Click here to switch to the map view.

The map label for this gene is lhgO [H]

Identifier: 238893234

GI number: 238893234

Start: 1076630

End: 1077898

Strand: Reverse

Name: lhgO [H]

Synonym: KP1_1103

Alternate gene names: 238893234

Gene position: 1077898-1076630 (Counterclockwise)

Preceding gene: 238893235

Following gene: 238893233

Centisome position: 20.54

GC content: 62.88

Gene sequence:

>1269_bases
ATGTATGATTTCGTGATTATCGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAGTTAATTGAGATTTACCCGGACGC
CCGCATTGCGCTGCTGGAAAAAGAGGCCGGCCCGGCCTGCCATCAGACTGGCCACAACAGCGGCGTGATCCACGCCGGGG
TGTATTACACGCCCGGCAGCCTCAAGGCGCAGTTTTGCCTCGCCGGCAACCGCGCCACCAAGGCCTTTTGCGAGCAGAAC
GGCATCCGCTATGACGTCTGCGGCAAAATGCTGGTCGCCACCTCACCGCTGGAGATGGAGCGGATGCGCGCCCTGTGGGA
TCGCACCGCCGCCAACGGCCTGCAGCGCGAGTGGCTAAGCGCCGGGGAGCTACGCGAGCGCGAACCGAACATTACCGGGC
TCGGCGGGATCTTCGTCCCTTCCAGCGGCATCGTCAGCTACCGCGAAGTGGCGGCGGCAATGGCGAAGAACTTCGAAGCG
AAAGGCGGAACGATCGTCTACAACGCCGAAGTCAGCGCCCTGAAAGAACATGCCAGCGGCGTGGTGATCCGCACCCGCCA
GGGCGGAGAATATGAGGCCTCGACGCTGATCGCCTGTTCGGGCTTGATGGCCGATCGGCTGGTAAAAATGCTCGGCGTCG
ACCCCGGGTTTATCATTTGTCCGTTCCGCGGCGAGTATTTCCAGCTGGCGCCGCAGCACAACCAGATCGTCAACCATTTG
ATCTATCCGATCCCCGATCCGGCGATGCCGTTCCTCGGCGTCCACCTGACCCGCATGATCGACGGCAGCGTCACCGTCGG
CCCCAACGCCGTACTGGCTCTGAAGCGCGAAGGCTACCGCAAGCGCGATATCTCGCTGGCCGATACCCTGGAGATCCTCA
CCTCGCCCGGCATCCGCCGGGTACTGCAAAACAACCTGCGCTCCGGACTGGGCGAAATGAAGAATTCGCTGTGCAGAAGC
GGCTATCTGCGGCTGGTGCAAAAATACTGCCCGAGCCTGACGCTGAGCGATTTACGCCCGTGGCCGGCCGGCGTGCGGGC
GCAGGCCGTGTCGCCGCAGGGCAAGCTGATCGACGATTTTCTCTTCGTTACCACCGCGCGTTCGATACATACCTGCAATG
CGCCATCGCCAGCGGCCACCTCCGCTATCCCGATCGGCGCGCATATCGTCAGCAAGGTGCAGAGCCTGCTGGCCAGCCAG
AGCAATCCCGGACGCACGCTGCGTGCGGCACGTAGCGTGGAGACACTACACGCCGCTTTTACCCGTTAA

Upstream 100 bases:

>100_bases
CCTGCGCCGCGAGCTGATGCGCCAGCGCGGCTACTTTGCGTATTCCACGAATCATTACCAGACTCATCAATAAGGGTTTT
CAGGCGAAGGAATTGAGCGG

Downstream 100 bases:

>100_bases
CCCCCCCTGAAGACAGGAAGCAATTATGCAACTCAACGATATGACACTGTTTCGCCAGCAGGCGCTGATCGATGGCCAGT
GGCGCGATGCGCCCAACGGC

Product: hydroxyglutarate oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MYDFVIIGGGIIGMSTAMQLIEIYPDARIALLEKEAGPACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCEQN
GIRYDVCGKMLVATSPLEMERMRALWDRTAANGLQREWLSAGELREREPNITGLGGIFVPSSGIVSYREVAAAMAKNFEA
KGGTIVYNAEVSALKEHASGVVIRTRQGGEYEASTLIACSGLMADRLVKMLGVDPGFIICPFRGEYFQLAPQHNQIVNHL
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLALKREGYRKRDISLADTLEILTSPGIRRVLQNNLRSGLGEMKNSLCRS
GYLRLVQKYCPSLTLSDLRPWPAGVRAQAVSPQGKLIDDFLFVTTARSIHTCNAPSPAATSAIPIGAHIVSKVQSLLASQ
SNPGRTLRAARSVETLHAAFTR

