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The map label for this gene is dsbA [H]

Identifier: 238892275

GI number: 238892275

Start: 23687

End: 24310

Strand: Direct

Name: dsbA [H]

Synonym: KP1_0022

Alternate gene names: 238892275

Gene position: 23687-24310 (Clockwise)

Preceding gene: 238892274

Following gene: 238892277

Centisome position: 0.45

GC content: 52.72

Gene sequence:

>624_bases
ATGAAAAAAGTTTGGCTGGCGCTGGCTGGTATGATTCTGGCTTTTAGTGCATCCGCTGCCCAGATCACCGACGGCAAGCA
GTACATCACCCTTGATAAGCCGATTGCTGGCGAGCCGCAGGTGCTCGAGTTCTTTTCGTTCTACTGCCCGCACTGCTACC
AGTTTGAAGAAGTGCTGCATGTCTCTGACAATGTGCGTCAGAAACTGCCGGAAGGCACTAAAATGACCAAATACCACGTT
GAGTTCCTGGGTCCGCTGGGCAAGGATCTGACGCAGGCATGGGCGGTGGCCATCGCGCTGGGCGTTGAAGACAAAATTAC
TGCGCCGATGTTCGAAGCCGTGCAGAAAAACCAGACTGTGCAGAGTGTGGCTGACATTCGTAAAGTGTTTGTCGACGCTG
GCGTGAAAGGGGAAGACTACGATGCCGCATGGAATAGCTTCGTGGTGAAATCTCTGGTCGCTCAGCAAGAGAAAGCGGCA
GCCGATCTGCAGCTGCAGGGCGTACCGGCTATGTATGTTAATGGTAAATACCAGCTGAATCCGCAGGGGATGGACACCAG
CAATATGGATGTCTTCGTCGCTCAGTATGCGGATACCGTGAAGCAACTGGTAGAGAAGAAATAA

Upstream 100 bases:

>100_bases
GCAGACGTCGACTTTTCTTGAGCAGGTGAAGGTTCTACAGGAACCCCCGCTTCAGTTAACGCCAATGTATTAATGTGATA
TATCCAGGAGAGAGTAAATC

Downstream 100 bases:

>100_bases
AAAAACGCCGGTCACTGACCGGCGTTACTTTATCTGTTTTGCAATAGCTACCGTTCTTTGTCCCGCATGATGTCAGTCAA
CTGACGGTCTTTCTCTTCCC

Product: periplasmic protein disulfide isomerase I

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MKKVWLALAGMILAFSASAAQITDGKQYITLDKPIAGEPQVLEFFSFYCPHCYQFEEVLHVSDNVRQKLPEGTKMTKYHV
EFLGPLGKDLTQAWAVAIALGVEDKITAPMFEAVQKNQTVQSVADIRKVFVDAGVKGEDYDAAWNSFVVKSLVAQQEKAA
ADLQLQGVPAMYVNGKYQLNPQGMDTSNMDVFVAQYADTVKQLVEKK

Sequences:

>Translated_207_residues
MKKVWLALAGMILAFSASAAQITDGKQYITLDKPIAGEPQVLEFFSFYCPHCYQFEEVLHVSDNVRQKLPEGTKMTKYHV
EFLGPLGKDLTQAWAVAIALGVEDKITAPMFEAVQKNQTVQSVADIRKVFVDAGVKGEDYDAAWNSFVVKSLVAQQEKAA
ADLQLQGVPAMYVNGKYQLNPQGMDTSNMDVFVAQYADTVKQLVEKK
>Mature_207_residues
MKKVWLALAGMILAFSASAAQITDGKQYITLDKPIAGEPQVLEFFSFYCPHCYQFEEVLHVSDNVRQKLPEGTKMTKYHV
EFLGPLGKDLTQAWAVAIALGVEDKITAPMFEAVQKNQTVQSVADIRKVFVDAGVKGEDYDAAWNSFVVKSLVAQQEKAA
ADLQLQGVPAMYVNGKYQLNPQGMDTSNMDVFVAQYADTVKQLVEKK

Specific function: Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. It is required for pilus biogenesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=208, Percent_Identity=81.25, Blast_Score=363, Evalue=1e-102,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 22946; Mature: 22946

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVWLALAGMILAFSASAAQITDGKQYITLDKPIAGEPQVLEFFSFYCPHCYQFEEVLH
CCHHHHHHHHHHHHHCCCCCEECCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHH
VSDNVRQKLPEGTKMTKYHVEFLGPLGKDLTQAWAVAIALGVEDKITAPMFEAVQKNQTV
HHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHH
QSVADIRKVFVDAGVKGEDYDAAWNSFVVKSLVAQQEKAAADLQLQGVPAMYVNGKYQLN
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEECCEEEEC
PQGMDTSNMDVFVAQYADTVKQLVEKK
CCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKVWLALAGMILAFSASAAQITDGKQYITLDKPIAGEPQVLEFFSFYCPHCYQFEEVLH
CCHHHHHHHHHHHHHCCCCCEECCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHH
VSDNVRQKLPEGTKMTKYHVEFLGPLGKDLTQAWAVAIALGVEDKITAPMFEAVQKNQTV
HHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHH
QSVADIRKVFVDAGVKGEDYDAAWNSFVVKSLVAQQEKAAADLQLQGVPAMYVNGKYQLN
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEECCEEEEC
PQGMDTSNMDVFVAQYADTVKQLVEKK
CCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA