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The map label for this gene is yieO [H]

Identifier: 238892269

GI number: 238892269

Start: 13492

End: 14889

Strand: Reverse

Name: yieO [H]

Synonym: KP1_0013

Alternate gene names: 238892269

Gene position: 14889-13492 (Counterclockwise)

Preceding gene: 238892270

Following gene: 238892261

Centisome position: 0.28

GC content: 56.44

Gene sequence:

>1398_bases
ATGAGCGGAAAAAAAGGGCGCAGCATGGCTGGCCTGCCGTGGATCGCGGCGATGGCCTTCTTTATGCAGGCGCTGGATGC
CACCATTCTTAACACCGCCCTCCCGGCTATCGCCCACAGTCTTAATCGTTCTCCCTTAGCGATGCAGTCGGCCATTATCA
GCTATACGCTGACGGTGGCGATGCTGATCCCGGTCAGCGGCTGGCTGGCCGATCGCTTCGGCACGCGCCGGGTCTTTATC
ATCGCCGTTAGCCTGTTCACCCTCGGTTCCCTCGCCTGCGCCCTCTCCAGTTCCTTAACGGAACTGGTCATTTTTCGCGT
AATTCAGGGGATCGGTGGGGCGATGATGATGCCGGTGGCGAGGCTGGCCCTGCTGCGTGCCTACCCCCGCAGCGAACTGT
TGCCGGTCCTGAACTTTGTCACCATGCCGGGATTGGTGGGGCCGATTCTGGGCCCGGTACTCGGCGGGGTATTTGTGACC
TGGGCCAGCTGGCATTGGATTTTCCTGATAAACATTCCGATCGGCGTAATTGGCATTCTGTACGCCCGTAAATACATGCC
AAATTTCACAACGCCGCGCCGACGCTTCGATATAGGCGGTTTTCTGTTATTTGGCTTAAGTCTGGTGTTATTTTCCAGCG
GTATTGAACTGTTTGGGGAAAAAATCGTGGCGACATGGCAGGCGCTGGCCGTGATTGCCGTCAGTCTGTTACTGCTCGTG
GCCTATGTGCGTCACGCGCGTCGCCATCCGTCGCCGCTTATCTCGCTCTCTTTATTTAAGACCCATACGTTCTCGGTCGG
CATTGCCGGCAACCTCGCCACGCGGTTAGGTACCGGCTGCGTACCGTTTCTGATGCCGCTGATGCTGCAGGTTGGTTTCG
GCTATCCGGCCATTATCGCCGGCTGTATGATCGCCCCCACCGCTATCGGCTCGATTATTGCGAAATCCACCGTTACCCAG
GTGCTGCGCTGGTTTGGCTATCGTAAGACCCTGGTGGGGATCACCGTGTTTATTGGCCTTATGATCGCCCAGTTCTCGCT
GCAGTCGCCAGAGATGCCGCTGTGGATGCTTCTTCTGCCGCTGTTCGTGCTGGGGATGGCGATGTCCACGCAGTTTACCG
CCATGAATACCATTACCCTGGCCGATCTGACCGATGATAATGCCAGCAGCGGCAACAGCCTGCTGGCCGTCACCCAGCAG
CTTTCAATTAGTCTCGGGGTGGCCATCAGCGCTGCGGTGCTACGCTTCTATGAAGGTTTTGATAATGCAAGCACGGTACA
GCAGTTTCACTACACCTTTATAACCATGGGCGTGATAACCATTATATCGGCGCTGATGTTTATGCTGTTAAGAGCGAAGG
ATGGCCGCAACCTGATTAGCGAGCGGCATAAACGCTAA

Upstream 100 bases:

>100_bases
TGTCGATGCCATTCTTAACAGTGATGCGCCGGGGGCGTTGCTCGCCTGCCAGGCATTGTTGAACGCCCCACACCACGTAA
ATCAATAACAAGGATTTGGC

Downstream 100 bases:

>100_bases
GCGGAACCGCGAACCACCAGCTCCGGCGTTAACTGAACGCGCTGCTGTTTTTGCTGCGGATCGGCCATGCGATGGATCAG
TACGTCGATGGCCAGTTCCC

Product: putative MFS-family transport protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSGKKGRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRVFI
IAVSLFTLGSLACALSSSLTELVIFRVIQGIGGAMMMPVARLALLRAYPRSELLPVLNFVTMPGLVGPILGPVLGGVFVT
WASWHWIFLINIPIGVIGILYARKYMPNFTTPRRRFDIGGFLLFGLSLVLFSSGIELFGEKIVATWQALAVIAVSLLLLV
AYVRHARRHPSPLISLSLFKTHTFSVGIAGNLATRLGTGCVPFLMPLMLQVGFGYPAIIAGCMIAPTAIGSIIAKSTVTQ
VLRWFGYRKTLVGITVFIGLMIAQFSLQSPEMPLWMLLLPLFVLGMAMSTQFTAMNTITLADLTDDNASSGNSLLAVTQQ
LSISLGVAISAAVLRFYEGFDNASTVQQFHYTFITMGVITIISALMFMLLRAKDGRNLISERHKR

Sequences:

>Translated_465_residues
MSGKKGRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRVFI
IAVSLFTLGSLACALSSSLTELVIFRVIQGIGGAMMMPVARLALLRAYPRSELLPVLNFVTMPGLVGPILGPVLGGVFVT
WASWHWIFLINIPIGVIGILYARKYMPNFTTPRRRFDIGGFLLFGLSLVLFSSGIELFGEKIVATWQALAVIAVSLLLLV
AYVRHARRHPSPLISLSLFKTHTFSVGIAGNLATRLGTGCVPFLMPLMLQVGFGYPAIIAGCMIAPTAIGSIIAKSTVTQ
VLRWFGYRKTLVGITVFIGLMIAQFSLQSPEMPLWMLLLPLFVLGMAMSTQFTAMNTITLADLTDDNASSGNSLLAVTQQ
LSISLGVAISAAVLRFYEGFDNASTVQQFHYTFITMGVITIISALMFMLLRAKDGRNLISERHKR
>Mature_464_residues
SGKKGRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRVFII
AVSLFTLGSLACALSSSLTELVIFRVIQGIGGAMMMPVARLALLRAYPRSELLPVLNFVTMPGLVGPILGPVLGGVFVTW
ASWHWIFLINIPIGVIGILYARKYMPNFTTPRRRFDIGGFLLFGLSLVLFSSGIELFGEKIVATWQALAVIAVSLLLLVA
YVRHARRHPSPLISLSLFKTHTFSVGIAGNLATRLGTGCVPFLMPLMLQVGFGYPAIIAGCMIAPTAIGSIIAKSTVTQV
LRWFGYRKTLVGITVFIGLMIAQFSLQSPEMPLWMLLLPLFVLGMAMSTQFTAMNTITLADLTDDNASSGNSLLAVTQQL
SISLGVAISAAVLRFYEGFDNASTVQQFHYTFITMGVITIISALMFMLLRAKDGRNLISERHKR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=464, Percent_Identity=84.4827586206897, Blast_Score=791, Evalue=0.0,
Organism=Escherichia coli, GI1788392, Length=458, Percent_Identity=45.4148471615721, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1789042, Length=429, Percent_Identity=30.7692307692308, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1788710, Length=432, Percent_Identity=30.787037037037, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI87081983, Length=299, Percent_Identity=29.7658862876254, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87082312, Length=185, Percent_Identity=31.3513513513514, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1788509, Length=136, Percent_Identity=28.6764705882353, Blast_Score=62, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6322958, Length=471, Percent_Identity=26.963906581741, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=167, Percent_Identity=28.7425149700599, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=147, Percent_Identity=31.2925170068027, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50473; Mature: 50342

Theoretical pI: Translated: 10.97; Mature: 10.97

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKKGRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MLIPVSGWLADRFGTRRVFIIAVSLFTLGSLACALSSSLTELVIFRVIQGIGGAMMMPVA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RLALLRAYPRSELLPVLNFVTMPGLVGPILGPVLGGVFVTWASWHWIFLINIPIGVIGIL
HHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHH
YARKYMPNFTTPRRRFDIGGFLLFGLSLVLFSSGIELFGEKIVATWQALAVIAVSLLLLV
HHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AYVRHARRHPSPLISLSLFKTHTFSVGIAGNLATRLGTGCVPFLMPLMLQVGFGYPAIIA
HHHHHHHCCCCCEEEHHHHHHHEEEEEECHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHH
GCMIAPTAIGSIIAKSTVTQVLRWFGYRKTLVGITVFIGLMIAQFSLQSPEMPLWMLLLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LFVLGMAMSTQFTAMNTITLADLTDDNASSGNSLLAVTQQLSISLGVAISAAVLRFYEGF
HHHHHHHHHCHHHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DNASTVQQFHYTFITMGVITIISALMFMLLRAKDGRNLISERHKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure 
SGKKGRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MLIPVSGWLADRFGTRRVFIIAVSLFTLGSLACALSSSLTELVIFRVIQGIGGAMMMPVA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RLALLRAYPRSELLPVLNFVTMPGLVGPILGPVLGGVFVTWASWHWIFLINIPIGVIGIL
HHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHH
YARKYMPNFTTPRRRFDIGGFLLFGLSLVLFSSGIELFGEKIVATWQALAVIAVSLLLLV
HHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AYVRHARRHPSPLISLSLFKTHTFSVGIAGNLATRLGTGCVPFLMPLMLQVGFGYPAIIA
HHHHHHHCCCCCEEEHHHHHHHEEEEEECHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHH
GCMIAPTAIGSIIAKSTVTQVLRWFGYRKTLVGITVFIGLMIAQFSLQSPEMPLWMLLLP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LFVLGMAMSTQFTAMNTITLADLTDDNASSGNSLLAVTQQLSISLGVAISAAVLRFYEGF
HHHHHHHHHCHHHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DNASTVQQFHYTFITMGVITIISALMFMLLRAKDGRNLISERHKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]