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The map label for this gene is ycdT [H]

Identifier: 238028556

GI number: 238028556

Start: 3427513

End: 3428691

Strand: Reverse

Name: ycdT [H]

Synonym: bglu_1g30200

Alternate gene names: 238028556

Gene position: 3428691-3427513 (Counterclockwise)

Preceding gene: 238028559

Following gene: 238028554

Centisome position: 87.77

GC content: 71.76

Gene sequence:

>1179_bases
ATGCACGTCGACCTTCTTACTTTTTATTTCCTCGCGATCGGAACGCTGCTTGCCAGCGCGGGCATGACGTTCTGGGAACA
TCGCACCCATCCCAATCGCAGCCGGGCGCTGCGCACGTTCGCGGCGGGCTATGCGACGCTCGCGATCGGCTGCGCCGTGG
TGCTGGTGCGGGGCCGCCTGCCCGGCGCGTGGGGGTCGGCGCTCGCCAACCTGGTGATGGTGTCCGGCTACCTGCTGATC
CTGGACGGCGTGGCCATCCTGAACGGCAGGTGTCGGCGCGGCGCTTCGCTGGGCCTGGTCGCGCTGCTGGCGCTGGCCTG
GGTCGTGGCCGGCACGCGCGGGCAGCAGACCATGTGGCTCTATCTGAGTGCGATTCCGATCGCGCTGGCGAGTGCCATGA
CCACGCGCGAACTGCTGCGCAACGCGGCGTTGCGCGTGCTCCCGGCCTGGCGCATCGCGGTTGCGGTGACGGGCCTGCAT
GCGCTGCTCTATCTGGCCCGCGCGCTCGCGCTGCCCTGGCTCGCGAGGGAATTCGGGCCGTCGTTCGTGCTGGCCGCCAG
CCAGCTCACGATGTACGAGGGCGTGCTCTATTCGGTGGTGCTGCCGATGACGCTGCTGCGGCTGGTGCGCGAGGAGACGC
ATGGCGAGCTGCTGCGCGAATCGCGTACCGACTACCTGACGCGGCTCGGCAGCCGCCGCTGGTTCTTCGAGGAGGGCGAG
CGCGTGGTGGCCGCGGCGGCGGGGCGGCAGGCGCTCGCCGTGCTGGCCTTCGACCTGGATCACTTCAAGCGCATCAACGA
CCGTCACGGCCACAAGACCGGCGACGAGGTGCTGAAGACTTTCGCGGAAGTCGTGCGCGGCGAGCTGGGCGAGGGCGCCA
TCGTCGCGCGCCTCGGCGGCGAGGAGTTCGCCGCGCTGCTGGCCGGTGCCGATGCGCAGCGCGCGGCGGCACTGGGCAGG
GCCGTCGCGCGCCGCTTCGCCGAGACGATCGACAGCCGCGGCGACGGCCTCGAGATCCGGGCCACCGTCAGCATCGGTCT
GGCCGCCGACGAGGCCGGCACCCCGCCCCTCCCGCCGCCCCTCCCGCCGGCGCTGCTGCTGGCCGACCTGCTGGCCAGTG
CCGACGGCGCGCTGTACCGCGCGAAATCGCTGGGCGGCAACCGGCTCGAACGGGCATAG

Upstream 100 bases:

>100_bases
CGGCACGCACCGAAATTTGACCATCACGGCGGGTGTCGAAGCGCCAGCGTAGAATGCCGCTGCTGCCGTGCGGTGCCGGG
CATCAGTTCTTTCGTCAGCG

Downstream 100 bases:

>100_bases
CGCCGCCTCGCCCGCGCGCCGCGCGCCGCGCGAGGCCGGCTGTCGGCAGGACGAGCCGGCCTCGGCCGGCCGGTGCGATG
GCGAACGCCCCGCATGCCGT

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MHVDLLTFYFLAIGTLLASAGMTFWEHRTHPNRSRALRTFAAGYATLAIGCAVVLVRGRLPGAWGSALANLVMVSGYLLI
LDGVAILNGRCRRGASLGLVALLALAWVVAGTRGQQTMWLYLSAIPIALASAMTTRELLRNAALRVLPAWRIAVAVTGLH
ALLYLARALALPWLAREFGPSFVLAASQLTMYEGVLYSVVLPMTLLRLVREETHGELLRESRTDYLTRLGSRRWFFEEGE
RVVAAAAGRQALAVLAFDLDHFKRINDRHGHKTGDEVLKTFAEVVRGELGEGAIVARLGGEEFAALLAGADAQRAAALGR
AVARRFAETIDSRGDGLEIRATVSIGLAADEAGTPPLPPPLPPALLLADLLASADGALYRAKSLGGNRLERA

Sequences:

>Translated_392_residues
MHVDLLTFYFLAIGTLLASAGMTFWEHRTHPNRSRALRTFAAGYATLAIGCAVVLVRGRLPGAWGSALANLVMVSGYLLI
LDGVAILNGRCRRGASLGLVALLALAWVVAGTRGQQTMWLYLSAIPIALASAMTTRELLRNAALRVLPAWRIAVAVTGLH
ALLYLARALALPWLAREFGPSFVLAASQLTMYEGVLYSVVLPMTLLRLVREETHGELLRESRTDYLTRLGSRRWFFEEGE
RVVAAAAGRQALAVLAFDLDHFKRINDRHGHKTGDEVLKTFAEVVRGELGEGAIVARLGGEEFAALLAGADAQRAAALGR
AVARRFAETIDSRGDGLEIRATVSIGLAADEAGTPPLPPPLPPALLLADLLASADGALYRAKSLGGNRLERA
>Mature_392_residues
MHVDLLTFYFLAIGTLLASAGMTFWEHRTHPNRSRALRTFAAGYATLAIGCAVVLVRGRLPGAWGSALANLVMVSGYLLI
LDGVAILNGRCRRGASLGLVALLALAWVVAGTRGQQTMWLYLSAIPIALASAMTTRELLRNAALRVLPAWRIAVAVTGLH
ALLYLARALALPWLAREFGPSFVLAASQLTMYEGVLYSVVLPMTLLRLVREETHGELLRESRTDYLTRLGSRRWFFEEGE
RVVAAAAGRQALAVLAFDLDHFKRINDRHGHKTGDEVLKTFAEVVRGELGEGAIVARLGGEEFAALLAGADAQRAAALGR
AVARRFAETIDSRGDGLEIRATVSIGLAADEAGTPPLPPPLPPALLLADLLASADGALYRAKSLGGNRLERA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=171, Percent_Identity=35.0877192982456, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787802, Length=169, Percent_Identity=32.5443786982249, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788085, Length=172, Percent_Identity=29.0697674418605, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI87081881, Length=193, Percent_Identity=34.7150259067358, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI145693134, Length=189, Percent_Identity=33.8624338624339, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786584, Length=143, Percent_Identity=32.1678321678322, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081977, Length=116, Percent_Identity=35.3448275862069, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87081974, Length=137, Percent_Identity=28.4671532846715, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42088; Mature: 42088

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVDLLTFYFLAIGTLLASAGMTFWEHRTHPNRSRALRTFAAGYATLAIGCAVVLVRGRL
CCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PGAWGSALANLVMVSGYLLILDGVAILNGRCRRGASLGLVALLALAWVVAGTRGQQTMWL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHH
YLSAIPIALASAMTTRELLRNAALRVLPAWRIAVAVTGLHALLYLARALALPWLAREFGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SFVLAASQLTMYEGVLYSVVLPMTLLRLVREETHGELLRESRTDYLTRLGSRRWFFEEGE
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCC
RVVAAAAGRQALAVLAFDLDHFKRINDRHGHKTGDEVLKTFAEVVRGELGEGAIVARLGG
EEEEHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECH
EEFAALLAGADAQRAAALGRAVARRFAETIDSRGDGLEIRATVSIGLAADEAGTPPLPPP
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCCCCCCC
LPPALLLADLLASADGALYRAKSLGGNRLERA
CCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MHVDLLTFYFLAIGTLLASAGMTFWEHRTHPNRSRALRTFAAGYATLAIGCAVVLVRGRL
CCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PGAWGSALANLVMVSGYLLILDGVAILNGRCRRGASLGLVALLALAWVVAGTRGQQTMWL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHH
YLSAIPIALASAMTTRELLRNAALRVLPAWRIAVAVTGLHALLYLARALALPWLAREFGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SFVLAASQLTMYEGVLYSVVLPMTLLRLVREETHGELLRESRTDYLTRLGSRRWFFEEGE
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCC
RVVAAAAGRQALAVLAFDLDHFKRINDRHGHKTGDEVLKTFAEVVRGELGEGAIVARLGG
EEEEHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECH
EEFAALLAGADAQRAAALGRAVARRFAETIDSRGDGLEIRATVSIGLAADEAGTPPLPPP
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCCCCCCCC
LPPALLLADLLASADGALYRAKSLGGNRLERA
CCHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]