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The map label for this gene is pflD [H]

Identifier: 238894043

GI number: 238894043

Start: 1939854

End: 1942151

Strand: Reverse

Name: pflD [H]

Synonym: KP1_1989

Alternate gene names: 238894043

Gene position: 1942151-1939854 (Counterclockwise)

Preceding gene: 238894044

Following gene: 238894042

Centisome position: 37.0

GC content: 58.44

Gene sequence:

>2298_bases
ATGACGAATCGTACCCAACGCCTGAAAGCCAGCCTGTTCGCCCAGCCGCGCGAAATCTCCCTTGAGCGTGCATTACTCTA
CACTGCCAGCCATCGGCAGACGGAGGGCGAGCCGGTGATTATCCGCCGGGCGAAAGCCACCGCGTGGATCCTCGATAAGG
TGATGATTTCGATTCGTGAGGATGAACTGATCGCCGGCAACCGCACCGTCAAACCGCGGGCAGGGATTATGTCGCCCGAG
ATGGACCCCTACTGGCTGCTCAACGAGCTCGACGCCTTCCCGACCCGCCCACAGGACCGCTTCGCGATAAGCGAAGAAGA
TAAGCAAATCTACCGCGAAACGCTGTACCCCTACTGGGAAAAGCGTTCAATGAAAGATTTCATCAACGGCCAGATGACCG
AAGAGGTCAAGGCCGCCGTCAACACCCAGATATTTAGCGTGAACCAGACCGATAAAGGCCAGGGCCATATCATCATCGAC
TATCCACGGCTGCTGAATCATGGCCTTGGCGCCCTGGTCTCCGAGCTCAAAACGCACTGCGCCCGGCAACCGGAGAACCC
CTTTTATCAGGCGGTACTGATCCTGCTGGAAGCTTCCCAGCGCCATATTCTGCGCTACGCCGCGCTGGCCGAGGAGATGG
CAGGGCACTGTCAGGACCCGCAGCGTCAGCAGGAGCTGTTAACCATCGCCGCTATTTCGCGCCACAACGCGCAGCATCAG
CCAACGGACTTCCCCCAGGCCTGCCAGCTGTTCTGGTATATGAATATCATTCTGCAATACGAATCCAACGCCAGCTCGAT
TTCACTCGGACGCTTTGACCAGTACATGCTGCCGTTTTATCAGGCATCGCTGAATCAGGGTCAGGATCCCGCATATCTCA
AGGAGTTGCTGGAGTCGCTCTGGGTGAAATGCAACGATATCGTCCTGCTACGCTCCTCCAGCAGCGCGCGCTATTTCGCG
GGTTTTCCCACCGGCTATACCGCCCTGCTCGGCGGGCTGACGGACACCGGGCGCAGCGCGGTCAACGTGCTGTCATTTTT
GTGCCTCGACGCCTATCAAAACGTTCAGCTGCCGCAGCCGAATCTGGGCGTGCGGGTCAACGAGCTGATTGATCGCCCGT
TCCTGCGCAAAACTGCCGAAACCATCCGCCTGGGCACCGGCATCCCGCAGATTTTCAACGATGAGGTGGTCGTGCCGGCC
TTCCTTAACCGCGGCGTGTCGCTGGAAGATGCCAGAGATTACGCGGTGGTCGGCTGCGTGGAGCTCTCTATTCCAGGGCG
CACCTACGGCCTGCATGATATCGCCATGTTTAATCTGCTGAAGGTGATGGAAATTGTGCTTCAGGAAAATGAACATCAGC
CTGACGTCAGCTGGGACGGCCTTATCTGCCAGATCCGCGACAAAATCCGCCATTACATCAAGCTGATGGTCGAGGGCAGC
AACATTTGCGATATCGGCCACCGCGACTGGGCGCCGGTCCCGCTGCTCTCCTCGTTCATTGAAGATTGCGTGCAGCACGG
CAAAGATATCACCGCCGGCGGCGCGCGCTATAACTTCTCCGGCGTTCAGGGTATCGGAATCGCTAACCTGAGCGATTCGC
TGTACGCCCTGAAGGGTATGGTCTTCGACCAGCAGCGTCTCAGCTTTGACCAACTGATGGCCGTGCTGAAAGCCAATTTC
CAGACGCCGGAAGGGAAAAAGATCCGCGCCCGGCTGATTAACCGTTTTGACAAGTACGGCAACGATATCGACGATGTCGA
CAATATTAGCGCCGATCTGCTGCGTTTTTACTGCAAAGAAGTCGAGCAGTATCTGAACCCGCGCGGCGGTCATTTTACCC
CCGGCTCCTACACCGTCTCCGCGCACGTGCCGCTGGGTTCGGTGGTGGGCGCCACCCCGGACGGGCGGCTGGCCGGAGAA
CAGCTGGCGGACGGCGGCCTGTCGCCGATGGTTGGTCAGGATTCCCAGGGGCCGACGGCAGTGCTGAAATCGGTCAGCAA
GCTGGATAACTATCTGCTGTCGAACGGTACGCTGCTGAACGTCAAATTCACTCCGGCAACGCTGGCCGGCGACGGCGGCC
TGAACAAACTGGCCGATTTTTTACAGGCCTTCTGCAAGCTCAAGCTGCAGCATATTCAGTTCAACGTGGTCAATGCCGAC
ACCCTGCGTGAAGCGCAGCAGCGGCCGCAGGACTTTGCCGGTCTGGTGGTACGGGTCGCGGGCTACAGCGCCTTCTTTGT
TGAGCTGTCGAAGGAGATTCAGGATGACATTATCCGCCGCACCGCGCATCAGCTGTGA

Upstream 100 bases:

>100_bases
GCCGACGCCATCATGAACAAGATAGACAGCCACCTTTCCCAGAGCGCCTGAAGCCTTAAAAGGGCGCCTTCGCGCCCTTC
GCCACAACATGGAGTTTATC

Downstream 100 bases:

>100_bases
GGTCATCGACACGCGCGCCGACCGGGCGCGTATTTTTAACCTCCAGCGCTACTCGCTCAATGACGGACAAGGGATCCGCA
CGGTGGTCTTTTTTAAAGGC

Product: putative formate acetyltransferase 2

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 765; Mature: 764

Protein sequence:

>765_residues
MTNRTQRLKASLFAQPREISLERALLYTASHRQTEGEPVIIRRAKATAWILDKVMISIREDELIAGNRTVKPRAGIMSPE
MDPYWLLNELDAFPTRPQDRFAISEEDKQIYRETLYPYWEKRSMKDFINGQMTEEVKAAVNTQIFSVNQTDKGQGHIIID
YPRLLNHGLGALVSELKTHCARQPENPFYQAVLILLEASQRHILRYAALAEEMAGHCQDPQRQQELLTIAAISRHNAQHQ
PTDFPQACQLFWYMNIILQYESNASSISLGRFDQYMLPFYQASLNQGQDPAYLKELLESLWVKCNDIVLLRSSSSARYFA
GFPTGYTALLGGLTDTGRSAVNVLSFLCLDAYQNVQLPQPNLGVRVNELIDRPFLRKTAETIRLGTGIPQIFNDEVVVPA
FLNRGVSLEDARDYAVVGCVELSIPGRTYGLHDIAMFNLLKVMEIVLQENEHQPDVSWDGLICQIRDKIRHYIKLMVEGS
NICDIGHRDWAPVPLLSSFIEDCVQHGKDITAGGARYNFSGVQGIGIANLSDSLYALKGMVFDQQRLSFDQLMAVLKANF
QTPEGKKIRARLINRFDKYGNDIDDVDNISADLLRFYCKEVEQYLNPRGGHFTPGSYTVSAHVPLGSVVGATPDGRLAGE
QLADGGLSPMVGQDSQGPTAVLKSVSKLDNYLLSNGTLLNVKFTPATLAGDGGLNKLADFLQAFCKLKLQHIQFNVVNAD
TLREAQQRPQDFAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL

Sequences:

>Translated_765_residues
MTNRTQRLKASLFAQPREISLERALLYTASHRQTEGEPVIIRRAKATAWILDKVMISIREDELIAGNRTVKPRAGIMSPE
MDPYWLLNELDAFPTRPQDRFAISEEDKQIYRETLYPYWEKRSMKDFINGQMTEEVKAAVNTQIFSVNQTDKGQGHIIID
YPRLLNHGLGALVSELKTHCARQPENPFYQAVLILLEASQRHILRYAALAEEMAGHCQDPQRQQELLTIAAISRHNAQHQ
PTDFPQACQLFWYMNIILQYESNASSISLGRFDQYMLPFYQASLNQGQDPAYLKELLESLWVKCNDIVLLRSSSSARYFA
GFPTGYTALLGGLTDTGRSAVNVLSFLCLDAYQNVQLPQPNLGVRVNELIDRPFLRKTAETIRLGTGIPQIFNDEVVVPA
FLNRGVSLEDARDYAVVGCVELSIPGRTYGLHDIAMFNLLKVMEIVLQENEHQPDVSWDGLICQIRDKIRHYIKLMVEGS
NICDIGHRDWAPVPLLSSFIEDCVQHGKDITAGGARYNFSGVQGIGIANLSDSLYALKGMVFDQQRLSFDQLMAVLKANF
QTPEGKKIRARLINRFDKYGNDIDDVDNISADLLRFYCKEVEQYLNPRGGHFTPGSYTVSAHVPLGSVVGATPDGRLAGE
QLADGGLSPMVGQDSQGPTAVLKSVSKLDNYLLSNGTLLNVKFTPATLAGDGGLNKLADFLQAFCKLKLQHIQFNVVNAD
TLREAQQRPQDFAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
>Mature_764_residues
TNRTQRLKASLFAQPREISLERALLYTASHRQTEGEPVIIRRAKATAWILDKVMISIREDELIAGNRTVKPRAGIMSPEM
DPYWLLNELDAFPTRPQDRFAISEEDKQIYRETLYPYWEKRSMKDFINGQMTEEVKAAVNTQIFSVNQTDKGQGHIIIDY
PRLLNHGLGALVSELKTHCARQPENPFYQAVLILLEASQRHILRYAALAEEMAGHCQDPQRQQELLTIAAISRHNAQHQP
TDFPQACQLFWYMNIILQYESNASSISLGRFDQYMLPFYQASLNQGQDPAYLKELLESLWVKCNDIVLLRSSSSARYFAG
FPTGYTALLGGLTDTGRSAVNVLSFLCLDAYQNVQLPQPNLGVRVNELIDRPFLRKTAETIRLGTGIPQIFNDEVVVPAF
LNRGVSLEDARDYAVVGCVELSIPGRTYGLHDIAMFNLLKVMEIVLQENEHQPDVSWDGLICQIRDKIRHYIKLMVEGSN
ICDIGHRDWAPVPLLSSFIEDCVQHGKDITAGGARYNFSGVQGIGIANLSDSLYALKGMVFDQQRLSFDQLMAVLKANFQ
TPEGKKIRARLINRFDKYGNDIDDVDNISADLLRFYCKEVEQYLNPRGGHFTPGSYTVSAHVPLGSVVGATPDGRLAGEQ
LADGGLSPMVGQDSQGPTAVLKSVSKLDNYLLSNGTLLNVKFTPATLAGDGGLNKLADFLQAFCKLKLQHIQFNVVNADT
LREAQQRPQDFAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=765, Percent_Identity=85.6209150326797, Blast_Score=1364, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=787, Percent_Identity=37.2299872935197, Blast_Score=460, Evalue=1e-130,
Organism=Escherichia coli, GI48994926, Length=645, Percent_Identity=26.046511627907, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1787131, Length=639, Percent_Identity=25.1956181533646, Blast_Score=146, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 85795; Mature: 85664

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRTQRLKASLFAQPREISLERALLYTASHRQTEGEPVIIRRAKATAWILDKVMISIRE
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHCC
DELIAGNRTVKPRAGIMSPEMDPYWLLNELDAFPTRPQDRFAISEEDKQIYRETLYPYWE
CCEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCHHHH
KRSMKDFINGQMTEEVKAAVNTQIFSVNQTDKGQGHIIIDYPRLLNHGLGALVSELKTHC
HCCHHHHHCCCHHHHHHHHHCCEEEEECCCCCCCCEEEEECHHHHHCCHHHHHHHHHHHH
ARQPENPFYQAVLILLEASQRHILRYAALAEEMAGHCQDPQRQQELLTIAAISRHNAQHQ
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
PTDFPQACQLFWYMNIILQYESNASSISLGRFDQYMLPFYQASLNQGQDPAYLKELLESL
CCCCHHHHHHHHHHHHHEEECCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
WVKCNDIVLLRSSSSARYFAGFPTGYTALLGGLTDTGRSAVNVLSFLCLDAYQNVQLPQP
CCCCCCEEEEECCCCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
NLGVRVNELIDRPFLRKTAETIRLGTGIPQIFNDEVVVPAFLNRGVSLEDARDYAVVGCV
CCCEEHHHHHCCHHHHHHHHHHHHCCCCCHHHCCCEEEHHHHCCCCCCCCCCCEEEEEEE
ELSIPGRTYGLHDIAMFNLLKVMEIVLQENEHQPDVSWDGLICQIRDKIRHYIKLMVEGS
EEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
NICDIGHRDWAPVPLLSSFIEDCVQHGKDITAGGARYNFSGVQGIGIANLSDSLYALKGM
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEECCCHHHHHHHHH
VFDQQRLSFDQLMAVLKANFQTPEGKKIRARLINRFDKYGNDIDDVDNISADLLRFYCKE
HHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VEQYLNPRGGHFTPGSYTVSAHVPLGSVVGATPDGRLAGEQLADGGLSPMVGQDSQGPTA
HHHHHCCCCCCCCCCCEEEEECCCCCHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHH
VLKSVSKLDNYLLSNGTLLNVKFTPATLAGDGGLNKLADFLQAFCKLKLQHIQFNVVNAD
HHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEECHH
TLREAQQRPQDFAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
HHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNRTQRLKASLFAQPREISLERALLYTASHRQTEGEPVIIRRAKATAWILDKVMISIRE
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHHHHHHCC
DELIAGNRTVKPRAGIMSPEMDPYWLLNELDAFPTRPQDRFAISEEDKQIYRETLYPYWE
CCEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCHHHH
KRSMKDFINGQMTEEVKAAVNTQIFSVNQTDKGQGHIIIDYPRLLNHGLGALVSELKTHC
HCCHHHHHCCCHHHHHHHHHCCEEEEECCCCCCCCEEEEECHHHHHCCHHHHHHHHHHHH
ARQPENPFYQAVLILLEASQRHILRYAALAEEMAGHCQDPQRQQELLTIAAISRHNAQHQ
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
PTDFPQACQLFWYMNIILQYESNASSISLGRFDQYMLPFYQASLNQGQDPAYLKELLESL
CCCCHHHHHHHHHHHHHEEECCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
WVKCNDIVLLRSSSSARYFAGFPTGYTALLGGLTDTGRSAVNVLSFLCLDAYQNVQLPQP
CCCCCCEEEEECCCCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
NLGVRVNELIDRPFLRKTAETIRLGTGIPQIFNDEVVVPAFLNRGVSLEDARDYAVVGCV
CCCEEHHHHHCCHHHHHHHHHHHHCCCCCHHHCCCEEEHHHHCCCCCCCCCCCEEEEEEE
ELSIPGRTYGLHDIAMFNLLKVMEIVLQENEHQPDVSWDGLICQIRDKIRHYIKLMVEGS
EEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
NICDIGHRDWAPVPLLSSFIEDCVQHGKDITAGGARYNFSGVQGIGIANLSDSLYALKGM
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEEECCCHHHHHHHHH
VFDQQRLSFDQLMAVLKANFQTPEGKKIRARLINRFDKYGNDIDDVDNISADLLRFYCKE
HHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VEQYLNPRGGHFTPGSYTVSAHVPLGSVVGATPDGRLAGEQLADGGLSPMVGQDSQGPTA
HHHHHCCCCCCCCCCCEEEEECCCCCHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHH
VLKSVSKLDNYLLSNGTLLNVKFTPATLAGDGGLNKLADFLQAFCKLKLQHIQFNVVNAD
HHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEECHH
TLREAQQRPQDFAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
HHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]