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The map label for this gene is htrB [H]

Identifier: 238028904

GI number: 238028904

Start: 3830789

End: 3831673

Strand: Direct

Name: htrB [H]

Synonym: bglu_1g33690

Alternate gene names: 238028904

Gene position: 3830789-3831673 (Clockwise)

Preceding gene: 238028902

Following gene: 238028905

Centisome position: 98.06

GC content: 67.57

Gene sequence:

>885_bases
ATGCTAGGCCGTCTCGGCACCCGCTTCGCGCTGCTCTTGCTGAAGCTGTTCGCGCTGCTGCCGTATGGCTTGACGGCGCG
TTTCGGCGACGCGCTGGGCTGGCTGCTGTACCGTATCCCGAGCCGCCGCAAGCGCATCGTGCATACGAACCTGAAGCTCT
GCTTTCCCGAGTGGAGCGAGGCGCGGCGCGAGGAAGTCGCGGGCCGGCACTTTCGCCATGCGATTCGCAGCTACGTCGAG
CGCAGCGTCCAGTGGTTCGGCTCCGAGAAGAAGCTCGCCCGGCTCATCGAGGTCGACAGCGCGATCGACCTGACCGATCC
GGACCTGCCGCCCACGCTGTTTCTCGGCCTGCATTTCGTCGGCATCGAGGCCGGCTCGATCTGGCTGAACCGCTCGCTGC
AACGCACCTGCGGTTCGTTGTACCAACCGTTTTCAAACCCGCTGCTCGAGGCCGAGGCCAAGCGCGCGCGCGCGCGCTTC
GACGCCGAGATGGCGAGCCGCGCCGACAGCGCGCGCATCGTGCTGCGCTGGCTGCGCGAGCGCAAGCCCGTGATGCTCGG
CGCCGACATGGACTACGGCACGCGCAACTCCACCTTCGTGCCGTTCTTCGGCATCCCCACCTGCACGCTGACCGCGGTGG
GCCGGCTGGCGAAGGCCGGCCGCGCGCAGGTCGTGCCGTTCATCGGCGAGGTGCTGCCGAACTACAAGGGCTATCGGCTG
CGCGTGTTCAAGCCCTGGGAGCAATACCCGACCGGCGACGACGACCTCGACGCGCGCCGCATGAACGCGTTCCTCGAGGA
GCAGATCCCGCTGCTGCCCGAGCAGTATTACTGGGTGCACAAGCGCTTCAAGACGCGTCCGGCCGGCGAGCCGGGCTTTT
ACTGA

Upstream 100 bases:

>100_bases
TTCCCGTCTTGTCACGCAAGGGTGCATTCGTGGTTGCCCACCGCTACGTCCTGAATTTCCCCCTCACGGCACGGGCCTTC
CGGCCCCATGGAGGCCGCTC

Downstream 100 bases:

>100_bases
TCGTGATCGCGGGCGCGGCGGTGCCGGCTCGGCCGCCGCCGAGGTTCGCAAGCCGCGCCCGGCGCGGCATACAATAACGC
CCATGAAACTTCCGTTCACC

Product: lipid A biosynthesis lauroyl acyltransferase

Products: KDO2-(lauroyl)-lipid IVA [C]

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MLGRLGTRFALLLLKLFALLPYGLTARFGDALGWLLYRIPSRRKRIVHTNLKLCFPEWSEARREEVAGRHFRHAIRSYVE
RSVQWFGSEKKLARLIEVDSAIDLTDPDLPPTLFLGLHFVGIEAGSIWLNRSLQRTCGSLYQPFSNPLLEAEAKRARARF
DAEMASRADSARIVLRWLRERKPVMLGADMDYGTRNSTFVPFFGIPTCTLTAVGRLAKAGRAQVVPFIGEVLPNYKGYRL
RVFKPWEQYPTGDDDLDARRMNAFLEEQIPLLPEQYYWVHKRFKTRPAGEPGFY

Sequences:

>Translated_294_residues
MLGRLGTRFALLLLKLFALLPYGLTARFGDALGWLLYRIPSRRKRIVHTNLKLCFPEWSEARREEVAGRHFRHAIRSYVE
RSVQWFGSEKKLARLIEVDSAIDLTDPDLPPTLFLGLHFVGIEAGSIWLNRSLQRTCGSLYQPFSNPLLEAEAKRARARF
DAEMASRADSARIVLRWLRERKPVMLGADMDYGTRNSTFVPFFGIPTCTLTAVGRLAKAGRAQVVPFIGEVLPNYKGYRL
RVFKPWEQYPTGDDDLDARRMNAFLEEQIPLLPEQYYWVHKRFKTRPAGEPGFY
>Mature_294_residues
MLGRLGTRFALLLLKLFALLPYGLTARFGDALGWLLYRIPSRRKRIVHTNLKLCFPEWSEARREEVAGRHFRHAIRSYVE
RSVQWFGSEKKLARLIEVDSAIDLTDPDLPPTLFLGLHFVGIEAGSIWLNRSLQRTCGSLYQPFSNPLLEAEAKRARARF
DAEMASRADSARIVLRWLRERKPVMLGADMDYGTRNSTFVPFFGIPTCTLTAVGRLAKAGRAQVVPFIGEVLPNYKGYRL
RVFKPWEQYPTGDDDLDARRMNAFLEEQIPLLPEQYYWVHKRFKTRPAGEPGFY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=291, Percent_Identity=34.7079037800687, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI87082094, Length=291, Percent_Identity=31.2714776632302, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1788161, Length=261, Percent_Identity=31.0344827586207, Blast_Score=95, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 33881; Mature: 33881

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGRLGTRFALLLLKLFALLPYGLTARFGDALGWLLYRIPSRRKRIVHTNLKLCFPEWSE
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCCHHH
ARREEVAGRHFRHAIRSYVERSVQWFGSEKKLARLIEVDSAIDLTDPDLPPTLFLGLHFV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHE
GIEAGSIWLNRSLQRTCGSLYQPFSNPLLEAEAKRARARFDAEMASRADSARIVLRWLRE
EEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RKPVMLGADMDYGTRNSTFVPFFGIPTCTLTAVGRLAKAGRAQVVPFIGEVLPNYKGYRL
CCCEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEE
RVFKPWEQYPTGDDDLDARRMNAFLEEQIPLLPEQYYWVHKRFKTRPAGEPGFY
EEECCHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLGRLGTRFALLLLKLFALLPYGLTARFGDALGWLLYRIPSRRKRIVHTNLKLCFPEWSE
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCCHHH
ARREEVAGRHFRHAIRSYVERSVQWFGSEKKLARLIEVDSAIDLTDPDLPPTLFLGLHFV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHE
GIEAGSIWLNRSLQRTCGSLYQPFSNPLLEAEAKRARARFDAEMASRADSARIVLRWLRE
EEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RKPVMLGADMDYGTRNSTFVPFFGIPTCTLTAVGRLAKAGRAQVVPFIGEVLPNYKGYRL
CCCEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEE
RVFKPWEQYPTGDDDLDARRMNAFLEEQIPLLPEQYYWVHKRFKTRPAGEPGFY
EEECCHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-lipid IVA; lauroyl-ACP [C]

Specific reaction: KDO2-lipid IVA + lauroyl-ACP = KDO2-(lauroyl)-lipid IVA + [acyl carrier protein] [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]