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The map label for this gene is nadE [H]

Identifier: 238894283

GI number: 238894283

Start: 2173708

End: 2174535

Strand: Reverse

Name: nadE [H]

Synonym: KP1_2260

Alternate gene names: 238894283

Gene position: 2174535-2173708 (Counterclockwise)

Preceding gene: 238894291

Following gene: 238894282

Centisome position: 41.43

GC content: 56.16

Gene sequence:

>828_bases
ATGACGCTACAACAAGAGATTATTCAGGCGCTGGGCGCTAAGCCGCAGATTGATGTCGCAGGCGAGATCCGCCGCAGCGT
CGATTTCCTGAAGTCATACCTGCAGACCTACCCGTTTATTAAATCGCTGGTGCTGGGTATCAGCGGCGGTCAGGACTCCA
CCCTGACGGGAAAACTGTGCCAGATAGCGATTAACGAACTGCGTGCCGAAACCGGCGACAGTAGCCTGCAGTTTATCGCC
GTCCGTTTACCCTATGGCGTGCAGGCTGATGAGCAGGACTGCCAGGACGCCATCGCATTTATCCAGCCTGACCGGGTGTT
GACAGTCAATATTAAAGCTGCGGTGCTGGCCAGCGAACAGGCGCTGCGCGAAGCAGGCATCGAACTGAGCGACTTCGTTC
GCGGAAATGAAAAAGCCCGTGAGCGAATGAAGGCCCAGTACAGCATCGCCGGAATGACCAAAGGAGTTGTGGTCGGTACC
GACCATGCGGCGGAAGCGATTACCGGCTTTTTCACCAAATATGGCGACGGCGGCACCGACATCAACCCAATTTTCCGCCT
GAACAAACGTCAGGGCAAACAGCTGCTGGCCCATCTCGGCTGCCCGGAACATCTGTATAAGAAGCTGCCCACCGCCGACC
TGGAAGACGATCGCCCCTCCCTGCCGGATGAAGTGGCCCTGGGTGTCACCTACGAGAACATCGACGATTATCTGGAAGGC
AAAACGCTGGACCCGAGCATTGCCAAAACGATCGAGGGCTGGTATCTGAAAACCGAACACAAGCGTCGGCCGCCGATCAC
CGTCTTTGACGATTTCTGGAAAAAATAG

Upstream 100 bases:

>100_bases
TCTTTTCCGGCAGTCAAAACCCCGGTCCTTGTCGTTTCAGCGGCAAAGAGGTAGCTTTAAGCCAACAGCTTGCCAAACAG
ATTCACTTTCGGAGGGCGCG

Downstream 100 bases:

>100_bases
TCTCTCCCCCCGCCCCGGCCTGCGCCGGGGTTATTTTTTGTCCTTTTCGCCGCAAAAATGGCCTGCTATACTGGATAGAT
AGACAGTACCAGGAGTTATT

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTLQQEIIQALGAKPQIDVAGEIRRSVDFLKSYLQTYPFIKSLVLGISGGQDSTLTGKLCQIAINELRAETGDSSLQFIA
VRLPYGVQADEQDCQDAIAFIQPDRVLTVNIKAAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTKGVVVGT
DHAAEAITGFFTKYGDGGTDINPIFRLNKRQGKQLLAHLGCPEHLYKKLPTADLEDDRPSLPDEVALGVTYENIDDYLEG
KTLDPSIAKTIEGWYLKTEHKRRPPITVFDDFWKK

Sequences:

>Translated_275_residues
MTLQQEIIQALGAKPQIDVAGEIRRSVDFLKSYLQTYPFIKSLVLGISGGQDSTLTGKLCQIAINELRAETGDSSLQFIA
VRLPYGVQADEQDCQDAIAFIQPDRVLTVNIKAAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTKGVVVGT
DHAAEAITGFFTKYGDGGTDINPIFRLNKRQGKQLLAHLGCPEHLYKKLPTADLEDDRPSLPDEVALGVTYENIDDYLEG
KTLDPSIAKTIEGWYLKTEHKRRPPITVFDDFWKK
>Mature_274_residues
TLQQEIIQALGAKPQIDVAGEIRRSVDFLKSYLQTYPFIKSLVLGISGGQDSTLTGKLCQIAINELRAETGDSSLQFIAV
RLPYGVQADEQDCQDAIAFIQPDRVLTVNIKAAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTKGVVVGTD
HAAEAITGFFTKYGDGGTDINPIFRLNKRQGKQLLAHLGCPEHLYKKLPTADLEDDRPSLPDEVALGVTYENIDDYLEGK
TLDPSIAKTIEGWYLKTEHKRRPPITVFDDFWKK

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=275, Percent_Identity=90.1818181818182, Blast_Score=517, Evalue=1e-148,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 30489; Mature: 30358

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00548 RIBOSOMAL_S3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQQEIIQALGAKPQIDVAGEIRRSVDFLKSYLQTYPFIKSLVLGISGGQDSTLTGKLC
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
QIAINELRAETGDSSLQFIAVRLPYGVQADEQDCQDAIAFIQPDRVLTVNIKAAVLASEQ
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHH
ALREAGIELSDFVRGNEKARERMKAQYSIAGMTKGVVVGTDHAAEAITGFFTKYGDGGTD
HHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCC
INPIFRLNKRQGKQLLAHLGCPEHLYKKLPTADLEDDRPSLPDEVALGVTYENIDDYLEG
CCHHHEECHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHEECEEHHHHHHHHCC
KTLDPSIAKTIEGWYLKTEHKRRPPITVFDDFWKK
CCCCHHHHHHHHCCEEECCCCCCCCCEEHHHHHCC
>Mature Secondary Structure 
TLQQEIIQALGAKPQIDVAGEIRRSVDFLKSYLQTYPFIKSLVLGISGGQDSTLTGKLC
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
QIAINELRAETGDSSLQFIAVRLPYGVQADEQDCQDAIAFIQPDRVLTVNIKAAVLASEQ
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHH
ALREAGIELSDFVRGNEKARERMKAQYSIAGMTKGVVVGTDHAAEAITGFFTKYGDGGTD
HHHHHCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCC
INPIFRLNKRQGKQLLAHLGCPEHLYKKLPTADLEDDRPSLPDEVALGVTYENIDDYLEG
CCHHHEECHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHEECEEHHHHHHHHCC
KTLDPSIAKTIEGWYLKTEHKRRPPITVFDDFWKK
CCCCHHHHHHHHCCEEECCCCCCCCCEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA