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The map label for this gene is puuB [H]

Identifier: 238894053

GI number: 238894053

Start: 1954308

End: 1955588

Strand: Reverse

Name: puuB [H]

Synonym: KP1_2001

Alternate gene names: 238894053

Gene position: 1955588-1954308 (Counterclockwise)

Preceding gene: 238894054

Following gene: 238894051

Centisome position: 37.26

GC content: 62.69

Gene sequence:

>1281_bases
ATGACTGAACATACCACTAGCTACTATGCCGCCAGCGCCAACAAGTATGAACCGTTCCCGACGCTGGAGGGATCCATCAA
CTGTGACGTCTGCGTCGTCGGCGGCGGCTACACCGGCCTCTCGTCTGCCCTGCATCTGGCCGAAATGGGCTACGACGTGG
TGGTGCTGGAAGGGGCGCGCATCGGCTTCGGCGCCAGCGGCCGCAACGGCGGTCAGCTGGTCAACTCCTATAGCCGCGAT
ATCGACGTCATTGAAAAGAACTACGGCCCGGATGCGGCAAAAATGCTCGGCAGCATGATGTTCGAAGGCGGCGATATCAT
CCGCGAACGCATCCAGCGGTATCAGATCCAGTGCGATTACCGCCCGGGCGGCCTGTTCGTGGCGATGAACCATAAACAGC
TGGAAACCCTGGAAGAGCAGAAAGCCAACTGGGAGCGCTACGGCAACACCCAGCTGGAGCTGCTGGACAGAGAGGCCATT
CGTCGCGAAGTGGACAGCGACCGCTACGTCGGCGCCCTGCTCGACCACAGCGGCGGCCATATTCACCCGCTGAACCTTGC
CATCGGCGAAGCGGATGCCATTCGCCTGAACGGCGGCCGGGTGTATGAGCAGTCGCCGGTCACCCGCATCCAGCACACCA
GCCCGGCGGTGGTCAGCACCGCGCGCGGCCAGGTCACCGCGCGCTACGTGATCGTCGCCGGCAACGCCTATCTCGGCGAT
AAACTGGAGCCGGAGCTGGCGAAACGCAGCATGCCGTGCGGCACCCAGGTGGTCACTACCGCTCCGCTGTCGGAAGAGGT
CGCCCGCTCGCTGATCCCGAAAAACTACTGTGTGGAAGACTGTAACTACCTGCTGGACTACTACCGTCTGACCGGCGACA
ACCGTCTGCTGTACGGCGGCGGTGTAGTCTACGGCGCGCGCGATCCGGACGACGTGGAGCGGCTGATTATGCCGAAACTG
CTGAAAACCTTCCCGCAGCTGCAGGGGGTGAAGATTGACTACCGCTGGACGGGCAACTTCCTGCTAACCCTGTCGCGGAT
GCCGCAGTTCGGGCGTCTCGATAACAATATTTACTACATGCAGGGCTACAGCGGCCACGGCGTGACCTGTACCCACCTTG
CCGGACGCTTGATTTCAGAGCTGCTGCGCGGCGACGCCGAGCGTTTCGACGCCTTCGCCAAACTGCCGCACTACCCGTTC
CCGGGCGGCCGCAGCCTGCGCATTCCTTTTACCGCCATGGGCGCCGCCTACTACAGCCTGCGCGACCGCCTTGGGGTTTA
A

Upstream 100 bases:

>100_bases
GCGGCTACAAGCAGAGCGGCAACGGGCGCGATAAATCGCTGCACGCACTGGAAAAATTCACCGAACTGAAAACCATCTGG
ATTGCCCTGGAGTCTTGATC

Downstream 100 bases:

>100_bases
TCGCCTCTGCCGACGGCGCTGGCCGTCGGCTCATGCTGCGGATAGTGCTGCTGAAAGTAGTGGCGCAGAAACTCCACGGT
GGTGCGGATTTTAGCCGACG

Product: putative oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTEHTTSYYAASANKYEPFPTLEGSINCDVCVVGGGYTGLSSALHLAEMGYDVVVLEGARIGFGASGRNGGQLVNSYSRD
IDVIEKNYGPDAAKMLGSMMFEGGDIIRERIQRYQIQCDYRPGGLFVAMNHKQLETLEEQKANWERYGNTQLELLDREAI
RREVDSDRYVGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYEQSPVTRIQHTSPAVVSTARGQVTARYVIVAGNAYLGD
KLEPELAKRSMPCGTQVVTTAPLSEEVARSLIPKNYCVEDCNYLLDYYRLTGDNRLLYGGGVVYGARDPDDVERLIMPKL
LKTFPQLQGVKIDYRWTGNFLLTLSRMPQFGRLDNNIYYMQGYSGHGVTCTHLAGRLISELLRGDAERFDAFAKLPHYPF
PGGRSLRIPFTAMGAAYYSLRDRLGV

Sequences:

>Translated_426_residues
MTEHTTSYYAASANKYEPFPTLEGSINCDVCVVGGGYTGLSSALHLAEMGYDVVVLEGARIGFGASGRNGGQLVNSYSRD
IDVIEKNYGPDAAKMLGSMMFEGGDIIRERIQRYQIQCDYRPGGLFVAMNHKQLETLEEQKANWERYGNTQLELLDREAI
RREVDSDRYVGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYEQSPVTRIQHTSPAVVSTARGQVTARYVIVAGNAYLGD
KLEPELAKRSMPCGTQVVTTAPLSEEVARSLIPKNYCVEDCNYLLDYYRLTGDNRLLYGGGVVYGARDPDDVERLIMPKL
LKTFPQLQGVKIDYRWTGNFLLTLSRMPQFGRLDNNIYYMQGYSGHGVTCTHLAGRLISELLRGDAERFDAFAKLPHYPF
PGGRSLRIPFTAMGAAYYSLRDRLGV
>Mature_425_residues
TEHTTSYYAASANKYEPFPTLEGSINCDVCVVGGGYTGLSSALHLAEMGYDVVVLEGARIGFGASGRNGGQLVNSYSRDI
DVIEKNYGPDAAKMLGSMMFEGGDIIRERIQRYQIQCDYRPGGLFVAMNHKQLETLEEQKANWERYGNTQLELLDREAIR
REVDSDRYVGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYEQSPVTRIQHTSPAVVSTARGQVTARYVIVAGNAYLGDK
LEPELAKRSMPCGTQVVTTAPLSEEVARSLIPKNYCVEDCNYLLDYYRLTGDNRLLYGGGVVYGARDPDDVERLIMPKLL
KTFPQLQGVKIDYRWTGNFLLTLSRMPQFGRLDNNIYYMQGYSGHGVTCTHLAGRLISELLRGDAERFDAFAKLPHYPFP
GGRSLRIPFTAMGAAYYSLRDRLGV

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=426, Percent_Identity=88.9671361502347, Blast_Score=793, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47297; Mature: 47166

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHTTSYYAASANKYEPFPTLEGSINCDVCVVGGGYTGLSSALHLAEMGYDVVVLEGAR
CCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECCE
IGFGASGRNGGQLVNSYSRDIDVIEKNYGPDAAKMLGSMMFEGGDIIRERIQRYQIQCDY
EECCCCCCCCHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHEEEEEEE
RPGGLFVAMNHKQLETLEEQKANWERYGNTQLELLDREAIRREVDSDRYVGALLDHSGGH
CCCEEEEEECCHHHHHHHHHHCCHHHCCCCCEEHHHHHHHHHHCCCCCEEEEEEECCCCE
IHPLNLAIGEADAIRLNGGRVYEQSPVTRIQHTSPAVVSTARGQVTARYVIVAGNAYLGD
EEEEEEEECCCCEEEECCCEEECCCCCCEEECCCCCEEEECCCCEEEEEEEEECCCCCCC
KLEPELAKRSMPCGTQVVTTAPLSEEVARSLIPKNYCVEDCNYLLDYYRLTGDNRLLYGG
CCCHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHEECCCCEEEEEC
GVVYGARDPDDVERLIMPKLLKTFPQLQGVKIDYRWTGNFLLTLSRMPQFGRLDNNIYYM
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCEEEE
QGYSGHGVTCTHLAGRLISELLRGDAERFDAFAKLPHYPFPGGRSLRIPFTAMGAAYYSL
ECCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHH
RDRLGV
HHHHCC
>Mature Secondary Structure 
TEHTTSYYAASANKYEPFPTLEGSINCDVCVVGGGYTGLSSALHLAEMGYDVVVLEGAR
CCCCCCEEEECCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEEECCE
IGFGASGRNGGQLVNSYSRDIDVIEKNYGPDAAKMLGSMMFEGGDIIRERIQRYQIQCDY
EECCCCCCCCHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHEEEEEEE
RPGGLFVAMNHKQLETLEEQKANWERYGNTQLELLDREAIRREVDSDRYVGALLDHSGGH
CCCEEEEEECCHHHHHHHHHHCCHHHCCCCCEEHHHHHHHHHHCCCCCEEEEEEECCCCE
IHPLNLAIGEADAIRLNGGRVYEQSPVTRIQHTSPAVVSTARGQVTARYVIVAGNAYLGD
EEEEEEEECCCCEEEECCCEEECCCCCCEEECCCCCEEEECCCCEEEEEEEEECCCCCCC
KLEPELAKRSMPCGTQVVTTAPLSEEVARSLIPKNYCVEDCNYLLDYYRLTGDNRLLYGG
CCCHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHEECCCCEEEEEC
GVVYGARDPDDVERLIMPKLLKTFPQLQGVKIDYRWTGNFLLTLSRMPQFGRLDNNIYYM
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCEEEE
QGYSGHGVTCTHLAGRLISELLRGDAERFDAFAKLPHYPFPGGRSLRIPFTAMGAAYYSL
ECCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHH
RDRLGV
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]