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The map label for this gene is ogt [H]

Identifier: 238894549

GI number: 238894549

Start: 2445024

End: 2445539

Strand: Reverse

Name: ogt [H]

Synonym: KP1_2546

Alternate gene names: 238894549

Gene position: 2445539-2445024 (Counterclockwise)

Preceding gene: 238894551

Following gene: 238894548

Centisome position: 46.59

GC content: 62.02

Gene sequence:

>516_bases
ATGTTGACCTTGCTGCAGGATAAAATGGATACCCCGCTGGGGCCGTTGTGGGTGCTTTGCGACGAACAGTTTAATTTGCG
CGCCGTCGAGTGGGACGAGCACCGCGATCGGATGGAAACGCTGCTCGACGTACACTATCGTCGGGAAGGCTATCAGCGCG
TCGACTGCCGCAATCCCGGCGGTCTCAGCAGTAAGCTGAGCGACTATTTTGCCGGCGATTTAGCCATTATCGAGACTCTG
CCTACCGCCACCGCCGGCACCCCTTTTCAGCGCCAGGTCTGGCAGGCGTTACGCGATATCCCCTGTGGCCAGGTGATGCA
CTACGGCCAACTGGCCGAGGCGCTCGGTCGTCCCGGCGCCGCGCGCGCGGTGGGCGCCGCCAATGGCGCCAATCCGGTGA
GTATCGTGGTGCCCTGCCATCGGGTCATCGGGCGCAACGGCACCATGACCGGCTATGCCGGCGGCGTACAGCGTAAAGAG
TGGCTTTTGCGTCATGAGGGCTATCTGTTGCTTTGA

Upstream 100 bases:

>100_bases
GCTATCTGGCGATGCGGTTTTACCTTTTGGGAGTATAGTTGACTGCCGTCAGCGCGTGGTATCGTGTGCCTCTCATGTAC
GATGGTGGATGACAGAAACG

Downstream 100 bases:

>100_bases
CTGAAAATGCCTTTTCTGGAATTTTCAGGTAATTTTACAATCAAAAGTGGCGTAATAATCTGTTTTTAATCAAATAGATA
CACAGTCGATTTGATTTACC

Product: O-6-alkylguanine-DNA:cysteine-protein methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MLTLLQDKMDTPLGPLWVLCDEQFNLRAVEWDEHRDRMETLLDVHYRREGYQRVDCRNPGGLSSKLSDYFAGDLAIIETL
PTATAGTPFQRQVWQALRDIPCGQVMHYGQLAEALGRPGAARAVGAANGANPVSIVVPCHRVIGRNGTMTGYAGGVQRKE
WLLRHEGYLLL

Sequences:

>Translated_171_residues
MLTLLQDKMDTPLGPLWVLCDEQFNLRAVEWDEHRDRMETLLDVHYRREGYQRVDCRNPGGLSSKLSDYFAGDLAIIETL
PTATAGTPFQRQVWQALRDIPCGQVMHYGQLAEALGRPGAARAVGAANGANPVSIVVPCHRVIGRNGTMTGYAGGVQRKE
WLLRHEGYLLL
>Mature_171_residues
MLTLLQDKMDTPLGPLWVLCDEQFNLRAVEWDEHRDRMETLLDVHYRREGYQRVDCRNPGGLSSKLSDYFAGDLAIIETL
PTATAGTPFQRQVWQALRDIPCGQVMHYGQLAEALGRPGAARAVGAANGANPVSIVVPCHRVIGRNGTMTGYAGGVQRKE
WLLRHEGYLLL

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=112, Percent_Identity=45.5357142857143, Blast_Score=87, Evalue=6e-18,
Organism=Escherichia coli, GI1787596, Length=171, Percent_Identity=80.1169590643275, Blast_Score=299, Evalue=6e-83,
Organism=Escherichia coli, GI1788542, Length=89, Percent_Identity=56.1797752808989, Blast_Score=109, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI115533068, Length=81, Percent_Identity=43.2098765432099, Blast_Score=76, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI115533070, Length=81, Percent_Identity=43.2098765432099, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=46.9135802469136, Blast_Score=89, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17137554, Length=97, Percent_Identity=42.2680412371134, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19059; Mature: 19059

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLLQDKMDTPLGPLWVLCDEQFNLRAVEWDEHRDRMETLLDVHYRREGYQRVDCRNPG
CCCCCCHHCCCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHCCCEECCCCCCC
GLSSKLSDYFAGDLAIIETLPTATAGTPFQRQVWQALRDIPCGQVMHYGQLAEALGRPGA
CCHHHHHHHHCCCHHEEHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
ARAVGAANGANPVSIVVPCHRVIGRNGTMTGYAGGVQRKEWLLRHEGYLLL
CCCCCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEC
>Mature Secondary Structure
MLTLLQDKMDTPLGPLWVLCDEQFNLRAVEWDEHRDRMETLLDVHYRREGYQRVDCRNPG
CCCCCCHHCCCCCCCEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHCCCEECCCCCCC
GLSSKLSDYFAGDLAIIETLPTATAGTPFQRQVWQALRDIPCGQVMHYGQLAEALGRPGA
CCHHHHHHHHCCCHHEEHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
ARAVGAANGANPVSIVVPCHRVIGRNGTMTGYAGGVQRKEWLLRHEGYLLL
CCCCCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]