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The map label for this gene is dmlA [H]

Identifier: 238894051

GI number: 238894051

Start: 1952181

End: 1953266

Strand: Reverse

Name: dmlA [H]

Synonym: KP1_1998

Alternate gene names: 238894051

Gene position: 1953266-1952181 (Counterclockwise)

Preceding gene: 238894053

Following gene: 238894050

Centisome position: 37.22

GC content: 58.93

Gene sequence:

>1086_bases
ATGAAAAAAACCTGTCGTATTGCCGCCATCCCGGGTGACGGAATTGGCAAAGAAGTGCTGCCTGAAGGGATTCGCGTGCT
GCAAGCCGCCGAACAGCGTTGGGATCTGTCGCTGAGCTTCGAGCAGATGGAGTGGGCAAGCTGCGAATATTACGCCCATC
ATGGCAAGATGATGCCGGACGACTGGCGCGAGCAGCTGCAGGGCTTCGATGCTATCTACTTCGGCGCCGTCGGCTGGCCG
GATACCGTTCCCGACCACATTTCGCTGTGGGGGTCGCTGCTGAAGTTCCGCCGCGAGTTCGATCAATACGTCAACCTGCG
CCCGGTGCGCCTGTTCCCCGGCGTCCCCTGCCCGCTGGCGGGTAAAAAGGCCGGCGATATTGATTTTTACGTGGTGCGCG
AAAATACCGAAGGCGAATATTCCGCCCTTGGCGGCCGCGCCAATGAAGGCACCGAGCATGAATTGGTGATTCAGGAGTCG
GTCTTCACCCGCCGCGGCGTTAACCGGATCCTGCGCTACGCCTTTGAGCTGGCGCAAAGCCGCCCACGCAAAACGCTGAC
CTCCGCAACCAAATCCAATGGCCTGGCCATCAGCATGCCCTATTGGGATGAGCGGGTGGAAGAGATGGCCAAAAACTATC
CCGAAATTCGCTGGGACAAGCAGCATATCGATATCCTGTGTGCCCGTTTCGTGCTGCAGCCGGAGCGTTTCGACGTGGTA
GTGGGTTCTAACCTGTTCGGCGATATTCTCTCCGATCTCGGCCCGGCCTGTACCGGCACTATCGGTATTGCGCCATCGGC
CAATCTCAATCCGGAGCGCAATTTCCCGTCGCTGTTCGAACCGGTTCACGGGTCGGCGCCGGATATTTACGGTAAAAATA
TCGCCAACCCGATCGCCACCGTTTGGGCCGGGGCGATGATGCTCGATTTCCTCGGCAACGGCGATGAGCGCTACCACGCC
GCTCACGATGGCATTCTGGCGGCGATTGAGCAGACGATCGCCTGCGGACCGAAAACTCCGGACATGAAAGGCAGTGCCTC
AACGCAGCAGGTTAGCGAGGCTATCTGCAAGGCCATTTTGGCGTAA

Upstream 100 bases:

>100_bases
CTGTGTTTTATGTTTCACCAGTTAGCGTGATTAATTATGAACCAGTCAATTATAGGATAGGCAGGTGGTCAAACATCATC
GTTAAAAAGTGAGAACCGGA

Downstream 100 bases:

>100_bases
TTACCCCGCCGCTCCCCGGTGGCGCTGCGCTTACCGGGGCTACAATCGCACGATAATGTAGTGTGTAGGCCGGGTAAGGC
ACAGCCGCCACCCGGCAAAA

Product: putative tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKKTCRIAAIPGDGIGKEVLPEGIRVLQAAEQRWDLSLSFEQMEWASCEYYAHHGKMMPDDWREQLQGFDAIYFGAVGWP
DTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLAGKKAGDIDFYVVRENTEGEYSALGGRANEGTEHELVIQES
VFTRRGVNRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEEMAKNYPEIRWDKQHIDILCARFVLQPERFDVV
VGSNLFGDILSDLGPACTGTIGIAPSANLNPERNFPSLFEPVHGSAPDIYGKNIANPIATVWAGAMMLDFLGNGDERYHA
AHDGILAAIEQTIACGPKTPDMKGSASTQQVSEAICKAILA

Sequences:

