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The map label for this gene is hindIIIM [H]

Identifier: 238027943

GI number: 238027943

Start: 2623700

End: 2624698

Strand: Reverse

Name: hindIIIM [H]

Synonym: bglu_1g23880

Alternate gene names: 238027943

Gene position: 2624698-2623700 (Counterclockwise)

Preceding gene: 238027944

Following gene: 238027932

Centisome position: 67.19

GC content: 63.36

Gene sequence:

>999_bases
ATGGACCACGCTCTCGCCTACGAACCTTCCCTCAACGAGACCAGCACCGCCTCGTTCGGTTCGCTCATGCCTCATGTTCC
GACGCGTGCGCGTTTGTCGCGTGTCTCCCGTCCGTCCCTGGATGTCGCCGTGCGGCTCGGCAACGCGCTCGACGAGATGC
GCAAGATCCCCGACGCGAGCGTGCACCTGATCGCCACCGATCCTCCGTATTTCATCGACGGGATGGGTTCCGACTGGAAC
AAGACGAGCCTGCGCACCCGGACCGACAAGGCCGGCGTGGTGGGCAGTCTGCCGGTCGGTATGAAGTTCGACCCGGCGCA
GGCGCGCGCTTTCCAGGAGTTCATGGAGCCGATCGCCAAGGAAGCCTTCCGCATTCTGAAGCCGGGCGGTTTCTTCATCG
CCTTCTCGCAAGCACGCCTCTACCATCGGCTCGCGGTCGCCGCAGAAGATGCAGGGTTCGAGATTCGAGACATGCTGGCG
TGGCACTACGAGGGCCAGGCCAAGGCGTTCTCCATGAACCACTTCATCGCCAAGATGAAGATCAGCGACGCCGACAAGCA
ACACCTGATCGATTCGATCGGCGGCCGTAAGACGCCTCAACTGAAGCCCCAGATGGAGCCGATGGTGATGGCGCAGAAGC
CCCGCGTCGGCACCTTCGTGGAGAACTGGAAGCAGTTCGAGACCGGCCTGGTGGACACCACCGCCTCGTTGGACGGGAAG
TTCCCCGGCAATGTCATGGACGTCGCCAAGCCGTCGCGCGACGAAAAGGGCGCGGGCAACGAACACTTGACCGTGAAGCC
GGTCCTCTTGATGGAGCACCTGATCCGTCTGTTCTCGATCCCCGGCCAGGTCGTGCTCGATCCGTTCCTCGGCAGCGGGA
CCACCGGCGTCGCCGCGATCAACACGGGCCGCGAGTTCATCGGCGTGGAGATCGACCCGGACTACGCTCGAATCGCCAGC
AAGCGCATCGCCGCAGCGAGGGAAAACCATGCTGGATAA

Upstream 100 bases:

>100_bases
GTCCTTGAAAATAGGGTGATTTTAGTCGTGCCCAAGGCATTGACTTAAGCCCAGAACCTGGTTTCCTGGAAGTAGACCCA
TCTACATCCGAGGACCGGAC

Downstream 100 bases:

>100_bases
GGCTTACACCGAAGCCGATGTCCAGCATTACTACGGTCTGTTGGTTGTCGAGCACCAGAAGCACCTCGCCACGCACGGCG
TCAAACTCCCGCCGCTCCTC

Product: putative DNA methylase

Products: NA

Alternate protein names: M.HindIII; Adenine-specific methyltransferase HindIII [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MDHALAYEPSLNETSTASFGSLMPHVPTRARLSRVSRPSLDVAVRLGNALDEMRKIPDASVHLIATDPPYFIDGMGSDWN
KTSLRTRTDKAGVVGSLPVGMKFDPAQARAFQEFMEPIAKEAFRILKPGGFFIAFSQARLYHRLAVAAEDAGFEIRDMLA
WHYEGQAKAFSMNHFIAKMKISDADKQHLIDSIGGRKTPQLKPQMEPMVMAQKPRVGTFVENWKQFETGLVDTTASLDGK
FPGNVMDVAKPSRDEKGAGNEHLTVKPVLLMEHLIRLFSIPGQVVLDPFLGSGTTGVAAINTGREFIGVEIDPDYARIAS
KRIAAARENHAG

Sequences:

>Translated_332_residues
MDHALAYEPSLNETSTASFGSLMPHVPTRARLSRVSRPSLDVAVRLGNALDEMRKIPDASVHLIATDPPYFIDGMGSDWN
KTSLRTRTDKAGVVGSLPVGMKFDPAQARAFQEFMEPIAKEAFRILKPGGFFIAFSQARLYHRLAVAAEDAGFEIRDMLA
WHYEGQAKAFSMNHFIAKMKISDADKQHLIDSIGGRKTPQLKPQMEPMVMAQKPRVGTFVENWKQFETGLVDTTASLDGK
FPGNVMDVAKPSRDEKGAGNEHLTVKPVLLMEHLIRLFSIPGQVVLDPFLGSGTTGVAAINTGREFIGVEIDPDYARIAS
KRIAAARENHAG
>Mature_332_residues
MDHALAYEPSLNETSTASFGSLMPHVPTRARLSRVSRPSLDVAVRLGNALDEMRKIPDASVHLIATDPPYFIDGMGSDWN
KTSLRTRTDKAGVVGSLPVGMKFDPAQARAFQEFMEPIAKEAFRILKPGGFFIAFSQARLYHRLAVAAEDAGFEIRDMLA
WHYEGQAKAFSMNHFIAKMKISDADKQHLIDSIGGRKTPQLKPQMEPMVMAQKPRVGTFVENWKQFETGLVDTTASLDGK
FPGNVMDVAKPSRDEKGAGNEHLTVKPVLLMEHLIRLFSIPGQVVLDPFLGSGTTGVAAINTGREFIGVEIDPDYARIAS
KRIAAARENHAG

Specific function: This methylase recognizes the double-stranded sequence AAGCTT, causes specific methylation on A-1 on both strands, and protects the DNA from cleavage by the HindIII endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=296, Percent_Identity=27.027027027027, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 36474; Mature: 36474

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHALAYEPSLNETSTASFGSLMPHVPTRARLSRVSRPSLDVAVRLGNALDEMRKIPDAS
CCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCHHHEEHHHHHHHHHHHCCCCC
VHLIATDPPYFIDGMGSDWNKTSLRTRTDKAGVVGSLPVGMKFDPAQARAFQEFMEPIAK
EEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCEECCCCCCEECHHHHHHHHHHHHHHHH
EAFRILKPGGFFIAFSQARLYHRLAVAAEDAGFEIRDMLAWHYEGQAKAFSMNHFIAKMK
HHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEHHHEEEEEE
ISDADKQHLIDSIGGRKTPQLKPQMEPMVMAQKPRVGTFVENWKQFETGLVDTTASLDGK
ECCCHHHHHHHHHCCCCCCCCCCCCCCHHEECCCCCCHHHHHHHHHHCCCEECHHCCCCC
FPGNVMDVAKPSRDEKGAGNEHLTVKPVLLMEHLIRLFSIPGQVVLDPFLGSGTTGVAAI
CCCCCHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEE
NTGREFIGVEIDPDYARIASKRIAAARENHAG
ECCCEEEEEEECCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDHALAYEPSLNETSTASFGSLMPHVPTRARLSRVSRPSLDVAVRLGNALDEMRKIPDAS
CCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHCCCCCHHHEEHHHHHHHHHHHCCCCC
VHLIATDPPYFIDGMGSDWNKTSLRTRTDKAGVVGSLPVGMKFDPAQARAFQEFMEPIAK
EEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCEECCCCCCEECHHHHHHHHHHHHHHHH
EAFRILKPGGFFIAFSQARLYHRLAVAAEDAGFEIRDMLAWHYEGQAKAFSMNHFIAKMK
HHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEHHHEEEEEE
ISDADKQHLIDSIGGRKTPQLKPQMEPMVMAQKPRVGTFVENWKQFETGLVDTTASLDGK
ECCCHHHHHHHHHCCCCCCCCCCCCCCHHEECCCCCCHHHHHHHHHHCCCEECHHCCCCC
FPGNVMDVAKPSRDEKGAGNEHLTVKPVLLMEHLIRLFSIPGQVVLDPFLGSGTTGVAAI
CCCCCHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEE
NTGREFIGVEIDPDYARIASKRIAAARENHAG
ECCCEEEEEEECCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7959067; 7542800 [H]