Sequences:

>Translated_422_residues
MYDFVIIGGGIIGMSTAMQLIEIYPDARIALLEKEAGPACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCEQN
GIRYDVCGKMLVATSPLEMERMRALWDRTAANGLQREWLSAGELREREPNITGLGGIFVPSSGIVSYREVAAAMAKNFEA
KGGTIVYNAEVSALKEHASGVVIRTRQGGEYEASTLIACSGLMADRLVKMLGVDPGFIICPFRGEYFQLAPQHNQIVNHL
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLALKREGYRKRDISLADTLEILTSPGIRRVLQNNLRSGLGEMKNSLCRS
GYLRLVQKYCPSLTLSDLRPWPAGVRAQAVSPQGKLIDDFLFVTTARSIHTCNAPSPAATSAIPIGAHIVSKVQSLLASQ
SNPGRTLRAARSVETLHAAFTR
>Mature_422_residues
MYDFVIIGGGIIGMSTAMQLIEIYPDARIALLEKEAGPACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCEQN
GIRYDVCGKMLVATSPLEMERMRALWDRTAANGLQREWLSAGELREREPNITGLGGIFVPSSGIVSYREVAAAMAKNFEA
KGGTIVYNAEVSALKEHASGVVIRTRQGGEYEASTLIACSGLMADRLVKMLGVDPGFIICPFRGEYFQLAPQHNQIVNHL
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLALKREGYRKRDISLADTLEILTSPGIRRVLQNNLRSGLGEMKNSLCRS
GYLRLVQKYCPSLTLSDLRPWPAGVRAQAVSPQGKLIDDFLFVTTARSIHTCNAPSPAATSAIPIGAHIVSKVQSLLASQ
SNPGRTLRAARSVETLHAAFTR

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=398, Percent_Identity=40.7035175879397, Blast_Score=311, Evalue=1e-84,
Organism=Escherichia coli, GI87082148, Length=422, Percent_Identity=88.6255924170616, Blast_Score=771, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17565764, Length=401, Percent_Identity=41.3965087281795, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24585081, Length=407, Percent_Identity=39.0663390663391, Blast_Score=273, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 45664; Mature: 45664

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDFVIIGGGIIGMSTAMQLIEIYPDARIALLEKEAGPACHQTGHNSGVIHAGVYYTPGS
CCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCCEEEEEEEECCCC
LKAQFCLAGNRATKAFCEQNGIRYDVCGKMLVATSPLEMERMRALWDRTAANGLQREWLS
CEEEEEECCCHHHHHHHHHCCCEEHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
AGELREREPNITGLGGIFVPSSGIVSYREVAAAMAKNFEAKGGTIVYNAEVSALKEHASG
CCCHHHCCCCCEECCEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCC
VVIRTRQGGEYEASTLIACSGLMADRLVKMLGVDPGFIICPFRGEYFQLAPQHNQIVNHL
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCHHHHHHHC
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLALKREGYRKRDISLADTLEILTSPGIRR
CCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHHCCHHHH
VLQNNLRSGLGEMKNSLCRSGYLRLVQKYCPSLTLSDLRPWPAGVRAQAVSPQGKLIDDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEEECCCCCCHHHHH
LFVTTARSIHTCNAPSPAATSAIPIGAHIVSKVQSLLASQSNPGRTLRAARSVETLHAAF
HHHHHHCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
TR
CC
>Mature Secondary Structure
MYDFVIIGGGIIGMSTAMQLIEIYPDARIALLEKEAGPACHQTGHNSGVIHAGVYYTPGS
CCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCCEEEEEEEECCCC
LKAQFCLAGNRATKAFCEQNGIRYDVCGKMLVATSPLEMERMRALWDRTAANGLQREWLS
CEEEEEECCCHHHHHHHHHCCCEEHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHC
AGELREREPNITGLGGIFVPSSGIVSYREVAAAMAKNFEAKGGTIVYNAEVSALKEHASG
CCCHHHCCCCCEECCEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHCCC
VVIRTRQGGEYEASTLIACSGLMADRLVKMLGVDPGFIICPFRGEYFQLAPQHNQIVNHL
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCHHHHHHHC
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLALKREGYRKRDISLADTLEILTSPGIRR
CCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHHCCHHHH
VLQNNLRSGLGEMKNSLCRSGYLRLVQKYCPSLTLSDLRPWPAGVRAQAVSPQGKLIDDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEEECCCCCCHHHHH
LFVTTARSIHTCNAPSPAATSAIPIGAHIVSKVQSLLASQSNPGRTLRAARSVETLHAAF
HHHHHHCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
TR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]