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The map label for this gene is ybiY [H]

Identifier: 238893884

GI number: 238893884

Start: 1759066

End: 1759965

Strand: Reverse

Name: ybiY [H]

Synonym: KP1_1817

Alternate gene names: 238893884

Gene position: 1759965-1759066 (Counterclockwise)

Preceding gene: 238893885

Following gene: 238893883

Centisome position: 33.53

GC content: 60.44

Gene sequence:

>900_bases
ATGATCTTCAACATCCAGCGCTATTCCACCCATGACGGCCCAGGGATCCGCACCGTCGTCTTCCTGAAAGGCTGCTCGCT
GGGCTGCCGCTGGTGTCAGAACCCGGAGAGCCGCGCCCGTAGCGAAGATCTGCTGTATGACTCGCGGCTGTGCCTGGCCG
GCTGCGAGCTGTGCCAGCAGGCGGCGCCGGCGGTTATCACCCGCACCCTCGATGGCCTGATCATTCACCGGCAAAACGTT
AACGACAGCCATATCGCCGCGCTGCGCGACTGCTGCCCCACCACCGCGCTCACGGTGTGCGGCGAGGAGAAGAGCGTCGA
GGAGATCATGGCGACGGTGCTGCGTGATAAACCATTCTACGATCGCAGCGGCGGCGGCATCACTCTTTCCGGCGGCGAAC
CCTTTATGAACCCAACGCTGGCGCAAGCGCTGTTCGAAGCCAGTCATCAGGCAGGCATTCACACCGCCGTGGAAACCTGC
CTGCACGTGCCGTGGAAATACATTGAACCGTCTTTACCCTTTGTCGACCTGTTTCTCGCCGATCTGAAGCACGTTGACGA
GGCCGTTTTTCAGCAGTGGACCGACGGCAGCGCCCGCCGGGTGCTGGATAACCTGCAGCGTCTGGCGCAGGCGGGGAAAA
AAATGATTATTCGCGTGCCGCTGATCCAGGGATTCAACGCCAGCGAAGCCGACATTGCCGCGATCACCGATTTTGCCGCC
GATCGCCTGCAGGTCAGCGAGATCCATTACCTGCCGTACCACACGCTGGGAATGAACAAATACCAGTTACTCAGTCAGCC
CTACACCGCACCGGACAAACCGCTGGACGCTCCTGAGCTGCTCGTCTTCGCGCAGGATTATGCGCAAAGCAAGGGGCTAA
CGGCGATATTACGAGGATAA

Upstream 100 bases:

>100_bases
TCTCCTCTCTGACTTATCCCCATCCCGCTTGTTGATTTGCGTCAACATAACTTTCATTCGAAAGTGTTTTTATATTCATA
CGCAAACAAGAGGATAAGCC

Downstream 100 bases:

>100_bases
CCCTATGACTACCCTGAAGCTCAACACCCTCAGCGCCCGTATCCAGGCCCATAAAATGGCGCTGGTGCATATCGTCAAGC
CGCCGGTCTGTACCGAGCGC

Product: putative pyruvate formate lyase activating enzyme

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 3; PFL-activating enzyme 3 [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARSEDLLYDSRLCLAGCELCQQAAPAVITRTLDGLIIHRQNV
NDSHIAALRDCCPTTALTVCGEEKSVEEIMATVLRDKPFYDRSGGGITLSGGEPFMNPTLAQALFEASHQAGIHTAVETC
LHVPWKYIEPSLPFVDLFLADLKHVDEAVFQQWTDGSARRVLDNLQRLAQAGKKMIIRVPLIQGFNASEADIAAITDFAA
DRLQVSEIHYLPYHTLGMNKYQLLSQPYTAPDKPLDAPELLVFAQDYAQSKGLTAILRG

Sequences:

>Translated_299_residues
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARSEDLLYDSRLCLAGCELCQQAAPAVITRTLDGLIIHRQNV
NDSHIAALRDCCPTTALTVCGEEKSVEEIMATVLRDKPFYDRSGGGITLSGGEPFMNPTLAQALFEASHQAGIHTAVETC
LHVPWKYIEPSLPFVDLFLADLKHVDEAVFQQWTDGSARRVLDNLQRLAQAGKKMIIRVPLIQGFNASEADIAAITDFAA
DRLQVSEIHYLPYHTLGMNKYQLLSQPYTAPDKPLDAPELLVFAQDYAQSKGLTAILRG
>Mature_299_residues
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARSEDLLYDSRLCLAGCELCQQAAPAVITRTLDGLIIHRQNV
NDSHIAALRDCCPTTALTVCGEEKSVEEIMATVLRDKPFYDRSGGGITLSGGEPFMNPTLAQALFEASHQAGIHTAVETC
LHVPWKYIEPSLPFVDLFLADLKHVDEAVFQQWTDGSARRVLDNLQRLAQAGKKMIIRVPLIQGFNASEADIAAITDFAA
DRLQVSEIHYLPYHTLGMNKYQLLSQPYTAPDKPLDAPELLVFAQDYAQSKGLTAILRG

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI226510931, Length=299, Percent_Identity=78.5953177257525, Blast_Score=483, Evalue=1e-138,
Organism=Escherichia coli, GI1790389, Length=298, Percent_Identity=32.5503355704698, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787130, Length=270, Percent_Identity=25.9259259259259, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790839, Length=231, Percent_Identity=27.7056277056277, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 33090; Mature: 33090

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARSEDLLYDSRLCLAGCELCQQ
CEEEEEECCCCCCCCCEEEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAPAVITRTLDGLIIHRQNVNDSHIAALRDCCPTTALTVCGEEKSVEEIMATVLRDKPFY
HHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHCCCCC
DRSGGGITLSGGEPFMNPTLAQALFEASHQAGIHTAVETCLHVPWKYIEPSLPFVDLFLA
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCHHHHHHH
DLKHVDEAVFQQWTDGSARRVLDNLQRLAQAGKKMIIRVPLIQGFNASEADIAAITDFAA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
DRLQVSEIHYLPYHTLGMNKYQLLSQPYTAPDKPLDAPELLVFAQDYAQSKGLTAILRG
HHEEHHHEEECCCHHCCCCHHHHHCCCCCCCCCCCCCHHHEEEEHHHHCCCCCEEEECC
>Mature Secondary Structure
MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARSEDLLYDSRLCLAGCELCQQ
CEEEEEECCCCCCCCCEEEEEEECCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AAPAVITRTLDGLIIHRQNVNDSHIAALRDCCPTTALTVCGEEKSVEEIMATVLRDKPFY
HHHHHHHHHHCCEEEEECCCCHHHHHHHHHHCCHHHEEECCCHHHHHHHHHHHHHCCCCC
DRSGGGITLSGGEPFMNPTLAQALFEASHQAGIHTAVETCLHVPWKYIEPSLPFVDLFLA
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCHHHHHHH
DLKHVDEAVFQQWTDGSARRVLDNLQRLAQAGKKMIIRVPLIQGFNASEADIAAITDFAA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHH
DRLQVSEIHYLPYHTLGMNKYQLLSQPYTAPDKPLDAPELLVFAQDYAQSKGLTAILRG
HHEEHHHEEECCCHHCCCCHHHHHCCCCCCCCCCCCCHHHEEEEHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]