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The map label for this gene is dctD [H]

Identifier: 238028729

GI number: 238028729

Start: 3625576

End: 3626943

Strand: Reverse

Name: dctD [H]

Synonym: bglu_1g31930

Alternate gene names: 238028729

Gene position: 3626943-3625576 (Counterclockwise)

Preceding gene: 238028730

Following gene: 238028727

Centisome position: 92.84

GC content: 71.64

Gene sequence:

>1368_bases
ATGGCGAACCGCCTGCAGGTGATTTATATCGAAGACGACGAACTGGTGCGACGCGCGGCCGTGCAGAGCCTGCAGCTCGC
CGGTTTCGAGGTGGCCGGCTTCGGCTCGGCCGAAGCGGCCGAGAAGGCGATCCTCGGCGAGCGCGTCGGCGCCGTGGTGT
CCGACATCCGGCTGCCGGGGGCAAGCGGCCTCGAACTGCTCGCGCAGTGCCGCGAGCGCGCGCCGGACGTGCCGGTGATC
CTCGTCACCGGCCACGGCGACATCAGCATGGCGGTGCAGGCGATGCGCGACGGCGCCTACGACTTCATCGAGAAGCCGTT
CGCCTCCGAGCGGTTGATCGAGACGGTGCGCCGCGCGATCGAGCGGCGCGAGCTGGTGCTCGAGAATCTCGCGCTGCGCC
GCGAGCTGGCCGTGCAGGGCCTGGTCGCGCCGCGCATCATCGGCCGCAGCCCGGCGATCGAGCAGGTGCGGCGGCTGATC
GCCAACGTCGCGCCGACCGACGCGTCGGTGCTGATCAACGGCGATACCGGCGCCGGCAAGGAACTGATCGCGCGCAGCCT
GCACGAGATGTCGCCGCGCCGCGACAAGCCGTTCATTGCCGTGAACTGCGGAGCGCTGCCCGAGCCGATGTTCGAGTCGG
AGATGTTCGGCTACGAGCCCGGGGCGTTCACGGGCGCCGCCAAGCGCCGCGTCGGCAAGCTCGAGTACGCCTCGGGCGGC
ACGCTGTTCCTCGACGAGATCGAGAGCATGCCGCTCGCGCTGCAGGTCAAGCTGCTGCGCGTGCTGCAGGACGGCGTGCT
GGAGCGGCTCGGCTCGAACCAGCCGATTCGCGTGAACTGCCGGGTGGTGGCCGCCGCCAAGGGCGACATGGTCGAGCACG
TGGCGGCCGGCACGTTCCGGCGCGACCTGCTGTACCGCCTCAACGTCGTCACCATCGCGCTGCCGCCGCTGGCCGAGCGG
CGCGAGGACATCGTGCCGCTGTTCGAGCACTTCATGCTCGACGCGGCCGTGCGCTACGGGCGCCCCGCGCCGCACCTGAC
CGACCGCCAGCGCGAGAGCCTGATGCAGCGCGACTGGCCCGGCAACGTGCGCGAACTGCGCAACGCCGCCGACCGCTTCG
TGCTGGGCGTGGCCGAAGGGCTGGCCGATGCCGGCGCGGGCGCCGCCGAGGCGCGCGACGAGGCGGGCGCCGAGCAGTCG
CTGAAGGAGCGCGTCGAGCAGTTCGAGCGCGCCGTGATCGCGGACACCCTGACGCAGACAGGCGGCGCCGTCGCCGCCAC
CGCCGACCGGCTGCACGTGGGCAAGGCCACGCTCTACGAGAAGATCAAGCGCTACGGGCTGTCGACCAAGGGCGAGCACG
AACGCTGA

Upstream 100 bases:

>100_bases
ACGCTGCGCCGCGCGTACACGCATCATCCGCAGACGATGTCCGCCGGCCGTTGACGGATCGCGCCGGCCGCGGCATCGAC
AGCAATCCGCGTGAGGGAAG

Downstream 100 bases:

>100_bases
GGCGGCGCGCGCCGCCTGGATGGACGAAGGCCGGCGGCGCCACGCGGCGCGGCCGGCCTTGGCAAGGCGGCGCGCTTACG
CGTTCGTGGCGAGCGCCTTC

Product: C4-dicarboxylate transport transcriptional regulator DctD

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MANRLQVIYIEDDELVRRAAVQSLQLAGFEVAGFGSAEAAEKAILGERVGAVVSDIRLPGASGLELLAQCRERAPDVPVI
LVTGHGDISMAVQAMRDGAYDFIEKPFASERLIETVRRAIERRELVLENLALRRELAVQGLVAPRIIGRSPAIEQVRRLI
ANVAPTDASVLINGDTGAGKELIARSLHEMSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRVGKLEYASGG
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMVEHVAAGTFRRDLLYRLNVVTIALPPLAER
REDIVPLFEHFMLDAAVRYGRPAPHLTDRQRESLMQRDWPGNVRELRNAADRFVLGVAEGLADAGAGAAEARDEAGAEQS
LKERVEQFERAVIADTLTQTGGAVAATADRLHVGKATLYEKIKRYGLSTKGEHER

Sequences:

>Translated_455_residues
MANRLQVIYIEDDELVRRAAVQSLQLAGFEVAGFGSAEAAEKAILGERVGAVVSDIRLPGASGLELLAQCRERAPDVPVI
LVTGHGDISMAVQAMRDGAYDFIEKPFASERLIETVRRAIERRELVLENLALRRELAVQGLVAPRIIGRSPAIEQVRRLI
ANVAPTDASVLINGDTGAGKELIARSLHEMSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRVGKLEYASGG
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMVEHVAAGTFRRDLLYRLNVVTIALPPLAER
REDIVPLFEHFMLDAAVRYGRPAPHLTDRQRESLMQRDWPGNVRELRNAADRFVLGVAEGLADAGAGAAEARDEAGAEQS
LKERVEQFERAVIADTLTQTGGAVAATADRLHVGKATLYEKIKRYGLSTKGEHER
>Mature_454_residues
ANRLQVIYIEDDELVRRAAVQSLQLAGFEVAGFGSAEAAEKAILGERVGAVVSDIRLPGASGLELLAQCRERAPDVPVIL
VTGHGDISMAVQAMRDGAYDFIEKPFASERLIETVRRAIERRELVLENLALRRELAVQGLVAPRIIGRSPAIEQVRRLIA
NVAPTDASVLINGDTGAGKELIARSLHEMSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRVGKLEYASGGT
LFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMVEHVAAGTFRRDLLYRLNVVTIALPPLAERR
EDIVPLFEHFMLDAAVRYGRPAPHLTDRQRESLMQRDWPGNVRELRNAADRFVLGVAEGLADAGAGAAEARDEAGAEQSL
KERVEQFERAVIADTLTQTGGAVAATADRLHVGKATLYEKIKRYGLSTKGEHER

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=38.4615384615385, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1788550, Length=452, Percent_Identity=37.6106194690265, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1790437, Length=443, Percent_Identity=37.6975169300226, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI1788905, Length=443, Percent_Identity=37.6975169300226, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=40, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI87082117, Length=321, Percent_Identity=42.0560747663551, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789087, Length=340, Percent_Identity=42.0588235294118, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87082152, Length=318, Percent_Identity=42.4528301886792, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI87081872, Length=230, Percent_Identity=47.3913043478261, Blast_Score=214, Evalue=8e-57,
Organism=Escherichia coli, GI1786524, Length=249, Percent_Identity=46.5863453815261, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI1787583, Length=317, Percent_Identity=38.4858044164038, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI87081858, Length=302, Percent_Identity=30.794701986755, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789828, Length=244, Percent_Identity=34.4262295081967, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1790552, Length=145, Percent_Identity=28.2758620689655, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789402, Length=121, Percent_Identity=33.0578512396694, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=27.6422764227642, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49707; Mature: 49576

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRLQVIYIEDDELVRRAAVQSLQLAGFEVAGFGSAEAAEKAILGERVGAVVSDIRLPG
CCCCEEEEEECCHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
ASGLELLAQCRERAPDVPVILVTGHGDISMAVQAMRDGAYDFIEKPFASERLIETVRRAI
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHH
ERRELVLENLALRRELAVQGLVAPRIIGRSPAIEQVRRLIANVAPTDASVLINGDTGAGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
ELIARSLHEMSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRVGKLEYASGG
HHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHCCEEECCCC
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMVEHVAAGTFR
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHHH
RDLLYRLNVVTIALPPLAERREDIVPLFEHFMLDAAVRYGRPAPHLTDRQRESLMQRDWP
HHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
GNVRELRNAADRFVLGVAEGLADAGAGAAEARDEAGAEQSLKERVEQFERAVIADTLTQT
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
GGAVAATADRLHVGKATLYEKIKRYGLSTKGEHER
CCCEEECHHHHHCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ANRLQVIYIEDDELVRRAAVQSLQLAGFEVAGFGSAEAAEKAILGERVGAVVSDIRLPG
CCCEEEEEECCHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
ASGLELLAQCRERAPDVPVILVTGHGDISMAVQAMRDGAYDFIEKPFASERLIETVRRAI
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHH
ERRELVLENLALRRELAVQGLVAPRIIGRSPAIEQVRRLIANVAPTDASVLINGDTGAGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
ELIARSLHEMSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRVGKLEYASGG
HHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHCCEEECCCC
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMVEHVAAGTFR
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHHH
RDLLYRLNVVTIALPPLAERREDIVPLFEHFMLDAAVRYGRPAPHLTDRQRESLMQRDWP
HHHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
GNVRELRNAADRFVLGVAEGLADAGAGAAEARDEAGAEQSLKERVEQFERAVIADTLTQT
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHC
GGAVAATADRLHVGKATLYEKIKRYGLSTKGEHER
CCCEEECHHHHHCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]