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The map label for this gene is purN [H]

Identifier: 238028411

GI number: 238028411

Start: 3257454

End: 3258113

Strand: Reverse

Name: purN [H]

Synonym: bglu_1g28750

Alternate gene names: 238028411

Gene position: 3258113-3257454 (Counterclockwise)

Preceding gene: 238028417

Following gene: 238028410

Centisome position: 83.4

GC content: 70.15

Gene sequence:

>660_bases
ATGAAAAAACTTGTCATTCTGATTTCAGGTCGCGGCAGCAACATGGAGGCCATCGTCGACGCGTGCGAGCGCGAGAGGTG
GCCCGCCAGCGTGGCCGCCGTGATCGCAAACCGGCCCGACGCGGCCGGTTTGTCGTTTGCGGCCGCGCGCGGGATTCCCG
CCGTCGTGGTCGATCACCGCGATCACGACGGCCGCGAGGCGTTCGACGCCGCGCTGGCGGCCGAGATCGACCGCTTCGCC
CCGGATCTCGTCGTGCTCGCCGGCTTCATGCGGATCCTCACGCCGGCCTTCGTCACGCGTTACGAGGGCCGCATGCTGAA
CGTCCATCCGTCGCTGCTGCCGAGCTTCAAGGGCATGCGCACCCATGAGGCGGCGCTCGCCGCCGGCGTGGCGCTGCATG
GCGCGACCGTGCATTTCGTGATTCCCGAGCTCGACAGCGGCGCGATCGTCGCGCAGGCGGCGGTGCCGGTGCACGACGGC
GATACGGCTCAGACGCTGGCCGCGCGCGTGCTGGTGGCCGAGCATCAGCTCTATCCGCGCGCCGTGCGCTGGTTCGTCGA
GGGCCGGCTGCGGCTCGACGGCGGCCGTGCGGTGGTCGCGCCCGGCGCGTGCCGATGGCTCGTCGCCGCCGACAACAACG
AACAGGGTGAGGGTGTATGA

Upstream 100 bases:

>100_bases
TGGTGCGGGCGGATGCCGCAAAACGCAAGATTATAAACGTTCGGGCGGCGCGGCGGCTCGGGCGGCGGGAAACCGCCATC
CCGAATGGTAAAATCCGCGG

Downstream 100 bases:

>100_bases
AGCTGCATGGTTTTCTGATTGGTCAAACCGAGACCTTGCTCGCCGAGGTCCTGAAATTCACCGGCCCCGCCGACGCCGCC
ACCAGCCGGTTCTTCCGTGC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MKKLVILISGRGSNMEAIVDACERERWPASVAAVIANRPDAAGLSFAAARGIPAVVVDHRDHDGREAFDAALAAEIDRFA
PDLVVLAGFMRILTPAFVTRYEGRMLNVHPSLLPSFKGMRTHEAALAAGVALHGATVHFVIPELDSGAIVAQAAVPVHDG
DTAQTLAARVLVAEHQLYPRAVRWFVEGRLRLDGGRAVVAPGACRWLVAADNNEQGEGV

Sequences:

>Translated_219_residues
MKKLVILISGRGSNMEAIVDACERERWPASVAAVIANRPDAAGLSFAAARGIPAVVVDHRDHDGREAFDAALAAEIDRFA
PDLVVLAGFMRILTPAFVTRYEGRMLNVHPSLLPSFKGMRTHEAALAAGVALHGATVHFVIPELDSGAIVAQAAVPVHDG
DTAQTLAARVLVAEHQLYPRAVRWFVEGRLRLDGGRAVVAPGACRWLVAADNNEQGEGV
>Mature_219_residues
MKKLVILISGRGSNMEAIVDACERERWPASVAAVIANRPDAAGLSFAAARGIPAVVVDHRDHDGREAFDAALAAEIDRFA
PDLVVLAGFMRILTPAFVTRYEGRMLNVHPSLLPSFKGMRTHEAALAAGVALHGATVHFVIPELDSGAIVAQAAVPVHDG
DTAQTLAARVLVAEHQLYPRAVRWFVEGRLRLDGGRAVVAPGACRWLVAADNNEQGEGV

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=45.7894736842105, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=45.7894736842105, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=45.7894736842105, Blast_Score=183, Evalue=1e-46,
Organism=Escherichia coli, GI1788846, Length=190, Percent_Identity=50.5263157894737, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1787483, Length=198, Percent_Identity=31.3131313131313, Blast_Score=98, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17567511, Length=183, Percent_Identity=41.5300546448087, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320616, Length=199, Percent_Identity=29.6482412060301, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24582400, Length=189, Percent_Identity=42.8571428571429, Blast_Score=156, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23322; Mature: 23322

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVILISGRGSNMEAIVDACERERWPASVAAVIANRPDAAGLSFAAARGIPAVVVDHR
CCEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHCCCCEEEEECC
DHDGREAFDAALAAEIDRFAPDLVVLAGFMRILTPAFVTRYEGRMLNVHPSLLPSFKGMR
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCCC
THEAALAAGVALHGATVHFVIPELDSGAIVAQAAVPVHDGDTAQTLAARVLVAEHQLYPR
HHHHHHHHHHEECCCEEEEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
AVRWFVEGRLRLDGGRAVVAPGACRWLVAADNNEQGEGV
HHHHHHCCEEEECCCEEEECCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKKLVILISGRGSNMEAIVDACERERWPASVAAVIANRPDAAGLSFAAARGIPAVVVDHR
CCEEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHCCCCEEEEECC
DHDGREAFDAALAAEIDRFAPDLVVLAGFMRILTPAFVTRYEGRMLNVHPSLLPSFKGMR
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHCCCCCCCC
THEAALAAGVALHGATVHFVIPELDSGAIVAQAAVPVHDGDTAQTLAARVLVAEHQLYPR
HHHHHHHHHHEECCCEEEEEEECCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
AVRWFVEGRLRLDGGRAVVAPGACRWLVAADNNEQGEGV
HHHHHHCCEEEECCCEEEECCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]