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The map label for this gene is yabF

Identifier: 238893019

GI number: 238893019

Start: 832635

End: 833168

Strand: Direct

Name: yabF

Synonym: KP1_0856

Alternate gene names: 238893019

Gene position: 832635-833168 (Clockwise)

Preceding gene: 238893018

Following gene: 238893020

Centisome position: 15.86

GC content: 57.12

Gene sequence:

>534_bases
ATGATTCTGATAATTTATGCGCATCCTTATCCGCAGCATTCTCATGCGAATAAGCGAATGCTTGAACAGGCAGGGACGCT
TGAAGGCGTAGAGATACGCTCCCTCTATCAACTCTATCCCGACTTTAATATCGATGTCGCCGCCGAGCAGGCGGCGCTGG
CCCGTGCCGATCTGGTTATCTGGCAGCATCCTATGCAGTGGTATAGCGTACCGCCGCTGCTCAAGCTGTGGATGGACAAG
GTGCTGTCGCACGGCTGGGCCTATGGTCACAACGGTATTGCCCTGCGCGGTAAATCGCTGATGTGGGCGGTGACCACCGG
CGGCGGTGAAAGCCACTTCGACATCGGCTCATTCCCCGGTTTTCCGGTGCTGGCGCAGCCGTTGCAGGCAACCGCGCTTT
ACTGCGGAATGAAATGGCTGCCGCCGTTCGCTATGCACTGCACCTTTATCTGCGATGATGAAACGCTGCAGGCGCAGGCC
CGCCGCTATCGTCAACGCTTAATCGACTGGCAGGAGGCACACCAGAATGGATAG

Upstream 100 bases:

>100_bases
CGTAAGCCCTCGCGACATAAATCACAGTTATCATTGTAAGCGGTTCGCTTAACTGTCTACACTCTTGCTGATTACGACAA
GAGCAAACGCAGGCCAATCA

Downstream 100 bases:

>100_bases
CCATACGCTGATACAGGCGTTGATTTACCTCGGCGCCGCGGCGCTGATTGTGCCCATCGCCGTCCGTCTGGGGCTGGGGT
CAGTGCTGGGCTATCTCATC

Product: glutathione-regulated potassium-efflux system ancillary protein KefF

Products: NAD(P); reduced acceptor [C]

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MILIIYAHPYPQHSHANKRMLEQAGTLEGVEIRSLYQLYPDFNIDVAAEQAALARADLVIWQHPMQWYSVPPLLKLWMDK
VLSHGWAYGHNGIALRGKSLMWAVTTGGGESHFDIGSFPGFPVLAQPLQATALYCGMKWLPPFAMHCTFICDDETLQAQA
RRYRQRLIDWQEAHQNG

Sequences:

>Translated_177_residues
MILIIYAHPYPQHSHANKRMLEQAGTLEGVEIRSLYQLYPDFNIDVAAEQAALARADLVIWQHPMQWYSVPPLLKLWMDK
VLSHGWAYGHNGIALRGKSLMWAVTTGGGESHFDIGSFPGFPVLAQPLQATALYCGMKWLPPFAMHCTFICDDETLQAQA
RRYRQRLIDWQEAHQNG
>Mature_177_residues
MILIIYAHPYPQHSHANKRMLEQAGTLEGVEIRSLYQLYPDFNIDVAAEQAALARADLVIWQHPMQWYSVPPLLKLWMDK
VLSHGWAYGHNGIALRGKSLMWAVTTGGGESHFDIGSFPGFPVLAQPLQATALYCGMKWLPPFAMHCTFICDDETLQAQA
RRYRQRLIDWQEAHQNG

Specific function: Required for full activity of kefC

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily

Homologues:

Organism=Homo sapiens, GI156564357, Length=151, Percent_Identity=35.7615894039735, Blast_Score=74, Evalue=5e-14,
Organism=Homo sapiens, GI4505415, Length=104, Percent_Identity=36.5384615384615, Blast_Score=68, Evalue=4e-12,
Organism=Escherichia coli, GI1786231, Length=176, Percent_Identity=82.3863636363636, Blast_Score=319, Evalue=6e-89,
Organism=Escherichia coli, GI1789750, Length=164, Percent_Identity=38.4146341463415, Blast_Score=114, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KEFF_KLEAE (Q9X755)

Other databases:

- EMBL:   AJ242913
- ProteinModelPortal:   Q9X755
- SMR:   Q9X755
- HAMAP:   MF_01414
- InterPro:   IPR003680

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 1.6.99.- [C]

Molecular weight: Translated: 20161; Mature: 20161

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILIIYAHPYPQHSHANKRMLEQAGTLEGVEIRSLYQLYPDFNIDVAAEQAALARADLVI
CEEEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHE
WQHPMQWYSVPPLLKLWMDKVLSHGWAYGHNGIALRGKSLMWAVTTGGGESHFDIGSFPG
EECCHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEEEEECCCCCCCCCCCCCC
FPVLAQPLQATALYCGMKWLPPFAMHCTFICDDETLQAQARRYRQRLIDWQEAHQNG
CHHHHHHHHHHHHHHCCCCCCCHHHEEEEEECCHHHHHHHHHHHHHHCCHHHHCCCC
>Mature Secondary Structure
MILIIYAHPYPQHSHANKRMLEQAGTLEGVEIRSLYQLYPDFNIDVAAEQAALARADLVI
CEEEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHE
WQHPMQWYSVPPLLKLWMDKVLSHGWAYGHNGIALRGKSLMWAVTTGGGESHFDIGSFPG
EECCHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCEEEEEEEECCCCCCCCCCCCCC
FPVLAQPLQATALYCGMKWLPPFAMHCTFICDDETLQAQARRYRQRLIDWQEAHQNG
CHHHHHHHHHHHHHHCCCCCCCHHHEEEEEECCHHHHHHHHHHHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; acceptor [C]

Specific reaction: NAD(P)H2 + acceptor = NAD(P) + reduced acceptor [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11053405