>Translated_361_residues
MKKTCRIAAIPGDGIGKEVLPEGIRVLQAAEQRWDLSLSFEQMEWASCEYYAHHGKMMPDDWREQLQGFDAIYFGAVGWP
DTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLAGKKAGDIDFYVVRENTEGEYSALGGRANEGTEHELVIQES
VFTRRGVNRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEEMAKNYPEIRWDKQHIDILCARFVLQPERFDVV
VGSNLFGDILSDLGPACTGTIGIAPSANLNPERNFPSLFEPVHGSAPDIYGKNIANPIATVWAGAMMLDFLGNGDERYHA
AHDGILAAIEQTIACGPKTPDMKGSASTQQVSEAICKAILA
>Mature_361_residues
MKKTCRIAAIPGDGIGKEVLPEGIRVLQAAEQRWDLSLSFEQMEWASCEYYAHHGKMMPDDWREQLQGFDAIYFGAVGWP
DTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLAGKKAGDIDFYVVRENTEGEYSALGGRANEGTEHELVIQES
VFTRRGVNRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEEMAKNYPEIRWDKQHIDILCARFVLQPERFDVV
VGSNLFGDILSDLGPACTGTIGIAPSANLNPERNFPSLFEPVHGSAPDIYGKNIANPIATVWAGAMMLDFLGNGDERYHA
AHDGILAAIEQTIACGPKTPDMKGSASTQQVSEAICKAILA

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=362, Percent_Identity=32.8729281767956, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI4758582, Length=363, Percent_Identity=30.0275482093664, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI28178816, Length=355, Percent_Identity=26.1971830985915, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI28178838, Length=310, Percent_Identity=30.9677419354839, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI28178821, Length=351, Percent_Identity=25.6410256410256, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI28178819, Length=197, Percent_Identity=28.9340101522843, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1788101, Length=360, Percent_Identity=90, Blast_Score=684, Evalue=0.0,
Organism=Escherichia coli, GI87081683, Length=369, Percent_Identity=37.6693766937669, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1787381, Length=380, Percent_Identity=26.8421052631579, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71986051, Length=365, Percent_Identity=31.5068493150685, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17550882, Length=361, Percent_Identity=27.9778393351801, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17505779, Length=361, Percent_Identity=29.6398891966759, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=364, Percent_Identity=28.5714285714286, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6322097, Length=372, Percent_Identity=38.7096774193548, Blast_Score=213, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6319830, Length=374, Percent_Identity=34.7593582887701, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=365, Percent_Identity=32.6027397260274, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324291, Length=365, Percent_Identity=30.1369863013699, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24643268, Length=364, Percent_Identity=32.6923076923077, Blast_Score=168, Evalue=6e-42,
Organism=Drosophila melanogaster, GI24643270, Length=364, Percent_Identity=32.6923076923077, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24661184, Length=364, Percent_Identity=31.5934065934066, Blast_Score=157, Evalue=7e-39,
Organism=Drosophila melanogaster, GI161078635, Length=275, Percent_Identity=34.5454545454545, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI161078639, Length=275, Percent_Identity=34.5454545454545, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI161078637, Length=275, Percent_Identity=34.5454545454545, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI161078633, Length=275, Percent_Identity=34.5454545454545, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24650122, Length=275, Percent_Identity=34.5454545454545, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI281362242, Length=365, Percent_Identity=29.3150684931507, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24648872, Length=365, Percent_Identity=29.3150684931507, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI20130355, Length=366, Percent_Identity=24.0437158469945, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 40122; Mature: 40122

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTCRIAAIPGDGIGKEVLPEGIRVLQAAEQRWDLSLSFEQMEWASCEYYAHHGKMMPD
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCH
DWREQLQGFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLA
HHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
GKKAGDIDFYVVRENTEGEYSALGGRANEGTEHELVIQESVFTRRGVNRILRYAFELAQS
CCCCCCEEEEEEECCCCCCHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHC
RPRKTLTSATKSNGLAISMPYWDERVEEMAKNYPEIRWDKQHIDILCARFVLQPERFDVV
CCHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCEECHHHHHHHHHHHHCCCCCEEEE
VGSNLFGDILSDLGPACTGTIGIAPSANLNPERNFPSLFEPVHGSAPDIYGKNIANPIAT
ECCHHHHHHHHHCCCHHCCCEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHH
VWAGAMMLDFLGNGDERYHAAHDGILAAIEQTIACGPKTPDMKGSASTQQVSEAICKAIL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHC
A
C
>Mature Secondary Structure
MKKTCRIAAIPGDGIGKEVLPEGIRVLQAAEQRWDLSLSFEQMEWASCEYYAHHGKMMPD
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCH
DWREQLQGFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLA
HHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
GKKAGDIDFYVVRENTEGEYSALGGRANEGTEHELVIQESVFTRRGVNRILRYAFELAQS
CCCCCCEEEEEEECCCCCCHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHC
RPRKTLTSATKSNGLAISMPYWDERVEEMAKNYPEIRWDKQHIDILCARFVLQPERFDVV
CCHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCEECHHHHHHHHHHHHCCCCCEEEE
VGSNLFGDILSDLGPACTGTIGIAPSANLNPERNFPSLFEPVHGSAPDIYGKNIANPIAT
ECCHHHHHHHHHCCCHHCCCEECCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHH
VWAGAMMLDFLGNGDERYHAAHDGILAAIEQTIACGPKTPDMKGSASTQQVSEAICKAIL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]