Click here to switch to the map view.

The map label for this gene is vanB [H]

Identifier: 238895078

GI number: 238895078

Start: 2960249

End: 2961217

Strand: Direct

Name: vanB [H]

Synonym: KP1_3110

Alternate gene names: 238895078

Gene position: 2960249-2961217 (Clockwise)

Preceding gene: 238895073

Following gene: 238895079

Centisome position: 56.4

GC content: 59.55

Gene sequence:

>969_bases
ATGCACAGCCACGATCTTATTTCGGTCTCGGTCGGGGAAATACGCCCCAACGGACAGGGAAACCTATCCATAATTTTGCA
GACGGCGGCCGGGGAAATATTACCGGCCTACTCCGCCGGGGCGCATATCGATATTATTATTCCCGGCGTCGGCCCGCGGC
AGTATTCCCTGTGCGGCACGCCTGACCGCAGTAATACTTATGAAATTTGCGTCAGACTGACTGACACCTCCACCGGCGGC
TCACGCTATTTGCACCAGCAGCTCAAAGCCGGCGATCGGCTCGCCATTTCGCCGCCGCGCAACCATTTTCCCCTGCCGCA
GGCCGGGCGCTACCTGCTATTTGCCGGCGGTATCGGCATCACGCCTTTGCTGGCGATGGCTGAAGCGATTGCCGCCAGAA
AGGGGGCGCTGGAGCTACATTATTATGTCGCCAATTCGCGCCAGACCGCCTTCTCCCCGCGCCTGAACCAGCTGACTGCG
AACGGCACGGTGGCCATTCACTGCAGCGAGGAAGGCGCGTCGTTTCGCCAGCGGGTCCCCGAATGCTTAACTGTGCACCA
CCCTGATACCGTAGTAATAGCCTGCGGCCCGGAGGGTTTTATTCAGCGACTGCGGTCGGTCATGGAGGAATATCGCTGGT
CGCCGTCGCAGTTCGTATTCGAGCGTTTTACTCCCGCGGCGGAAAATAATACTGCGGCGAAAAATGCGTTTTATATTGAG
CTGGCCTCCAGCGGGCAGCGCCTGCAGGTGGCCGCGGATCAAACCATTGCTCAGGTATTACAGCACGCCGGGGTGGAGGT
AATGCTCTCCTGCGAACAGGGAATGTGCGGCTCCTGTATTACAGGGGTCCTCGACGGGATCCCCGAGCACCGGGACAGCG
TGCTGACGGCGGAAGAGAAAGCCGGCAACGACCAGATCACGCTCTGCTGTTCGCGGGCGAAATCGCCGGGTCTGGTTTTG
GATCTGTGA

Upstream 100 bases:

>100_bases
CCATTTTCTTGCACGCCGGACAGTTCACCGCGAAGGGGAGTTATCTCTTCGCCGACCCTGGCCGCCCTGCAGACAGCGGA
GTGAGGGAGAGTAAACGGTA

Downstream 100 bases:

>100_bases
GGAGACTGACGATGACGCTCCCCTCCTCTTGCGCGCCGCTCACCGGTATCAGCCGCGCCCGACTCCCGGCCTGGGCGCTG
CCCGACGGCTGGCCGACGCG

Product: putative vanillate O-demethylase oxidoreductase

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MHSHDLISVSVGEIRPNGQGNLSIILQTAAGEILPAYSAGAHIDIIIPGVGPRQYSLCGTPDRSNTYEICVRLTDTSTGG
SRYLHQQLKAGDRLAISPPRNHFPLPQAGRYLLFAGGIGITPLLAMAEAIAARKGALELHYYVANSRQTAFSPRLNQLTA
NGTVAIHCSEEGASFRQRVPECLTVHHPDTVVIACGPEGFIQRLRSVMEEYRWSPSQFVFERFTPAAENNTAAKNAFYIE
LASSGQRLQVAADQTIAQVLQHAGVEVMLSCEQGMCGSCITGVLDGIPEHRDSVLTAEEKAGNDQITLCCSRAKSPGLVL
DL

Sequences:

>Translated_322_residues
MHSHDLISVSVGEIRPNGQGNLSIILQTAAGEILPAYSAGAHIDIIIPGVGPRQYSLCGTPDRSNTYEICVRLTDTSTGG
SRYLHQQLKAGDRLAISPPRNHFPLPQAGRYLLFAGGIGITPLLAMAEAIAARKGALELHYYVANSRQTAFSPRLNQLTA
NGTVAIHCSEEGASFRQRVPECLTVHHPDTVVIACGPEGFIQRLRSVMEEYRWSPSQFVFERFTPAAENNTAAKNAFYIE
LASSGQRLQVAADQTIAQVLQHAGVEVMLSCEQGMCGSCITGVLDGIPEHRDSVLTAEEKAGNDQITLCCSRAKSPGLVL
DL
>Mature_322_residues
MHSHDLISVSVGEIRPNGQGNLSIILQTAAGEILPAYSAGAHIDIIIPGVGPRQYSLCGTPDRSNTYEICVRLTDTSTGG
SRYLHQQLKAGDRLAISPPRNHFPLPQAGRYLLFAGGIGITPLLAMAEAIAARKGALELHYYVANSRQTAFSPRLNQLTA
NGTVAIHCSEEGASFRQRVPECLTVHHPDTVVIACGPEGFIQRLRSVMEEYRWSPSQFVFERFTPAAENNTAAKNAFYIE
LASSGQRLQVAADQTIAQVLQHAGVEVMLSCEQGMCGSCITGVLDGIPEHRDSVLTAEEKAGNDQITLCCSRAKSPGLVL
DL

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=330, Percent_Identity=37.5757575757576, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1787098, Length=242, Percent_Identity=25.6198347107438, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 34679; Mature: 34679

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSHDLISVSVGEIRPNGQGNLSIILQTAAGEILPAYSAGAHIDIIIPGVGPRQYSLCGT
CCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEECCCCCCEEEEEECCCCCCCEEECCC
PDRSNTYEICVRLTDTSTGGSRYLHQQLKAGDRLAISPPRNHFPLPQAGRYLLFAGGIGI
CCCCCCEEEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCCH
TPLLAMAEAIAARKGALELHYYVANSRQTAFSPRLNQLTANGTVAIHCSEEGASFRQRVP
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHEEECCCEEEEEECCCCCHHHHCCC
ECLTVHHPDTVVIACGPEGFIQRLRSVMEEYRWSPSQFVFERFTPAAENNTAAKNAFYIE
HHEEEECCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEE
LASSGQRLQVAADQTIAQVLQHAGVEVMLSCEQGMCGSCITGVLDGIPEHRDSVLTAEEK
ECCCCCEEEEEHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCHHCCEEEEHHH
AGNDQITLCCSRAKSPGLVLDL
CCCCEEEEEEECCCCCCEEEEC
>Mature Secondary Structure
MHSHDLISVSVGEIRPNGQGNLSIILQTAAGEILPAYSAGAHIDIIIPGVGPRQYSLCGT
CCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEECCCCCCEEEEEECCCCCCCEEECCC
PDRSNTYEICVRLTDTSTGGSRYLHQQLKAGDRLAISPPRNHFPLPQAGRYLLFAGGIGI
CCCCCCEEEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCCH
TPLLAMAEAIAARKGALELHYYVANSRQTAFSPRLNQLTANGTVAIHCSEEGASFRQRVP
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHEEECCCEEEEEECCCCCHHHHCCC
ECLTVHHPDTVVIACGPEGFIQRLRSVMEEYRWSPSQFVFERFTPAAENNTAAKNAFYIE
HHEEEECCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEE
LASSGQRLQVAADQTIAQVLQHAGVEVMLSCEQGMCGSCITGVLDGIPEHRDSVLTAEEK
ECCCCCEEEEEHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCHHCCEEEEHHH
AGNDQITLCCSRAKSPGLVLDL
CCCCEEEEEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3170489 [H]