157, Percent_Identity=39.4904458598726, Blast_Score=100, Evalue=7e-22,
Organism=Homo sapiens, GI90903238, Length=139, Percent_Identity=41.0071942446043, Blast_Score=96, Evalue=1e-20,
Organism=Homo sapiens, GI41406084, Length=172, Percent_Identity=37.2093023255814, Blast_Score=94, Evalue=6e-20,
Organism=Homo sapiens, GI33186887, Length=154, Percent_Identity=40.9090909090909, Blast_Score=93, Evalue=1e-19,
Organism=Homo sapiens, GI32967607, Length=160, Percent_Identity=37.5, Blast_Score=85, Evalue=4e-17,
Organism=Escherichia coli, GI1788003, Length=183, Percent_Identity=75.9562841530055, Blast_Score=289, Evalue=7e-80,
Organism=Caenorhabditis elegans, GI17535473, Length=162, Percent_Identity=45.0617283950617, Blast_Score=129, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17506887, Length=180, Percent_Identity=39.4444444444444, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17550320, Length=167, Percent_Identity=37.125748502994, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI193209493, Length=167, Percent_Identity=35.3293413173653, Blast_Score=110, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71993584, Length=171, Percent_Identity=35.0877192982456, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI193210707, Length=165, Percent_Identity=35.1515151515151, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71993573, Length=98, Percent_Identity=36.734693877551, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71999642, Length=75, Percent_Identity=36, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319721, Length=168, Percent_Identity=44.6428571428571, Blast_Score=147, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6322228, Length=154, Percent_Identity=47.4025974025974, Blast_Score=145, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6322826, Length=155, Percent_Identity=38.7096774193548, Blast_Score=117, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24656772, Length=163, Percent_Identity=37.4233128834356, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24656777, Length=163, Percent_Identity=37.4233128834356, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24656767, Length=163, Percent_Identity=37.4233128834356, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI85726443, Length=166, Percent_Identity=37.9518072289157, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000889
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00255 GSHPx [H]

EC number: NA

Molecular weight: Translated: 20123; Mature: 20123

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00460 GLUTATHIONE_PEROXID_1 ; PS00763 GLUTATHIONE_PEROXID_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHDILNTEVTTIDGEKTTLASFAGKVLLIVNVASKCGLTPQYEQLEDLQKQFAAEGFSV
CCCCCCCCEEEEECCCHHHHHHHHCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHCCCEE
LGFPCNQFLGQEPGSEEEIKTFCSTTYGVTFPLFSKIDVNGEHRAPLYQKLIAAAPKAVA
ECCCHHHHHCCCCCCHHHHHHHHHHHCCCEECCCEEECCCCCCCCHHHHHHHHHCCCCCC
PEGSGFYERMASKGRAPLYVDDILWNFEKFLIDRQGNVIQRFSPDMTPDDPQLVAAIKGA
CCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHH
LAQ
HCC
>Mature Secondary Structure
MQHDILNTEVTTIDGEKTTLASFAGKVLLIVNVASKCGLTPQYEQLEDLQKQFAAEGFSV
CCCCCCCCEEEEECCCHHHHHHHHCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHCCCEE
LGFPCNQFLGQEPGSEEEIKTFCSTTYGVTFPLFSKIDVNGEHRAPLYQKLIAAAPKAVA
ECCCHHHHHCCCCCCHHHHHHHHHHHCCCEECCCEEECCCCCCCCHHHHHHHHHCCCCCC
PEGSGFYERMASKGRAPLYVDDILWNFEKFLIDRQGNVIQRFSPDMTPDDPQLVAAIKGA
CCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHH
LAQ
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3528129; 9097039; 9278503 [H]