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The map label for this gene is pflC [H]

Identifier: 238894042

GI number: 238894042

Start: 1939013

End: 1939888

Strand: Reverse

Name: pflC [H]

Synonym: KP1_1988

Alternate gene names: 238894042

Gene position: 1939888-1939013 (Counterclockwise)

Preceding gene: 238894043

Following gene: 238894041

Centisome position: 36.96

GC content: 59.25

Gene sequence:

>876_bases
ATGACATTATCCGCCGCACCGCGCATCAGCTGTGAGGTCATCGACACGCGCGCCGACCGGGCGCGTATTTTTAACCTCCA
GCGCTACTCGCTCAATGACGGACAAGGGATCCGCACGGTGGTCTTTTTTAAAGGCTGCCCTCATACCTGTCCGTGGTGCG
CCAATCCCGAATCAATTTCACCCAGGATTCAGACCCTGCGCCGGGAGAGCAAATGCCTGCGCTGCACCCGTTGCCAGCAG
GACGTTGCCGAATGTCCATCCGGAGCGTGGGAACAGATTGGCCGCGATGTGACGCTGGATAACCTGTTGCAGGAGGTGCT
GAAGGATGAGGTCTTCTTTCGCGCCTCCGGCGGCGGAGTCACCCTCTCCGGGGGCGAAGTGCTCATGCAGGCCGGGTTTG
CCGCCCGCTTTCTCCAGCGCCTGCGCCAGTGGGGGATCCGCACCGCCATCGAAACCGCGGGCGACAGCGCTTTTGACCGC
TTTCTGCCGGTGGCCGAAGCCTGTAATGAAGTGCTGTTTGATTTTAAAATCATGGAACCTGAGCGGGCGCGGTCGCTGCT
GCGAATGAATCAGCCACGGGTGCTGGACAATTTTCGCCAGCTGGCGGCGAGAAAAATCAACCTGATCCCGCGGGTGCCGC
TGATCCCCGGCTACACCCTGAATACCGATAATTTTCGCCAGATTCTGGCGTTCCTCGCCCCTTTCGCGCTAAAAGAAATG
CACCTGTTGCCGTTTCATCAATATGGCGAGCCCAAATACCGTCTGCTGGGCAAACCCTGGACGCTGGCCAGCGTGAAAGC
GCCTGACGAAGCGGAAATTCAACCATATCGGGTGATGGCCGAAGCGGCCGGTTTCCACGTCACTATCGGCGGTTAA

Upstream 100 bases:

>100_bases
CTGCGTGAAGCGCAGCAGCGGCCGCAGGACTTTGCCGGTCTGGTGGTACGGGTCGCGGGCTACAGCGCCTTCTTTGTTGA
GCTGTCGAAGGAGATTCAGG

Downstream 100 bases:

>100_bases
CGCCAGGAGGATGTTATGGACTTCAATATTGTCGCCGTCACCGCCTGCGTCAGCGGCGTGGCACATACCTACATGGCCGC
CGAACGACTGGAAAAGGTCG

Product: pyruvate formate lyase II activase

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 2; PFL-activating enzyme 2 [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTLSAAPRISCEVIDTRADRARIFNLQRYSLNDGQGIRTVVFFKGCPHTCPWCANPESISPRIQTLRRESKCLRCTRCQQ
DVAECPSGAWEQIGRDVTLDNLLQEVLKDEVFFRASGGGVTLSGGEVLMQAGFAARFLQRLRQWGIRTAIETAGDSAFDR
FLPVAEACNEVLFDFKIMEPERARSLLRMNQPRVLDNFRQLAARKINLIPRVPLIPGYTLNTDNFRQILAFLAPFALKEM
HLLPFHQYGEPKYRLLGKPWTLASVKAPDEAEIQPYRVMAEAAGFHVTIGG

Sequences:

>Translated_291_residues
MTLSAAPRISCEVIDTRADRARIFNLQRYSLNDGQGIRTVVFFKGCPHTCPWCANPESISPRIQTLRRESKCLRCTRCQQ
DVAECPSGAWEQIGRDVTLDNLLQEVLKDEVFFRASGGGVTLSGGEVLMQAGFAARFLQRLRQWGIRTAIETAGDSAFDR
FLPVAEACNEVLFDFKIMEPERARSLLRMNQPRVLDNFRQLAARKINLIPRVPLIPGYTLNTDNFRQILAFLAPFALKEM
HLLPFHQYGEPKYRLLGKPWTLASVKAPDEAEIQPYRVMAEAAGFHVTIGG
>Mature_290_residues
TLSAAPRISCEVIDTRADRARIFNLQRYSLNDGQGIRTVVFFKGCPHTCPWCANPESISPRIQTLRRESKCLRCTRCQQD
VAECPSGAWEQIGRDVTLDNLLQEVLKDEVFFRASGGGVTLSGGEVLMQAGFAARFLQRLRQWGIRTAIETAGDSAFDRF
LPVAEACNEVLFDFKIMEPERARSLLRMNQPRVLDNFRQLAARKINLIPRVPLIPGYTLNTDNFRQILAFLAPFALKEMH
LLPFHQYGEPKYRLLGKPWTLASVKAPDEAEIQPYRVMAEAAGFHVTIGG

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI1790389, Length=292, Percent_Identity=64.3835616438356, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI226510931, Length=268, Percent_Identity=35.8208955223881, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787130, Length=274, Percent_Identity=27.007299270073, Blast_Score=118, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006638
- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 32829; Mature: 32698

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSAAPRISCEVIDTRADRARIFNLQRYSLNDGQGIRTVVFFKGCPHTCPWCANPESIS
CCCCCCCCCEEEEECCCCCHHHEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
PRIQTLRRESKCLRCTRCQQDVAECPSGAWEQIGRDVTLDNLLQEVLKDEVFFRASGGGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHEEEEECCCCE
TLSGGEVLMQAGFAARFLQRLRQWGIRTAIETAGDSAFDRFLPVAEACNEVLFDFKIMEP
EECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCH
ERARSLLRMNQPRVLDNFRQLAARKINLIPRVPLIPGYTLNTDNFRQILAFLAPFALKEM
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHH
HLLPFHQYGEPKYRLLGKPWTLASVKAPDEAEIQPYRVMAEAAGFHVTIGG
HCCCCHHCCCCCEEECCCCEEEEECCCCCCCCCCHHHHHHHHCCEEEEECC
>Mature Secondary Structure 
TLSAAPRISCEVIDTRADRARIFNLQRYSLNDGQGIRTVVFFKGCPHTCPWCANPESIS
CCCCCCCCEEEEECCCCCHHHEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
PRIQTLRRESKCLRCTRCQQDVAECPSGAWEQIGRDVTLDNLLQEVLKDEVFFRASGGGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHEEEEECCCCE
TLSGGEVLMQAGFAARFLQRLRQWGIRTAIETAGDSAFDRFLPVAEACNEVLFDFKIMEP
EECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCH
ERARSLLRMNQPRVLDNFRQLAARKINLIPRVPLIPGYTLNTDNFRQILAFLAPFALKEM
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHH
HLLPFHQYGEPKYRLLGKPWTLASVKAPDEAEIQPYRVMAEAAGFHVTIGG
HCCCCHHCCCCCEEECCCCEEEEECCCCCCCCCCHHHHHHHHCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]