Click here to switch to the map view.

The map label for this gene is lpdA [H]

Identifier: 238893098

GI number: 238893098

Start: 927845

End: 929272

Strand: Direct

Name: lpdA [H]

Synonym: KP1_0944

Alternate gene names: 238893098

Gene position: 927845-929272 (Clockwise)

Preceding gene: 238893097

Following gene: 238893099

Centisome position: 17.68

GC content: 54.55

Gene sequence:

>1428_bases
ATGATGAGTACTGAAATCAAAACTCAGGTCGTGGTACTTGGGGCAGGCCCCGCAGGTTACTCTGCAGCCTTCCGTTGCGC
TGATTTAGGTCTGGAAACCGTCATCGTAGAACGTTACAGCACCCTCGGTGGTGTTTGTCTGAACGTGGGTTGTATCCCTT
CTAAAGCGCTGCTGCACGTGGCAAAAGTTATCGAAGAAGCGAAAGCGCTGGCCGAACACGGCATCGTTTTCGGCGAACCG
AAAACTGACATTGACAAGATCCGCACCTGGAAAGAAAAAGTCATCACTCAGCTGACCGGTGGTCTGGCTGGCATGGCCAA
AGGTCGTAAAGTGAAGGTGGTTAACGGTCTGGGTAAATTTACCGGCGCTAACACCCTGGAAGTGGAAGGCGAAAACGGCA
AAACCGTGATCAACTTCGACAACGCTATCATCGCGGCGGGTTCCCGTCCGATTCAGCTGCCGTTTATCCCGCATGAAGAT
CCGCGCGTATGGGACTCCACCGACGCGCTGGAACTGAAATCTGTACCGAAACGCATGCTGGTGATGGGCGGCGGTATCAT
CGGTCTGGAAATGGGTACCGTATACCATGCGCTGGGTTCAGAGATTGACGTGGTGGAAATGTTCGACCAGGTTATCCCGG
CTGCCGACAAAGACGTGGTGAAAGTCTTCACCAAACGCATCAGCAAGAAATTTAACCTGATGCTGGAAACCAAAGTGACT
GCCGTTGAAGCGAAAGAAGACGGTATTTACGTTTCCATGGAAGGTAAAAAAGCACCGGCGGAAGCGCAGCGTTACGACGC
AGTGCTGGTCGCAATCGGCCGCGTACCGAATGGTAAAAACCTCGATGCAGGTAAAGCTGGCGTGGAAGTTGACGATCGCG
GCTTCATCCGCGTTGACAAACAAATGCGCACCAACGTGCCGCACATCTTTGCTATCGGCGATATCGTCGGTCAGCCGATG
CTGGCGCACAAAGGTGTCCATGAAGGCCACGTTGCCGCAGAAGTTATCTCCGGTCTGAAACACTACTTCGATCCGAAAGT
GATCCCATCCATCGCCTACACTGAACCAGAAGTGGCATGGGTCGGTCTGACCGAGAAAGAAGCGAAAGAGAAAGGCATCA
GCTACGAAACCGCCACCTTCCCGTGGGCTGCTTCCGGCCGTGCTATCGCTTCTGACTGCGCAGATGGTATGACCAAACTG
ATCTTCGACAAAGAGACCCACCGTGTTATCGGCGGCGCGATTGTCGGCACCAACGGCGGCGAGCTGCTGGGTGAGATCGG
CCTGGCTATCGAGATGGGCTGTGACGCTGAAGACATCGCCCTGACCATCCACGCTCACCCGACTCTGCACGAGTCCGTTG
GCCTGGCGGCGGAAGTGTTCGAAGGCAGCATCACCGACCTGCCAAACGCCAAAGCGAAGAAAAAGTAA

Upstream 100 bases:

>100_bases
TTGTTAACAATTTTGTAAAATACACGCGGATAGAACGTCCCGGTGGACTTGGGCGTAAGAATAATAGCGTCAGACCCGCC
GGATATAAATTACAGAGGTC

Downstream 100 bases:

>100_bases
CTTTTTCTTTCAGGAAAAAAGCATAAGCGGCTCCGGGAGCCGCTTTTTTTATGCCTGATGTTTAGAACTATGTCACTGTT
CATAAACCGCTACACCTCAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MMSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEP
KTDIDKIRTWKEKVITQLTGGLAGMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHED
PRVWDSTDALELKSVPKRMLVMGGGIIGLEMGTVYHALGSEIDVVEMFDQVIPAADKDVVKVFTKRISKKFNLMLETKVT
AVEAKEDGIYVSMEGKKAPAEAQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDKQMRTNVPHIFAIGDIVGQPM
LAHKGVHEGHVAAEVISGLKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKL
IFDKETHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNAKAKKK

Sequences:

>Translated_475_residues
MMSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEP
KTDIDKIRTWKEKVITQLTGGLAGMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHED
PRVWDSTDALELKSVPKRMLVMGGGIIGLEMGTVYHALGSEIDVVEMFDQVIPAADKDVVKVFTKRISKKFNLMLETKVT
AVEAKEDGIYVSMEGKKAPAEAQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDKQMRTNVPHIFAIGDIVGQPM
LAHKGVHEGHVAAEVISGLKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKL
IFDKETHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNAKAKKK
>Mature_475_residues
MMSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEP
KTDIDKIRTWKEKVITQLTGGLAGMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHED
PRVWDSTDALELKSVPKRMLVMGGGIIGLEMGTVYHALGSEIDVVEMFDQVIPAADKDVVKVFTKRISKKFNLMLETKVT
AVEAKEDGIYVSMEGKKAPAEAQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDKQMRTNVPHIFAIGDIVGQPM
LAHKGVHEGHVAAEVISGLKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKL
IFDKETHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNAKAKKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=454, Percent_Identity=43.3920704845815, Blast_Score=344, Evalue=8e-95,
Organism=Homo sapiens, GI50301238, Length=453, Percent_Identity=28.0353200883002, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI22035672, Length=429, Percent_Identity=28.9044289044289, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI148277065, Length=437, Percent_Identity=27.4599542334096, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI33519430, Length=437, Percent_Identity=27.4599542334096, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI33519428, Length=437, Percent_Identity=27.4599542334096, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI33519426, Length=437, Percent_Identity=27.4599542334096, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI148277071, Length=437, Percent_Identity=27.4599542334096, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI291045266, Length=435, Percent_Identity=27.5862068965517, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI291045268, Length=429, Percent_Identity=26.1072261072261, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1786307, Length=474, Percent_Identity=96.8354430379747, Blast_Score=934, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=469, Percent_Identity=27.0788912579957, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=27.2527472527473, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1789915, Length=444, Percent_Identity=28.1531531531532, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI32565766, Length=448, Percent_Identity=40.1785714285714, Blast_Score=324, Evalue=6e-89,
Organism=Caenorhabditis elegans, GI71983429, Length=436, Percent_Identity=26.8348623853211, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71983419, Length=436, Percent_Identity=26.8348623853211, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17557007, Length=492, Percent_Identity=27.0325203252033, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=441, Percent_Identity=26.3038548752834, Blast_Score=119, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=40.7894736842105, Blast_Score=307, Evalue=2e-84,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=26.6524520255864, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=471, Percent_Identity=27.3885350318471, Blast_Score=150, Evalue=5e-37,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=40.2625820568928, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24640549, Length=462, Percent_Identity=29.4372294372294, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640553, Length=462, Percent_Identity=29.4372294372294, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640551, Length=462, Percent_Identity=29.4372294372294, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI17737741, Length=479, Percent_Identity=26.3048016701461, Blast_Score=115, Evalue=9e-26,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50695; Mature: 50695

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHV
CCCCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHH
AKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVITQLTGGLAGMAKGRKVKVVNGLGKF
HHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCHHC
TGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDPRVWDSTDALELKSVPKRML
CCCCEEEEECCCCCEEEEECCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHCCHHHH
VMGGGIIGLEMGTVYHALGSEIDVVEMFDQVIPAADKDVVKVFTKRISKKFNLMLETKVT
HCCCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEE
AVEAKEDGIYVSMEGKKAPAEAQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDK
EEEECCCCEEEEECCCCCCCHHHHHCEEEEEEECCCCCCCCCCCCCCCEECCCCEEEECH
QMRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVISGLKHYFDPKVIPSIAYTEPEVAW
HHHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEE
VGLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKLIFDKETHRVIGGAIVGTNGG
EECCHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHEEECEEEEECCCH
ELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNAKAKKK
HHHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGVCLNVGCIPSKALLHV
CCCCCCEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHH
AKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVITQLTGGLAGMAKGRKVKVVNGLGKF
HHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCHHC
TGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDPRVWDSTDALELKSVPKRML
CCCCEEEEECCCCCEEEEECCEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHCCHHHH
VMGGGIIGLEMGTVYHALGSEIDVVEMFDQVIPAADKDVVKVFTKRISKKFNLMLETKVT
HCCCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEE
AVEAKEDGIYVSMEGKKAPAEAQRYDAVLVAIGRVPNGKNLDAGKAGVEVDDRGFIRVDK
EEEECCCCEEEEECCCCCCCHHHHHCEEEEEEECCCCCCCCCCCCCCCEECCCCEEEECH
QMRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVISGLKHYFDPKVIPSIAYTEPEVAW
HHHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEE
VGLTEKEAKEKGISYETATFPWAASGRAIASDCADGMTKLIFDKETHRVIGGAIVGTNGG
EECCHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHEEECEEEEECCCH
ELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEVFEGSITDLPNAKAKKK
HHHHHCCEEEEECCCCCCEEEEEECCCCHHHHHCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]