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The map label for this gene is rbsR [H]

Identifier: 238892268

GI number: 238892268

Start: 12512

End: 13495

Strand: Direct

Name: rbsR [H]

Synonym: KP1_0012

Alternate gene names: 238892268

Gene position: 12512-13495 (Clockwise)

Preceding gene: 238892267

Following gene: 238892273

Centisome position: 0.24

GC content: 57.83

Gene sequence:

>984_bases
ATGAAAGATGTGGCCCGCATCGCGGGCGTTTCCACATCGACGGTGTCACACGTCATCAATAAGGACCGCTTTGTCAGCGA
GGCGATTACCGCCAAAGTTGACGCTGCGATCAAAAGCCTCAACTACGCCCCCTCTGCGCTGGCGCGTAGCCTCAAGCTCA
ATCAAACCCGTACCATCGGCATGCTGATCACCGCCAGTACCAATCCGTTTTATTCGGAGCTGGTGCGGGGCGTGGAGCGT
AGCTGCTTTGAGCGGGGTTATAGCCTCGTGCTCTGCAATACCGAAGGTGATGAGCAGCGAATGAATCGTAATCTGGAGAC
GCTAATGCAAAAACGCGTCGATGGATTACTCCTGCTGTGCACGGAGACTCATCAGCCCTCCCCGGAAATCATGCATCGCT
ATCCGTCGGTACCGACGGTTATGATGGACTGGGCCCCCTTTGATGGCGATAGCGATCTGATTCAGGATAACTCGCTGCTC
GGCGGCGATATGGCGACCCAGTATCTGATTGACCAGGGGCATTCACGCATTGCCTGTATTGCCGGCCCGCTGGATAAAAC
CCCGGCGCGCCTGCGTCTTGAAGGCTATCATGCCGCGATGGCGCGCTGCGGCCTGCCGGTTGCCGAAGGATATGTGGTGA
CCAGTGATTTTGAGTTTGGCGGCGGGTTTAGCGCGATGCAGCAGCTGCTGGCGTTGCCGCAGCGTCCGCAGGCGGTGTTT
GTCGGTAACGATGCGATGGCGGTTGGCGCTTATCAGGCGTTATACCAGGCCGGGCTGCAGATTCCGCAGGATATGGCGCT
GGTGGGCTACGATGATATTGAGCTGGCACGTTATATGACGCCGCCATTGACCACCATTCACCAGCCAAAGGATGAGCTGG
GGGAACTGGCCATCGACGTACTGATCCATCGCATGGCCGATCCGCAGCAAAAACAGCAGCGCGTTCAGTTAACGCCGGAG
CTGGTGGTTCGCGGTTCCGCTTAG

Upstream 100 bases:

>100_bases
AGCGGCGATCGCCGTAACCCGCAAAGGCGCTCAGCCGTCCGTGCCGTGGCGCACAGAGATCGATGAATTTTTAGCGCAAC
AGGGGTAATGCTTGGCTACG

Downstream 100 bases:

>100_bases
CGTTTATGCCGCTCGCTAATCAGGTTGCGGCCATCCTTCGCTCTTAACAGCATAAACATCAGCGCCGATATAATGGTTAT
CACGCCCATGGTTATAAAGG

Product: transcriptional repressor RbsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKDVARIAGVSTSTVSHVINKDRFVSEAITAKVDAAIKSLNYAPSALARSLKLNQTRTIGMLITASTNPFYSELVRGVER
SCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSPEIMHRYPSVPTVMMDWAPFDGDSDLIQDNSLL
GGDMATQYLIDQGHSRIACIAGPLDKTPARLRLEGYHAAMARCGLPVAEGYVVTSDFEFGGGFSAMQQLLALPQRPQAVF
VGNDAMAVGAYQALYQAGLQIPQDMALVGYDDIELARYMTPPLTTIHQPKDELGELAIDVLIHRMADPQQKQQRVQLTPE
LVVRGSA

Sequences:

