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The map label for this gene is proP [H]

Identifier: 238028536

GI number: 238028536

Start: 3396884

End: 3398542

Strand: Direct

Name: proP [H]

Synonym: bglu_1g30000

Alternate gene names: 238028536

Gene position: 3396884-3398542 (Clockwise)

Preceding gene: 238028535

Following gene: 238028538

Centisome position: 86.95

GC content: 65.76

Gene sequence:

>1659_bases
ATGGCGACAGTAGGCGAACACATCGCGCATTCGCCGATGACGAGTGACGAGAAGCGGGTGATCTTCGCGTCGTCGCTCGG
CACCGTGTTCGAGTGGTACGACTTTTATCTGGCCGGCTCGCTCGCGGCCTACATCAGCAAGAGCTTCTTTTCCGGCGTCA
ACCCGACCGCCGCGTTCATCTTCACGCTGCTCGGGTTCGCTGCCGGCTTCGCCGTGCGCCCGTTCGGCGCGATCGTGTTC
GGCCGGCTCGGCGACATGGTCGGGCGCAAGTACACGTTCCTGATGACCATCGTGATCATGGGGCTGTCGACCTTCGTGGT
GGGCTTCCTGCCCGGCTACGCGTCGATCGGCATTGCCGCGCCGGTGATCTTCATCGTGATGCGGCTGCTGCAGGGGCTCG
CGCTGGGCGGCGAGTACGGCGGCGCGGCCACCTACGTGGCCGAGCACGCGCCGTCGAACCGGCGCGGCTTCTATACCGCG
TGGATCCAGACCACCGCCACGCTGGGCCTGTTCCTGTCGCTGCTGGTGATTCTCGGCGTGCGCCTGGCGCTCGGCGAGGA
CGCGTTCGCCGCCTGGGCATGGCGCATCCCGTTCGTCGCCTCGATCCTGCTGCTCGGCGTGTCGGTATGGATTCGGCTGC
AACTCGACGAATCGCCGGTGTTCCTGCGCATCAAGGCCGAGGGCAAGACCTCGAAGGCGCCGCTGACCGAGGCGTTCGGC
CAGTGGAAGAACCTGAAGATCGTGATCCTCGCGCTGGTCGGGCTGACCGCCGGGCAGGCCGTGGTCTGGTACACGGGCCA
GTTCTACGCGCTGTTCTTCCTGACCCAGACGCTGAAGGTGGACGGCAGCAGCGCCAACATCCTGATCGCCATCGCGCTCC
TGATCGGCACACCGTTCTTCCTGTTCTTCGGCTCGCTGTCGGACCGCATCGGCCGCAAGCCGATCATCCTGGCCGGCTGC
CTGATCGCCGCGCTCTCCTACTTCCCGCTGTTCAAGGCGCTCACGCACTACACGAACCCGGCGCTGGAGACCGCAACGCA
GAAGGCGCCGATCAGCGTGATCGCGAACCCGGACGAATGCTCGTTCCAGTTCAACCCGGTGGGCACGGCGAAGTTCACGA
ACTCGTGCGACGTGGCGAAGGCGGCGCTGGCGAAGGCCGGGCTCAACTACGACAACGTGGCCGCGCCGGCCGGCACGCTG
GCGCAGATCAAGGTGGGCGAGACGGTGATCGACACCTATGACGGCAAGGCCGCCGACGCCAAGCTGAAGGCCGCCGCGTT
CGACAAGACGCTGGCCGCGACGCTGAAGGGCGCCGGCTACCCGGCGAAGGCCGACCCGTCGCAGATCAACTGGCCGATGG
CGATCGTGGTGCTGACGATCCTCGTGATCTTCGTGACGATGGTGTACGGGCCGATCGCGGCGATGCTGGTGGAGATGTTC
CCGACGCGGATCCGCTACACGTCGATGTCGCTGCCCTATCACATCGGCAACGGCTGGTTCGGCGGCTTCCTGCCGGCCAC
GGCGTTCGCGATCGTGGCCGCCAAGGGCAACATCTACTCGGGGCTCTGGTATCCGATCACGATCGCGCTGGTGACGTTCG
TGATCGGGCTGCTGTTCGTGAAGGAGACGAAGGGGTCGAACATCTACTCGCAGGATTGA

Upstream 100 bases:

>100_bases
GCCGCACCAGTCAGTAGGCCGTAAGTTTGCCCGCCAATAATCGTCGCCAGTTCGTCAGGATGGCGAACCGACATATCGAC
ATCAAGATTGGAGACGACTC

Downstream 100 bases:

>100_bases
GGCGAGCGGGCGGGGCGGTGGCGCCTAGCTGAAAAAATTCGAGGAAAGTGTTGACACTGCCACGATCTTTCCTCATAATC
CGTCTTCTGTTGGCGAATTA

Product: major facilitator family transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MATVGEHIAHSPMTSDEKRVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGAIVF
GRLGDMVGRKYTFLMTIVIMGLSTFVVGFLPGYASIGIAAPVIFIVMRLLQGLALGGEYGGAATYVAEHAPSNRRGFYTA
WIQTTATLGLFLSLLVILGVRLALGEDAFAAWAWRIPFVASILLLGVSVWIRLQLDESPVFLRIKAEGKTSKAPLTEAFG
QWKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGSSANILIAIALLIGTPFFLFFGSLSDRIGRKPIILAGC
LIAALSYFPLFKALTHYTNPALETATQKAPISVIANPDECSFQFNPVGTAKFTNSCDVAKAALAKAGLNYDNVAAPAGTL
AQIKVGETVIDTYDGKAADAKLKAAAFDKTLAATLKGAGYPAKADPSQINWPMAIVVLTILVIFVTMVYGPIAAMLVEMF
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPITIALVTFVIGLLFVKETKGSNIYSQD

