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The map label for this gene is paaJ [H]

Identifier: 238894490

GI number: 238894490

Start: 2383968

End: 2385173

Strand: Direct

Name: paaJ [H]

Synonym: KP1_2481

Alternate gene names: 238894490

Gene position: 2383968-2385173 (Clockwise)

Preceding gene: 238894489

Following gene: 238894491

Centisome position: 45.42

GC content: 64.68

Gene sequence:

>1206_bases
ATGCGCGACGCCTTTATTTGTGACGGGATCCGTACGCCGATTGGCCGCTACGGCGGCGCGCTGGCCAGTGTGCGCGCCGA
CGACCTGGCGGCGATCCCGCTGCGTGAGCTCCTGAGTCGTAATCCCGGGCTCGACCCGGCAGCAATTGATGATGTGATTT
TCGGCTGTGCGAATCAGGCCGGGGAAGATAACCGCAACGTGGCGCACATGGCGACGCTGTTGGCCGGTTACCCGCATACC
GTGCCGGGCACCACGATTAACCGCCTGTGCGGATCGGGTCTGGATGCCATCGGCTTTGCCGCCAGGGCGATCAAAGCCGG
CGATGCCGATCTGCTGATTGCCGGCGGCGTGGAATCGATGTCGCGTGCGCCTTTCGTGATGGGAAAAGCCAGCGCGCCGT
ACCAGCGTCAGGCGGAACTCTTTGATACCACGATTGGCTGGCGTTTTGTGAACCCGCTCATGGCGCAACACTTCGGAACT
GACAGCATGCCGGAAACGGCCGAGAATGTAGCTGAATTGTTAAATATCAGCCGGGCCGATCAGGACGCTTTCGCCTGGCG
CAGCCAGCAGCGCGCGGCGCAGGCCCAGCGCGACGGCATTCTGGCGCAGGAGATTGTGCCGGTGCAGATTGTCGGCCGGA
AAGGGGCGGTTAGCGACGTGCGCGAGGACGAGCATCCGCGCCCGGAAACCACGCTTGAGCAGCTGGCGAAACTGAAAGCG
CCGTTCCGCCAGGGCGGCGTCATTACTGCGGGCAATGCTTCAGGCGTCAACGATGGCGCCGCGGCGCTGATCATCGCCAG
CGAACAGCAGGCCGCTATCCAGGGGCTCACGCCGCGGGCGCGTATCGTGGCGATGGCGACGGCGGGTGTCGAACCGCGCC
TGATGGGACTGGGGCCGGTACCGGCGGTGCGTAAAGTGCTGGAGCGGGCGGGACTCAATATTAACGATATGGATCTGATT
GAGCTGAATGAGGCCTTCGCCGCCCAGGCGCTGGGGGTGCTCAGACAGCTCGGCGTGCCGGACGATGCGGCGCACGTGAA
TCCCAACGGCGGCGCCATCGCGTTAGGCCATCCGCTGGGCATGAGCGGCGCGCGGTTAGCGCTGAGCGCCAGCCTTGAGC
TGCAACGCCGGGGCGGGCGCTATGCGCTGTGTACGATGTGTATTGGCGTGGGTCAGGGCATTGCCATGATCCTCGAGCGA
GTGTGA

Upstream 100 bases:

>100_bases
GCGTCTACGACATCGAAATTGTTAATCAACAGCAAAAGATTGTGGCCCTGTTCCGCGGCAAATCACACCGCATCGGCGGC
ACAATCACGGGAGACGTGTA

Downstream 100 bases:

>100_bases
GCTAATTTACCTGCGAGTACCCTACAATGATAACTACTACAAAATTAGATCCGATTGAAACCGCATCGCGTGACGAACTG
CAGGCCCTGCAAACCCAGCG

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MRDAFICDGIRTPIGRYGGALASVRADDLAAIPLRELLSRNPGLDPAAIDDVIFGCANQAGEDNRNVAHMATLLAGYPHT
VPGTTINRLCGSGLDAIGFAARAIKAGDADLLIAGGVESMSRAPFVMGKASAPYQRQAELFDTTIGWRFVNPLMAQHFGT
DSMPETAENVAELLNISRADQDAFAWRSQQRAAQAQRDGILAQEIVPVQIVGRKGAVSDVREDEHPRPETTLEQLAKLKA
PFRQGGVITAGNASGVNDGAAALIIASEQQAAIQGLTPRARIVAMATAGVEPRLMGLGPVPAVRKVLERAGLNINDMDLI
ELNEAFAAQALGVLRQLGVPDDAAHVNPNGGAIALGHPLGMSGARLALSASLELQRRGGRYALCTMCIGVGQGIAMILER
V

Sequences:

>Translated_401_residues
MRDAFICDGIRTPIGRYGGALASVRADDLAAIPLRELLSRNPGLDPAAIDDVIFGCANQAGEDNRNVAHMATLLAGYPHT
VPGTTINRLCGSGLDAIGFAARAIKAGDADLLIAGGVESMSRAPFVMGKASAPYQRQAELFDTTIGWRFVNPLMAQHFGT
DSMPETAENVAELLNISRADQDAFAWRSQQRAAQAQRDGILAQEIVPVQIVGRKGAVSDVREDEHPRPETTLEQLAKLKA
PFRQGGVITAGNASGVNDGAAALIIASEQQAAIQGLTPRARIVAMATAGVEPRLMGLGPVPAVRKVLERAGLNINDMDLI
ELNEAFAAQALGVLRQLGVPDDAAHVNPNGGAIALGHPLGMSGARLALSASLELQRRGGRYALCTMCIGVGQGIAMILER
V
>Mature_401_residues
MRDAFICDGIRTPIGRYGGALASVRADDLAAIPLRELLSRNPGLDPAAIDDVIFGCANQAGEDNRNVAHMATLLAGYPHT
VPGTTINRLCGSGLDAIGFAARAIKAGDADLLIAGGVESMSRAPFVMGKASAPYQRQAELFDTTIGWRFVNPLMAQHFGT
DSMPETAENVAELLNISRADQDAFAWRSQQRAAQAQRDGILAQEIVPVQIVGRKGAVSDVREDEHPRPETTLEQLAKLKA
PFRQGGVITAGNASGVNDGAAALIIASEQQAAIQGLTPRARIVAMATAGVEPRLMGLGPVPAVRKVLERAGLNINDMDLI
ELNEAFAAQALGVLRQLGVPDDAAHVNPNGGAIALGHPLGMSGARLALSASLELQRRGGRYALCTMCIGVGQGIAMILER
V

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=399, Percent_Identity=43.1077694235589, Blast_Score=298, Evalue=4e-81,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=40.0503778337531, Blast_Score=278, Evalue=5e-75,
Organism=Homo sapiens, GI4501853, Length=407, Percent_Identity=40.04914004914, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI4557237, Length=402, Percent_Identity=36.0696517412935, Blast_Score=232, Evalue=6e-61,
Organism=Homo sapiens, GI4504327, Length=431, Percent_Identity=33.1786542923434, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI194353979, Length=399, Percent_Identity=29.8245614035088, Blast_Score=141, Evalue=1e-33,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=84.788029925187, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI48994986, Length=404, Percent_Identity=44.5544554455446, Blast_Score=307, Evalue=9e-85,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=45.409429280397, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=44.139650872818, Blast_Score=284, Evalue=6e-78,
Organism=Escherichia coli, GI1788683, Length=424, Percent_Identity=33.4905660377358, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI133906874, Length=404, Percent_Identity=42.8217821782178, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI25147385, Length=402, Percent_Identity=36.0696517412935, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17535921, Length=408, Percent_Identity=33.8235294117647, Blast_Score=206, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17551802, Length=434, Percent_Identity=30.6451612903226, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17535917, Length=407, Percent_Identity=30.2211302211302, Blast_Score=164, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6325229, Length=411, Percent_Identity=35.5231143552311, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6322031, Length=414, Percent_Identity=36.9565217391304, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=45.3865336658354, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI17648125, Length=406, Percent_Identity=40.1477832512315, Blast_Score=265, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24640423, Length=404, Percent_Identity=34.1584158415842, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17137578, Length=431, Percent_Identity=31.322505800464, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 42112; Mature: 42112

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDAFICDGIRTPIGRYGGALASVRADDLAAIPLRELLSRNPGLDPAAIDDVIFGCANQA
CCCCEEECCHHCCHHHHCCHHHHCCCCCHHHCCHHHHHHCCCCCCHHHHHHHHHHHHCCC
GEDNRNVAHMATLLAGYPHTVPGTTINRLCGSGLDAIGFAARAIKAGDADLLIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCHHHH
SRAPFVMGKASAPYQRQAELFDTTIGWRFVNPLMAQHFGTDSMPETAENVAELLNISRAD
HCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
QDAFAWRSQQRAAQAQRDGILAQEIVPVQIVGRKGAVSDVREDEHPRPETTLEQLAKLKA
HHHHHHHHHHHHHHHHHCCCHHHHCCEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHC
PFRQGGVITAGNASGVNDGAAALIIASEQQAAIQGLTPRARIVAMATAGVEPRLMGLGPV
HHHCCCEEEECCCCCCCCCCEEEEEECCHHHHHHCCCCCCEEEEEEECCCCCEEECCCCC
PAVRKVLERAGLNINDMDLIELNEAFAAQALGVLRQLGVPDDAAHVNPNGGAIALGHPLG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCC
MSGARLALSASLELQRRGGRYALCTMCIGVGQGIAMILERV
CCCCEEEEEECHHHHHCCCCEEHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MRDAFICDGIRTPIGRYGGALASVRADDLAAIPLRELLSRNPGLDPAAIDDVIFGCANQA
CCCCEEECCHHCCHHHHCCHHHHCCCCCHHHCCHHHHHHCCCCCCHHHHHHHHHHHHCCC
GEDNRNVAHMATLLAGYPHTVPGTTINRLCGSGLDAIGFAARAIKAGDADLLIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCHHHH
SRAPFVMGKASAPYQRQAELFDTTIGWRFVNPLMAQHFGTDSMPETAENVAELLNISRAD
HCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCC
QDAFAWRSQQRAAQAQRDGILAQEIVPVQIVGRKGAVSDVREDEHPRPETTLEQLAKLKA
HHHHHHHHHHHHHHHHHCCCHHHHCCEEEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHC
PFRQGGVITAGNASGVNDGAAALIIASEQQAAIQGLTPRARIVAMATAGVEPRLMGLGPV
HHHCCCEEEECCCCCCCCCCEEEEEECCHHHHHHCCCCCCEEEEEEECCCCCEEECCCCC
PAVRKVLERAGLNINDMDLIELNEAFAAQALGVLRQLGVPDDAAHVNPNGGAIALGHPLG
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEECCCCC
MSGARLALSASLELQRRGGRYALCTMCIGVGQGIAMILERV
CCCCEEEEEECHHHHHCCCCEEHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 10766858 [H]