>Translated_327_residues
MKDVARIAGVSTSTVSHVINKDRFVSEAITAKVDAAIKSLNYAPSALARSLKLNQTRTIGMLITASTNPFYSELVRGVER
SCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSPEIMHRYPSVPTVMMDWAPFDGDSDLIQDNSLL
GGDMATQYLIDQGHSRIACIAGPLDKTPARLRLEGYHAAMARCGLPVAEGYVVTSDFEFGGGFSAMQQLLALPQRPQAVF
VGNDAMAVGAYQALYQAGLQIPQDMALVGYDDIELARYMTPPLTTIHQPKDELGELAIDVLIHRMADPQQKQQRVQLTPE
LVVRGSA
>Mature_327_residues
MKDVARIAGVSTSTVSHVINKDRFVSEAITAKVDAAIKSLNYAPSALARSLKLNQTRTIGMLITASTNPFYSELVRGVER
SCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSPEIMHRYPSVPTVMMDWAPFDGDSDLIQDNSLL
GGDMATQYLIDQGHSRIACIAGPLDKTPARLRLEGYHAAMARCGLPVAEGYVVTSDFEFGGGFSAMQQLLALPQRPQAVF
VGNDAMAVGAYQALYQAGLQIPQDMALVGYDDIELARYMTPPLTTIHQPKDELGELAIDVLIHRMADPQQKQQRVQLTPE
LVVRGSA

Specific function: Transcriptional repressor for the ribose rbsDACBK operon. RbsR binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site. The affinity for the rbs operator is reduced by addition of ribose, consistent with ribose being

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=327, Percent_Identity=86.2385321100918, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1787948, Length=327, Percent_Identity=46.4831804281346, Blast_Score=319, Evalue=1e-88,
Organism=Escherichia coli, GI1790369, Length=328, Percent_Identity=35.9756097560976, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI1789202, Length=290, Percent_Identity=33.1034482758621, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1786540, Length=310, Percent_Identity=32.9032258064516, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1787580, Length=311, Percent_Identity=29.5819935691318, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788474, Length=328, Percent_Identity=28.6585365853659, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1789068, Length=304, Percent_Identity=27.3026315789474, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI48994940, Length=308, Percent_Identity=29.8701298701299, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1787906, Length=331, Percent_Identity=25.0755287009063, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789456, Length=338, Percent_Identity=26.6272189349112, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786268, Length=320, Percent_Identity=24.375, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790715, Length=319, Percent_Identity=24.4514106583072, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1790192, Length=267, Percent_Identity=28.0898876404494, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1790689, Length=323, Percent_Identity=24.1486068111455, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35929; Mature: 35929

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDVARIAGVSTSTVSHVINKDRFVSEAITAKVDAAIKSLNYAPSALARSLKLNQTRTIG
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEE
MLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLC
EEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHCCCEEEEE
TETHQPSPEIMHRYPSVPTVMMDWAPFDGDSDLIQDNSLLGGDMATQYLIDQGHSRIACI
ECCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEE
AGPLDKTPARLRLEGYHAAMARCGLPVAEGYVVTSDFEFGGGFSAMQQLLALPQRPQAVF
ECCCCCCCCEEEECHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCEEE
VGNDAMAVGAYQALYQAGLQIPQDMALVGYDDIELARYMTPPLTTIHQPKDELGELAIDV
ECCCCHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LIHRMADPQQKQQRVQLTPELVVRGSA
HHHHHCCHHHHHHHHCCCHHHEEECCC
>Mature Secondary Structure
MKDVARIAGVSTSTVSHVINKDRFVSEAITAKVDAAIKSLNYAPSALARSLKLNQTRTIG
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEE
MLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLC
EEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHCCCEEEEE
TETHQPSPEIMHRYPSVPTVMMDWAPFDGDSDLIQDNSLLGGDMATQYLIDQGHSRIACI
ECCCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEE
AGPLDKTPARLRLEGYHAAMARCGLPVAEGYVVTSDFEFGGGFSAMQQLLALPQRPQAVF
ECCCCCCCCEEEECHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCEEE
VGNDAMAVGAYQALYQAGLQIPQDMALVGYDDIELARYMTPPLTTIHQPKDELGELAIDV
ECCCCHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LIHRMADPQQKQQRVQLTPELVVRGSA
HHHHHCCHHHHHHHHCCCHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]