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The map label for this gene is proB [H]

Identifier: 238893260

GI number: 238893260

Start: 1103620

End: 1104723

Strand: Reverse

Name: proB [H]

Synonym: KP1_1133

Alternate gene names: 238893260

Gene position: 1104723-1103620 (Counterclockwise)

Preceding gene: 238893261

Following gene: 238893259

Centisome position: 21.05

GC content: 63.32

Gene sequence:

>1104_bases
ATGAGTGAAAGCCAGACGCTGGTGGTAAAACTGGGCACCAGTGTTTTAACAGGCGGGTCCCGTCGCCTGAACCGCGCCCA
TATCGTCGAGCTTGTGCGCCAGTGCGCACAGTTGCACGCCATGGGACACCGTATTGTCATCGTGACCTCCGGGGCCATTG
CCGCCGGGCGCGAGCACCTTGGTTACCCGGAACTGCCCGCCACCATCGCCTCCAAGCAGCTGCTGGCGGCGGTGGGACAA
AGCCGTCTGATCCAGCTGTGGGAACAGCTGTTTTCTATCTATGGCATTCACGTTGGCCAGATGCTGCTGACCCGCGCCGA
CATGGAAGACAGAGAACGGTTCCTCAACGCCCGCGACACTCTGCGCGCGCTGCTGGATAACAGCATCGTCCCGGTCATTA
ACGAAAACGACGCCGTCGCCACCGCGGAAATCAAAGTGGGCGATAACGACAATCTCTCCGCGCTGGCCGCGATCCTCGCC
GGGGCGGATAAGCTGCTGCTGTTAACCGACCAGCCAGGCCTGTTCACCGCCGACCCGCGCAGCAACCCGCAGGCGGAGCT
GATTAAAGACGTCTACGGCATCGACGACGCGCTGCGCGCCATCGCCGGCGATAGCGTCTCCGGACTCGGCACCGGCGGCA
TGGGCACCAAGCTGCAGGCCGCGGACGTCGCCTGCCGCGCGGGGATTGATACCATCATCGCCGCAGGCAACCGTCCGGAC
GTCATCGGCCATGCGATGGCAGGCCTGCCGGTAGGTACCTGCTTCCACGCCCAGGAGTCTCCGCTGGAGAACCGTAAGCG
CTGGATCTTCGGCGCGCCGCCGGCGGGGGAAATCACCGTCGATGCGGGCGCGACCCAGGCGATCCTCGAAAGAGGCAGCT
CGCTGTTGCCAAAAGGGATCAAAATCGTCAGCGGCAACTTCTCCCGCGGCGAAGTGATCCGCATCCGCAATAGCGAAGGA
CGCGACATCGCTCACGGCGTCAGCCGCTACAACAGCGATGCGCTGCGCCTTATCGCCGGCCAGCACTCGCAGCAAATCGA
TGCCATTCTGGGCTATGAATACGGCCCGGTGGCCGTCCACCGCGATGATATGATCATCCGTTAA

Upstream 100 bases:

>100_bases
AATAAAATGGCGCGCCCGCCGCCCCGGGTGTTGGCAAGCGGGCCGACTCCTGCTACAACATCAGCTCGATATCGATATTC
CTTTTGAACGGCAGAGAATC

Downstream 100 bases:

>100_bases
GGAGCTGAAAATGCTGGAACAAATGGGCATTGCCGCCAAAGCGGCCTCCTGGCAGCTGGCGTTACTCTCCAGCCGGGAAA
AGAATCAGGTACTGGAAAAG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSESQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAMGHRIVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQ
SRLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDTLRALLDNSIVPVINENDAVATAEIKVGDNDNLSALAAILA
GADKLLLLTDQPGLFTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMGTKLQAADVACRAGIDTIIAAGNRPD
VIGHAMAGLPVGTCFHAQESPLENRKRWIFGAPPAGEITVDAGATQAILERGSSLLPKGIKIVSGNFSRGEVIRIRNSEG
RDIAHGVSRYNSDALRLIAGQHSQQIDAILGYEYGPVAVHRDDMIIR

Sequences:

>Translated_367_residues
MSESQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAMGHRIVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQ
SRLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDTLRALLDNSIVPVINENDAVATAEIKVGDNDNLSALAAILA
GADKLLLLTDQPGLFTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMGTKLQAADVACRAGIDTIIAAGNRPD
VIGHAMAGLPVGTCFHAQESPLENRKRWIFGAPPAGEITVDAGATQAILERGSSLLPKGIKIVSGNFSRGEVIRIRNSEG
RDIAHGVSRYNSDALRLIAGQHSQQIDAILGYEYGPVAVHRDDMIIR
>Mature_366_residues
SESQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAMGHRIVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQS
RLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDTLRALLDNSIVPVINENDAVATAEIKVGDNDNLSALAAILAG
ADKLLLLTDQPGLFTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMGTKLQAADVACRAGIDTIIAAGNRPDV
IGHAMAGLPVGTCFHAQESPLENRKRWIFGAPPAGEITVDAGATQAILERGSSLLPKGIKIVSGNFSRGEVIRIRNSEGR
DIAHGVSRYNSDALRLIAGQHSQQIDAILGYEYGPVAVHRDDMIIR

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=288, Percent_Identity=33.6805555555556, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI21361368, Length=290, Percent_Identity=33.448275862069, Blast_Score=129, Evalue=6e-30,
Organism=Escherichia coli, GI1786437, Length=367, Percent_Identity=92.6430517711172, Blast_Score=681, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17569969, Length=272, Percent_Identity=34.1911764705882, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17569967, Length=272, Percent_Identity=34.1911764705882, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321822, Length=418, Percent_Identity=36.1244019138756, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6320506, Length=419, Percent_Identity=34.6062052505967, Blast_Score=214, Evalue=1e-56,
Organism=Drosophila melanogaster, GI21357643, Length=294, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39117; Mature: 38986

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAMGHRIVIVTSGAIAAGREHL
CCCCCEEEEEECCHHHCCCCHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHHC
GYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LRALLDNSIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQPGLFTADPR
HHHHHCCCCEEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCEEECCCC
SNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMGTKLQAADVACRAGIDTIIAAGNRPD
CCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHCCCHHEEECCCCCC
VIGHAMAGLPVGTCFHAQESPLENRKRWIFGAPPAGEITVDAGATQAILERGSSLLPKGI
HHHHHHHCCCCCEEECCCCCCCCCCCCEEEECCCCCCEEECCCCHHHHHHCCCCCCCCCE
KIVSGNFSRGEVIRIRNSEGRDIAHGVSRYNSDALRLIAGQHSQQIDAILGYEYGPVAVH
EEEECCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCCCCEEEE
RDDMIIR
CCCEEEC
>Mature Secondary Structure 
SESQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAMGHRIVIVTSGAIAAGREHL
CCCCEEEEEECCHHHCCCCHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHHC
GYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LRALLDNSIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQPGLFTADPR
HHHHHCCCCEEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCEEECCCC
SNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMGTKLQAADVACRAGIDTIIAAGNRPD
CCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHCCCHHEEECCCCCC
VIGHAMAGLPVGTCFHAQESPLENRKRWIFGAPPAGEITVDAGATQAILERGSSLLPKGI
HHHHHHHCCCCCEEECCCCCCCCCCCCEEEECCCCCCEEECCCCHHHHHHCCCCCCCCCE
KIVSGNFSRGEVIRIRNSEGRDIAHGVSRYNSDALRLIAGQHSQQIDAILGYEYGPVAVH
EEEECCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHCCCCCCEEEE
RDDMIIR
CCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA