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The map label for this gene is pulF [H]

Identifier: 238893137

GI number: 238893137

Start: 965804

End: 967018

Strand: Reverse

Name: pulF [H]

Synonym: KP1_0988

Alternate gene names: 238893137

Gene position: 967018-965804 (Counterclockwise)

Preceding gene: 238893138

Following gene: 238893136

Centisome position: 18.42

GC content: 66.67

Gene sequence:

>1215_bases
ATGGCCCTGTTTCGCTATCAGGCGCTGGACGCGCAGGGCAAAACCCGCCGCGGCCTTCAGCAGGCCGACTCCGCCCGCCA
CGCCCGCCAGCTGCTGCGGGACAAGGGCTGGCTGGCGCTGGAAGTGACAACTGCCGACCCGGCGCGGCGACTGTGGGCCG
GCGGATCCCTTACCCGGCGTACCAGCGCCGGTGATTTAGCCCTGCTCACCCGCCAGCTGGCGACTCTGGTTGCCGCCGGG
ATCCCGCTGGAAAAGGCGCTCGATGCCGTCGCCCAGCAGTGCGAAAAGCCGTCGCTAAGAACGTTAATGGCCGGGGTGCG
CAGCAAAGTGCTCGAAGGCCACTCGCTGGCGGAGGCGATGCGCGCCTATCCCGCCTGCTTCGACGGGCTGTTCTGCGCCA
TGGTGGCGGCCGGCGAAACCTCCGGCCATCTCGACGGCGTACTGAACCGGCTTGCTGACTATACCGAGCAGCGCCAGCAG
CTCCGCGCCCGCCTGCTGCAGGCGATGATCTACCCCATCGTACTGACGCTGGTCGCTATCAGCGTGATCGCCATTCTGCT
CTCCACCGTGGTGCCGAAAGTCGTTGAGCAGTTTGTCCATCTTAAACAGGCCCTGCCCTTCTCTACCCGGCTGCTAATGA
GCCTGAGCGACATCGTGCGCAGCGCGGGCCCCTGGCTGGCGCTGCTGTCGTTACTGGCGCTGCTGGCGCTGCGCTATCTG
CTGCGCCAGCCTGCCCGGCGGCTCGCCTGGGACCGGGCGCTGCTGCGCCTGCCGGTCATCGGCCGCGTGGCCCGCAGCGT
CAACAGCGCGCGCTACGCCCGCACCTTAAGCATTCTCAATGCCAGCGCAGTGCCGCTGCTGCTGTCGATGCGCATCAGCG
CCGACGTCCTCAGCAACGCCTGGGCGCGCAGCCAGCTGGCGGCGGCCAGCGAATCGGTACGCGAAGGCGTTAGTCTGCAT
CGGGCGCTGGAATCCACCGCCCTGTTTCCGCCGATGATGCGCTACATGATCGCCAGCGGCGAACAGAGCGGCGAACTGAC
CGCCATGCTGGAGCGCGCGGCGGAGAACCAGGACCGGGAGCTGAGCGCGCAGATCCAGATGGCCCTCAGCCTGTTTGAGC
CTCTGCTGGTGGTCACCATGGCCGGCATGGTGCTGTTTATCGTGCTGGCCATCCTGCAGCCGATCCTGCAACTGAACACC
CTTATGAGTATGTAA

Upstream 100 bases:

>100_bases
ACCCTGCGCCATGCCGGACGCGACAAAGCGCTGGCGGGGATCACCAGCTGGGAGGAGGTGATGCGGGTCACCGAACAGCA
AACGACGGAAAGCGTCTAAG

Downstream 100 bases:

>100_bases
CTGTATTAATGGAGAAAACTATGCAACGGCAACGCGGTTTCACGTTACTGGAAATTATGGTGGTGATCGTCATTCTCGGC
ATTCTCGCCAGCCTGGTGGT

Product: pullulanase-specific type II secretion system component F

Products: NA

Alternate protein names: Pullulanase secretion protein pulF [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MALFRYQALDAQGKTRRGLQQADSARHARQLLRDKGWLALEVTTADPARRLWAGGSLTRRTSAGDLALLTRQLATLVAAG
IPLEKALDAVAQQCEKPSLRTLMAGVRSKVLEGHSLAEAMRAYPACFDGLFCAMVAAGETSGHLDGVLNRLADYTEQRQQ
LRARLLQAMIYPIVLTLVAISVIAILLSTVVPKVVEQFVHLKQALPFSTRLLMSLSDIVRSAGPWLALLSLLALLALRYL
LRQPARRLAWDRALLRLPVIGRVARSVNSARYARTLSILNASAVPLLLSMRISADVLSNAWARSQLAAASESVREGVSLH
RALESTALFPPMMRYMIASGEQSGELTAMLERAAENQDRELSAQIQMALSLFEPLLVVTMAGMVLFIVLAILQPILQLNT
LMSM

Sequences:

>Translated_404_residues
MALFRYQALDAQGKTRRGLQQADSARHARQLLRDKGWLALEVTTADPARRLWAGGSLTRRTSAGDLALLTRQLATLVAAG
IPLEKALDAVAQQCEKPSLRTLMAGVRSKVLEGHSLAEAMRAYPACFDGLFCAMVAAGETSGHLDGVLNRLADYTEQRQQ
LRARLLQAMIYPIVLTLVAISVIAILLSTVVPKVVEQFVHLKQALPFSTRLLMSLSDIVRSAGPWLALLSLLALLALRYL
LRQPARRLAWDRALLRLPVIGRVARSVNSARYARTLSILNASAVPLLLSMRISADVLSNAWARSQLAAASESVREGVSLH
RALESTALFPPMMRYMIASGEQSGELTAMLERAAENQDRELSAQIQMALSLFEPLLVVTMAGMVLFIVLAILQPILQLNT
LMSM
>Mature_403_residues
ALFRYQALDAQGKTRRGLQQADSARHARQLLRDKGWLALEVTTADPARRLWAGGSLTRRTSAGDLALLTRQLATLVAAGI
PLEKALDAVAQQCEKPSLRTLMAGVRSKVLEGHSLAEAMRAYPACFDGLFCAMVAAGETSGHLDGVLNRLADYTEQRQQL
RARLLQAMIYPIVLTLVAISVIAILLSTVVPKVVEQFVHLKQALPFSTRLLMSLSDIVRSAGPWLALLSLLALLALRYLL
RQPARRLAWDRALLRLPVIGRVARSVNSARYARTLSILNASAVPLLLSMRISADVLSNAWARSQLAAASESVREGVSLHR
ALESTALFPPMMRYMIASGEQSGELTAMLERAAENQDRELSAQIQMALSLFEPLLVVTMAGMVLFIVLAILQPILQLNTL
MSM

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=402, Percent_Identity=46.2686567164179, Blast_Score=355, Evalue=3e-99,
Organism=Escherichia coli, GI1786295, Length=406, Percent_Identity=25.3694581280788, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 44194; Mature: 44063

Theoretical pI: Translated: 10.79; Mature: 10.79

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFRYQALDAQGKTRRGLQQADSARHARQLLRDKGWLALEVTTADPARRLWAGGSLTRR
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCCC
TSAGDLALLTRQLATLVAAGIPLEKALDAVAQQCEKPSLRTLMAGVRSKVLEGHSLAEAM
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHH
RAYPACFDGLFCAMVAAGETSGHLDGVLNRLADYTEQRQQLRARLLQAMIYPIVLTLVAI
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVIAILLSTVVPKVVEQFVHLKQALPFSTRLLMSLSDIVRSAGPWLALLSLLALLALRYL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LRQPARRLAWDRALLRLPVIGRVARSVNSARYARTLSILNASAVPLLLSMRISADVLSNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WARSQLAAASESVREGVSLHRALESTALFPPMMRYMIASGEQSGELTAMLERAAENQDRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHH
LSAQIQMALSLFEPLLVVTMAGMVLFIVLAILQPILQLNTLMSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALFRYQALDAQGKTRRGLQQADSARHARQLLRDKGWLALEVTTADPARRLWAGGSLTRR
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCCC
TSAGDLALLTRQLATLVAAGIPLEKALDAVAQQCEKPSLRTLMAGVRSKVLEGHSLAEAM
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHH
RAYPACFDGLFCAMVAAGETSGHLDGVLNRLADYTEQRQQLRARLLQAMIYPIVLTLVAI
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVIAILLSTVVPKVVEQFVHLKQALPFSTRLLMSLSDIVRSAGPWLALLSLLALLALRYL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LRQPARRLAWDRALLRLPVIGRVARSVNSARYARTLSILNASAVPLLLSMRISADVLSNA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WARSQLAAASESVREGVSLHRALESTALFPPMMRYMIASGEQSGELTAMLERAAENQDRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHH
LSAQIQMALSLFEPLLVVTMAGMVLFIVLAILQPILQLNTLMSM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1738317 [H]