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The map label for this gene is dacB [H]

Identifier: 238028505

GI number: 238028505

Start: 3362222

End: 3363673

Strand: Direct

Name: dacB [H]

Synonym: bglu_1g29690

Alternate gene names: 238028505

Gene position: 3362222-3363673 (Clockwise)

Preceding gene: 238028499

Following gene: 238028506

Centisome position: 86.07

GC content: 70.66

Gene sequence:

>1452_bases
ATGAATCCCATTCGCCGCCTTACCGTTGTCCTTCATTCCCGCGCGCGTCACGGGCGCACCGCCCTCGTGCTGGCCGCCGC
GCGCGGCATCCTGCCGGCCTCGGTGCTGGCCGGGCTGCAACGCGCCCACGTGCCGGCCGCGAACCTCAGCGTGGTGGTGG
AGAAGATCGGCGCGCGCGCGCCGGTGGTGGACTGGAACGGCGAGCGGCCGATGCTGCCGGCCTCGACGATGAAGCTCGTG
ACCACCTACTCGGGGCTCGCGCTGCTCGGCGCCGACTACCGCTACCGCACCAGCGCCTGGGCCGACGGCCCGGTCGACCC
CACCGGCACGCTGCACGGCAACCTCTACATCAAGGGCACCGGCGATCCGAAGCTGGTGCCCGAGGAACTGATCGACCTGG
TCGACAAGATCCGGCGTGCCGGCATCGTCAACATCGACGGCGCGCTGGTGCTCGACAAGACCTTCTTCGCGCCGTCCACG
CGCGACCTGCCCGCGTTCGACGACGGCACCTTCGCGCCGTACAACGTCGGCCCCGACCCGCTGCTCTATGCCTTCAAGTC
GCTGTCGTTTACGGTCACCCCGGGCGACGGCGGCGCGGTGGCGATCGACGTGCAGCCGCCGCTCGCGAACCTGACGATCG
ACAACGAGGTCAGCGAGGGCGGCAGCTCCTGCGCGGCGGCGGCCGCGGCCGCGCGCCCGCATCTGACGACCGACCCCAAC
GGCGCGCTGACGGCCGCCTTCGCCGGGCGCTTCCCCCGGCACTGCGAACCGATCACCACCAACATCGCCGTGCTCGATCA
CTCGCAGTTCTTCTCGCAGGGCTTTCTCGCGCTGTGGCGCTCGACGGGCGGCATCTTCAACGGCCGCATCGTCGAGGGCA
AGGTGCCGACCACGGCGCGCCCGGTGGCCGTCCACCGCGGCCCGGTGCTGTCGAGCATCGTCTACGACATCAACAAGAAC
AGCAACAACACCATGGCGCGCAACCTGTTCCTGTCGCTCGGCGCGATCGAGGGCAAGCCGCCCGCCACGCCCGAGCAGTC
GGCCGGCGTGATCCGCGCGTTCCTCGCCAGAAGCGGCATCGCCATGCCGGAGCTGGTGCTGGAGAACGGCTCGGGCCTGT
CGCGCGAGGAACAGGTGAGCGCGCTGTCGCTCGCCGCGCTGCTGCAGGCGGCCAATGCGAGCCCGGTGGCCCAGCCGTTC
ATCGACTCGCTGCCGATCGTCGGCGTGGACGGCACCATGAAGCGGCGCCTGACCAACCAGCCCGTGCTCGGCAACGCGCA
CATCAAGACCGGCACGCTGCGCGACGTGCGTGCGATCGCCGGCTACGTGGCCTCGGCCGACGGCAACAGCTACGTGGTGG
TCAGCTTCATCAACGATGCCCATGCGGCCGCCGCGCGCGCGGCCCACGACGCGCTGCTCGAATGGGTCTACGCGGGGCCG
CACGGCAATTGA

Upstream 100 bases:

>100_bases
GCGCCGCGCGCGGCGCATTTCGTCATCCCGGCCGCGACGCTTGTCTCTACAATGTGCGCTCGCGGCCAAATCCGGCCGGC
TTCGCTCTACTCGCTCGTCG

Downstream 100 bases:

>100_bases
GCGGCCCCGGCGGCGCCCCCTCGGGCGCCGCGCCTTGCGGGCAAACCCTGCCCCGAACACTCCCTCTACGGAACTTGTCG
CGCCGCCGGCGGCGATTGCG

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MNPIRRLTVVLHSRARHGRTALVLAAARGILPASVLAGLQRAHVPAANLSVVVEKIGARAPVVDWNGERPMLPASTMKLV
TTYSGLALLGADYRYRTSAWADGPVDPTGTLHGNLYIKGTGDPKLVPEELIDLVDKIRRAGIVNIDGALVLDKTFFAPST
RDLPAFDDGTFAPYNVGPDPLLYAFKSLSFTVTPGDGGAVAIDVQPPLANLTIDNEVSEGGSSCAAAAAAARPHLTTDPN
GALTAAFAGRFPRHCEPITTNIAVLDHSQFFSQGFLALWRSTGGIFNGRIVEGKVPTTARPVAVHRGPVLSSIVYDINKN
SNNTMARNLFLSLGAIEGKPPATPEQSAGVIRAFLARSGIAMPELVLENGSGLSREEQVSALSLAALLQAANASPVAQPF
IDSLPIVGVDGTMKRRLTNQPVLGNAHIKTGTLRDVRAIAGYVASADGNSYVVVSFINDAHAAAARAAHDALLEWVYAGP
HGN

Sequences:

>Translated_483_residues
MNPIRRLTVVLHSRARHGRTALVLAAARGILPASVLAGLQRAHVPAANLSVVVEKIGARAPVVDWNGERPMLPASTMKLV
TTYSGLALLGADYRYRTSAWADGPVDPTGTLHGNLYIKGTGDPKLVPEELIDLVDKIRRAGIVNIDGALVLDKTFFAPST
RDLPAFDDGTFAPYNVGPDPLLYAFKSLSFTVTPGDGGAVAIDVQPPLANLTIDNEVSEGGSSCAAAAAAARPHLTTDPN
GALTAAFAGRFPRHCEPITTNIAVLDHSQFFSQGFLALWRSTGGIFNGRIVEGKVPTTARPVAVHRGPVLSSIVYDINKN
SNNTMARNLFLSLGAIEGKPPATPEQSAGVIRAFLARSGIAMPELVLENGSGLSREEQVSALSLAALLQAANASPVAQPF
IDSLPIVGVDGTMKRRLTNQPVLGNAHIKTGTLRDVRAIAGYVASADGNSYVVVSFINDAHAAAARAAHDALLEWVYAGP
HGN
>Mature_483_residues
MNPIRRLTVVLHSRARHGRTALVLAAARGILPASVLAGLQRAHVPAANLSVVVEKIGARAPVVDWNGERPMLPASTMKLV
TTYSGLALLGADYRYRTSAWADGPVDPTGTLHGNLYIKGTGDPKLVPEELIDLVDKIRRAGIVNIDGALVLDKTFFAPST
RDLPAFDDGTFAPYNVGPDPLLYAFKSLSFTVTPGDGGAVAIDVQPPLANLTIDNEVSEGGSSCAAAAAAARPHLTTDPN
GALTAAFAGRFPRHCEPITTNIAVLDHSQFFSQGFLALWRSTGGIFNGRIVEGKVPTTARPVAVHRGPVLSSIVYDINKN
SNNTMARNLFLSLGAIEGKPPATPEQSAGVIRAFLARSGIAMPELVLENGSGLSREEQVSALSLAALLQAANASPVAQPF
IDSLPIVGVDGTMKRRLTNQPVLGNAHIKTGTLRDVRAIAGYVASADGNSYVVVSFINDAHAAAARAAHDALLEWVYAGP
HGN

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=427, Percent_Identity=25.7611241217799, Blast_Score=135, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 50733; Mature: 50733

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIRRLTVVLHSRARHGRTALVLAAARGILPASVLAGLQRAHVPAANLSVVVEKIGARA
CCHHHHHHHHHHHHCCCCCEEEEEEHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
PVVDWNGERPMLPASTMKLVTTYSGLALLGADYRYRTSAWADGPVDPTGTLHGNLYIKGT
CEEECCCCCCCCCHHHHHHHHHHCCEEEECCCCEEECCCCCCCCCCCCCEEEEEEEEEEC
GDPKLVPEELIDLVDKIRRAGIVNIDGALVLDKTFFAPSTRDLPAFDDGTFAPYNVGPDP
CCCCCCHHHHHHHHHHHHHCCEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCH
LLYAFKSLSFTVTPGDGGAVAIDVQPPLANLTIDNEVSEGGSSCAAAAAAARPHLTTDPN
HHHHHHHCEEEEECCCCCEEEEECCCCCCCEEECCCCCCCCCHHHHHHHHCCCCCCCCCC
GALTAAFAGRFPRHCEPITTNIAVLDHSQFFSQGFLALWRSTGGIFNGRIVEGKVPTTAR
CCEEEHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCEECCEEEECCCCCCCC
PVAVHRGPVLSSIVYDINKNSNNTMARNLFLSLGAIEGKPPATPEQSAGVIRAFLARSGI
CEEEECCCHHHHHHHCCCCCCCCHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHCCC
AMPELVLENGSGLSREEQVSALSLAALLQAANASPVAQPFIDSLPIVGVDGTMKRRLTNQ
CCCHHEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEECCCCHHHHHHCCC
PVLGNAHIKTGTLRDVRAIAGYVASADGNSYVVVSFINDAHAAAARAAHDALLEWVYAGP
CCCCCCEEECCCHHHHHHHHHHHEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCC
HGN
CCC
>Mature Secondary Structure
MNPIRRLTVVLHSRARHGRTALVLAAARGILPASVLAGLQRAHVPAANLSVVVEKIGARA
CCHHHHHHHHHHHHCCCCCEEEEEEHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
PVVDWNGERPMLPASTMKLVTTYSGLALLGADYRYRTSAWADGPVDPTGTLHGNLYIKGT
CEEECCCCCCCCCHHHHHHHHHHCCEEEECCCCEEECCCCCCCCCCCCCEEEEEEEEEEC
GDPKLVPEELIDLVDKIRRAGIVNIDGALVLDKTFFAPSTRDLPAFDDGTFAPYNVGPDP
CCCCCCHHHHHHHHHHHHHCCEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCH
LLYAFKSLSFTVTPGDGGAVAIDVQPPLANLTIDNEVSEGGSSCAAAAAAARPHLTTDPN
HHHHHHHCEEEEECCCCCEEEEECCCCCCCEEECCCCCCCCCHHHHHHHHCCCCCCCCCC
GALTAAFAGRFPRHCEPITTNIAVLDHSQFFSQGFLALWRSTGGIFNGRIVEGKVPTTAR
CCEEEHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCEECCEEEECCCCCCCC
PVAVHRGPVLSSIVYDINKNSNNTMARNLFLSLGAIEGKPPATPEQSAGVIRAFLARSGI
CEEEECCCHHHHHHHCCCCCCCCHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHCCC
AMPELVLENGSGLSREEQVSALSLAALLQAANASPVAQPFIDSLPIVGVDGTMKRRLTNQ
CCCHHEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEECCCCHHHHHHCCC
PVLGNAHIKTGTLRDVRAIAGYVASADGNSYVVVSFINDAHAAAARAAHDALLEWVYAGP
CCCCCCEEECCCHHHHHHHHHHHEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCC
HGN
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]