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The map label for this gene is citA [H]

Identifier: 238028871

GI number: 238028871

Start: 3795386

End: 3796729

Strand: Reverse

Name: citA [H]

Synonym: bglu_1g33360

Alternate gene names: 238028871

Gene position: 3796729-3795386 (Counterclockwise)

Preceding gene: 238028872

Following gene: 238028870

Centisome position: 97.19

GC content: 71.8

Gene sequence:

>1344_bases
ATGGCGTGCACGGCGCAGACGCAACCGGGCGGGGCTGGCAAACGCATGACGACATGGGCGACGGATTCGACGCAACCGCA
GGCGCGCGCACCGGGGCGCCGCGCGGTGATCGCCGCGGTGATCGGCAACGCGCTCGAGTGGTACGACTTCACCGTGTTCA
GCTTCATGGTGGTGGTGATCGCCGACCTGTTCTTCCCGGCCACCAGCGAATACGCGTCGCTGCTGCTGACCACGGCCAGC
TTCGGCGTGGCGTTCTTCATGCGTCCGGTCGGCGGCATCGTGCTGGGCCTCTACGCCGACCGCGCGGGCCGCAGGGCCGC
GCTCTCGCTGGTGATGGTGCTGATGACGCTCGGCATCTTCCTGATCGCGGCCGCGCCGCCCTACGCGGCGATCGGCATCG
CCGCCCCGCTGCTGATCGTGCTCGGCAGGCTGCTGCAGGGCTTCTCGGCGGGCGGCGAGTTCGGCAGCGCGACGGCGCTG
CTGATCGAGGCGGCGCCGCTCTCGCGCCGCGGCTTCTACGGCAGCTGGCAGATGGCGAGCCAGTCGGCGGCGCTGCTGGT
CGGCGCGCTGGTGGGCGCGGCCGTCACGCACGGGCTGTCGGGCGCCGCGCTGCACGCCTGGGGCTGGCGCGTGCCGTTCC
TGATCGGGATGCTGATCGGCCCGATCGGCTATTACCTGCGCCGCCATCTGGCCGACTCCGAGGCGTTCCTGTTCGCCAAG
GCGCGCGCGCGGCGCGCGACGCTCGGCGAGGTGCTCGCGCAGCACGGCCGCGAGGTGCTCTGCGGGCTCGGCTCGGTGGT
CGCGCTGACGGTGACGGTCTACGTGCTGCTCAGCTATCTGCCGACCTTCGCCGTCAAGCAGCTGAAGCTGCCCTACGGCG
AGTCGTTCTACGCGACGATCGTGGGCGGCGTGCTGCTGACCGTGCTCTCGCCGGTGGCGGGCGCCTGGTCGGACCGCCTC
GGGCGCAAGCGGCTGTCGCTCGCCTCGCTGGTGGCGACGCTGGTCCTGATCTATCCGCTCTTTCTCTGGCTGGACGCGGC
GCCGAGCATCGGCCGGCTGGTGCTCGTGCAGGGCATCCTCGCCGTCACGCTGGCGGGCTACTACGGGCCGTTCGGCGCGA
TGATCGCCGAGCGGTTCCCGGCCAACGTGCGCTCCACCGGGCTGTCGCTTGCTTACAACGTCGCGGTGATGCTGTTCGGC
GGGTTCGGGCAGACCATCGTCACCTGGCTGATCCGCGCCACGGGCACGCCGCTTGCGCCGACCTACTACGTGATGGCGGG
CCTCGCGCTGTCGGTGGTCGCGATCGCCGCGATGCCGCGCCTCGCGCCCGGCGCCGAGGGCTGA

Upstream 100 bases:

>100_bases
AACGCCGGCCGTTTTTTTGGTGCGGCTTGTGTTCGCCGGCGGCGCTCCCTACACTCGCTGCACGATGCGCGCCGCACGGC
GCGCACGGGGCACGGCGGGC

Downstream 100 bases:

>100_bases
GCCGCGCAGCGGGCCGCGGCGCAGCCGCCGGTGCCGCCGGCCCGCGCGTGCCGTGCCGCAGCCGCCGCGCGGCGCGGGCC
TCGCGTCGGGCATTTTGCGG

Product: major facilitator superfamily transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MACTAQTQPGGAGKRMTTWATDSTQPQARAPGRRAVIAAVIGNALEWYDFTVFSFMVVVIADLFFPATSEYASLLLTTAS
FGVAFFMRPVGGIVLGLYADRAGRRAALSLVMVLMTLGIFLIAAAPPYAAIGIAAPLLIVLGRLLQGFSAGGEFGSATAL
LIEAAPLSRRGFYGSWQMASQSAALLVGALVGAAVTHGLSGAALHAWGWRVPFLIGMLIGPIGYYLRRHLADSEAFLFAK
ARARRATLGEVLAQHGREVLCGLGSVVALTVTVYVLLSYLPTFAVKQLKLPYGESFYATIVGGVLLTVLSPVAGAWSDRL
GRKRLSLASLVATLVLIYPLFLWLDAAPSIGRLVLVQGILAVTLAGYYGPFGAMIAERFPANVRSTGLSLAYNVAVMLFG
GFGQTIVTWLIRATGTPLAPTYYVMAGLALSVVAIAAMPRLAPGAEG

Sequences:

>Translated_447_residues
MACTAQTQPGGAGKRMTTWATDSTQPQARAPGRRAVIAAVIGNALEWYDFTVFSFMVVVIADLFFPATSEYASLLLTTAS
FGVAFFMRPVGGIVLGLYADRAGRRAALSLVMVLMTLGIFLIAAAPPYAAIGIAAPLLIVLGRLLQGFSAGGEFGSATAL
LIEAAPLSRRGFYGSWQMASQSAALLVGALVGAAVTHGLSGAALHAWGWRVPFLIGMLIGPIGYYLRRHLADSEAFLFAK
ARARRATLGEVLAQHGREVLCGLGSVVALTVTVYVLLSYLPTFAVKQLKLPYGESFYATIVGGVLLTVLSPVAGAWSDRL
GRKRLSLASLVATLVLIYPLFLWLDAAPSIGRLVLVQGILAVTLAGYYGPFGAMIAERFPANVRSTGLSLAYNVAVMLFG
GFGQTIVTWLIRATGTPLAPTYYVMAGLALSVVAIAAMPRLAPGAEG
>Mature_446_residues
ACTAQTQPGGAGKRMTTWATDSTQPQARAPGRRAVIAAVIGNALEWYDFTVFSFMVVVIADLFFPATSEYASLLLTTASF
GVAFFMRPVGGIVLGLYADRAGRRAALSLVMVLMTLGIFLIAAAPPYAAIGIAAPLLIVLGRLLQGFSAGGEFGSATALL
IEAAPLSRRGFYGSWQMASQSAALLVGALVGAAVTHGLSGAALHAWGWRVPFLIGMLIGPIGYYLRRHLADSEAFLFAKA
RARRATLGEVLAQHGREVLCGLGSVVALTVTVYVLLSYLPTFAVKQLKLPYGESFYATIVGGVLLTVLSPVAGAWSDRLG
RKRLSLASLVATLVLIYPLFLWLDAAPSIGRLVLVQGILAVTLAGYYGPFGAMIAERFPANVRSTGLSLAYNVAVMLFGG
FGQTIVTWLIRATGTPLAPTYYVMAGLALSVVAIAAMPRLAPGAEG

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=406, Percent_Identity=33.0049261083744, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788942, Length=417, Percent_Identity=31.6546762589928, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1789941, Length=418, Percent_Identity=33.2535885167464, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1788292, Length=426, Percent_Identity=29.1079812206573, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI87082231, Length=440, Percent_Identity=25, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082404, Length=382, Percent_Identity=24.0837696335079, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46940; Mature: 46809

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACTAQTQPGGAGKRMTTWATDSTQPQARAPGRRAVIAAVIGNALEWYDFTVFSFMVVVI
CCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ADLFFPATSEYASLLLTTASFGVAFFMRPVGGIVLGLYADRAGRRAALSLVMVLMTLGIF
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LIAAAPPYAAIGIAAPLLIVLGRLLQGFSAGGEFGSATALLIEAAPLSRRGFYGSWQMAS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEECCCCCCCCCCCCHHHHH
QSAALLVGALVGAAVTHGLSGAALHAWGWRVPFLIGMLIGPIGYYLRRHLADSEAFLFAK
HHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ARARRATLGEVLAQHGREVLCGLGSVVALTVTVYVLLSYLPTFAVKQLKLPYGESFYATI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VGGVLLTVLSPVAGAWSDRLGRKRLSLASLVATLVLIYPLFLWLDAAPSIGRLVLVQGIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AVTLAGYYGPFGAMIAERFPANVRSTGLSLAYNVAVMLFGGFGQTIVTWLIRATGTPLAP
HHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCH
TYYVMAGLALSVVAIAAMPRLAPGAEG
HHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ACTAQTQPGGAGKRMTTWATDSTQPQARAPGRRAVIAAVIGNALEWYDFTVFSFMVVVI
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ADLFFPATSEYASLLLTTASFGVAFFMRPVGGIVLGLYADRAGRRAALSLVMVLMTLGIF
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LIAAAPPYAAIGIAAPLLIVLGRLLQGFSAGGEFGSATALLIEAAPLSRRGFYGSWQMAS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEECCCCCCCCCCCCHHHHH
QSAALLVGALVGAAVTHGLSGAALHAWGWRVPFLIGMLIGPIGYYLRRHLADSEAFLFAK
HHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ARARRATLGEVLAQHGREVLCGLGSVVALTVTVYVLLSYLPTFAVKQLKLPYGESFYATI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VGGVLLTVLSPVAGAWSDRLGRKRLSLASLVATLVLIYPLFLWLDAAPSIGRLVLVQGIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AVTLAGYYGPFGAMIAERFPANVRSTGLSLAYNVAVMLFGGFGQTIVTWLIRATGTPLAP
HHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCH
TYYVMAGLALSVVAIAAMPRLAPGAEG
HHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]