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The map label for this gene is glnG [H]

Identifier: 238892281

GI number: 238892281

Start: 32335

End: 33744

Strand: Reverse

Name: glnG [H]

Synonym: KP1_0028

Alternate gene names: 238892281

Gene position: 33744-32335 (Counterclockwise)

Preceding gene: 238892282

Following gene: 238892278

Centisome position: 0.64

GC content: 58.72

Gene sequence:

>1410_bases
ATGCAACGAGGGATAGCCTGGATCGTTGATGACGATAGCTCCATCCGCTGGGTGCTTGAACGCGCGCTCACCGGAGCCGG
CTTGAGCTGCACAACGTTCGAAAGCGGCAATGAGGTGCTAGATGCCCTCACCACCAAAACCCCGGATGTACTGCTGTCAG
ATATCCGTATGCCCGGAATGGATGGTCTGGCGCTGCTCAAACAGATTAAGCAGCGCCATCCAATGCTTCCGGTCATCATA
ATGACCGCGCATTCCGATCTGGACGCCGCGGTCAGCGCTTATCAGCAGGGCGCGTTTGATTATTTGCCCAAACCTTTTGA
TATCGATGAAGCCGTCGCCCTCGTCGACCGGGCGATAAGCCACTATCAAGAGCAGCAGCAGCCGCGAAATGCGCCGATAA
GTAGCCCAACTGCCGACATCATCGGCGAAGCGCCAGCTATGCAGGATGTCTTTCGCATTATTGGCCGCCTGTCGCGCTCA
TCCATCAGCGTGCTGATCAATGGCGAATCCGGTACCGGTAAAGAGCTTGTCGCACATGCCCTGCATCGCCATAGTCCACG
CTCAAAAGCGCCGTTTATCGCGCTGAATATGGCGGCGATCCCCAAGGACCTGATTGAGTCCGAGCTGTTCGGCCATGAAA
AAGGAGCCTTTACCGGCGCAAATACCGTCCGTCAGGGTCGCTTTGAACAGGCTGACGGCGGTACGCTGTTCCTGGATGAA
ATTGGCGATATGCCGCTTGATGTCCAGACTCGTCTGCTGCGCGTGCTGGCGGATGGTCAGTTTTATCGCGTGGGCGGTTA
CGCGCCGGTCAAGGTCGATGTGCGGATCATCGCCGCTACCCACCAGAACCTGGAACAGCGCGTGCAGGAGGGGAAATTTC
GTGAAGATTTGTTCCACCGCCTGAACGTCATCCGGGTGCATTTACCACCGCTGCGTGAGCGCCGGGAAGATATTCCACGC
CTGGCCCGCCATTTTCTGCAGATAGCCGCCCGCGAGCTCGGCGTTGAAGCCAAACAGCTGCATCCGGAAACGGAGACAGC
GCTGACCCGCTTGGCGTGGCCAGGCAACGTCCGTCAGCTGGAAAACACCTGTCGCTGGCTCACCGTCATGGCCGCCGGCC
AGGAGGTGCTGACGCAGGATCTGCCGAGCGAACTGTTTGAGACCACGATTCCTGATAGCCCAACGCAGATGCAGCCTGAC
AGCTGGGCGACGCTGCTGGGTCAGTGGGCCGATCGGGCGTTGCGATCCGGTCATCAAAACCTGCTCTCAGAAGCGCAACC
CGAAATGGAGCGCACGCTGCTAACGACCGCCCTGCGCCATACTCAGGGGCACAAACAGGAGGCTGCGCGCCTGCTGGGAT
GGGGACGTAATACCCTGACGCGTAAGCTAAAAGAGTTGGGTATGGAGTAG

Upstream 100 bases:

>100_bases
GTAGCCTTATCGATCAGCACTCCGGCAAAATTGAATTCACCAGCTGGCCGGGTCATACCGAATTTTCGGTATACCTGCCT
ATTCGGAAGTAGAGGTGTTT

Downstream 100 bases:

>100_bases
GGTTCAACAGCATGATGAAAAATAGCCGATCGGCGCAAAGTGCTGCTATTTTGTACTTTACTGTTCCGATGAGTTGAGTA
TGATCGTGCCCGGCATTAGG

Product: nitrogen regulation protein NR(I)

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MQRGIAWIVDDDSSIRWVLERALTGAGLSCTTFESGNEVLDALTTKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVDRAISHYQEQQQPRNAPISSPTADIIGEAPAMQDVFRIIGRLSRS
SISVLINGESGTGKELVAHALHRHSPRSKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTVRQGRFEQADGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPR
LARHFLQIAARELGVEAKQLHPETETALTRLAWPGNVRQLENTCRWLTVMAAGQEVLTQDLPSELFETTIPDSPTQMQPD
SWATLLGQWADRALRSGHQNLLSEAQPEMERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME

Sequences:

>Translated_469_residues
MQRGIAWIVDDDSSIRWVLERALTGAGLSCTTFESGNEVLDALTTKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVDRAISHYQEQQQPRNAPISSPTADIIGEAPAMQDVFRIIGRLSRS
SISVLINGESGTGKELVAHALHRHSPRSKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTVRQGRFEQADGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPR
LARHFLQIAARELGVEAKQLHPETETALTRLAWPGNVRQLENTCRWLTVMAAGQEVLTQDLPSELFETTIPDSPTQMQPD
SWATLLGQWADRALRSGHQNLLSEAQPEMERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
>Mature_469_residues
MQRGIAWIVDDDSSIRWVLERALTGAGLSCTTFESGNEVLDALTTKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVII
MTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVDRAISHYQEQQQPRNAPISSPTADIIGEAPAMQDVFRIIGRLSRS
SISVLINGESGTGKELVAHALHRHSPRSKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTVRQGRFEQADGGTLFLDE
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPR
LARHFLQIAARELGVEAKQLHPETETALTRLAWPGNVRQLENTCRWLTVMAAGQEVLTQDLPSELFETTIPDSPTQMQPD
SWATLLGQWADRALRSGHQNLLSEAQPEMERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=469, Percent_Identity=93.3901918976546, Blast_Score=908, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=466, Percent_Identity=42.4892703862661, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1790437, Length=458, Percent_Identity=43.2314410480349, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI1788905, Length=462, Percent_Identity=38.5281385281385, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI87082117, Length=364, Percent_Identity=42.032967032967, Blast_Score=254, Evalue=6e-69,
Organism=Escherichia coli, GI1789233, Length=339, Percent_Identity=43.0678466076696, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1789087, Length=340, Percent_Identity=41.4705882352941, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1786524, Length=339, Percent_Identity=42.7728613569322, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1787583, Length=334, Percent_Identity=40.4191616766467, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI87082152, Length=334, Percent_Identity=40.1197604790419, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI87081872, Length=332, Percent_Identity=39.7590361445783, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI87081858, Length=414, Percent_Identity=27.7777777777778, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789828, Length=316, Percent_Identity=31.6455696202532, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1790860, Length=128, Percent_Identity=33.59375, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789809, Length=121, Percent_Identity=30.5785123966942, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786911, Length=121, Percent_Identity=32.2314049586777, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789402, Length=106, Percent_Identity=33.0188679245283, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52336; Mature: 52336

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRGIAWIVDDDSSIRWVLERALTGAGLSCTTFESGNEVLDALTTKTPDVLLSDIRMPGM
CCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHCCCCCC
DGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVDRAIS
CHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHH
HYQEQQQPRNAPISSPTADIIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELVAHA
HHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
LHRHSPRSKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTVRQGRFEQADGGTLFLDE
HHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCEEEHHH
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDLFHR
HCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEHHHHHHHHHHHHCCHHHHHHHH
LNVIRVHLPPLRERREDIPRLARHFLQIAARELGVEAKQLHPETETALTRLAWPGNVRQL
HHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCCHHHH
ENTCRWLTVMAAGQEVLTQDLPSELFETTIPDSPTQMQPDSWATLLGQWADRALRSGHQN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLSEAQPEMERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
HHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MQRGIAWIVDDDSSIRWVLERALTGAGLSCTTFESGNEVLDALTTKTPDVLLSDIRMPGM
CCCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCHHHHHHHCCCCCC
DGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVDRAIS
CHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHH
HYQEQQQPRNAPISSPTADIIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELVAHA
HHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
LHRHSPRSKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTVRQGRFEQADGGTLFLDE
HHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCEEEHHH
IGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDLFHR
HCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEHHHHHHHHHHHHCCHHHHHHHH
LNVIRVHLPPLRERREDIPRLARHFLQIAARELGVEAKQLHPETETALTRLAWPGNVRQL
HHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCCHHHH
ENTCRWLTVMAAGQEVLTQDLPSELFETTIPDSPTQMQPDSWATLLGQWADRALRSGHQN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLSEAQPEMERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
HHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]