Sequences:

>Translated_552_residues
MATVGEHIAHSPMTSDEKRVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGAIVF
GRLGDMVGRKYTFLMTIVIMGLSTFVVGFLPGYASIGIAAPVIFIVMRLLQGLALGGEYGGAATYVAEHAPSNRRGFYTA
WIQTTATLGLFLSLLVILGVRLALGEDAFAAWAWRIPFVASILLLGVSVWIRLQLDESPVFLRIKAEGKTSKAPLTEAFG
QWKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGSSANILIAIALLIGTPFFLFFGSLSDRIGRKPIILAGC
LIAALSYFPLFKALTHYTNPALETATQKAPISVIANPDECSFQFNPVGTAKFTNSCDVAKAALAKAGLNYDNVAAPAGTL
AQIKVGETVIDTYDGKAADAKLKAAAFDKTLAATLKGAGYPAKADPSQINWPMAIVVLTILVIFVTMVYGPIAAMLVEMF
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPITIALVTFVIGLLFVKETKGSNIYSQD
>Mature_551_residues
ATVGEHIAHSPMTSDEKRVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGAIVFG
RLGDMVGRKYTFLMTIVIMGLSTFVVGFLPGYASIGIAAPVIFIVMRLLQGLALGGEYGGAATYVAEHAPSNRRGFYTAW
IQTTATLGLFLSLLVILGVRLALGEDAFAAWAWRIPFVASILLLGVSVWIRLQLDESPVFLRIKAEGKTSKAPLTEAFGQ
WKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGSSANILIAIALLIGTPFFLFFGSLSDRIGRKPIILAGCL
IAALSYFPLFKALTHYTNPALETATQKAPISVIANPDECSFQFNPVGTAKFTNSCDVAKAALAKAGLNYDNVAAPAGTLA
QIKVGETVIDTYDGKAADAKLKAAAFDKTLAATLKGAGYPAKADPSQINWPMAIVVLTILVIFVTMVYGPIAAMLVEMFP
TRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPITIALVTFVIGLLFVKETKGSNIYSQD

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=315, Percent_Identity=34.2857142857143, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1788292, Length=328, Percent_Identity=35.0609756097561, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1788942, Length=339, Percent_Identity=35.3982300884956, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1789941, Length=220, Percent_Identity=41.3636363636364, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI87082231, Length=236, Percent_Identity=26.271186440678, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87082404, Length=325, Percent_Identity=24.3076923076923, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 59196; Mature: 59065

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVGEHIAHSPMTSDEKRVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFI
CCCCHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FTLLGFAAGFAVRPFGAIVFGRLGDMVGRKYTFLMTIVIMGLSTFVVGFLPGYASIGIAA
HHHHHHHHCCHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
PVIFIVMRLLQGLALGGEYGGAATYVAEHAPSNRRGFYTAWIQTTATLGLFLSLLVILGV
HHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
RLALGEDAFAAWAWRIPFVASILLLGVSVWIRLQLDESPVFLRIKAEGKTSKAPLTEAFG
HHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEECCCCCCCCHHHHHC
QWKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGSSANILIAIALLIGTPFF
CCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCHHH
LFFGSLSDRIGRKPIILAGCLIAALSYFPLFKALTHYTNPALETATQKAPISVIANPDEC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEECCCCC
SFQFNPVGTAKFTNSCDVAKAALAKAGLNYDNVAAPAGTLAQIKVGETVIDTYDGKAADA
CEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCEEEECCCCCCCCC
KLKAAAFDKTLAATLKGAGYPAKADPSQINWPMAIVVLTILVIFVTMVYGPIAAMLVEMF
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPITIALVTFVIGLLFV
HHHEEEEEECCCEEECCCCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHH
KETKGSNIYSQD
CCCCCCCCCCCC
>Mature Secondary Structure 
ATVGEHIAHSPMTSDEKRVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFI
CCCHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FTLLGFAAGFAVRPFGAIVFGRLGDMVGRKYTFLMTIVIMGLSTFVVGFLPGYASIGIAA
HHHHHHHHCCHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
PVIFIVMRLLQGLALGGEYGGAATYVAEHAPSNRRGFYTAWIQTTATLGLFLSLLVILGV
HHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
RLALGEDAFAAWAWRIPFVASILLLGVSVWIRLQLDESPVFLRIKAEGKTSKAPLTEAFG
HHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEECCCCCCCCHHHHHC
QWKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGSSANILIAIALLIGTPFF
CCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCHHH
LFFGSLSDRIGRKPIILAGCLIAALSYFPLFKALTHYTNPALETATQKAPISVIANPDEC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCEEEEECCCCC
SFQFNPVGTAKFTNSCDVAKAALAKAGLNYDNVAAPAGTLAQIKVGETVIDTYDGKAADA
CEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCEEEECCCCCCCCC
KLKAAAFDKTLAATLKGAGYPAKADPSQINWPMAIVVLTILVIFVTMVYGPIAAMLVEMF
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPITIALVTFVIGLLFV
HHHEEEEEECCCEEECCCCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHH
KETKGSNIYSQD
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]