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The map label for this gene is artJ [H]

Identifier: 238893921

GI number: 238893921

Start: 1797897

End: 1798628

Strand: Reverse

Name: artJ [H]

Synonym: KP1_1858

Alternate gene names: 238893921

Gene position: 1798628-1797897 (Counterclockwise)

Preceding gene: 238893922

Following gene: 238893908

Centisome position: 34.27

GC content: 54.23

Gene sequence:

>732_bases
ATGAAAAAGTTAGTTCTGGCTGCACTGCTCACCTCTTTCGCGTTCGGCGCCGCTGCCGCGGAAAAAATCAGCTTTGGCGT
CTCCGCAACCTATCCGCCGTTTGAATCGATGGACGCTAATAATCAGATTGTCGGCTTTGATATCGATCTGGCCCACGCGC
TGTGCAAACAAATGCAGGCAGAATGTACCTTTACCAACCACGCGTTTGACAGCCTGATCCCGGCGCTGAAGTTCAAGAAA
TATGATGCGGTGATTTCCGGAATGGATATTACGCCGGAGCGGAGCAAGCAGGTGGCCTTCACCGACCCGTACTACGCCAA
CTCGGCGCTGGTGATCGCCAAAAAAGATGCGTTCCATTCTTTCGATGACCTGAAAGGCAAACGGATCGGCATGGAAAACG
GCACCACCCACCAGAAATATCTGCAGGATAAGCATCCTGAGGTGAAGACCGTCGCCTATGACAGCTACCAGAATGCGATT
ATCGATTTGAAAAATGGCCGTATCGACGGCGTGTTCGGCGACACCGCGGTGGTCAACGAATGGCTGAAAACTAACCCGCA
GCTGGGGGCAGCCACGCCGAAAGTGACCGACCCGCAATACTTCGGCACCGGACTCGGCATTGCCGTGCGCCCGGATAACA
AGGCGCTGCTGGAGAAACTGAACGCCGCGCTGAAAGCGATTAAGGCCGACGGTACCTACCAGAAAATTAGCAACCAGTGG
TTCCCGGAGTAA

Upstream 100 bases:

>100_bases
CTTAATCATTTTTATTGCATATAAATTCATTTAATGGCATTGTCACTCTATGCCGCAGACACGGTGAATAATCATAAGAT
AGACAGACGGGAGTTCCACG

Downstream 100 bases:

>100_bases
CTTTTTTCGCCGGGTGGCGCTGCGCTTACCCGGCCACCGCTCTTCCCCGTTGGCCGGGTAAGGCGTAGCCGCCACCCGGC
GATTTCTCAAAGTGGCACGA

Product: arginine 3rd transport system periplasmic binding component

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKKLVLAALLTSFAFGAAAAEKISFGVSATYPPFESMDANNQIVGFDIDLAHALCKQMQAECTFTNHAFDSLIPALKFKK
YDAVISGMDITPERSKQVAFTDPYYANSALVIAKKDAFHSFDDLKGKRIGMENGTTHQKYLQDKHPEVKTVAYDSYQNAI
IDLKNGRIDGVFGDTAVVNEWLKTNPQLGAATPKVTDPQYFGTGLGIAVRPDNKALLEKLNAALKAIKADGTYQKISNQW
FPE

Sequences:

>Translated_243_residues
MKKLVLAALLTSFAFGAAAAEKISFGVSATYPPFESMDANNQIVGFDIDLAHALCKQMQAECTFTNHAFDSLIPALKFKK
YDAVISGMDITPERSKQVAFTDPYYANSALVIAKKDAFHSFDDLKGKRIGMENGTTHQKYLQDKHPEVKTVAYDSYQNAI
IDLKNGRIDGVFGDTAVVNEWLKTNPQLGAATPKVTDPQYFGTGLGIAVRPDNKALLEKLNAALKAIKADGTYQKISNQW
FPE
>Mature_243_residues
MKKLVLAALLTSFAFGAAAAEKISFGVSATYPPFESMDANNQIVGFDIDLAHALCKQMQAECTFTNHAFDSLIPALKFKK
YDAVISGMDITPERSKQVAFTDPYYANSALVIAKKDAFHSFDDLKGKRIGMENGTTHQKYLQDKHPEVKTVAYDSYQNAI
IDLKNGRIDGVFGDTAVVNEWLKTNPQLGAATPKVTDPQYFGTGLGIAVRPDNKALLEKLNAALKAIKADGTYQKISNQW
FPE

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=243, Percent_Identity=88.0658436213992, Blast_Score=452, Evalue=1e-129,
Organism=Escherichia coli, GI1787088, Length=241, Percent_Identity=65.1452282157676, Blast_Score=345, Evalue=1e-96,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=40.2597402597403, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1788649, Length=253, Percent_Identity=38.3399209486166, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI1788228, Length=220, Percent_Identity=31.3636363636364, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1787031, Length=244, Percent_Identity=32.3770491803279, Blast_Score=115, Evalue=2e-27,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26678; Mature: 26678

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVLAALLTSFAFGAAAAEKISFGVSATYPPFESMDANNQIVGFDIDLAHALCKQMQA
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
ECTFTNHAFDSLIPALKFKKYDAVISGMDITPERSKQVAFTDPYYANSALVIAKKDAFHS
HCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCC
FDDLKGKRIGMENGTTHQKYLQDKHPEVKTVAYDSYQNAIIDLKNGRIDGVFGDTAVVNE
HHHCCCCEECCCCCCCHHHHHHHCCCCEEEEEECCCCCEEEEECCCEECCEECCHHHHHH
WLKTNPQLGAATPKVTDPQYFGTGLGIAVRPDNKALLEKLNAALKAIKADGTYQKISNQW
HHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHCCCC
FPE
CCC
>Mature Secondary Structure
MKKLVLAALLTSFAFGAAAAEKISFGVSATYPPFESMDANNQIVGFDIDLAHALCKQMQA
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
ECTFTNHAFDSLIPALKFKKYDAVISGMDITPERSKQVAFTDPYYANSALVIAKKDAFHS
HCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCC
FDDLKGKRIGMENGTTHQKYLQDKHPEVKTVAYDSYQNAIIDLKNGRIDGVFGDTAVVNE
HHHCCCCEECCCCCCCHHHHHHHCCCCEEEEEECCCCCEEEEECCCEECCEECCHHHHHH
WLKTNPQLGAATPKVTDPQYFGTGLGIAVRPDNKALLEKLNAALKAIKADGTYQKISNQW
HHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHCCCC
FPE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]