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The map label for this gene is budR [H]

Identifier: 238895099

GI number: 238895099

Start: 2976950

End: 2977822

Strand: Reverse

Name: budR [H]

Synonym: KP1_3135

Alternate gene names: 238895099

Gene position: 2977822-2976950 (Counterclockwise)

Preceding gene: 238895104

Following gene: 238895092

Centisome position: 56.74

GC content: 61.4

Gene sequence:

>873_bases
ATGGAACTTCGTTATCTTCGCTATTTTGTCGCCGTCGCCAGGACGCGGCACTTCACCCAGGCCGCCAAGGAGCTGGGTAT
TTCACAGCCTCCGTTAAGTCAGCAAATTCAGCGGCTTGAGCGCGAAGTGGGGACCCCGCTGTTCCGGCGTTTAACCCGCG
GGGTGGAGCTGACGGAAGCGGGAGAGTCTTTCTATGAGGATGCCCGCCAGATCCTCGCCATGAGCGATGCGGCGCTGGAG
AAGGCCAAGGGCATTGCGCGCGGGATGAACGGCAGCCTGTCGCTAGGCATTACCAGTTCTGATGCTTTCCATCCGCAAAT
CTTCACGCTGCTGCACCGTTTTCAGCTCGACCACCCCGGGGTGACCCTGCACCAGATGGAAGACAATATGGCCAATCTGA
TGACCGCCCTGAGCGAGGCCGAGCTGGATATCGCCTTCGTCCGCCTGCCGTGCGAGAGCAGTAAAGCCTTTAATCTGCGG
ATTATCGATGAGGAGCCGATGGTTATCGCGCTGCCGCGCGATAACCCGCTGGCGACGCAGCCGACCCTTGCCCTGGAGCA
GCTGCGCGACGTGGCGCCGATCCTCTTCCCGCGGGAGGTGGCCCCCGGGCTGTATGAGCTGGTGTATAACAGTTGCCTGC
GCGCCGGGATCGATATGGAGCGCGCCTGGCAGTCGTCGCAAATTTCATCGTCGCTGAGCATGGTCAATGCAGGCTTCGGC
TTCGCGCTGGTTCCGCAGTCGATGACCTGCATCCAGCAGCCGAACGTCAGCTATCACCCGCTTAGCGGGGCGCCGCTGAA
GACCGATATCGCCATCGCCTGGCGGCGCTTCGAGCGCTCGCGTACGGTGAAACGCTTTCTGGCGATGTTCTGA

Upstream 100 bases:

>100_bases
CTTCCTCGTTCAACAAATATAAGAAAGGTTAAATAAATGTTGACCCGATTCAGCTCACAGTTCCAATATAGAAACCATGC
TGGTTTGAGACGTTTTCGAT

Downstream 100 bases:

>100_bases
TCTTCGCGGCGCCAGCGGCTTACTGCGCCACCCAGCCGCCATCCATGTTCCATGCGGCGCCGCGCACCTGGGCGGCGGCG
TCGCTGCACAGAAACAGTGC

Product: putative LysR-family transcriptional regulator

Products: NA

Alternate protein names: Bud operon transcriptional regulator [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MELRYLRYFVAVARTRHFTQAAKELGISQPPLSQQIQRLEREVGTPLFRRLTRGVELTEAGESFYEDARQILAMSDAALE
KAKGIARGMNGSLSLGITSSDAFHPQIFTLLHRFQLDHPGVTLHQMEDNMANLMTALSEAELDIAFVRLPCESSKAFNLR
IIDEEPMVIALPRDNPLATQPTLALEQLRDVAPILFPREVAPGLYELVYNSCLRAGIDMERAWQSSQISSSLSMVNAGFG
FALVPQSMTCIQQPNVSYHPLSGAPLKTDIAIAWRRFERSRTVKRFLAMF

Sequences:

>Translated_290_residues
MELRYLRYFVAVARTRHFTQAAKELGISQPPLSQQIQRLEREVGTPLFRRLTRGVELTEAGESFYEDARQILAMSDAALE
KAKGIARGMNGSLSLGITSSDAFHPQIFTLLHRFQLDHPGVTLHQMEDNMANLMTALSEAELDIAFVRLPCESSKAFNLR
IIDEEPMVIALPRDNPLATQPTLALEQLRDVAPILFPREVAPGLYELVYNSCLRAGIDMERAWQSSQISSSLSMVNAGFG
FALVPQSMTCIQQPNVSYHPLSGAPLKTDIAIAWRRFERSRTVKRFLAMF
>Mature_290_residues
MELRYLRYFVAVARTRHFTQAAKELGISQPPLSQQIQRLEREVGTPLFRRLTRGVELTEAGESFYEDARQILAMSDAALE
KAKGIARGMNGSLSLGITSSDAFHPQIFTLLHRFQLDHPGVTLHQMEDNMANLMTALSEAELDIAFVRLPCESSKAFNLR
IIDEEPMVIALPRDNPLATQPTLALEQLRDVAPILFPREVAPGLYELVYNSCLRAGIDMERAWQSSQISSSLSMVNAGFG
FALVPQSMTCIQQPNVSYHPLSGAPLKTDIAIAWRRFERSRTVKRFLAMF

Specific function: Regulator of the budABC operon for 2,3-butanediol synthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=290, Percent_Identity=34.8275862068965, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI2367136, Length=261, Percent_Identity=31.4176245210728, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1788887, Length=305, Percent_Identity=31.8032786885246, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1790399, Length=247, Percent_Identity=30.7692307692308, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI157672245, Length=290, Percent_Identity=29.3103448275862, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693105, Length=265, Percent_Identity=24.9056603773585, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787601, Length=145, Percent_Identity=35.1724137931034, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788297, Length=147, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790208, Length=172, Percent_Identity=31.3953488372093, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788748, Length=245, Percent_Identity=23.6734693877551, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789173, Length=135, Percent_Identity=35.5555555555556, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788481, Length=291, Percent_Identity=26.8041237113402, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787806, Length=293, Percent_Identity=25.2559726962457, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994962, Length=146, Percent_Identity=29.4520547945205, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788626, Length=250, Percent_Identity=28.8, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI87081904, Length=208, Percent_Identity=26.9230769230769, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32554; Mature: 32554

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRYLRYFVAVARTRHFTQAAKELGISQPPLSQQIQRLEREVGTPLFRRLTRGVELTEA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCHHHH
GESFYEDARQILAMSDAALEKAKGIARGMNGSLSLGITSSDAFHPQIFTLLHRFQLDHPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCC
VTLHQMEDNMANLMTALSEAELDIAFVRLPCESSKAFNLRIIDEEPMVIALPRDNPLATQ
CCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCEEEEECCCCCCCCC
PTLALEQLRDVAPILFPREVAPGLYELVYNSCLRAGIDMERAWQSSQISSSLSMVNAGFG
HHHHHHHHHHHHHHCCCHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
FALVPQSMTCIQQPNVSYHPLSGAPLKTDIAIAWRRFERSRTVKRFLAMF
EEECCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELRYLRYFVAVARTRHFTQAAKELGISQPPLSQQIQRLEREVGTPLFRRLTRGVELTEA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCHHHH
GESFYEDARQILAMSDAALEKAKGIARGMNGSLSLGITSSDAFHPQIFTLLHRFQLDHPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCC
VTLHQMEDNMANLMTALSEAELDIAFVRLPCESSKAFNLRIIDEEPMVIALPRDNPLATQ
CCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCEEEEECCCCCCCCC
PTLALEQLRDVAPILFPREVAPGLYELVYNSCLRAGIDMERAWQSSQISSSLSMVNAGFG
HHHHHHHHHHHHHHCCCHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
FALVPQSMTCIQQPNVSYHPLSGAPLKTDIAIAWRRFERSRTVKRFLAMF
EEECCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA