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The map label for this gene is purU [H]

Identifier: 238028647

GI number: 238028647

Start: 3540141

End: 3541022

Strand: Reverse

Name: purU [H]

Synonym: bglu_1g31110

Alternate gene names: 238028647

Gene position: 3541022-3540141 (Counterclockwise)

Preceding gene: 238028648

Following gene: 238028644

Centisome position: 90.64

GC content: 65.42

Gene sequence:

>882_bases
ATGTCGACCGACCATAGCTTTATCCTGAAACTGTCATGTCCCGACAAGCACGGCATCGTGCATGCCGTGTCGGGTTTCCT
GTTCGAGCGCGATACCAACATCGTCGATTCCGCGCAGTTCGGCGACAGCCGCACCGGCGAGTTCTTCATGCGCGTGCATT
TCGACCAGGCCAGCGCGGCCGATGCCGGCACCGCGCTCGACACGCTGCGCGACGCGTTCGCGCCGCTCGCCGAACGCTTC
GCGATGCGCTGGGAGCTGCACGACGCGTCGATCAAGCCGCGCGTGGTGATCCTCGTCTCGAAGATCGGCCACTGCCTGAA
CGACCTGCTGTTCCGCTACCGCACCGGTCAGCTGCCGATCGAGATCGCGGCGATCGTCTCGAACCACAAGGATTTCTACC
AGCTCGCCGCCAGCTACGACGTGCCGTTCCACCATTTCCCGCTGGTCGCGGGTGCCTCGGCGCAGGCGAAGGCCGCGCAG
GAGGCGCGCGTGCTGGAGGTGATCGACGAGCATTCGGCCGACCTGGTGGTGCTCGCGCGCTACATGCAGATCCTGTCGCA
GGACATGTGCCGGCGCCTGGCCGGCCGCGCGATCAATATCCACCATTCGTTCCTGCCGAGCTTCAAGGGCGCGAAGCCGT
ATTACCAGGCGTTCGACCGCGGCGTGAAGCTGATCGGCGCGACCGCGCACTACGTGACGACCGACCTCGACGAAGGCCCG
ATCATCGAGCAGGAAGTCGAGCGCGTCGACCATAGCATGACGCCGGAGCAGCTGACCGCGATCGGCCGCGACGTCGAATG
CGTGACGCTCGCGCGCGCCGTGAAATGGCACGTCGAGCACCGCATCCTGCAGAACGGCACGAAGACGGTGGTGTTCCGCT
GA

Upstream 100 bases:

>100_bases
CTCGCGCCGCAGGCGGCGGCCGGCATCGACGCGCTGTTCTCGCCGCCGCCGGCGGCCTGACGGCGCCACGAATTCAATCG
ATTGGAATGGAGGTGCGGTC

Downstream 100 bases:

>100_bases
AGCCGGCAGCGGGCGAGGGCCGGCCGCGCGGCCGCGCCCGCGCCTCTGCTCAGATGAGGCGCAGCGTTGCCAGCTCGCGC
TTCAGGTAGGCATAGAAGAT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSTDHSFILKLSCPDKHGIVHAVSGFLFERDTNIVDSAQFGDSRTGEFFMRVHFDQASAADAGTALDTLRDAFAPLAERF
AMRWELHDASIKPRVVILVSKIGHCLNDLLFRYRTGQLPIEIAAIVSNHKDFYQLAASYDVPFHHFPLVAGASAQAKAAQ
EARVLEVIDEHSADLVVLARYMQILSQDMCRRLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGP
IIEQEVERVDHSMTPEQLTAIGRDVECVTLARAVKWHVEHRILQNGTKTVVFR

Sequences:

>Translated_293_residues
MSTDHSFILKLSCPDKHGIVHAVSGFLFERDTNIVDSAQFGDSRTGEFFMRVHFDQASAADAGTALDTLRDAFAPLAERF
AMRWELHDASIKPRVVILVSKIGHCLNDLLFRYRTGQLPIEIAAIVSNHKDFYQLAASYDVPFHHFPLVAGASAQAKAAQ
EARVLEVIDEHSADLVVLARYMQILSQDMCRRLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGP
IIEQEVERVDHSMTPEQLTAIGRDVECVTLARAVKWHVEHRILQNGTKTVVFR
>Mature_292_residues
STDHSFILKLSCPDKHGIVHAVSGFLFERDTNIVDSAQFGDSRTGEFFMRVHFDQASAADAGTALDTLRDAFAPLAERFA
MRWELHDASIKPRVVILVSKIGHCLNDLLFRYRTGQLPIEIAAIVSNHKDFYQLAASYDVPFHHFPLVAGASAQAKAAQE
ARVLEVIDEHSADLVVLARYMQILSQDMCRRLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGPI
IEQEVERVDHSMTPEQLTAIGRDVECVTLARAVKWHVEHRILQNGTKTVVFR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=182, Percent_Identity=32.4175824175824, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI209869995, Length=182, Percent_Identity=32.4175824175824, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI209869993, Length=182, Percent_Identity=32.4175824175824, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787483, Length=285, Percent_Identity=39.6491228070175, Blast_Score=217, Evalue=9e-58,
Organism=Escherichia coli, GI1788846, Length=189, Percent_Identity=29.1005291005291, Blast_Score=85, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=167, Percent_Identity=34.1317365269461, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32809; Mature: 32678

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDHSFILKLSCPDKHGIVHAVSGFLFERDTNIVDSAQFGDSRTGEFFMRVHFDQASAA
CCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCC
DAGTALDTLRDAFAPLAERFAMRWELHDASIKPRVVILVSKIGHCLNDLLFRYRTGQLPI
CCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCE
EIAAIVSNHKDFYQLAASYDVPFHHFPLVAGASAQAKAAQEARVLEVIDEHSADLVVLAR
EEHHHHHCCHHHHHHHHHCCCCCHHCCEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
YMQILSQDMCRRLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGP
HHHHHHHHHHHHHHCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEEECCCCCC
IIEQEVERVDHSMTPEQLTAIGRDVECVTLARAVKWHVEHRILQNGTKTVVFR
CCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure 
STDHSFILKLSCPDKHGIVHAVSGFLFERDTNIVDSAQFGDSRTGEFFMRVHFDQASAA
CCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCC
DAGTALDTLRDAFAPLAERFAMRWELHDASIKPRVVILVSKIGHCLNDLLFRYRTGQLPI
CCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCE
EIAAIVSNHKDFYQLAASYDVPFHHFPLVAGASAQAKAAQEARVLEVIDEHSADLVVLAR
EEHHHHHCCHHHHHHHHHCCCCCHHCCEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
YMQILSQDMCRRLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGP
HHHHHHHHHHHHHHCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEEECCCCCC
IIEQEVERVDHSMTPEQLTAIGRDVECVTLARAVKWHVEHRILQNGTKTVVFR
CCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]