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The map label for this gene is dmlR [H]

Identifier: 238894052

GI number: 238894052

Start: 1953349

End: 1954311

Strand: Direct

Name: dmlR [H]

Synonym: KP1_2000

Alternate gene names: 238894052

Gene position: 1953349-1954311 (Clockwise)

Preceding gene: 238894046

Following gene: 238894058

Centisome position: 37.22

GC content: 60.85

Gene sequence:

>963_bases
GTGAAACATAAAACACAGATCATGAATAATCTGCCGCTGCTAAATGATTTACGCGTCTTTATGCTGGTCGCTCGCCGGGC
CGGGTTCGCCGCCGCCGCCGAGGAGCTTGGCGTATCGCCGGCGTTCGTCAGCAAGCGGGTTTCGCTGCTGGAGCAAACCC
TGAATGTGATGTTGCTGCACCGGACCACCCGTCGGGTCACCATTACCGAAGAGGGGGAGCGGATCTATGAATGGGCGCAG
CGCATTTTGCAGGATGTCGATGAGATGATGGATGAGCTTTCCGACGTGCGCCAGGTGCCGCAGGGAACGCTGCGTATTAT
CAGTAGCTTCGGCTTTGGCCGTCGGGTTGTCGCCCCGGCGCTGTCGGCGCTGGCTCTCCAGTACCCGCAGCTGGAGCTGC
GTTTCGATGTCCAGGACCGGCTGGTGGATTTAGTCAATGAAGGGGTGGATCTCGATATTCGCGTCGGCGACGATATCGCA
CCGAATCTGATTGCCCGCCAGCTGGCGGCCAATCACCGCGTACTCTGCGCCTCGCCGCAGTTCCTTGCCCGCCATGCGCC
GCCGAAACAGCTGAGCGATCTGGCCGCGTTACCCTGCCTGGTGATTAAAGAGCGCGATCACCCGTTCGGCGTCTGGCAAT
TGCATAGCAAAGAGGGCCAGCACGCCATCAAGGTAACCGGGCCGCTCTCCTCCAACCATGGTGAAATTGTGCATCAGTGG
TGTCTGGACGGGCAGGGGATTGCGCTCCGTTCGTGGTGGGACGTCCGGGAGAATATCGCCAGCGGACATCTGGTGCAGGT
GCTGCCGGACTACTGGCAGCCGGCCAACGTCTGGGCGGTTTACGTCTCCCGGCTGGCGACGTCGGCTAAAATCCGCACCA
CCGTGGAGTTTCTGCGCCACTACTTTCAGCAGCACTATCCGCAGCATGAGCCGACGGCCAGCGCCGTCGGCAGAGGCGAT
TAA

Upstream 100 bases:

>100_bases
ACGACAGGTTTTTTTCATTCCGGTTCTCACTTTTTAACGATGATGTTTGACCACCTGCCTATCCTATAATTGACTGGTTC
ATAATTAATCACGCTAACTG

Downstream 100 bases:

>100_bases
ACCCCAAGGCGGTCGCGCAGGCTGTAGTAGGCGGCGCCCATGGCGGTAAAAGGAATGCGCAGGCTGCGGCCGCCCGGGAA
CGGGTAGTGCGGCAGTTTGG

Product: putative LysR-family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKHKTQIMNNLPLLNDLRVFMLVARRAGFAAAAEELGVSPAFVSKRVSLLEQTLNVMLLHRTTRRVTITEEGERIYEWAQ
RILQDVDEMMDELSDVRQVPQGTLRIISSFGFGRRVVAPALSALALQYPQLELRFDVQDRLVDLVNEGVDLDIRVGDDIA
PNLIARQLAANHRVLCASPQFLARHAPPKQLSDLAALPCLVIKERDHPFGVWQLHSKEGQHAIKVTGPLSSNHGEIVHQW
CLDGQGIALRSWWDVRENIASGHLVQVLPDYWQPANVWAVYVSRLATSAKIRTTVEFLRHYFQQHYPQHEPTASAVGRGD

Sequences:

>Translated_320_residues
MKHKTQIMNNLPLLNDLRVFMLVARRAGFAAAAEELGVSPAFVSKRVSLLEQTLNVMLLHRTTRRVTITEEGERIYEWAQ
RILQDVDEMMDELSDVRQVPQGTLRIISSFGFGRRVVAPALSALALQYPQLELRFDVQDRLVDLVNEGVDLDIRVGDDIA
PNLIARQLAANHRVLCASPQFLARHAPPKQLSDLAALPCLVIKERDHPFGVWQLHSKEGQHAIKVTGPLSSNHGEIVHQW
CLDGQGIALRSWWDVRENIASGHLVQVLPDYWQPANVWAVYVSRLATSAKIRTTVEFLRHYFQQHYPQHEPTASAVGRGD
>Mature_320_residues
MKHKTQIMNNLPLLNDLRVFMLVARRAGFAAAAEELGVSPAFVSKRVSLLEQTLNVMLLHRTTRRVTITEEGERIYEWAQ
RILQDVDEMMDELSDVRQVPQGTLRIISSFGFGRRVVAPALSALALQYPQLELRFDVQDRLVDLVNEGVDLDIRVGDDIA
PNLIARQLAANHRVLCASPQFLARHAPPKQLSDLAALPCLVIKERDHPFGVWQLHSKEGQHAIKVTGPLSSNHGEIVHQW
CLDGQGIALRSWWDVRENIASGHLVQVLPDYWQPANVWAVYVSRLATSAKIRTTVEFLRHYFQQHYPQHEPTASAVGRGD

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=301, Percent_Identity=87.7076411960133, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI1789440, Length=298, Percent_Identity=46.6442953020134, Blast_Score=287, Evalue=8e-79,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=34.3642611683849, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787128, Length=303, Percent_Identity=29.3729372937294, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=30.4347826086957, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789639, Length=291, Percent_Identity=30.5841924398625, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787589, Length=269, Percent_Identity=27.1375464684015, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789173, Length=270, Percent_Identity=31.4814814814815, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788706, Length=265, Percent_Identity=29.4339622641509, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786448, Length=257, Percent_Identity=29.9610894941634, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786508, Length=268, Percent_Identity=25, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI157672245, Length=149, Percent_Identity=28.8590604026846, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786713, Length=146, Percent_Identity=34.2465753424658, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36158; Mature: 36158

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHKTQIMNNLPLLNDLRVFMLVARRAGFAAAAEELGVSPAFVSKRVSLLEQTLNVMLLH
CCCHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RTTRRVTITEEGERIYEWAQRILQDVDEMMDELSDVRQVPQGTLRIISSFGFGRRVVAPA
HCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHH
LSALALQYPQLELRFDVQDRLVDLVNEGVDLDIRVGDDIAPNLIARQLAANHRVLCASPQ
HHHHHHHCCCEEEEECHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEECCHH
FLARHAPPKQLSDLAALPCLVIKERDHPFGVWQLHSKEGQHAIKVTGPLSSNHGEIVHQW
HHHHCCCHHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHH
CLDGQGIALRSWWDVRENIASGHLVQVLPDYWQPANVWAVYVSRLATSAKIRTTVEFLRH
HCCCCCEEEHHHHHHHHHHCCCCHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YFQQHYPQHEPTASAVGRGD
HHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKHKTQIMNNLPLLNDLRVFMLVARRAGFAAAAEELGVSPAFVSKRVSLLEQTLNVMLLH
CCCHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RTTRRVTITEEGERIYEWAQRILQDVDEMMDELSDVRQVPQGTLRIISSFGFGRRVVAPA
HCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHH
LSALALQYPQLELRFDVQDRLVDLVNEGVDLDIRVGDDIAPNLIARQLAANHRVLCASPQ
HHHHHHHCCCEEEEECHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEECCHH
FLARHAPPKQLSDLAALPCLVIKERDHPFGVWQLHSKEGQHAIKVTGPLSSNHGEIVHQW
HHHHCCCHHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHH
CLDGQGIALRSWWDVRENIASGHLVQVLPDYWQPANVWAVYVSRLATSAKIRTTVEFLRH
HCCCCCEEEHHHHHHHHHHCCCCHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YFQQHYPQHEPTASAVGRGD
HHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]