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The map label for this gene is acoA [H]

Identifier: 238893249

GI number: 238893249

Start: 1092278

End: 1093237

Strand: Reverse

Name: acoA [H]

Synonym: KP1_1119

Alternate gene names: 238893249

Gene position: 1093237-1092278 (Counterclockwise)

Preceding gene: 238893251

Following gene: 238893248

Centisome position: 20.83

GC content: 56.35

Gene sequence:

>960_bases
ATGCTCAGCAAACAGGCGTTATTGCAGGCTTACCGCAAGATGCGGGAGATCAGAACATTTGAAGAGCGGTTGCATCAGGA
AAATACCAGCGGTGATATTCCGGGCTTCATTCACCTTTACACCGGTGAGGAGGCCATCGCGGTGGGGGTCTGCGAAAATT
TAACGAGCGCAGATTTTATTGGTTCAACACACCGGGGACATGGCCACTGTATTGCTAAAGGTTGCGACATTCACGGCATG
ATGGCCGAAATATTCGGTAAGGACAGCGGGTTATGTCGCGGTAAGGGCGGGTCGATGCACATTGCCGATCTGTCGAAAGG
AATGCTGGGTGCGAACGCTATCGTTGGGGGAGCCCCTCCCCTGGCCATCGGCGCCGCGCTAACGGCAAAGACGCTAAAAA
CCGGCAACGTCGGTGTCTCTTTTACGGGTGATGGTGGTTCTAATCAGGGCCTGGTCTTTGAAGCCATCAATATGGCCGTC
GTGCTCCAGCTTCCAGCCGTCTTTATTTTCGAGAATAACGGTTACGGCGAAGGGACCGGCCATGACTACGCCGTGGGTGG
GCGTGATATCGCCCGGCGCGCCGCTGGCTTCGGCCTGCCGGCAGTGACCGTCGATGGCACCGATTTCTTTGCCGTTTATG
AGGCAACCTCAGAGGCGGTCAAGCGTGCGCGAGAAGGCGGTGGCCCAAGCGTCATTGAGGCCAAAGCCTTCCGCTGGCAT
GGTCATTTTGAGGGCGATCCCGCGCTATATCGTGCGGAAGGTGAAGTGCAACGCCTGCGTGAACAACATGATCCGCTGAA
GATTTTCACCGCTAAGGTCAAGCAACATATCACCAAGGAAGAACTGGCGGCGATTGACGAGGAAGTAGAAGCCCTGGTCA
ACGATGCCGTATTGAAGGCCCGCGCCGCTGCCTACCCGGCTCCGGAAGACCTGCTGACAGACGTGTACGTCTCATACTGA

Upstream 100 bases:

>100_bases
CCCTTTAACCGCTCGTAGATTCATTGCATGGGTGTGGCCAGGGTTATCCGCCCTGTCTGTTGGCCCCGCCTGGGTGACCT
TTATTGAGGAGAATTAAACG

Downstream 100 bases:

>100_bases
GGGTGATAACCATGACAATAAAAACCTATCGTGAAGCGGTCAAGGAAGCCCTGGCTCAGGAAATGGAACGCGATGAACGC
GTGGTGCTCATCGGTGAAGA

Product: acetoin:DCPIP oxidoreductase alpha subunit

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MLSKQALLQAYRKMREIRTFEERLHQENTSGDIPGFIHLYTGEEAIAVGVCENLTSADFIGSTHRGHGHCIAKGCDIHGM
MAEIFGKDSGLCRGKGGSMHIADLSKGMLGANAIVGGAPPLAIGAALTAKTLKTGNVGVSFTGDGGSNQGLVFEAINMAV
VLQLPAVFIFENNGYGEGTGHDYAVGGRDIARRAAGFGLPAVTVDGTDFFAVYEATSEAVKRAREGGGPSVIEAKAFRWH
GHFEGDPALYRAEGEVQRLREQHDPLKIFTAKVKQHITKEELAAIDEEVEALVNDAVLKARAAAYPAPEDLLTDVYVSY

Sequences:

>Translated_319_residues
MLSKQALLQAYRKMREIRTFEERLHQENTSGDIPGFIHLYTGEEAIAVGVCENLTSADFIGSTHRGHGHCIAKGCDIHGM
MAEIFGKDSGLCRGKGGSMHIADLSKGMLGANAIVGGAPPLAIGAALTAKTLKTGNVGVSFTGDGGSNQGLVFEAINMAV
VLQLPAVFIFENNGYGEGTGHDYAVGGRDIARRAAGFGLPAVTVDGTDFFAVYEATSEAVKRAREGGGPSVIEAKAFRWH
GHFEGDPALYRAEGEVQRLREQHDPLKIFTAKVKQHITKEELAAIDEEVEALVNDAVLKARAAAYPAPEDLLTDVYVSY
>Mature_319_residues
MLSKQALLQAYRKMREIRTFEERLHQENTSGDIPGFIHLYTGEEAIAVGVCENLTSADFIGSTHRGHGHCIAKGCDIHGM
MAEIFGKDSGLCRGKGGSMHIADLSKGMLGANAIVGGAPPLAIGAALTAKTLKTGNVGVSFTGDGGSNQGLVFEAINMAV
VLQLPAVFIFENNGYGEGTGHDYAVGGRDIARRAAGFGLPAVTVDGTDFFAVYEATSEAVKRAREGGGPSVIEAKAFRWH
GHFEGDPALYRAEGEVQRLREQHDPLKIFTAKVKQHITKEELAAIDEEVEALVNDAVLKARAAAYPAPEDLLTDVYVSY

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=321, Percent_Identity=36.7601246105919, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI4505685, Length=321, Percent_Identity=36.7601246105919, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI291084742, Length=321, Percent_Identity=36.7601246105919, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI291084744, Length=328, Percent_Identity=35.9756097560976, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI291084757, Length=321, Percent_Identity=33.3333333333333, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI11386135, Length=322, Percent_Identity=28.2608695652174, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI258645172, Length=322, Percent_Identity=28.2608695652174, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32564172, Length=318, Percent_Identity=38.3647798742138, Blast_Score=208, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17536047, Length=318, Percent_Identity=38.3647798742138, Blast_Score=208, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI86563357, Length=323, Percent_Identity=29.4117647058824, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI86563355, Length=323, Percent_Identity=29.4117647058824, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321026, Length=326, Percent_Identity=37.4233128834356, Blast_Score=193, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24639740, Length=321, Percent_Identity=38.6292834890966, Blast_Score=195, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24639744, Length=321, Percent_Identity=38.6292834890966, Blast_Score=195, Evalue=4e-50,
Organism=Drosophila melanogaster, GI28571106, Length=321, Percent_Identity=38.6292834890966, Blast_Score=195, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24639748, Length=318, Percent_Identity=37.7358490566038, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24639746, Length=308, Percent_Identity=38.3116883116883, Blast_Score=187, Evalue=6e-48,
Organism=Drosophila melanogaster, GI21355903, Length=293, Percent_Identity=28.3276450511945, Blast_Score=101, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 34017; Mature: 34017

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKQALLQAYRKMREIRTFEERLHQENTSGDIPGFIHLYTGEEAIAVGVCENLTSADFI
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCEEEEEHHHCCCCHHHC
GSTHRGHGHCIAKGCDIHGMMAEIFGKDSGLCRGKGGSMHIADLSKGMLGANAIVGGAPP
CCCCCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCCEEHHHHHCCCCCCCCEECCCCC
LAIGAALTAKTLKTGNVGVSFTGDGGSNQGLVFEAINMAVVLQLPAVFIFENNGYGEGTG
HHHHHHHHHHHEECCCEEEEEECCCCCCCCEEEHHHHHEEEEECCEEEEEECCCCCCCCC
HDYAVGGRDIARRAAGFGLPAVTVDGTDFFAVYEATSEAVKRAREGGGPSVIEAKAFRWH
CCEECCCHHHHHHHHCCCCCEEEECCCCEEEEEHHHHHHHHHHHHCCCCCEEEEEEEEEC
GHFEGDPALYRAEGEVQRLREQHDPLKIFTAKVKQHITKEELAAIDEEVEALVNDAVLKA
CCCCCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
RAAAYPAPEDLLTDVYVSY
HHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MLSKQALLQAYRKMREIRTFEERLHQENTSGDIPGFIHLYTGEEAIAVGVCENLTSADFI
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCEEEEEHHHCCCCHHHC
GSTHRGHGHCIAKGCDIHGMMAEIFGKDSGLCRGKGGSMHIADLSKGMLGANAIVGGAPP
CCCCCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCCEEHHHHHCCCCCCCCEECCCCC
LAIGAALTAKTLKTGNVGVSFTGDGGSNQGLVFEAINMAVVLQLPAVFIFENNGYGEGTG
HHHHHHHHHHHEECCCEEEEEECCCCCCCCEEEHHHHHEEEEECCEEEEEECCCCCCCCC
HDYAVGGRDIARRAAGFGLPAVTVDGTDFFAVYEATSEAVKRAREGGGPSVIEAKAFRWH
CCEECCCHHHHHHHHCCCCCEEEECCCCEEEEEHHHHHHHHHHHHCCCCCEEEEEEEEEC
GHFEGDPALYRAEGEVQRLREQHDPLKIFTAKVKQHITKEELAAIDEEVEALVNDAVLKA
CCCCCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
RAAAYPAPEDLLTDVYVSY
HHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286 [H]