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The map label for this gene is entE [H]

Identifier: 238893644

GI number: 238893644

Start: 1503519

End: 1505126

Strand: Direct

Name: entE [H]

Synonym: KP1_1558

Alternate gene names: 238893644

Gene position: 1503519-1505126 (Clockwise)

Preceding gene: 238893643

Following gene: 238893645

Centisome position: 28.65

GC content: 62.19

Gene sequence:

>1608_bases
ATGATTGCATTTACCCGTTGGCCGGAAGAGTTTGCGGCCCGCTATCGTCAAAAAGGCTACTGGCAGGATCTGCCGTTAAC
CAATCTGATCACCCGTCACGCGGAAAACGACGCCGTGGCGATTATCGATGGCGAGCGGCAGATTAGCTACCGCCAGTTTA
ACCAGCTGGTGGACAACCTCGCCTGCTCGCTGCAGCGCCAGGGACTGAAGCGCGGGGAGACCGCGCTGGTGCAGTTGGGC
AATGTCGCCGAGTTTTACATGACCTTCTTTGCGCTGCTGCGCATCGGCGTCGCGCCAGTGAACGCTTTGTTCAGCCACCA
GCGCAGCGAACTTAATGCCTACGCCGCGCAGATTAAACCGGCGCTGCTGATTGCCGATCGCGAGCACGCGCTGTTTGCTG
ACGATAGCTTTCTCCACGCGTTTATCGCGGAACATCCTTCCCTGCGCGTCGCACTGTTGCGTAACGACGGCGGCGAGCGC
GATCTGGCGACGGAGATTAACCGCCCGGCGGATAACTTTATCGCCAATCCAACCCCTGCCGACGAAGTGGCCTTTTTCCA
GCTCTCCGGCGGCAGCACCGGGACGCCGAAGCTTATCCCACGCACCCATAACGATTACGACTACAGTATCCGCCGCAGCA
ACGAGATCTGCGGGATTAACGCTGAAACGCGCTACCTCAACGCGCTGCCGGCGGCGCATAACTATGCGATGAGTTCCCCG
GGTTCGCTCGGCGTCTTCCTCGCCGGCGGGCGGGTGATCCTCGCCGCCGACCCGAGCGCCACGCTCTGTTTCCCGCTGAT
CGAAAAACATCAGATCAACGTTGCCTCGCTGGTGCCGCCGGCGGTCAGCCTGTGGCTGCAGGCGATTCACGAATGGGGCA
GCAACGCCCAGCTGCAATCGCTGCAGCTGCTGCAGGTCGGCGGCGCGCGCCTGTCAGCGACCCTCGCCGCGCGCATTCCG
GCGGAAATCGGCTGCCAGCTGCAGCAGGTGTTCGGCATGGCGGAGGGGCTGGTGAATTACACCCGCCTTAACGATAGCCC
GGAGCGGATCATCAATACCCAGGGCTGTCCGATGTGCCCGGACGATGAAGTGTGGGTAGCGGATGCCGACGGCAACCCGC
TGCCGCGCGGCGAAGTGGGCCGCCTGATGACCCGCGGGCCGTACACTTTCCGCGGCTACTACAACAGCCCGCAGCATAAC
GCGGAAGCCTTTGACGCCGAGGGGTTTTATTGCTCCGGCGATCTGATTTCGATCGACGAAGATGGGTATATCACCGTCCA
GGGCCGGGAAAAAGATCAGATCAACCGCGGCGGCGAGAAGATCGCCGCTGAAGAGATCGAAAACCTGCTGCTGCGCCATG
AGGCGGTGATCCACGCCGCGCTGGTCAGCATCGAAGACAATCTGCTGGGCGAGAAAAGCTGCGCTTATCTGGTGGTGACC
TCGCCGCTGCGCGCCGTCGCCGTGCGCCGCTTCCTGCGCGAGCAGGGGGTGGCGGAATTTAAACTGCCGGACCGCGTGGA
GTGCGTTGCCGCGCTGCCGCTGACGCCGGTGGGCAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCCGAAGGCAAGC
TGGGCTGA

Upstream 100 bases:

>100_bases
GGGCATTGTTCCGGCCTCCTCGCCGCTGGGCGAATGGCGCGAAACCGGCGTCAAACTGACCACCATGCTTAACGTATTTG
GCTTGAACTAAGAGGCAAGG

Downstream 100 bases:

>100_bases
AGGAGAACAGAGAATGGCAATCCCTAAATTACAGGCATATGCGCTGCCGGAAGCCAGCGATATCCCGGCGAACAAGGTCA
ACTGGGCCTTTGAGCCGTCG

Product: enterobactin synthase subunit E

Products: NA

Alternate protein names: Enterochelin synthase E; 2,3-dihydroxybenzoate-AMP ligase; Dihydroxybenzoic acid-activating enzyme; S-dihydroxybenzoyltransferase [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MIAFTRWPEEFAARYRQKGYWQDLPLTNLITRHAENDAVAIIDGERQISYRQFNQLVDNLACSLQRQGLKRGETALVQLG
NVAEFYMTFFALLRIGVAPVNALFSHQRSELNAYAAQIKPALLIADREHALFADDSFLHAFIAEHPSLRVALLRNDGGER
DLATEINRPADNFIANPTPADEVAFFQLSGGSTGTPKLIPRTHNDYDYSIRRSNEICGINAETRYLNALPAAHNYAMSSP
GSLGVFLAGGRVILAADPSATLCFPLIEKHQINVASLVPPAVSLWLQAIHEWGSNAQLQSLQLLQVGGARLSATLAARIP
AEIGCQLQQVFGMAEGLVNYTRLNDSPERIINTQGCPMCPDDEVWVADADGNPLPRGEVGRLMTRGPYTFRGYYNSPQHN
AEAFDAEGFYCSGDLISIDEDGYITVQGREKDQINRGGEKIAAEEIENLLLRHEAVIHAALVSIEDNLLGEKSCAYLVVT
SPLRAVAVRRFLREQGVAEFKLPDRVECVAALPLTPVGKVDKKQLRQWLAEGKLG

Sequences:

>Translated_535_residues
MIAFTRWPEEFAARYRQKGYWQDLPLTNLITRHAENDAVAIIDGERQISYRQFNQLVDNLACSLQRQGLKRGETALVQLG
NVAEFYMTFFALLRIGVAPVNALFSHQRSELNAYAAQIKPALLIADREHALFADDSFLHAFIAEHPSLRVALLRNDGGER
DLATEINRPADNFIANPTPADEVAFFQLSGGSTGTPKLIPRTHNDYDYSIRRSNEICGINAETRYLNALPAAHNYAMSSP
GSLGVFLAGGRVILAADPSATLCFPLIEKHQINVASLVPPAVSLWLQAIHEWGSNAQLQSLQLLQVGGARLSATLAARIP
AEIGCQLQQVFGMAEGLVNYTRLNDSPERIINTQGCPMCPDDEVWVADADGNPLPRGEVGRLMTRGPYTFRGYYNSPQHN
AEAFDAEGFYCSGDLISIDEDGYITVQGREKDQINRGGEKIAAEEIENLLLRHEAVIHAALVSIEDNLLGEKSCAYLVVT
SPLRAVAVRRFLREQGVAEFKLPDRVECVAALPLTPVGKVDKKQLRQWLAEGKLG
>Mature_535_residues
MIAFTRWPEEFAARYRQKGYWQDLPLTNLITRHAENDAVAIIDGERQISYRQFNQLVDNLACSLQRQGLKRGETALVQLG
NVAEFYMTFFALLRIGVAPVNALFSHQRSELNAYAAQIKPALLIADREHALFADDSFLHAFIAEHPSLRVALLRNDGGER
DLATEINRPADNFIANPTPADEVAFFQLSGGSTGTPKLIPRTHNDYDYSIRRSNEICGINAETRYLNALPAAHNYAMSSP
GSLGVFLAGGRVILAADPSATLCFPLIEKHQINVASLVPPAVSLWLQAIHEWGSNAQLQSLQLLQVGGARLSATLAARIP
AEIGCQLQQVFGMAEGLVNYTRLNDSPERIINTQGCPMCPDDEVWVADADGNPLPRGEVGRLMTRGPYTFRGYYNSPQHN
AEAFDAEGFYCSGDLISIDEDGYITVQGREKDQINRGGEKIAAEEIENLLLRHEAVIHAALVSIEDNLLGEKSCAYLVVT
SPLRAVAVRRFLREQGVAEFKLPDRVECVAALPLTPVGKVDKKQLRQWLAEGKLG

Specific function: Activates the carboxylate group of 2,3-dihydroxy- benzoate (2,3-DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate. Then, catalyzes the acylation of holo-EntB with 2,3-DHB adenylate, preparing that molecule for amide bond formation with

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. EntE subfamily [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=492, Percent_Identity=26.0162601626016, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI122937307, Length=526, Percent_Identity=24.7148288973384, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI157311624, Length=489, Percent_Identity=23.9263803680982, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI157311622, Length=489, Percent_Identity=23.9263803680982, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI58082049, Length=496, Percent_Identity=24.7983870967742, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI42544132, Length=502, Percent_Identity=22.9083665338645, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI115511026, Length=554, Percent_Identity=24.5487364620939, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI156151445, Length=551, Percent_Identity=21.5970961887477, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI187761345, Length=568, Percent_Identity=22.7112676056338, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI187761343, Length=568, Percent_Identity=22.7112676056338, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI28416953, Length=525, Percent_Identity=22.2857142857143, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI8923896, Length=400, Percent_Identity=22.5, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI253970489, Length=400, Percent_Identity=22.5, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI57165412, Length=341, Percent_Identity=22.5806451612903, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI40807491, Length=193, Percent_Identity=29.0155440414508, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI57165410, Length=341, Percent_Identity=22.5806451612903, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI1786810, Length=529, Percent_Identity=81.2854442344045, Blast_Score=905, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=535, Percent_Identity=29.5327102803738, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI221142682, Length=497, Percent_Identity=24.748490945674, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1788107, Length=539, Percent_Identity=24.1187384044527, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1790505, Length=558, Percent_Identity=23.8351254480287, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1786529, Length=562, Percent_Identity=24.0213523131673, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789201, Length=363, Percent_Identity=21.763085399449, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786801, Length=536, Percent_Identity=22.9477611940299, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17557194, Length=546, Percent_Identity=25.6410256410256, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=540, Percent_Identity=25.3703703703704, Blast_Score=125, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI71994694, Length=524, Percent_Identity=23.4732824427481, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71994703, Length=524, Percent_Identity=23.4732824427481, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71994690, Length=524, Percent_Identity=23.4732824427481, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17560308, Length=536, Percent_Identity=23.8805970149254, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17558820, Length=504, Percent_Identity=24.2063492063492, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17531443, Length=528, Percent_Identity=21.4015151515152, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI32563687, Length=498, Percent_Identity=23.2931726907631, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=364, Percent_Identity=27.7472527472527, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=524, Percent_Identity=21.7557251908397, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71982997, Length=543, Percent_Identity=22.6519337016575, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71983001, Length=543, Percent_Identity=22.6519337016575, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560140, Length=302, Percent_Identity=26.8211920529801, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=536, Percent_Identity=23.134328358209, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24581924, Length=551, Percent_Identity=23.7749546279492, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI19922652, Length=529, Percent_Identity=24.7637051039698, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI21355181, Length=522, Percent_Identity=23.9463601532567, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356947, Length=463, Percent_Identity=23.5421166306695, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI18859661, Length=376, Percent_Identity=23.936170212766, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24656500, Length=493, Percent_Identity=24.1379310344828, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21355225, Length=584, Percent_Identity=22.4315068493151, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24667955, Length=576, Percent_Identity=21.1805555555556, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI62472339, Length=551, Percent_Identity=21.0526315789474, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24667959, Length=150, Percent_Identity=29.3333333333333, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648260, Length=271, Percent_Identity=24.7232472324723, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =2.7.7.58 [H]

