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The map label for this gene is yccW [H]

Identifier: 238894022

GI number: 238894022

Start: 1920576

End: 1921811

Strand: Reverse

Name: yccW [H]

Synonym: KP1_1968

Alternate gene names: 238894022

Gene position: 1921811-1920576 (Counterclockwise)

Preceding gene: 238894026

Following gene: 238894021

Centisome position: 36.62

GC content: 60.6

Gene sequence:

>1236_bases
GTGCCGACATTCCCGTTGCTTAAAGGTAGCCCCATGACAGATTCCCTATTCCCCCGCTTAGTGTTAGCCAAAGGACGCGA
GAAATCCCTGCTGCGTCGCCATCCGTGGATATTCTCCGGCGGCGTCGCCCGCATGGAAGGTAAAGCCCGCAGCGGTGAAA
CCATCGATATCGTTGACCATCAGGGAAAATGGCTGGCCCGCGGCGCTTACTCGCCGTCGTCGCAGATCCGCGCCCGCGTG
TGGACCTTCGACCGCAATGAAGCCATCGATAGCGCCTTCTTTGAACGCCGTCTGCAGCAGGCGCAAACCTGGCGTGCCTG
GCTGGCCGAGCGAGATGGCCTCGACAGCTACCGCCTGATCGCCGGCGAGTCCGATGGCCTGCCGGGCGTCACTATCGACC
GCTTTGGCAACTTTTTCGTGCTGCAGCTGCTCAGCGCAGGCGCCGAATACCAGCGCGCCGCCATCATCAGCGCCCTGCAG
AACCTGTTCCCGGACTGCGCCATTTACGATCGCAGCGATGTCGCCGTGCGTAAAAAAGAGGGACTGGAGCTGGCCCAGGG
GCCGGTGGTGGGCGAACTGCCCCCGGCGCTGCTGCCGATCACCGAGCACGGCATGAAGCTGTTAGTCGATATCCAGGGCG
GGCACAAGACCGGCTACTACCTCGACCAGCGCGACAGCCGCCTGGCCACCCGCCGCTATGTCGCGGATAAACGGGTGCTG
AACTGCTTCTCCTACACCGGCGGCTTTGCGGTGTCGGCGCTGATGGGCGGCTGCCGCCAGGTCACCAGCGTGGATACCTC
GCAGGAGGCGCTCGACGTTGCCCGCCAGAACGTGGAGATCAACGGACTGGATCTGTCCAAAGCGGAGTTTGTGCGCGACG
ACGTGTTCAAACTGCTGCGTAAATACCGCGATCAGGGAGAGAAATTCGACGTGATCGTGATGGATCCGCCGAAGTTCGTG
GAAAATAAAAGCCAGCTGATGGGCGCCTGCCGCGGCTATAAAGATATCAATATGCTGGCGATCCAGTTGCTGAACCCCGG
CGGCGTGCTGCTGACATTCTCCTGCTCCGGGCTGATGACCACCGATTTATTTCAGAAAATCATCGCCGATGCCGCAATTG
ATGCCGGGCGTGATGTACAATTTATAGAACAGTTCCGTCAGGCTGCCGACCACCCGGTGATCGCAACCTACCCGGAAGGG
CTGTATCTGAAGGGGTTTGCCTGTCGCGTCATGTAA

Upstream 100 bases:

>100_bases
TGCCGAAGCGCCTGCATTTTGTGCCGAAACGCTCACCTGTGCCAGCCGGGCGAAAGATGCTTGCGTGCGCCAGGTGTGCT
AATCTACGCGCCATTTTTCG

Downstream 100 bases:

>100_bases
CTTGAAAAACGAAACATGAGCCTCATATAGGGAGTATTCATGTTTCCCAGGAGGTGCCTATGATAGCCAGCAAATTCGGT
ATCGGCCAACAGGTCCGCCA

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MPTFPLLKGSPMTDSLFPRLVLAKGREKSLLRRHPWIFSGGVARMEGKARSGETIDIVDHQGKWLARGAYSPSSQIRARV
WTFDRNEAIDSAFFERRLQQAQTWRAWLAERDGLDSYRLIAGESDGLPGVTIDRFGNFFVLQLLSAGAEYQRAAIISALQ
NLFPDCAIYDRSDVAVRKKEGLELAQGPVVGELPPALLPITEHGMKLLVDIQGGHKTGYYLDQRDSRLATRRYVADKRVL
NCFSYTGGFAVSALMGGCRQVTSVDTSQEALDVARQNVEINGLDLSKAEFVRDDVFKLLRKYRDQGEKFDVIVMDPPKFV
ENKSQLMGACRGYKDINMLAIQLLNPGGVLLTFSCSGLMTTDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEG
LYLKGFACRVM

Sequences:

>Translated_411_residues
MPTFPLLKGSPMTDSLFPRLVLAKGREKSLLRRHPWIFSGGVARMEGKARSGETIDIVDHQGKWLARGAYSPSSQIRARV
WTFDRNEAIDSAFFERRLQQAQTWRAWLAERDGLDSYRLIAGESDGLPGVTIDRFGNFFVLQLLSAGAEYQRAAIISALQ
NLFPDCAIYDRSDVAVRKKEGLELAQGPVVGELPPALLPITEHGMKLLVDIQGGHKTGYYLDQRDSRLATRRYVADKRVL
NCFSYTGGFAVSALMGGCRQVTSVDTSQEALDVARQNVEINGLDLSKAEFVRDDVFKLLRKYRDQGEKFDVIVMDPPKFV
ENKSQLMGACRGYKDINMLAIQLLNPGGVLLTFSCSGLMTTDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEG
LYLKGFACRVM
>Mature_410_residues
PTFPLLKGSPMTDSLFPRLVLAKGREKSLLRRHPWIFSGGVARMEGKARSGETIDIVDHQGKWLARGAYSPSSQIRARVW
TFDRNEAIDSAFFERRLQQAQTWRAWLAERDGLDSYRLIAGESDGLPGVTIDRFGNFFVLQLLSAGAEYQRAAIISALQN
LFPDCAIYDRSDVAVRKKEGLELAQGPVVGELPPALLPITEHGMKLLVDIQGGHKTGYYLDQRDSRLATRRYVADKRVLN
CFSYTGGFAVSALMGGCRQVTSVDTSQEALDVARQNVEINGLDLSKAEFVRDDVFKLLRKYRDQGEKFDVIVMDPPKFVE
NKSQLMGACRGYKDINMLAIQLLNPGGVLLTFSCSGLMTTDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGL
YLKGFACRVM

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=393, Percent_Identity=90.3307888040712, Blast_Score=743, Evalue=0.0,
Organism=Escherichia coli, GI1787181, Length=243, Percent_Identity=30.0411522633745, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF01472 PUA [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 45829; Mature: 45698

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTFPLLKGSPMTDSLFPRLVLAKGREKSLLRRHPWIFSGGVARMEGKARSGETIDIVDH
CCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCEEECCCCEECCCCCCCCEEEEEEC
QGKWLARGAYSPSSQIRARVWTFDRNEAIDSAFFERRLQQAQTWRAWLAERDGLDSYRLI
CCCEEECCCCCCHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
AGESDGLPGVTIDRFGNFFVLQLLSAGAEYQRAAIISALQNLFPDCAIYDRSDVAVRKKE
ECCCCCCCCCCHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEHHC
GLELAQGPVVGELPPALLPITEHGMKLLVDIQGGHKTGYYLDQRDSRLATRRYVADKRVL
CCCHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHH
NCFSYTGGFAVSALMGGCRQVTSVDTSQEALDVARQNVEINGLDLSKAEFVRDDVFKLLR
HHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHH
KYRDQGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNPGGVLLTFSCSGLMT
HHHCCCCEEEEEEECCCHHHCCHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCHH
TDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCEEECCEEEECC
>Mature Secondary Structure 
PTFPLLKGSPMTDSLFPRLVLAKGREKSLLRRHPWIFSGGVARMEGKARSGETIDIVDH
CCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCEEECCCCEECCCCCCCCEEEEEEC
QGKWLARGAYSPSSQIRARVWTFDRNEAIDSAFFERRLQQAQTWRAWLAERDGLDSYRLI
CCCEEECCCCCCHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
AGESDGLPGVTIDRFGNFFVLQLLSAGAEYQRAAIISALQNLFPDCAIYDRSDVAVRKKE
ECCCCCCCCCCHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEHHC
GLELAQGPVVGELPPALLPITEHGMKLLVDIQGGHKTGYYLDQRDSRLATRRYVADKRVL
CCCHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHH
NCFSYTGGFAVSALMGGCRQVTSVDTSQEALDVARQNVEINGLDLSKAEFVRDDVFKLLR
HHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHH
KYRDQGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNPGGVLLTFSCSGLMT
HHHCCCCEEEEEEECCCHHHCCHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCHH
TDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFACRVM
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCCCEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA