Click here to switch to the map view.

The map label for this gene is slt [H]

Identifier: 238028859

GI number: 238028859

Start: 3781810

End: 3783762

Strand: Reverse

Name: slt [H]

Synonym: bglu_1g33240

Alternate gene names: 238028859

Gene position: 3783762-3781810 (Counterclockwise)

Preceding gene: 238028861

Following gene: 238028858

Centisome position: 96.86

GC content: 70.4

Gene sequence:

>1953_bases
ATGTCAACCAGCCTCGTCCGAGTATATCGCGCGGCCGCCATGACGCTGTCGGCGCTCGCGCTGGCCGTGGGCGCCGCCGC
CTGCGCCGCGCAACCCGTCGACCCGGTCTCGTCGAACGACGACCAGATCTTCGTCCAGCTTCGCGAGGCCGCGCGCGCCA
ACAACGCCGCGCGTGCCGCGCAGCTGGCCGCGATGATTCCCGGCTACCCGGCGCCGTCCTATCTCGAATACTTCCAGATC
AAGCCGCAGCTGTTCGACAGCTCGGGGCGCGCGCGCATCGATGCGCCCGACGCGCCGGTGCTGTCGTTCCTGCAGCGCTA
CGACGGCCAGGCGATCGCCGACCGGATGCGCAACGACTACCTGCTGGTGCTGGGCGCGCGCCACGACTGGCGCGGCTTCG
ACGACCAGTACAAGCGCTTCGTGCTCGACGACGACACCCAGGTCAAGTGCTACGCGCTGGAGTCGCGCGCCGCGCGCGGC
GAGAAGGTGGCCGACATCGCGCGCGACCTGCTGGTCGATCCGAAATACTACGGCGACGGCTGCGTGGATCTGATCTCGGC
GCTCGCCGTGAACCAGCAGTTCTCGTCCGACGACGTCTGGAGGCAGGTGCGGCTCGCGTTCGAGCAGAACTACACCACCA
CCGCCGGCAAGATCGCCGACGTGCTCGGCCCGCGCCCGGTGGCGTTCGAGCAGGCCGCGAGCGCGCCGCCGCTGTTCCTC
GCGCGCGGCGTGGGCGCCGACGCCACCTCGCGCCAGCTCGCGCTGATCGCGATCACGCGCATGGCGCGCAACGATCCGGA
CCAGGCCGCGGCGATGCTCGCCACGCTCGGTACGCTGTCGAAGCCCGAGCAGGCGATCGCCTGGGGCGAGATCGGCATGC
AGGCGGCGATCAAGCGCCTGCCGCTGGCGGTGCAGTATTACAAGCAGTCGGGCGGCGCGCCGCTGTCGACGCCGGGCTAC
GAATGGCGCGTGCGCGCGGCGCTGCTCGCGGGCGACTGGCCGATGGTGCGCTGGGCGATCGAGCAGATGCCGGCCGACCT
GCGCGCGCAGCCGGCCTGGCTCTACTGGCATGGCCGCGCGCTGAAGCAGGCCGGCGACGCCGGCGGCGCGAGCCGCGATT
TCGAGCAGGTGGCGGGCCGCTTCGACTTCTACGGCCAGCTCGCGGCCGAGGAGCTCGGCCAGAAGACCGTGATCCCGCCG
CGCACCACCGTGACCGACGCCGAGGTGGAGGCGATGAGCCAGGTGCCGGGCTTCGCGCTGGCACGGCGCTTCTATGCGCT
GAACCTGCGGCTCGAGGGCAACCGCGAGTGGAACTGGCCGCTGCGCGGCATGACCGACCGCCAGCTGCTGGCCGCGGCCG
AATACGCGAAGCGCATCGACCTGCTCGACCGCACCGTCAACACCGCCGACCGCACCACCGCGCTGCACGACTTCACGCTG
CGCTACCCCGCGCCGTACCGGCCGATCGTGGAGCGCTACGCGCAGACCAACGGCCTCGACATCGAATGGGCCTACGGCCT
GATCCGCCAGGAGTCGCGCTTCATCACCAACGCGCGTTCCTCGGTCGGCGCGGGCGGCCTGATGCAACTGATGCCGGCCA
CCGCGCAGCTGGTCGCCAAGAAGCTCGGCATGGGCGGCATCTCGCGCGAGCAGATGCACGACATCGACACCAACATCCAG
CTCGGCACCTGGTATCTGTCCGACATCTACCAGAAGTTCGACGACTCGGCCGTGCTCGCCACGGCCGGCTACAACGCCGG
CCCGGGCCGGCCGCGCCAGTGGCGGCAGGCGCTGACGAAGCCGGTGGAGGGCGCGATCTTCGCCGAGACGATCCCGTTCA
ACGAGACGCGCGACTACGTGGAGAACGTGCTGTCGAACACCACTTATTACGCGGCGCTGTTCGAAGGCAAGCCCGAATCG
CTGAAGGCGCGCCTCGGCTTCATCGCGCCCTGA

Upstream 100 bases:

>100_bases
GCGCGAGCGTTTCGCGCAGCGCCTGCTTGGCGATGGCGGCGGGGGGGCGTGCTATGCTTTCGCTCACTTCGTGCTCCGGT
TCCGTTCCCTGTTCAAATCG

Downstream 100 bases:

>100_bases
ACCACGCGCACCGGCGGCCCGCGCCGGGCCGCCCGGTGCCGCGCCCGATGCCCTTGCGCTGCGCGCGGCCCGGCCGGCGG
CGCGTTTTCGACACCTGACG

Product: putative transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 650; Mature: 649

Protein sequence:

>650_residues
MSTSLVRVYRAAAMTLSALALAVGAAACAAQPVDPVSSNDDQIFVQLREAARANNAARAAQLAAMIPGYPAPSYLEYFQI
KPQLFDSSGRARIDAPDAPVLSFLQRYDGQAIADRMRNDYLLVLGARHDWRGFDDQYKRFVLDDDTQVKCYALESRAARG
EKVADIARDLLVDPKYYGDGCVDLISALAVNQQFSSDDVWRQVRLAFEQNYTTTAGKIADVLGPRPVAFEQAASAPPLFL
ARGVGADATSRQLALIAITRMARNDPDQAAAMLATLGTLSKPEQAIAWGEIGMQAAIKRLPLAVQYYKQSGGAPLSTPGY
EWRVRAALLAGDWPMVRWAIEQMPADLRAQPAWLYWHGRALKQAGDAGGASRDFEQVAGRFDFYGQLAAEELGQKTVIPP
RTTVTDAEVEAMSQVPGFALARRFYALNLRLEGNREWNWPLRGMTDRQLLAAAEYAKRIDLLDRTVNTADRTTALHDFTL
RYPAPYRPIVERYAQTNGLDIEWAYGLIRQESRFITNARSSVGAGGLMQLMPATAQLVAKKLGMGGISREQMHDIDTNIQ
LGTWYLSDIYQKFDDSAVLATAGYNAGPGRPRQWRQALTKPVEGAIFAETIPFNETRDYVENVLSNTTYYAALFEGKPES
LKARLGFIAP

Sequences:

>Translated_650_residues
MSTSLVRVYRAAAMTLSALALAVGAAACAAQPVDPVSSNDDQIFVQLREAARANNAARAAQLAAMIPGYPAPSYLEYFQI
KPQLFDSSGRARIDAPDAPVLSFLQRYDGQAIADRMRNDYLLVLGARHDWRGFDDQYKRFVLDDDTQVKCYALESRAARG
EKVADIARDLLVDPKYYGDGCVDLISALAVNQQFSSDDVWRQVRLAFEQNYTTTAGKIADVLGPRPVAFEQAASAPPLFL
ARGVGADATSRQLALIAITRMARNDPDQAAAMLATLGTLSKPEQAIAWGEIGMQAAIKRLPLAVQYYKQSGGAPLSTPGY
EWRVRAALLAGDWPMVRWAIEQMPADLRAQPAWLYWHGRALKQAGDAGGASRDFEQVAGRFDFYGQLAAEELGQKTVIPP
RTTVTDAEVEAMSQVPGFALARRFYALNLRLEGNREWNWPLRGMTDRQLLAAAEYAKRIDLLDRTVNTADRTTALHDFTL
RYPAPYRPIVERYAQTNGLDIEWAYGLIRQESRFITNARSSVGAGGLMQLMPATAQLVAKKLGMGGISREQMHDIDTNIQ
LGTWYLSDIYQKFDDSAVLATAGYNAGPGRPRQWRQALTKPVEGAIFAETIPFNETRDYVENVLSNTTYYAALFEGKPES
LKARLGFIAP
>Mature_649_residues
STSLVRVYRAAAMTLSALALAVGAAACAAQPVDPVSSNDDQIFVQLREAARANNAARAAQLAAMIPGYPAPSYLEYFQIK
PQLFDSSGRARIDAPDAPVLSFLQRYDGQAIADRMRNDYLLVLGARHDWRGFDDQYKRFVLDDDTQVKCYALESRAARGE
KVADIARDLLVDPKYYGDGCVDLISALAVNQQFSSDDVWRQVRLAFEQNYTTTAGKIADVLGPRPVAFEQAASAPPLFLA
RGVGADATSRQLALIAITRMARNDPDQAAAMLATLGTLSKPEQAIAWGEIGMQAAIKRLPLAVQYYKQSGGAPLSTPGYE
WRVRAALLAGDWPMVRWAIEQMPADLRAQPAWLYWHGRALKQAGDAGGASRDFEQVAGRFDFYGQLAAEELGQKTVIPPR
TTVTDAEVEAMSQVPGFALARRFYALNLRLEGNREWNWPLRGMTDRQLLAAAEYAKRIDLLDRTVNTADRTTALHDFTLR
YPAPYRPIVERYAQTNGLDIEWAYGLIRQESRFITNARSSVGAGGLMQLMPATAQLVAKKLGMGGISREQMHDIDTNIQL
GTWYLSDIYQKFDDSAVLATAGYNAGPGRPRQWRQALTKPVEGAIFAETIPFNETRDYVENVLSNTTYYAALFEGKPESL
KARLGFIAP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=643, Percent_Identity=26.1275272161742, Blast_Score=193, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 71732; Mature: 71601

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSLVRVYRAAAMTLSALALAVGAAACAAQPVDPVSSNDDQIFVQLREAARANNAARAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHH
QLAAMIPGYPAPSYLEYFQIKPQLFDSSGRARIDAPDAPVLSFLQRYDGQAIADRMRNDY
HHHHHCCCCCCCHHHHHHCCCHHHCCCCCCEEECCCCCHHHHHHHHHCCHHHHHHHCCCE
LLVLGARHDWRGFDDQYKRFVLDDDTQVKCYALESRAARGEKVADIARDLLVDPKYYGDG
EEEEECCCCCCCCCHHHHEEEECCCCCEEEEEECHHHCCCHHHHHHHHHHHCCCCCCCCH
CVDLISALAVNQQFSSDDVWRQVRLAFEQNYTTTAGKIADVLGPRPVAFEQAASAPPLFL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHCCCCCEEE
ARGVGADATSRQLALIAITRMARNDPDQAAAMLATLGTLSKPEQAIAWGEIGMQAAIKRL
ECCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
PLAVQYYKQSGGAPLSTPGYEWRVRAALLAGDWPMVRWAIEQMPADLRAQPAWLYWHGRA
CHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEECHH
LKQAGDAGGASRDFEQVAGRFDFYGQLAAEELGQKTVIPPRTTVTDAEVEAMSQVPGFAL
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHHH
ARRFYALNLRLEGNREWNWPLRGMTDRQLLAAAEYAKRIDLLDRTVNTADRTTALHDFTL
HHHEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEE
RYPAPYRPIVERYAQTNGLDIEWAYGLIRQESRFITNARSSVGAGGLMQLMPATAQLVAK
ECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
KLGMGGISREQMHDIDTNIQLGTWYLSDIYQKFDDSAVLATAGYNAGPGRPRQWRQALTK
HHCCCCCCHHHHHHCCCCEEEEHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHCC
PVEGAIFAETIPFNETRDYVENVLSNTTYYAALFEGKPESLKARLGFIAP
CCCCCEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCCCC
>Mature Secondary Structure 
STSLVRVYRAAAMTLSALALAVGAAACAAQPVDPVSSNDDQIFVQLREAARANNAARAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHH
QLAAMIPGYPAPSYLEYFQIKPQLFDSSGRARIDAPDAPVLSFLQRYDGQAIADRMRNDY
HHHHHCCCCCCCHHHHHHCCCHHHCCCCCCEEECCCCCHHHHHHHHHCCHHHHHHHCCCE
LLVLGARHDWRGFDDQYKRFVLDDDTQVKCYALESRAARGEKVADIARDLLVDPKYYGDG
EEEEECCCCCCCCCHHHHEEEECCCCCEEEEEECHHHCCCHHHHHHHHHHHCCCCCCCCH
CVDLISALAVNQQFSSDDVWRQVRLAFEQNYTTTAGKIADVLGPRPVAFEQAASAPPLFL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHCCCCCEEE
ARGVGADATSRQLALIAITRMARNDPDQAAAMLATLGTLSKPEQAIAWGEIGMQAAIKRL
ECCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
PLAVQYYKQSGGAPLSTPGYEWRVRAALLAGDWPMVRWAIEQMPADLRAQPAWLYWHGRA
CHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEECHH
LKQAGDAGGASRDFEQVAGRFDFYGQLAAEELGQKTVIPPRTTVTDAEVEAMSQVPGFAL
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCHHHH
ARRFYALNLRLEGNREWNWPLRGMTDRQLLAAAEYAKRIDLLDRTVNTADRTTALHDFTL
HHHEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEE
RYPAPYRPIVERYAQTNGLDIEWAYGLIRQESRFITNARSSVGAGGLMQLMPATAQLVAK
ECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
KLGMGGISREQMHDIDTNIQLGTWYLSDIYQKFDDSAVLATAGYNAGPGRPRQWRQALTK
HHCCCCCCHHHHHHCCCCEEEEHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHCC
PVEGAIFAETIPFNETRDYVENVLSNTTYYAALFEGKPESLKARLGFIAP
CCCCCEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]