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The map label for this gene is aceF [H]

Identifier: 238893097

GI number: 238893097

Start: 925743

End: 927641

Strand: Direct

Name: aceF [H]

Synonym: KP1_0943

Alternate gene names: 238893097

Gene position: 925743-927641 (Clockwise)

Preceding gene: 238893096

Following gene: 238893098

Centisome position: 17.64

GC content: 57.87

Gene sequence:

>1899_bases
ATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTTGGCGACAA
AGTAGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTCCCGTCTCCGCAGGCTGGCGTCG
TGAAAGAGATTAAAGTCTCCGTCGGCGACAAAACCGAGACTGGCAAACTGATTATGATTTTCGATTCCGCCGAAGGTGCA
GCCGCCGCTGCACCTGCGCAGGAAGAGAAGAAAGAAGCCGCTCCGGCCGCCGCTGCTCCAGCGGCTGCCGCGGCAGCGAA
AGAAGTTCACGTCCCGGATATCGGCGGCGATGAAGTCGAAGTCACTGAGATCATGGTTAAAGTGGGCGACACCATCGCCG
CTGAGCAATCCCTGATCACCGTAGAAGGCGATAAAGCCTCTATGGAAGTTCCGGCGCCGTTCGCCGGTACCGTCAAAGAG
ATCAAAATCAATACCGGCGATAAAGTTTCCACCGGCTCCCTGATTATGATCTTCGAAGTTGCGGGCGCTGCGCCTGCAGC
CGCTCCGGCACAGGCTGCCGCGCCGGCTGCTGCGGCTCCGGCTGCCGCAGCAGGCGTGAAAGATGTTAACGTCCCGGACA
TCGGCGGCGACGAAGTTGAAGTCACCGAAGTGATGGTTAAAGTCGGCGATAAAGTCGCGGCGGAACAGTCCCTGATCACC
GTAGAGGGCGACAAAGCCTCTATGGAAGTTCCGGCGCCGTTCGCGGGTACCGTTAAAGAGATCAAAATCAGCACCGGCGA
TAAAGTCAAAACCGGTTCCCTGATCATGGTCTTCGAAGTGGAAGGCGCTGCGCCTGCCGCCGCTCCGGCTCAGGCTGCTG
CACCGGCACCAGCTGCTGCCCCGGCTCAGGCCGCTAAGCCTGCCGCTGCGCCGGCTGCGAAAGCAGAAGGTAAAAGCGAA
TTCGCTGAAAACGATGCCTACGTTCACGCGACCCCGCTGATTCGCCGCCTGGCGCGCGAGTTCGGTGTTAACCTGGCGAA
AGTGAAAGGCACCGGCCGTAAAGGTCGTATCCTGCGCGAAGACGTTCAGGCCTACGTGAAAGACGCGGTTAAACGCGCTG
AATCCGCACCGGCGGCTGCCGCTGGCGGCGGTATCCCGGGCATGCTGCCGTGGCCGAAGGTTGACTTCAGCAAGTTTGGC
GAAGTCGAAGAAGTGGAGCTGGGTCGTATCCAGAAAATCTCTGGCGCCAACCTGAGCCGTAACTGGGTGATGATCCCGCA
CGTTACCCACTTCGACAAAACCGATATCACCGATCTGGAAGCGTTCCGCAAGCAGCAGAATGCCGAAGCTGAGAAGCGTA
AACTGGACGTGAAATTCACTCCAGTGGTCTTCATCATGAAAGCGGTTGCCGCTGCGCTTGAGCAGATGCCGCGCTTCAAC
AGCTCGCTCTCCGAAGATGGTCAGCGCCTGACGCTGAAGAAATACATCAACATCGGTGTGGCGGTGGATACCCCGAACGG
TCTGGTGGTTCCGGTCTTCAAAGACGTGAACAAGAAGAGCATCACCGAGCTGTCTCGTGAATTGACCACCATCTCGAAGA
AAGCGCGCGATGGCAAACTGACGGCTGGCGAAATGCAGGGCGGTTGCTTCACCATCTCCAGCATTGGCGGCCTGGGTACC
ACCCACTTCGCGCCGATTGTTAACGCGCCGGAAGTGGCCATCCTCGGCGTGTCTAAATCCGCGATGGAGCCGGTATGGAA
TGGTAAAGAGTTTGTGCCGCGCCTGATGCTGCCGATCTCTCTGTCCTTCGACCACCGCGTCATCGACGGTGCTGATGGTG
CCCGCTTCATTACCATCATTAACAACACCCTGAGCGACATTCGCCGCCTGGTGATGTAA

Upstream 100 bases:

>100_bases
GTGGTGAAATCGATAAGAAAGTGGTGGCTGACGCTATCGCGAAATTCGACATCGATGCAGAAAAAGTTAACCCGCGTCTG
GCGTAAGAGGTAAAAGAATA

Downstream 100 bases:

>100_bases
TCGAAAAAGCCGGCCTGACGGCCGGCTTTTTTCTGATAACCTCAGGCTGTTGGGGATTATCAGCAACAAAGGACAAAATC
GTTTGCCGTTTGTTGTTTAA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVSVGDKTETGKLIMIFDSAEGA
AAAAPAQEEKKEAAPAAAAPAAAAAAKEVHVPDIGGDEVEVTEIMVKVGDTIAAEQSLITVEGDKASMEVPAPFAGTVKE
IKINTGDKVSTGSLIMIFEVAGAAPAAAPAQAAAPAAAAPAAAAGVKDVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLIT
VEGDKASMEVPAPFAGTVKEIKISTGDKVKTGSLIMVFEVEGAAPAAAPAQAAAPAPAAAPAQAAKPAAAPAAKAEGKSE
FAENDAYVHATPLIRRLAREFGVNLAKVKGTGRKGRILREDVQAYVKDAVKRAESAPAAAAGGGIPGMLPWPKVDFSKFG
EVEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITDLEAFRKQQNAEAEKRKLDVKFTPVVFIMKAVAAALEQMPRFN
SSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKSITELSRELTTISKKARDGKLTAGEMQGGCFTISSIGGLGT
THFAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRLVM

Sequences:

>Translated_632_residues
MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVSVGDKTETGKLIMIFDSAEGA
AAAAPAQEEKKEAAPAAAAPAAAAAAKEVHVPDIGGDEVEVTEIMVKVGDTIAAEQSLITVEGDKASMEVPAPFAGTVKE
IKINTGDKVSTGSLIMIFEVAGAAPAAAPAQAAAPAAAAPAAAAGVKDVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLIT
VEGDKASMEVPAPFAGTVKEIKISTGDKVKTGSLIMVFEVEGAAPAAAPAQAAAPAPAAAPAQAAKPAAAPAAKAEGKSE
FAENDAYVHATPLIRRLAREFGVNLAKVKGTGRKGRILREDVQAYVKDAVKRAESAPAAAAGGGIPGMLPWPKVDFSKFG
EVEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITDLEAFRKQQNAEAEKRKLDVKFTPVVFIMKAVAAALEQMPRFN
SSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKSITELSRELTTISKKARDGKLTAGEMQGGCFTISSIGGLGT
THFAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRLVM
>Mature_631_residues
AIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVSVGDKTETGKLIMIFDSAEGAA
AAAPAQEEKKEAAPAAAAPAAAAAAKEVHVPDIGGDEVEVTEIMVKVGDTIAAEQSLITVEGDKASMEVPAPFAGTVKEI
KINTGDKVSTGSLIMIFEVAGAAPAAAPAQAAAPAAAAPAAAAGVKDVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLITV
EGDKASMEVPAPFAGTVKEIKISTGDKVKTGSLIMVFEVEGAAPAAAPAQAAAPAPAAAPAQAAKPAAAPAAKAEGKSEF
AENDAYVHATPLIRRLAREFGVNLAKVKGTGRKGRILREDVQAYVKDAVKRAESAPAAAAGGGIPGMLPWPKVDFSKFGE
VEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITDLEAFRKQQNAEAEKRKLDVKFTPVVFIMKAVAAALEQMPRFNS
SLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKSITELSRELTTISKKARDGKLTAGEMQGGCFTISSIGGLGTT
HFAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRLVM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=425, Percent_Identity=29.6470588235294, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI31711992, Length=406, Percent_Identity=30.7881773399015, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI19923748, Length=205, Percent_Identity=34.1463414634146, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI203098816, Length=446, Percent_Identity=27.1300448430493, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI203098753, Length=446, Percent_Identity=27.3542600896861, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI260898739, Length=169, Percent_Identity=35.5029585798817, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI1786305, Length=633, Percent_Identity=87.9936808846761, Blast_Score=994, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=408, Percent_Identity=29.9019607843137, Blast_Score=165, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=406, Percent_Identity=30.0492610837438, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17560088, Length=429, Percent_Identity=28.4382284382284, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI25146366, Length=207, Percent_Identity=36.231884057971, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17538894, Length=310, Percent_Identity=28.0645161290323, Blast_Score=97, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320352, Length=415, Percent_Identity=27.710843373494, Blast_Score=156, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324258, Length=430, Percent_Identity=29.0697674418605, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI18859875, Length=438, Percent_Identity=30.1369863013699, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24645909, Length=215, Percent_Identity=34.4186046511628, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24582497, Length=235, Percent_Identity=29.7872340425532, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI20129315, Length=235, Percent_Identity=29.7872340425532, Blast_Score=112, Evalue=6e-25,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 65902; Mature: 65771

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVS
CEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEE
VGDKTETGKLIMIFDSAEGAAAAAPAQEEKKEAAPAAAAPAAAAAAKEVHVPDIGGDEVE
CCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCEE
VTEIMVKVGDTIAAEQSLITVEGDKASMEVPAPFAGTVKEIKINTGDKVSTGSLIMIFEV
HHHHHHHHCCCHHCCCEEEEEECCCCCEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEE
AGAAPAAAPAQAAAPAAAAPAAAAGVKDVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLIT
CCCCCCCCCHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCEEEE
VEGDKASMEVPAPFAGTVKEIKISTGDKVKTGSLIMVFEVEGAAPAAAPAQAAAPAPAAA
EECCCCCEECCCCCCCCEEEEEECCCCCEECCCEEEEEEECCCCCCCCCCHHCCCCCCCC
PAQAAKPAAAPAAKAEGKSEFAENDAYVHATPLIRRLAREFGVNLAKVKGTGRKGRILRE
CCHHCCCCCCCCHHCCCCHHHHCCCCEEEHHHHHHHHHHHHCCEEEEEECCCCCCCHHHH
DVQAYVKDAVKRAESAPAAAAGGGIPGMLPWPKVDFSKFGEVEEVELGRIQKISGANLSR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCEECCCCEEEECCCCCCC
NWVMIPHVTHFDKTDITDLEAFRKQQNAEAEKRKLDVKFTPVVFIMKAVAAALEQMPRFN
CEEEECCEECCCCCCCHHHHHHHHHHCCCHHHHEECEEEHHHHHHHHHHHHHHHHCCCCC
SSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKSITELSRELTTISKKARDGKL
CCHHCCCCEEEEEHEEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCC
TAGEMQGGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMLPIS
EECCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCHHHHHHEEEEEE
LSFDHRVIDGADGARFITIINNTLSDIRRLVM
ECCCCEEEECCCCCEEEEEEHHHHHHHHHHHC
>Mature Secondary Structure 
AIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVS
EEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEE
VGDKTETGKLIMIFDSAEGAAAAAPAQEEKKEAAPAAAAPAAAAAAKEVHVPDIGGDEVE
CCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCEE
VTEIMVKVGDTIAAEQSLITVEGDKASMEVPAPFAGTVKEIKINTGDKVSTGSLIMIFEV
HHHHHHHHCCCHHCCCEEEEEECCCCCEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEE
AGAAPAAAPAQAAAPAAAAPAAAAGVKDVNVPDIGGDEVEVTEVMVKVGDKVAAEQSLIT
CCCCCCCCCHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCEEEE
VEGDKASMEVPAPFAGTVKEIKISTGDKVKTGSLIMVFEVEGAAPAAAPAQAAAPAPAAA
EECCCCCEECCCCCCCCEEEEEECCCCCEECCCEEEEEEECCCCCCCCCCHHCCCCCCCC
PAQAAKPAAAPAAKAEGKSEFAENDAYVHATPLIRRLAREFGVNLAKVKGTGRKGRILRE
CCHHCCCCCCCCHHCCCCHHHHCCCCEEEHHHHHHHHHHHHCCEEEEEECCCCCCCHHHH
DVQAYVKDAVKRAESAPAAAAGGGIPGMLPWPKVDFSKFGEVEEVELGRIQKISGANLSR
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCEECCCCEEEECCCCCCC
NWVMIPHVTHFDKTDITDLEAFRKQQNAEAEKRKLDVKFTPVVFIMKAVAAALEQMPRFN
CEEEECCEECCCCCCCHHHHHHHHHHCCCHHHHEECEEEHHHHHHHHHHHHHHHHCCCCC
SSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFKDVNKKSITELSRELTTISKKARDGKL
CCHHCCCCEEEEEHEEEEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCC
TAGEMQGGCFTISSIGGLGTTHFAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMLPIS
EECCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCCHHHHHHEEEEEE
LSFDHRVIDGADGARFITIINNTLSDIRRLVM
ECCCCEEEECCCCCEEEEEEHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]