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The map label for this gene is mnmE [H]

Identifier: 238028966

GI number: 238028966

Start: 3900374

End: 3901792

Strand: Reverse

Name: mnmE [H]

Synonym: bglu_1g34350

Alternate gene names: 238028966

Gene position: 3901792-3900374 (Counterclockwise)

Preceding gene: 238028969

Following gene: 238028965

Centisome position: 99.88

GC content: 69.13

Gene sequence:

>1419_bases
ATGCTGACTACCGATTCCGATCCAATCGTTGCCATTGCCACCGCGCCCGGGCGGGGCGGGATCGGGGTGGTGCGAATCTC
GTTCGGCCGCGCCGGCGCGAGCGCGGCCGAGGCCTTGATGCAGGCCGTGTGCGGGCAATTGCTGGCCGCGCGCCACGCCA
GCTACGTGCCGTTTCTCGACGGCGCGGGCGAGGCGCTCGATCGCGGCATCGCGCTCAATTTCCCGGCACCGAACTCGTAT
ACCGGCGAGCATGTACTCGAACTGCAGGGCCATGGCGGCCCCATCGTGCTGCAGCTCTTGCTGCAGCGCTGCCTCGATGC
CGGCCGCGGCCACGGCGTGCGGCTTGCCGAGCCCGGCGAATTTACGCGCCGCGCGTTCCTGAACGACAAGCTCGATCTCG
CGCAGGCCGAGGCGGTCGCGGACCTGATCGAGGCCAGCACGGAGGCGGCGGCGCGCTCGGCCGGACGCTCGCTCGACGGC
GCGTTCTCGCGCGACGTTCATGCGCTCGTCGACGACGTGATCGGGCTGCGCATGCTGGTCGAGGCGACGCTCGATTTTCC
TGAGGAGGAGATCGATTTCCTCGAGGCTGCCGACGCGCGCGGCAAGCTCGCGCGGATTCGCGAACGGCTCGCGCGCGTGC
TCGGCGACGCGCGCCAGGGCGCGCTGCTGCGCGAGGGGCTGTCGGTGGTGCTGGCCGGGCAGCCGAACGTCGGGAAATCG
TCGCTGCTGAACGCGCTGGCCGGCGCCGAGCTCGCGATCGTCACACCGATCGCGGGGACCACACGCGACAAGGTCGCGCA
GACGATCCAGATCGAAGGGATTCCGCTGCATATCGTCGATACGGCCGGGTTGCGCGAAACCGAAGACGAGGTCGAGAAGA
TCGGCATCGCACGGACCTGGGGCGAGATCGAGCGCGCCGATGTCGTGCTGCATCTGCTCGATGCGCGCGAGGGGCTCGGT
GCGGACGATCGCGCGATTGCCGGGCGTTTCCCGGCCGGCGTGCCGGTCGTGCGCGTCTTCAACAAGACCGATCTGACCGA
CGCGCCGCCGGCGGTCGCGCATCTCGGCGGCCATGCGCAGGCGGACGCGGCCGGCGGGCTCGATCTGAGCGAGGTCAGGC
TGTCTGCGAAACGCGGCGACGGCATCGACCTGCTGCGCGCCGAACTGCTGCGAATCGCCGGCTGGCAGGCGGGCGCCGAG
AGCGTCTACCTGGCGCGCGAACGGCATCTGATCGCGCTGCGCGCGGCGCAGGCGCACCTCGCGCAGGCGGCGGGCCATGC
CGATCAGAACGCCGAGGCGCTCGACCTGTTCGCGGAAGAGTTGCGACTCGCGCAGGAGCAACTCAATTCGATTACCGGCG
AATTCACTTCCGATGATTTGCTGGGGGTGATTTTTAGTCGGTTTTGCATCGGCAAATGA

Upstream 100 bases:

>100_bases
GGCTGCCGTCGGCCGCGTGAAGGCCGGCACGCTACAATGCGGCGTTGCGCCGGCATGGTTGTCACGCCGTCCCGTTTCGT
ATTCCTCCCGCGATTTTCCG

Downstream 100 bases:

>100_bases
GTGGCTTCCAACCAATGCCAAAGATCACCAACGAAACCCCCACGTAGAAAGGGCTTCAGCCCAAATTCTGCACAAAATTT
CTCCATCATTCCCCATCGAC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MLTTDSDPIVAIATAPGRGGIGVVRISFGRAGASAAEALMQAVCGQLLAARHASYVPFLDGAGEALDRGIALNFPAPNSY
TGEHVLELQGHGGPIVLQLLLQRCLDAGRGHGVRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRDVHALVDDVIGLRMLVEATLDFPEEEIDFLEAADARGKLARIRERLARVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQIEGIPLHIVDTAGLRETEDEVEKIGIARTWGEIERADVVLHLLDAREGLG
ADDRAIAGRFPAGVPVVRVFNKTDLTDAPPAVAHLGGHAQADAAGGLDLSEVRLSAKRGDGIDLLRAELLRIAGWQAGAE
SVYLARERHLIALRAAQAHLAQAAGHADQNAEALDLFAEELRLAQEQLNSITGEFTSDDLLGVIFSRFCIGK

