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The map label for this gene is yafC [C]

Identifier: 238028949

GI number: 238028949

Start: 3881075

End: 3882133

Strand: Reverse

Name: yafC [C]

Synonym: bglu_1g34140

Alternate gene names: 238028949

Gene position: 3882133-3881075 (Counterclockwise)

Preceding gene: 238028951

Following gene: 238028945

Centisome position: 99.38

GC content: 67.71

Gene sequence:

>1059_bases
ATGGATCATCTGCAGTCGATGCGAGTGTTCGTCAAGGTGGCGGATCTGGGCAGCTTCGCGCGAGCGGCCAGTGCGATGGA
TATCTCGAACGCCGTGGCCACGCGCCACGTTGCCGATCTCGAAGGCCGGCTCGGCACCCGGTTGCTGAACCGGACGACGC
GCAGCCTTTCCCTGACCGAATCCGGCCAGGTCTATCTCGAGCGGGCTCGCCAGATTCTCGACGAACTCGAGGACGTCGAG
CAGATGGTGGTCGCGCGCAACCACGAACCGGTCGGCACCTTGCGGATCGTCGCGCCGGTGGTGTTCGGGCTGCACAATCT
CGCGCCCGTGCTGCAAACCTATACCCAGCGCTATCCGAAGGTCGTCCCCGACCTCACGCTGGTCGACCGGCAGGTCGATC
TGGTCGAGGAGGGCTTCGACGTCGGCGTCGTGATCGCGCGGCAGATGCGCAGCGCTAGCATCGTCACGCGGCGCCTGACC
ACAGGTTGCATGACGGTCTGCGCGACGCCGGCTTATCTGGAAAAGCATGGCGTGCCAACCCATCCCGAGCATCTGATGGA
GCATCCGTCCCTGAGCCTGCCGTCCGAATACTGGGGCGACGAGCGTGTTTTCACGGGGCCGGACGGCGAGGTGCGGGTGC
GGCCGACCAACGTGGTGGTTGCCAACAACACCGAAATGCTGCGCCAGTTCGCACTGCTCGGCATGGGCATCGCGATGCTG
CCGAGCTATCTGATCGGCGGCGACACGGCACGCGGCAAGCTGGTGCGCCTGCTGCCCGACTACCGTCTGCCGCAGGTCGA
GATCAACGTCGCCTACCCGAGCCGCCGCCACCTGCCGGCCAAGGTGCGCACGTTCATCGACCATCTGGTCGAGCACTTCA
GCCACTCGCCGGAAACGGCCTTAGGCGAGCAATGGGTCGCGCACGGCGGCCTGCAGCCGCCGTCCGAGCCCGCTGCACCG
GGCGCTGCGGTGAGCGGCACGCGTCCGGCCGCCGGCCCGTCGATGCCGGCGCCGCTGCCGAAGGCGCGCCAGCGCGCGAC
CGCGCCGACTCCGCTGTAA

Upstream 100 bases:

>100_bases
CAACCTTCGGCGGCAACTGTGCATCAAATGCGCTGTCGTAGGAAAATTACGCATTTGCTAGACAATTTTGAAGCTGTAAT
GCACAGCGGCGGGAGTGGTT

Downstream 100 bases:

>100_bases
GCCGTGGCGGCCGAATGGCCGCCTGCTGCTCAAGCGAAAACGCACTGCCCGTGAGGGCAGTGCGTTTTTTCATGGCGTCG
GTCGGCGAGGCGGCGGAAAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MDHLQSMRVFVKVADLGSFARAASAMDISNAVATRHVADLEGRLGTRLLNRTTRSLSLTESGQVYLERARQILDELEDVE
QMVVARNHEPVGTLRIVAPVVFGLHNLAPVLQTYTQRYPKVVPDLTLVDRQVDLVEEGFDVGVVIARQMRSASIVTRRLT
TGCMTVCATPAYLEKHGVPTHPEHLMEHPSLSLPSEYWGDERVFTGPDGEVRVRPTNVVVANNTEMLRQFALLGMGIAML
PSYLIGGDTARGKLVRLLPDYRLPQVEINVAYPSRRHLPAKVRTFIDHLVEHFSHSPETALGEQWVAHGGLQPPSEPAAP
GAAVSGTRPAAGPSMPAPLPKARQRATAPTPL

Sequences:

>Translated_352_residues
MDHLQSMRVFVKVADLGSFARAASAMDISNAVATRHVADLEGRLGTRLLNRTTRSLSLTESGQVYLERARQILDELEDVE
QMVVARNHEPVGTLRIVAPVVFGLHNLAPVLQTYTQRYPKVVPDLTLVDRQVDLVEEGFDVGVVIARQMRSASIVTRRLT
TGCMTVCATPAYLEKHGVPTHPEHLMEHPSLSLPSEYWGDERVFTGPDGEVRVRPTNVVVANNTEMLRQFALLGMGIAML
PSYLIGGDTARGKLVRLLPDYRLPQVEINVAYPSRRHLPAKVRTFIDHLVEHFSHSPETALGEQWVAHGGLQPPSEPAAP
GAAVSGTRPAAGPSMPAPLPKARQRATAPTPL
>Mature_352_residues
MDHLQSMRVFVKVADLGSFARAASAMDISNAVATRHVADLEGRLGTRLLNRTTRSLSLTESGQVYLERARQILDELEDVE
QMVVARNHEPVGTLRIVAPVVFGLHNLAPVLQTYTQRYPKVVPDLTLVDRQVDLVEEGFDVGVVIARQMRSASIVTRRLT
TGCMTVCATPAYLEKHGVPTHPEHLMEHPSLSLPSEYWGDERVFTGPDGEVRVRPTNVVVANNTEMLRQFALLGMGIAML
PSYLIGGDTARGKLVRLLPDYRLPQVEINVAYPSRRHLPAKVRTFIDHLVEHFSHSPETALGEQWVAHGGLQPPSEPAAP
GAAVSGTRPAAGPSMPAPLPKARQRATAPTPL

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=300, Percent_Identity=34, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=33.3333333333333, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI87081978, Length=311, Percent_Identity=32.475884244373, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=29.8969072164948, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1787128, Length=302, Percent_Identity=28.8079470198675, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1789440, Length=287, Percent_Identity=29.2682926829268, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=28.6206896551724, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789173, Length=252, Percent_Identity=27.7777777777778, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790011, Length=307, Percent_Identity=24.1042345276873, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI157672245, Length=143, Percent_Identity=29.3706293706294, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787879, Length=151, Percent_Identity=30.4635761589404, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788481, Length=143, Percent_Identity=28.6713286713287, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 38546; Mature: 38546

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHLQSMRVFVKVADLGSFARAASAMDISNAVATRHVADLEGRLGTRLLNRTTRSLSLTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
SGQVYLERARQILDELEDVEQMVVARNHEPVGTLRIVAPVVFGLHNLAPVLQTYTQRYPK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VVPDLTLVDRQVDLVEEGFDVGVVIARQMRSASIVTRRLTTGCMTVCATPAYLEKHGVPT
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
HPEHLMEHPSLSLPSEYWGDERVFTGPDGEVRVRPTNVVVANNTEMLRQFALLGMGIAML
CHHHHHCCCCCCCCHHHCCCCCEEECCCCCEEEEECEEEEECCHHHHHHHHHHHHHHHHH
PSYLIGGDTARGKLVRLLPDYRLPQVEINVAYPSRRHLPAKVRTFIDHLVEHFSHSPETA
HHHHHCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHH
LGEQWVAHGGLQPPSEPAAPGAAVSGTRPAAGPSMPAPLPKARQRATAPTPL
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MDHLQSMRVFVKVADLGSFARAASAMDISNAVATRHVADLEGRLGTRLLNRTTRSLSLTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
SGQVYLERARQILDELEDVEQMVVARNHEPVGTLRIVAPVVFGLHNLAPVLQTYTQRYPK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VVPDLTLVDRQVDLVEEGFDVGVVIARQMRSASIVTRRLTTGCMTVCATPAYLEKHGVPT
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
HPEHLMEHPSLSLPSEYWGDERVFTGPDGEVRVRPTNVVVANNTEMLRQFALLGMGIAML
CHHHHHCCCCCCCCHHHCCCCCEEECCCCCEEEEECEEEEECCHHHHHHHHHHHHHHHHH
PSYLIGGDTARGKLVRLLPDYRLPQVEINVAYPSRRHLPAKVRTFIDHLVEHFSHSPETA
HHHHHCCCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHH
LGEQWVAHGGLQPPSEPAAPGAAVSGTRPAAGPSMPAPLPKARQRATAPTPL
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]