Molecular weight: Translated: 59019; Mature: 59019

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAFTRWPEEFAARYRQKGYWQDLPLTNLITRHAENDAVAIIDGERQISYRQFNQLVDNL
CCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHH
ACSLQRQGLKRGETALVQLGNVAEFYMTFFALLRIGVAPVNALFSHQRSELNAYAAQIKP
HHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ALLIADREHALFADDSFLHAFIAEHPSLRVALLRNDGGERDLATEINRPADNFIANPTPA
EEEEECCCCEEECCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCHHHCCCCCCCC
DEVAFFQLSGGSTGTPKLIPRTHNDYDYSIRRSNEICGINAETRYLNALPAAHNYAMSSP
CCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCEECCCCCHHHHHCCCCCCCCCCCCC
GSLGVFLAGGRVILAADPSATLCFPLIEKHQINVASLVPPAVSLWLQAIHEWGSNAQLQS
CCEEEEEECCEEEEEECCCCEEEHHHHHHCCCCHHHHCCHHHHHHHHHHHHHCCCCCHHH
LQLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLNDSPERIINTQGCPMCP
HHHHHHCCCHHEEHHHHHCCHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC
DDEVWVADADGNPLPRGEVGRLMTRGPYTFRGYYNSPQHNAEAFDAEGFYCSGDLISIDE
CCCEEEECCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEECCCEEEECC
DGYITVQGREKDQINRGGEKIAAEEIENLLLRHEAVIHAALVSIEDNLLGEKSCAYLVVT
CCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
SPLRAVAVRRFLREQGVAEFKLPDRVECVAALPLTPVGKVDKKQLRQWLAEGKLG
CCHHHHHHHHHHHHCCCEEECCCCHHHEEEECCCCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MIAFTRWPEEFAARYRQKGYWQDLPLTNLITRHAENDAVAIIDGERQISYRQFNQLVDNL
CCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHH
ACSLQRQGLKRGETALVQLGNVAEFYMTFFALLRIGVAPVNALFSHQRSELNAYAAQIKP
HHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ALLIADREHALFADDSFLHAFIAEHPSLRVALLRNDGGERDLATEINRPADNFIANPTPA
EEEEECCCCEEECCCHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCHHHCCCCCCCC
DEVAFFQLSGGSTGTPKLIPRTHNDYDYSIRRSNEICGINAETRYLNALPAAHNYAMSSP
CCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCEECCCCCHHHHHCCCCCCCCCCCCC
GSLGVFLAGGRVILAADPSATLCFPLIEKHQINVASLVPPAVSLWLQAIHEWGSNAQLQS
CCEEEEEECCEEEEEECCCCEEEHHHHHHCCCCHHHHCCHHHHHHHHHHHHHCCCCCHHH
LQLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLNDSPERIINTQGCPMCP
HHHHHHCCCHHEEHHHHHCCHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC
DDEVWVADADGNPLPRGEVGRLMTRGPYTFRGYYNSPQHNAEAFDAEGFYCSGDLISIDE
CCCEEEECCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEECCCEEEECC
DGYITVQGREKDQINRGGEKIAAEEIENLLLRHEAVIHAALVSIEDNLLGEKSCAYLVVT
CCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
SPLRAVAVRRFLREQGVAEFKLPDRVECVAALPLTPVGKVDKKQLRQWLAEGKLG
CCHHHHHHHHHHHHCCCEEECCCCHHHEEEECCCCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]