Sequences:

>Translated_472_residues
MLTTDSDPIVAIATAPGRGGIGVVRISFGRAGASAAEALMQAVCGQLLAARHASYVPFLDGAGEALDRGIALNFPAPNSY
TGEHVLELQGHGGPIVLQLLLQRCLDAGRGHGVRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRDVHALVDDVIGLRMLVEATLDFPEEEIDFLEAADARGKLARIRERLARVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQIEGIPLHIVDTAGLRETEDEVEKIGIARTWGEIERADVVLHLLDAREGLG
ADDRAIAGRFPAGVPVVRVFNKTDLTDAPPAVAHLGGHAQADAAGGLDLSEVRLSAKRGDGIDLLRAELLRIAGWQAGAE
SVYLARERHLIALRAAQAHLAQAAGHADQNAEALDLFAEELRLAQEQLNSITGEFTSDDLLGVIFSRFCIGK
>Mature_472_residues
MLTTDSDPIVAIATAPGRGGIGVVRISFGRAGASAAEALMQAVCGQLLAARHASYVPFLDGAGEALDRGIALNFPAPNSY
TGEHVLELQGHGGPIVLQLLLQRCLDAGRGHGVRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRDVHALVDDVIGLRMLVEATLDFPEEEIDFLEAADARGKLARIRERLARVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQIEGIPLHIVDTAGLRETEDEVEKIGIARTWGEIERADVVLHLLDAREGLG
ADDRAIAGRFPAGVPVVRVFNKTDLTDAPPAVAHLGGHAQADAAGGLDLSEVRLSAKRGDGIDLLRAELLRIAGWQAGAE
SVYLARERHLIALRAAQAHLAQAAGHADQNAEALDLFAEELRLAQEQLNSITGEFTSDDLLGVIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=486, Percent_Identity=36.6255144032922, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI193082993, Length=476, Percent_Identity=36.1344537815126, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI193082988, Length=516, Percent_Identity=34.6899224806202, Blast_Score=205, Evalue=6e-53,
Organism=Escherichia coli, GI2367268, Length=473, Percent_Identity=54.5454545454545, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI87082120, Length=122, Percent_Identity=37.7049180327869, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=473, Percent_Identity=28.5412262156448, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323665, Length=490, Percent_Identity=28.5714285714286, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI45550813, Length=508, Percent_Identity=32.8740157480315, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49744; Mature: 49744

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTTDSDPIVAIATAPGRGGIGVVRISFGRAGASAAEALMQAVCGQLLAARHASYVPFLD
CCCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GAGEALDRGIALNFPAPNSYTGEHVLELQGHGGPIVLQLLLQRCLDAGRGHGVRLAEPGE
CCCHHHHCCEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEECCCCH
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDGAFSRDVHALVDDVIGLRMLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
EATLDFPEEEIDFLEAADARGKLARIRERLARVLGDARQGALLREGLSVVLAGQPNVGKS
HHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHCCHHHHCCCEEEEECCCCCCHH
SLLNALAGAELAIVTPIAGTTRDKVAQTIQIEGIPLHIVDTAGLRETEDEVEKIGIARTW
HHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCEEEEEEECCCCCCCHHHHHHHCHHHHH
GEIERADVVLHLLDAREGLGADDRAIAGRFPAGVPVVRVFNKTDLTDAPPAVAHLGGHAQ
HHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCEEEEECCCCCCCCCCHHHHCCCCCC
ADAAGGLDLSEVRLSAKRGDGIDLLRAELLRIAGWQAGAESVYLARERHLIALRAAQAHL
CCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEHHHCCEEEHHHHHHHH
AQAAGHADQNAEALDLFAEELRLAQEQLNSITGEFTSDDLLGVIFSRFCIGK
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTTDSDPIVAIATAPGRGGIGVVRISFGRAGASAAEALMQAVCGQLLAARHASYVPFLD
CCCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GAGEALDRGIALNFPAPNSYTGEHVLELQGHGGPIVLQLLLQRCLDAGRGHGVRLAEPGE
CCCHHHHCCEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEECCCCH
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDGAFSRDVHALVDDVIGLRMLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
EATLDFPEEEIDFLEAADARGKLARIRERLARVLGDARQGALLREGLSVVLAGQPNVGKS
HHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHCCHHHHCCCEEEEECCCCCCHH
SLLNALAGAELAIVTPIAGTTRDKVAQTIQIEGIPLHIVDTAGLRETEDEVEKIGIARTW
HHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCEEEEEEECCCCCCCHHHHHHHCHHHHH
GEIERADVVLHLLDAREGLGADDRAIAGRFPAGVPVVRVFNKTDLTDAPPAVAHLGGHAQ
HHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCEEEEECCCCCCCCCCHHHHCCCCCC
ADAAGGLDLSEVRLSAKRGDGIDLLRAELLRIAGWQAGAESVYLARERHLIALRAAQAHL
CCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEHHHCCEEEHHHHHHHH
AQAAGHADQNAEALDLFAEELRLAQEQLNSITGEFTSDDLLGVIFSRFCIGK
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA