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The map label for this gene is htrB [H]

Identifier: 238894104

GI number: 238894104

Start: 2005043

End: 2005963

Strand: Reverse

Name: htrB [H]

Synonym: KP1_2055

Alternate gene names: 238894104

Gene position: 2005963-2005043 (Counterclockwise)

Preceding gene: 238894106

Following gene: 238894103

Centisome position: 38.22

GC content: 59.28

Gene sequence:

>921_bases
ATGACTCATTTGCCTAAATTTTCTCCGGCGCTGTTGCATCCGCGCTATTGGCTGCTGTGGCTGGGTATCGGCCTGCTGTG
GCTGGTTGTCCAACTGCCCTACCCGCTGATCTACCGCCTCGGGAACGCCATCGGCCGGCTGGCCATGCGCTTTATGAAGC
GTCGGGCGAAGATCGCCTATCGCAACCTTGAGCTCTGCTTCCCCGACAAAAGCGAGCAGGAACGTCACCGAATGGTGGTG
ATGAACTTTGAATCGGTGGGTATGGGACTGATGGAGACGGGGATGGCCTGGTTCTGGCCGGTTCGTCGTATCGCCCGCTG
GACCGACACCATCGGCTTTGAGCATATCCGCGATGTGCAGGCGCAGCAGCGCGGCATTCTGCTGATCGGTATTCATTTCC
TCACTCTCGAGATGGGTGCGCGGATGTTCGGTATGAACGAGCCGGGGATTGGCGTCTATCGCCCCAACGACAACCCGGTG
ATCGACTGGCTGCAGACCTGGGGCCGCTTGCGCTCCAATAAGGATATGATTGACCGTAAGGATCTGAAAGGCATGATCCG
GGCACTGAAAAAAGGCGAAGTGGTGTGGTACGCACCGGATCACGATTACGGCCCGACCGCCAGCGTCTTTGCCCCACTGT
TTGCGGTCGACCAGGCGGCGACCACCTCCGGCACCTGGATGCTGGCGAAAATGTCCAAAGCCTGCATCGTGCCCTTCGTC
CCGCGCCGCAAACCGGACGGTAAAGGTTATGAACTGATTATCCTGCCGCCAGAGTGTTCCCCGCCGCTGGACGACGCGGA
AACCACCGCCGCGTGGATGAACAAGATCGTTGAGCAGTGCATCATGATGGCCCCGGAACAGTATATGTGGCTCCATCGTC
GCTTCAAAACCCGCCCCGAAGGCATGCCCTCCCGCTACTGA

Upstream 100 bases:

>100_bases
TCGCGCCCCTCTTAACAAAACGAGCTGACTGTCGCCGCCTTTGGCTGGCCGGTAAAAAAATGCGATAATATAAGACGTTG
CTCACTGATACGGGTATGAA

Downstream 100 bases:

>100_bases
TCCCTTTTCCCGGCCGCGCTCATCCCGGCCGGGAAGCTTCGCCGCTCAGGCTTTAGGCTCTCCTTTAGCTTTAAAAGCTC
TCCTCCATTGCGGCATCTAT

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MTHLPKFSPALLHPRYWLLWLGIGLLWLVVQLPYPLIYRLGNAIGRLAMRFMKRRAKIAYRNLELCFPDKSEQERHRMVV
MNFESVGMGLMETGMAWFWPVRRIARWTDTIGFEHIRDVQAQQRGILLIGIHFLTLEMGARMFGMNEPGIGVYRPNDNPV
IDWLQTWGRLRSNKDMIDRKDLKGMIRALKKGEVVWYAPDHDYGPTASVFAPLFAVDQAATTSGTWMLAKMSKACIVPFV
PRRKPDGKGYELIILPPECSPPLDDAETTAAWMNKIVEQCIMMAPEQYMWLHRRFKTRPEGMPSRY

Sequences:

>Translated_306_residues
MTHLPKFSPALLHPRYWLLWLGIGLLWLVVQLPYPLIYRLGNAIGRLAMRFMKRRAKIAYRNLELCFPDKSEQERHRMVV
MNFESVGMGLMETGMAWFWPVRRIARWTDTIGFEHIRDVQAQQRGILLIGIHFLTLEMGARMFGMNEPGIGVYRPNDNPV
IDWLQTWGRLRSNKDMIDRKDLKGMIRALKKGEVVWYAPDHDYGPTASVFAPLFAVDQAATTSGTWMLAKMSKACIVPFV
PRRKPDGKGYELIILPPECSPPLDDAETTAAWMNKIVEQCIMMAPEQYMWLHRRFKTRPEGMPSRY
>Mature_305_residues
THLPKFSPALLHPRYWLLWLGIGLLWLVVQLPYPLIYRLGNAIGRLAMRFMKRRAKIAYRNLELCFPDKSEQERHRMVVM
NFESVGMGLMETGMAWFWPVRRIARWTDTIGFEHIRDVQAQQRGILLIGIHFLTLEMGARMFGMNEPGIGVYRPNDNPVI
DWLQTWGRLRSNKDMIDRKDLKGMIRALKKGEVVWYAPDHDYGPTASVFAPLFAVDQAATTSGTWMLAKMSKACIVPFVP
RRKPDGKGYELIILPPECSPPLDDAETTAAWMNKIVEQCIMMAPEQYMWLHRRFKTRPEGMPSRY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=306, Percent_Identity=85.9477124183007, Blast_Score=563, Evalue=1e-162,
Organism=Escherichia coli, GI87082094, Length=301, Percent_Identity=52.8239202657807, Blast_Score=347, Evalue=5e-97,
Organism=Escherichia coli, GI1788161, Length=310, Percent_Identity=30.6451612903226, Blast_Score=124, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35526; Mature: 35395

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
6.2 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHLPKFSPALLHPRYWLLWLGIGLLWLVVQLPYPLIYRLGNAIGRLAMRFMKRRAKIAY
CCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLELCFPDKSEQERHRMVVMNFESVGMGLMETGMAWFWPVRRIARWTDTIGFEHIRDVQ
HCCEEECCCCCCCCCCCEEEEEHHHHCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
AQQRGILLIGIHFLTLEMGARMFGMNEPGIGVYRPNDNPVIDWLQTWGRLRSNKDMIDRK
HHHCCEEEEHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCHHHHH
DLKGMIRALKKGEVVWYAPDHDYGPTASVFAPLFAVDQAATTSGTWMLAKMSKACIVPFV
HHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCC
PRRKPDGKGYELIILPPECSPPLDDAETTAAWMNKIVEQCIMMAPEQYMWLHRRFKTRPE
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GMPSRY
CCCCCC
>Mature Secondary Structure 
THLPKFSPALLHPRYWLLWLGIGLLWLVVQLPYPLIYRLGNAIGRLAMRFMKRRAKIAY
CCCCCCCHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLELCFPDKSEQERHRMVVMNFESVGMGLMETGMAWFWPVRRIARWTDTIGFEHIRDVQ
HCCEEECCCCCCCCCCCEEEEEHHHHCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
AQQRGILLIGIHFLTLEMGARMFGMNEPGIGVYRPNDNPVIDWLQTWGRLRSNKDMIDRK
HHHCCEEEEHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCHHHHH
DLKGMIRALKKGEVVWYAPDHDYGPTASVFAPLFAVDQAATTSGTWMLAKMSKACIVPFV
HHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCC
PRRKPDGKGYELIILPPECSPPLDDAETTAAWMNKIVEQCIMMAPEQYMWLHRRFKTRPE
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GMPSRY
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]