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The map label for this gene is hisJ [H]

Identifier: 238895147

GI number: 238895147

Start: 3027618

End: 3028385

Strand: Reverse

Name: hisJ [H]

Synonym: KP1_3189

Alternate gene names: 238895147

Gene position: 3028385-3027618 (Counterclockwise)

Preceding gene: 238895148

Following gene: 238895146

Centisome position: 57.7

GC content: 56.12

Gene sequence:

>768_bases
ATGGTCAAGAATTTGCTCAAAGCGTGTTGCATGATAGCCGCGCTGACGGCTGCCGGACAGGCGGCTGCGGAAACCTATAC
CGTCGGCTCCGGCGGCACCTACCGGCCGTTTGAATTTGAAAATAGCCAGAAACAGCTGGAAGGTTTCGACATCGACATCA
TCAAAGCCATCGCCAAAGCCGAAGGCTTTGACGTTAAGCTGGTCAATACCCCATGGGAAGGCATCTTCGCCACGCTGAAC
ACCGGCGACCGCGACATCATCATCTCCGGGATCACCATCACCGATAAACGCAAACAGATGGTCGATTTCTCCGCGCCGTA
CTTCCCGGCCGAACAGTCTATCGTCGTCGCTCAGGACTCGCAGGTGGATTCGCTGGCGGCCCTGAAAAACGAGAAGGTCG
GGGTGGTGAACTCCAGCACCGGCGATATCGTGGTTTCTGAGGTGCTGGGTAAAAACAGCACCGCGATCAAGCGCTTCGAC
AATACCCCGCTGATGCTCCAGGAGCTGTTTGAGGACGGCGTCAGCGCCGCGGTTGGCGACGTCGGGGTGGTGAAGTACTA
CATCAAACAGCACCCGGAGAAGCAGTTCAAGCTGGTGCCGGATGCCAAATTTGAGCGTCAGTATTTCGGTATTGCGGTGG
CGAAAGGCAACAGCGAACTGCTGGGCAAAATCAACGCCGGGCTGCAAAAGATCGTTGCTGACGGGACTTACGCCAAAATC
TATAAAACCTGGTTCGACGACAACGTGCCGACGCTGCCAGCGCAATAA

Upstream 100 bases:

>100_bases
GCCATTGAGAAGATAATTCACCAGCGGGAAGGTTTCCGGTTGCATATAAAAGTGAGTGTGTGTATTTTTATGCAAAATAA
ATGCATAAAAAGGAAATCTC

Downstream 100 bases:

>100_bases
TCCACCTTTTTCAGTGACCACGGCTATCGCCCAGGCGATAGCCGTATTTTTTAGGGAAGGACTATGACGGGATTCCGTTG
GGAGATCATTGAGGAGTACG

Product: amino acid ABC transport system periplasmic binding component

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MVKNLLKACCMIAALTAAGQAAAETYTVGSGGTYRPFEFENSQKQLEGFDIDIIKAIAKAEGFDVKLVNTPWEGIFATLN
TGDRDIIISGITITDKRKQMVDFSAPYFPAEQSIVVAQDSQVDSLAALKNEKVGVVNSSTGDIVVSEVLGKNSTAIKRFD
NTPLMLQELFEDGVSAAVGDVGVVKYYIKQHPEKQFKLVPDAKFERQYFGIAVAKGNSELLGKINAGLQKIVADGTYAKI
YKTWFDDNVPTLPAQ

Sequences:

>Translated_255_residues
MVKNLLKACCMIAALTAAGQAAAETYTVGSGGTYRPFEFENSQKQLEGFDIDIIKAIAKAEGFDVKLVNTPWEGIFATLN
TGDRDIIISGITITDKRKQMVDFSAPYFPAEQSIVVAQDSQVDSLAALKNEKVGVVNSSTGDIVVSEVLGKNSTAIKRFD
NTPLMLQELFEDGVSAAVGDVGVVKYYIKQHPEKQFKLVPDAKFERQYFGIAVAKGNSELLGKINAGLQKIVADGTYAKI
YKTWFDDNVPTLPAQ
>Mature_255_residues
MVKNLLKACCMIAALTAAGQAAAETYTVGSGGTYRPFEFENSQKQLEGFDIDIIKAIAKAEGFDVKLVNTPWEGIFATLN
TGDRDIIISGITITDKRKQMVDFSAPYFPAEQSIVVAQDSQVDSLAALKNEKVGVVNSSTGDIVVSEVLGKNSTAIKRFD
NTPLMLQELFEDGVSAAVGDVGVVKYYIKQHPEKQFKLVPDAKFERQYFGIAVAKGNSELLGKINAGLQKIVADGTYAKI
YKTWFDDNVPTLPAQ

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=249, Percent_Identity=35.7429718875502, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787088, Length=243, Percent_Identity=32.5102880658436, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787085, Length=243, Percent_Identity=32.5102880658436, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788649, Length=257, Percent_Identity=29.5719844357977, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1788648, Length=228, Percent_Identity=30.2631578947368, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788228, Length=228, Percent_Identity=29.8245614035088, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27700; Mature: 27700

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKNLLKACCMIAALTAAGQAAAETYTVGSGGTYRPFEFENSQKQLEGFDIDIIKAIAKA
CHHHHHHHHHHHHHHHHCCCHHHCEEEECCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHC
EGFDVKLVNTPWEGIFATLNTGDRDIIISGITITDKRKQMVDFSAPYFPAEQSIVVAQDS
CCCEEEEECCCCCCEEEEECCCCCEEEEEEEEEECCHHHHHHCCCCCCCCCCEEEEECCC
QVDSLAALKNEKVGVVNSSTGDIVVSEVLGKNSTAIKRFDNTPLMLQELFEDGVSAAVGD
CHHHHHHHCCCCEEEEECCCCCEEHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCC
VGVVKYYIKQHPEKQFKLVPDAKFERQYFGIAVAKGNSELLGKINAGLQKIVADGTYAKI
HHHHHHHHHHCCCCCEEECCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHH
YKTWFDDNVPTLPAQ
HHHHHCCCCCCCCCC
>Mature Secondary Structure
MVKNLLKACCMIAALTAAGQAAAETYTVGSGGTYRPFEFENSQKQLEGFDIDIIKAIAKA
CHHHHHHHHHHHHHHHHCCCHHHCEEEECCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHC
EGFDVKLVNTPWEGIFATLNTGDRDIIISGITITDKRKQMVDFSAPYFPAEQSIVVAQDS
CCCEEEEECCCCCCEEEEECCCCCEEEEEEEEEECCHHHHHHCCCCCCCCCCEEEEECCC
QVDSLAALKNEKVGVVNSSTGDIVVSEVLGKNSTAIKRFDNTPLMLQELFEDGVSAAVGD
CHHHHHHHCCCCEEEEECCCCCEEHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCC
VGVVKYYIKQHPEKQFKLVPDAKFERQYFGIAVAKGNSELLGKINAGLQKIVADGTYAKI
HHHHHHHHHHCCCCCEEECCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHH
YKTWFDDNVPTLPAQ
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]