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The map label for this gene is yneJ [H]

Identifier: 238894669

GI number: 238894669

Start: 2558808

End: 2559686

Strand: Reverse

Name: yneJ [H]

Synonym: KP1_2674

Alternate gene names: 238894669

Gene position: 2559686-2558808 (Counterclockwise)

Preceding gene: 238894678

Following gene: 238894667

Centisome position: 48.77

GC content: 64.16

Gene sequence:

>879_bases
ATGGACCTGACTCAGCTAGAGATGTTTAACGCTGTCGCCAGCACCGGCAGCATTACCCAGGCGGCGCAGAAAGTGCATCG
CGTGCCGTCCAACCTCACCACCCGCATCCGCCAGCTGGAGGCCGACCTCGGCGTTGAGCTGTTTATCCGCGAGAACCAGC
GCCTGCGCCTGTCGCCTGCGGGACACAGCTTTTTGCGCTATAGCCAGCAGATCCTCGCCCTGGTGGACGAGGCGCGAATG
GTGGTGGCGGGCGACGAACCCCAGGGGCTGTTTTCTCTTGGTTCGCTGGAGAGCACCGCGGCGGTACGGATCCCCAGCAC
TCTGGCCCACTTTAATCAGCGCTACCCGAAAATTCATCTGGCGCTCTCAACCGGCCCTTCCGGGACGATGATCGACGGCG
TGCTGGAAGGCGCCCTGAGCGCCGCGTTTGTCGACGGCCCGCTGGTGCATCCGGGCCTGGAGGGGCTGCCGGTGTTCCCT
GAAGAGATGATGATCGTCGCCCCCTACGGCCACGCACCCATCACCCGCGCCAGCGAAGTGAACGGGGCCAACGTCTACGC
CTTTCGCGCCAACTGTTCCTATCGCCGGCATTTTGAGAGCTGGTTTCATGCCGACAGAGCGACCCCGGGCAGGATCCACG
AGATGGAGTCTTACCACGGGATGCTGGCCTGCGTCATCGCCGGCGCCGGCCTGGCGTTGATCCCGCGCAGCATGCTGGAG
AGTATGCCCGGCCATCAGCAGGTTTCCGCCTGGCCGCTGGCCGAAGAGTGGCGCTGGCTCACCACCTGGCTGGTGTGGCG
CCGGGGCGCCAAAACCCGCCAGCTGGAGGCGTTTATAGCGCTGCTGAACGAAGATCGGCAAACAGTGGTTTCTCCATAA

Upstream 100 bases:

>100_bases
ATTCTCTCTCGTTGCTTGCGAAGTGTCCGTATTCTGGCTTACCTTTCCATTCATTGAAAATGAATAATATTAAGCAACGC
ATTCACAAAAGAAGAATATC

Downstream 100 bases:

>100_bases
ACACGCTGAGCGGATCGGGTTGATACGGCGCAAACGCGCAGCGGGTCTGGTAGCCGTTGCGCGTGTAGAGGGCGATGGCC
GCATGCTGATGGATACCGGT

Product: putative LysR-family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MDLTQLEMFNAVASTGSITQAAQKVHRVPSNLTTRIRQLEADLGVELFIRENQRLRLSPAGHSFLRYSQQILALVDEARM
VVAGDEPQGLFSLGSLESTAAVRIPSTLAHFNQRYPKIHLALSTGPSGTMIDGVLEGALSAAFVDGPLVHPGLEGLPVFP
EEMMIVAPYGHAPITRASEVNGANVYAFRANCSYRRHFESWFHADRATPGRIHEMESYHGMLACVIAGAGLALIPRSMLE
SMPGHQQVSAWPLAEEWRWLTTWLVWRRGAKTRQLEAFIALLNEDRQTVVSP

Sequences:

>Translated_292_residues
MDLTQLEMFNAVASTGSITQAAQKVHRVPSNLTTRIRQLEADLGVELFIRENQRLRLSPAGHSFLRYSQQILALVDEARM
VVAGDEPQGLFSLGSLESTAAVRIPSTLAHFNQRYPKIHLALSTGPSGTMIDGVLEGALSAAFVDGPLVHPGLEGLPVFP
EEMMIVAPYGHAPITRASEVNGANVYAFRANCSYRRHFESWFHADRATPGRIHEMESYHGMLACVIAGAGLALIPRSMLE
SMPGHQQVSAWPLAEEWRWLTTWLVWRRGAKTRQLEAFIALLNEDRQTVVSP
>Mature_292_residues
MDLTQLEMFNAVASTGSITQAAQKVHRVPSNLTTRIRQLEADLGVELFIRENQRLRLSPAGHSFLRYSQQILALVDEARM
VVAGDEPQGLFSLGSLESTAAVRIPSTLAHFNQRYPKIHLALSTGPSGTMIDGVLEGALSAAFVDGPLVHPGLEGLPVFP
EEMMIVAPYGHAPITRASEVNGANVYAFRANCSYRRHFESWFHADRATPGRIHEMESYHGMLACVIAGAGLALIPRSMLE
SMPGHQQVSAWPLAEEWRWLTTWLVWRRGAKTRQLEAFIALLNEDRQTVVSP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=283, Percent_Identity=83.7455830388693, Blast_Score=503, Evalue=1e-144,
Organism=Escherichia coli, GI1788887, Length=302, Percent_Identity=25.1655629139073, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1790208, Length=256, Percent_Identity=26.953125, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082132, Length=288, Percent_Identity=23.6111111111111, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1787879, Length=278, Percent_Identity=26.6187050359712, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1790262, Length=288, Percent_Identity=24.6527777777778, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI157672245, Length=284, Percent_Identity=25, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788481, Length=179, Percent_Identity=26.2569832402235, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693105, Length=288, Percent_Identity=20.8333333333333, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1788297, Length=257, Percent_Identity=24.124513618677, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788706, Length=168, Percent_Identity=29.1666666666667, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786401, Length=165, Percent_Identity=26.6666666666667, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787601, Length=287, Percent_Identity=23.3449477351916, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32277; Mature: 32277

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTQLEMFNAVASTGSITQAAQKVHRVPSNLTTRIRQLEADLGVELFIRENQRLRLSPA
CCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCEEEECCC
GHSFLRYSQQILALVDEARMVVAGDEPQGLFSLGSLESTAAVRIPSTLAHFNQRYPKIHL
CHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEECCCCCCEEEECCHHHHHHHCCCCEEEE
ALSTGPSGTMIDGVLEGALSAAFVDGPLVHPGLEGLPVFPEEMMIVAPYGHAPITRASEV
EEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
NGANVYAFRANCSYRRHFESWFHADRATPGRIHEMESYHGMLACVIAGAGLALIPRSMLE
CCCEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SMPGHQQVSAWPLAEEWRWLTTWLVWRRGAKTRQLEAFIALLNEDRQTVVSP
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure
MDLTQLEMFNAVASTGSITQAAQKVHRVPSNLTTRIRQLEADLGVELFIRENQRLRLSPA
CCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCEEEECCC
GHSFLRYSQQILALVDEARMVVAGDEPQGLFSLGSLESTAAVRIPSTLAHFNQRYPKIHL
CHHHHHHHHHHHHHHHHCEEEEECCCCCCCEEECCCCCCEEEECCHHHHHHHCCCCEEEE
ALSTGPSGTMIDGVLEGALSAAFVDGPLVHPGLEGLPVFPEEMMIVAPYGHAPITRASEV
EEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
NGANVYAFRANCSYRRHFESWFHADRATPGRIHEMESYHGMLACVIAGAGLALIPRSMLE
CCCEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SMPGHQQVSAWPLAEEWRWLTTWLVWRRGAKTRQLEAFIALLNEDRQTVVSP
HCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]