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The map label for this gene is lcfB [H]

Identifier: 238894741

GI number: 238894741

Start: 2624077

End: 2625633

Strand: Reverse

Name: lcfB [H]

Synonym: KP1_2748

Alternate gene names: 238894741

Gene position: 2625633-2624077 (Counterclockwise)

Preceding gene: 238894742

Following gene: 238894740

Centisome position: 50.03

GC content: 59.47

Gene sequence:

>1557_bases
ATGAACAGCACCTCCGCCGACTTCCAGAATCTGTACCAGGCCCTGAGCCACAGCGCCGCCCGCAGCCCGGATGCCCTGGC
GCTGGCGTTTGAGGATCGGCGCTATCTGTACCGCGATTTTCATCTGCGGGTACAGCGGGCGATGGCGCAGCTTGATCGCG
GCTGGTCGCTGCGCAAAGGCGACCGTATTCTGCTGGCCTGGGGCAATCACCCCGCTTTTTGCGAAGTCCTTTTTGCCGCG
CTGGGTTTGGGCATCGAAGTGGTGCCTTTCAGCACCAAGCTTAAGCAGGCCGAAAGCGAGGCGCTGGTAGGCCATATCGC
TCCCCGGGTTGTCCTGTTCGATGCGACCGTACAGGACTGGCTGAAAAACACCCCTGACGCGCGTGCGGTTTCACTCACCG
AATGGCAGGCGCTCTGCCTTCCCGAACCGCTGACCCGCCCGCCGGTCCCGGTTAACCGCGACGATACGGCGGTGATGATG
TTCACCTCCGGCACCACCGGAGAGCCTAAAGGGGCGATTATCACCCACAATAACCTGCTCTGCGCGATTGACGCCTATAC
GCAAAAACTGAATCTCACCGCCGCGGATAGCACCATCCTGGCGGTGCCTATCTATCACATCACCGGCCTGTCGGCGCTGC
TGGCGCTGTTTATTAGCCTCGGCGCGAGCATCTGGCTGCAGCATCGGTTCAATGCCCCGCAGGTGATCACCACCCTGCGC
GAGCAGAACATTACCTTTCTGCACGGCTCGCCCACCATTTTTATCCTTCTTTGCCAGGCTGCGCGTGAGCAGAGCGCCAG
CCATCCCAGCGATTTTCCGGCATTGCGCACGATTGCCTGCGGCGCTGGCCACCTGAGCGATGGACTGATCAAGGAGTTAA
AAACCCTGTTCCCGCATACCGCGATTCAGCCAATCTACGGCCTCACCGAAACGACCTCGCCTGCGACGATTTTTCCGGGC
GACGTCTGGGGCAGCGATAAATGCGGCAGTTCAGGTCAGGCGATCCCGGGTCTCGCCATTACCATCCGCAACGATCGCCA
GCAGCCCCTCCCCGCCGGACAAATCGGCCATATCTGGCTAAAAGGCGATGTGGTGATCCGCGAATACTGGCAGCACAGCG
AGCGACGCCCGAGCTGCGATGCGCAGGGCTGGTTTTGTACCGGCGATCTGGGCTATCTCGACGACGAAGGCTGGCTGTAC
ATCAAAGACCGCAGCAAGGACATGATCAATCGCGGCGGCGAGAAAATTTACTCACTGGAGCTGGAAAACATTCTCTCTAC
CTATCGGGGAGTACGGGAAGTTGCCGTCATCCCGACCCCCAGCCCGGTCTACGGCGAAGAGCCCGTGGCGTTTATCGTGC
CGGATGGCCAGCATCACCTGACCAGCGAAGAGATCCTCGACTGGCTGAAGGTCAAAATCGCCCGTTTTAAGCTACCGGCG
CGCATTATTTTCACCCGTGCTTTACCGCGTACCCACAACGGCAAAGTGAGCAAACAGCAGCTCAAAACGCGTCTCGCAGA
ATCCATCATCACGCTATCAACGGAGGATAAAAAGTGA

Upstream 100 bases:

>100_bases
CTTCTGTTTTGTTGATTTTCTATTGTGAGCCATTCGGCAATTTCCTCTCCTGAATTTCCCCAGACTACTCCTACTCAATA
CGCGATATCAGGAGAGACAC

Downstream 100 bases:

>100_bases
GCAGCAAATTTATCGACGCCCATGAGGCGGCGCGGTGGGTCGCCAGCGGCGACACCGTCTGCACCGTCGGGATGACGCTT
ATCGGCGCGGCGGAGTCCAT

Product: putative acyl-CoA synthase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MNSTSADFQNLYQALSHSAARSPDALALAFEDRRYLYRDFHLRVQRAMAQLDRGWSLRKGDRILLAWGNHPAFCEVLFAA
LGLGIEVVPFSTKLKQAESEALVGHIAPRVVLFDATVQDWLKNTPDARAVSLTEWQALCLPEPLTRPPVPVNRDDTAVMM
FTSGTTGEPKGAIITHNNLLCAIDAYTQKLNLTAADSTILAVPIYHITGLSALLALFISLGASIWLQHRFNAPQVITTLR
EQNITFLHGSPTIFILLCQAAREQSASHPSDFPALRTIACGAGHLSDGLIKELKTLFPHTAIQPIYGLTETTSPATIFPG
DVWGSDKCGSSGQAIPGLAITIRNDRQQPLPAGQIGHIWLKGDVVIREYWQHSERRPSCDAQGWFCTGDLGYLDDEGWLY
IKDRSKDMINRGGEKIYSLELENILSTYRGVREVAVIPTPSPVYGEEPVAFIVPDGQHHLTSEEILDWLKVKIARFKLPA
RIIFTRALPRTHNGKVSKQQLKTRLAESIITLSTEDKK

Sequences:

>Translated_518_residues
MNSTSADFQNLYQALSHSAARSPDALALAFEDRRYLYRDFHLRVQRAMAQLDRGWSLRKGDRILLAWGNHPAFCEVLFAA
LGLGIEVVPFSTKLKQAESEALVGHIAPRVVLFDATVQDWLKNTPDARAVSLTEWQALCLPEPLTRPPVPVNRDDTAVMM
FTSGTTGEPKGAIITHNNLLCAIDAYTQKLNLTAADSTILAVPIYHITGLSALLALFISLGASIWLQHRFNAPQVITTLR
EQNITFLHGSPTIFILLCQAAREQSASHPSDFPALRTIACGAGHLSDGLIKELKTLFPHTAIQPIYGLTETTSPATIFPG
DVWGSDKCGSSGQAIPGLAITIRNDRQQPLPAGQIGHIWLKGDVVIREYWQHSERRPSCDAQGWFCTGDLGYLDDEGWLY
IKDRSKDMINRGGEKIYSLELENILSTYRGVREVAVIPTPSPVYGEEPVAFIVPDGQHHLTSEEILDWLKVKIARFKLPA
RIIFTRALPRTHNGKVSKQQLKTRLAESIITLSTEDKK
>Mature_518_residues
MNSTSADFQNLYQALSHSAARSPDALALAFEDRRYLYRDFHLRVQRAMAQLDRGWSLRKGDRILLAWGNHPAFCEVLFAA
LGLGIEVVPFSTKLKQAESEALVGHIAPRVVLFDATVQDWLKNTPDARAVSLTEWQALCLPEPLTRPPVPVNRDDTAVMM
FTSGTTGEPKGAIITHNNLLCAIDAYTQKLNLTAADSTILAVPIYHITGLSALLALFISLGASIWLQHRFNAPQVITTLR
EQNITFLHGSPTIFILLCQAAREQSASHPSDFPALRTIACGAGHLSDGLIKELKTLFPHTAIQPIYGLTETTSPATIFPG
DVWGSDKCGSSGQAIPGLAITIRNDRQQPLPAGQIGHIWLKGDVVIREYWQHSERRPSCDAQGWFCTGDLGYLDDEGWLY
IKDRSKDMINRGGEKIYSLELENILSTYRGVREVAVIPTPSPVYGEEPVAFIVPDGQHHLTSEEILDWLKVKIARFKLPA
RIIFTRALPRTHNGKVSKQQLKTRLAESIITLSTEDKK

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=552, Percent_Identity=27.1739130434783, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI187761345, Length=521, Percent_Identity=25.5278310940499, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI187761343, Length=521, Percent_Identity=25.5278310940499, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI42544132, Length=507, Percent_Identity=24.2603550295858, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI38505220, Length=503, Percent_Identity=24.6520874751491, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI62865631, Length=532, Percent_Identity=23.3082706766917, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI13325055, Length=532, Percent_Identity=23.3082706766917, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI115511026, Length=366, Percent_Identity=27.3224043715847, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI157311624, Length=495, Percent_Identity=23.8383838383838, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI157311622, Length=495, Percent_Identity=23.8383838383838, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI28416953, Length=586, Percent_Identity=23.8907849829352, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI58082049, Length=495, Percent_Identity=24.040404040404, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI13375727, Length=532, Percent_Identity=21.8045112781955, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI122937307, Length=513, Percent_Identity=22.4171539961014, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI227499619, Length=478, Percent_Identity=21.7573221757322, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI145693145, Length=354, Percent_Identity=32.4858757062147, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1788107, Length=543, Percent_Identity=27.0718232044199, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI221142682, Length=499, Percent_Identity=27.6553106212425, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1786810, Length=504, Percent_Identity=25.5952380952381, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1788595, Length=364, Percent_Identity=23.9010989010989, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789201, Length=371, Percent_Identity=24.2587601078167, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1786801, Length=457, Percent_Identity=22.3194748358862, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786529, Length=563, Percent_Identity=22.5577264653641, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17558820, Length=512, Percent_Identity=28.3203125, Blast_Score=198, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI32563687, Length=497, Percent_Identity=28.5714285714286, Blast_Score=186, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI71994690, Length=362, Percent_Identity=30.6629834254144, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI71994694, Length=362, Percent_Identity=30.6629834254144, Blast_Score=158, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI71994703, Length=362, Percent_Identity=30.6629834254144, Blast_Score=158, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17559526, Length=379, Percent_Identity=30.3430079155673, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17560308, Length=567, Percent_Identity=25.2204585537919, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17538037, Length=547, Percent_Identity=24.1316270566728, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17531443, Length=481, Percent_Identity=25.5717255717256, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71985884, Length=368, Percent_Identity=28.5326086956522, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71996755, Length=363, Percent_Identity=28.3746556473829, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=534, Percent_Identity=24.3445692883895, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17560140, Length=377, Percent_Identity=23.6074270557029, Blast_Score=125, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6319699, Length=358, Percent_Identity=27.9329608938547, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6323182, Length=586, Percent_Identity=22.3549488054608, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355181, Length=491, Percent_Identity=25.050916496945, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24581924, Length=352, Percent_Identity=28.6931818181818, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24648255, Length=388, Percent_Identity=25.7731958762887, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24648253, Length=385, Percent_Identity=25.974025974026, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI18859661, Length=373, Percent_Identity=24.3967828418231, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI19922652, Length=486, Percent_Identity=24.6913580246914, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21356947, Length=396, Percent_Identity=25.2525252525253, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21356441, Length=477, Percent_Identity=23.6897274633124, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24656500, Length=497, Percent_Identity=23.5412474849095, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281365686, Length=489, Percent_Identity=22.4948875255624, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI20130357, Length=419, Percent_Identity=24.3436754176611, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653035, Length=351, Percent_Identity=23.6467236467236, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21358303, Length=466, Percent_Identity=21.0300429184549, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648260, Length=355, Percent_Identity=25.0704225352113, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI161076582, Length=354, Percent_Identity=24.0112994350282, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24648257, Length=373, Percent_Identity=23.0563002680965, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24667955, Length=375, Percent_Identity=23.7333333333333, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI62472339, Length=375, Percent_Identity=23.7333333333333, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24667959, Length=372, Percent_Identity=23.6559139784946, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355225, Length=388, Percent_Identity=23.7113402061856, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 57426; Mature: 57426

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSTSADFQNLYQALSHSAARSPDALALAFEDRRYLYRDFHLRVQRAMAQLDRGWSLRKG
CCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
DRILLAWGNHPAFCEVLFAALGLGIEVVPFSTKLKQAESEALVGHIAPRVVLFDATVQDW
CEEEEEECCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEECHHHHH
LKNTPDARAVSLTEWQALCLPEPLTRPPVPVNRDDTAVMMFTSGTTGEPKGAIITHNNLL
HCCCCCCCEEEECCHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCEE
CAIDAYTQKLNLTAADSTILAVPIYHITGLSALLALFISLGASIWLQHRFNAPQVITTLR
EEEECHHHEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHCHHEEEEECCCCCHHHHHHH
EQNITFLHGSPTIFILLCQAAREQSASHPSDFPALRTIACGAGHLSDGLIKELKTLFPHT
HCCEEEEECCCEEEEEEEHHHHHCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCH
AIQPIYGLTETTSPATIFPGDVWGSDKCGSSGQAIPGLAITIRNDRQQPLPAGQIGHIWL
HHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEE
KGDVVIREYWQHSERRPSCDAQGWFCTGDLGYLDDEGWLYIKDRSKDMINRGGEKIYSLE
ECCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCHHHHHCCCCEEEEEE
LENILSTYRGVREVAVIPTPSPVYGEEPVAFIVPDGQHHLTSEEILDWLKVKIARFKLPA
HHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCH
RIIFTRALPRTHNGKVSKQQLKTRLAESIITLSTEDKK
HEEEEECCCCCCCCCCCHHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure
MNSTSADFQNLYQALSHSAARSPDALALAFEDRRYLYRDFHLRVQRAMAQLDRGWSLRKG
CCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
DRILLAWGNHPAFCEVLFAALGLGIEVVPFSTKLKQAESEALVGHIAPRVVLFDATVQDW
CEEEEEECCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHCCCEEEEEECHHHHH
LKNTPDARAVSLTEWQALCLPEPLTRPPVPVNRDDTAVMMFTSGTTGEPKGAIITHNNLL
HCCCCCCCEEEECCHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCCEE
CAIDAYTQKLNLTAADSTILAVPIYHITGLSALLALFISLGASIWLQHRFNAPQVITTLR
EEEECHHHEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHCHHEEEEECCCCCHHHHHHH
EQNITFLHGSPTIFILLCQAAREQSASHPSDFPALRTIACGAGHLSDGLIKELKTLFPHT
HCCEEEEECCCEEEEEEEHHHHHCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCH
AIQPIYGLTETTSPATIFPGDVWGSDKCGSSGQAIPGLAITIRNDRQQPLPAGQIGHIWL
HHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEEE
KGDVVIREYWQHSERRPSCDAQGWFCTGDLGYLDDEGWLYIKDRSKDMINRGGEKIYSLE
ECCCHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCHHHHHCCCCEEEEEE
LENILSTYRGVREVAVIPTPSPVYGEEPVAFIVPDGQHHLTSEEILDWLKVKIARFKLPA
HHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCH
RIIFTRALPRTHNGKVSKQQLKTRLAESIITLSTEDKK
HEEEEECCCCCCCCCCCHHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]