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The map label for this gene is def1 [H]

Identifier: 238028945

GI number: 238028945

Start: 3875590

End: 3876093

Strand: Reverse

Name: def1 [H]

Synonym: bglu_1g34100

Alternate gene names: 238028945

Gene position: 3876093-3875590 (Counterclockwise)

Preceding gene: 238028949

Following gene: 238028944

Centisome position: 99.22

GC content: 64.09

Gene sequence:

>504_bases
ATGGCGCTTCTGAATATCCTGCATTACCCCGACAAACGGCTGCACAAGGTGGCCAAACCCGTGGCGGTCGTCGACGACCG
GATCCGCAAGCTCGTCGCCGACATGGCCGAGACCATGTACGCGGCGCCCGGCATCGGCCTCGCGGCGACCCAGGTCGACG
TCCACGAGCGTGTGATCGTGATCGACACGTCCGAGGACAAGAACGAGCTGCGCGTGTTCATCAACCCAGAGATCGTCTGG
TCGAGCGACGGCAAGCAGATCTACGAGGAAGGCTGCCTCTCGGTGCCGGGCGTCTACGACGAGGTCGAGCGGCCCGATCA
CGTGCGCGTGCGCGCGCTCGACGTGCAGGGCCAGCAGTTCGAACTCGACTGCGAGGGCCTGCTGGCCGTGTGCGTGCAGC
ACGAGATGGATCACCTGCTCGGCCGCGTGTTCGTGCAGTACCTGTCGCCGCTCAAGCAGAACCGCATCAAGTCCAAGATG
AAGAAGCTCGAACGCGCGATGTGA

Upstream 100 bases:

>100_bases
GGCTTCATTTTGCGGATCCCGCTACGGCCCGGCCAGTCGGCCGGACGGCCGAGCCGGCCGGCGCGCAAGGCCGCACCAAC
CGCTTTCCGAAACGAACATC

Downstream 100 bases:

>100_bases
CGCCCGTGATTGATCGATCCTCCATGACTCATTCGCTGCGCGTCATCTTCGCCGGTACGCCGGAATTCGCCGCGGCGGCG
CTCGCCGCCATCCACGAGGC

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MALLNILHYPDKRLHKVAKPVAVVDDRIRKLVADMAETMYAAPGIGLAATQVDVHERVIVIDTSEDKNELRVFINPEIVW
SSDGKQIYEEGCLSVPGVYDEVERPDHVRVRALDVQGQQFELDCEGLLAVCVQHEMDHLLGRVFVQYLSPLKQNRIKSKM
KKLERAM

Sequences:

>Translated_167_residues
MALLNILHYPDKRLHKVAKPVAVVDDRIRKLVADMAETMYAAPGIGLAATQVDVHERVIVIDTSEDKNELRVFINPEIVW
SSDGKQIYEEGCLSVPGVYDEVERPDHVRVRALDVQGQQFELDCEGLLAVCVQHEMDHLLGRVFVQYLSPLKQNRIKSKM
KKLERAM
>Mature_166_residues
ALLNILHYPDKRLHKVAKPVAVVDDRIRKLVADMAETMYAAPGIGLAATQVDVHERVIVIDTSEDKNELRVFINPEIVWS
SDGKQIYEEGCLSVPGVYDEVERPDHVRVRALDVQGQQFELDCEGLLAVCVQHEMDHLLGRVFVQYLSPLKQNRIKSKMK
KLERAM

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=165, Percent_Identity=57.5757575757576, Blast_Score=196, Evalue=7e-52,
Organism=Drosophila melanogaster, GI24645728, Length=143, Percent_Identity=34.965034965035, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24645726, Length=142, Percent_Identity=30.9859154929577, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19004; Mature: 18873

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLNILHYPDKRLHKVAKPVAVVDDRIRKLVADMAETMYAAPGIGLAATQVDVHERVIV
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEE
IDTSEDKNELRVFINPEIVWSSDGKQIYEEGCLSVPGVYDEVERPDHVRVRALDVQGQQF
EECCCCCCCEEEEECCEEEECCCCHHHHHCCCCCCCCCHHHCCCCCCEEEEEEECCCCEE
ELDCEGLLAVCVQHEMDHLLGRVFVQYLSPLKQNRIKSKMKKLERAM
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALLNILHYPDKRLHKVAKPVAVVDDRIRKLVADMAETMYAAPGIGLAATQVDVHERVIV
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEE
IDTSEDKNELRVFINPEIVWSSDGKQIYEEGCLSVPGVYDEVERPDHVRVRALDVQGQQF
EECCCCCCCEEEEECCEEEECCCCHHHHHCCCCCCCCCHHHCCCCCCEEEEEEECCCCEE
ELDCEGLLAVCVQHEMDHLLGRVFVQYLSPLKQNRIKSKMKKLERAM
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]