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The map label for this gene is tilS [H]

Identifier: 238027917

GI number: 238027917

Start: 2592116

End: 2593555

Strand: Reverse

Name: tilS [H]

Synonym: bglu_1g23590

Alternate gene names: 238027917

Gene position: 2593555-2592116 (Counterclockwise)

Preceding gene: 238027918

Following gene: 238027916

Centisome position: 66.39

GC content: 74.31

Gene sequence:

>1440_bases
ATGATTTCTCCGCATGAGACTGCCGCGGACCGCGTCGTGCTCGACGCGGTCCGCGGCGCTTTGGACGCGCTGCCCGAGGC
CGCCACGATCGCGATCGCGTACAGCGGCGGGCTCGACTCGTCGGTGCTGCTCGACGCGGCCGTGCGCGTGGCCGGCGCCG
CGCGCTGCGTGGCGCTGCATGTCCACCACGGGCTGAGCCCCAACGCCGATGCCTGGCTCGCGCATGCCGCGGCGTGCGCG
GCCGCGCTTGGCGTGTGCTTCGATGCACAACGCGTCGAGGTGCCGCGCGAGAGCGGGCTCGGCGTCGAGGCGAGCGCGCG
CGAGCTTCGCTATCGCGCGCTCGACGCGCTGGGCGAGCGCCACGGCGCGCAGGCGCTGTGGATCGCGCAACACGCCGACG
ACCAGGCCGAAACGGTGCTGCTGCAATTGCTGCGCGGCGCGGGGCTGGCCGGCATCGCCGCGATGGCGCCGCGCTACCTG
CCCGACGGCGCGACGATCGTGCGCATGCGTCCGCTGCTGTCGCTGTTGCGCGCGCAGCTCGAACGCTACGCCGAGGCGCG
CGCGCTGCGCTGGATCGACGACGAGTCGAATGCCGACACGCGTTATGCGCGCAATGCGCTGCGTGCGCGAGTCCTGCCCG
AACTCGCCGTCCACTTTCCCGGATTCCGTGACGCGCTGGCGCGTACCGCCCAACATGCGGCCGCCGCGCAGCGGCTGCTC
GACGTGCTCGCGGCCGACGATCTCGCCGGCGCGACGCGCGACGACGGCCGCGTGCTCTCGCGCGAGCGCCTGCTCGGCTT
CGACGACGAACGCGGCGCGAACCTGCTGCGCCACTGGATGCGCACGCTCGGCCTGCCCGGCGCCTCGGCCGCACGGCTCG
CCGAGATGATCAAGCAGCTGCGCGCCGCGCAGGTCTCGCATGCCTTGCGTATCGATCACGCAGGGCAATGCCTGCGGCTC
TATCGCGACGACGTGTACTGGGAAGCTGGCGACAGCGCTGATCCGGCCGACGACGACAGCGCGCCCGAGCGTCCGCTCGC
GCGGCTCGAGTGGGCGGGGCAGGAGGTCTGGCATCTGCCGGCGTGGCGCGGCAGCTTCGTGTTCCGGCCGGTCGACGCGG
CGCCGGCCGCGGCAGCCGGCGCCAGCGATCTGGTCGCCGAGGCCGTGCTGGCGGGCGCGCCGCTCGTGGCGCGCGCACGG
GCCGGCGGCGAGCGGATGCGAATGTCGCCGGGCGGGCCGTCGCGCACCCTCAAGAATCTGTTCCAGGAGCGCGGCGTGCC
GGCGTGGCAGCGGGACGTTCCGCTGCTGCATGTCGGCGAGACGCTGCTGTTCGTGCCGCGGCTCGGCGTCAATCGCGCGC
CGGAGGCGGGCGGGGCGGGCGCCGGCGTGGCGGGTCCGTGCCGGCGCATCGAGTGGCGGCCCGATCTGCTGATTGCCTGA

Upstream 100 bases:

>100_bases
CGCATGGAGCGTCTGCTCGCCTACGGCAAGTTCAAGGAAACGACGCCGGGCGTCTGAGCGCCGTGGTCGCTTTTGCGAGC
CGCGCGCAAGCGTGCCGCCG

Downstream 100 bases:

>100_bases
CCATCGGTTTATAAAAAGGGTTCGAGCAATTCGCAAAACGACGGCCGAGTGCCGATTCGCGTAATAAAAAGCGACGGACC
AGTTTCTGACAACTTGTCAA

Product: tRNA-Ile-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MISPHETAADRVVLDAVRGALDALPEAATIAIAYSGGLDSSVLLDAAVRVAGAARCVALHVHHGLSPNADAWLAHAAACA
AALGVCFDAQRVEVPRESGLGVEASARELRYRALDALGERHGAQALWIAQHADDQAETVLLQLLRGAGLAGIAAMAPRYL
PDGATIVRMRPLLSLLRAQLERYAEARALRWIDDESNADTRYARNALRARVLPELAVHFPGFRDALARTAQHAAAAQRLL
DVLAADDLAGATRDDGRVLSRERLLGFDDERGANLLRHWMRTLGLPGASAARLAEMIKQLRAAQVSHALRIDHAGQCLRL
YRDDVYWEAGDSADPADDDSAPERPLARLEWAGQEVWHLPAWRGSFVFRPVDAAPAAAAGASDLVAEAVLAGAPLVARAR
AGGERMRMSPGGPSRTLKNLFQERGVPAWQRDVPLLHVGETLLFVPRLGVNRAPEAGGAGAGVAGPCRRIEWRPDLLIA

Sequences:

>Translated_479_residues
MISPHETAADRVVLDAVRGALDALPEAATIAIAYSGGLDSSVLLDAAVRVAGAARCVALHVHHGLSPNADAWLAHAAACA
AALGVCFDAQRVEVPRESGLGVEASARELRYRALDALGERHGAQALWIAQHADDQAETVLLQLLRGAGLAGIAAMAPRYL
PDGATIVRMRPLLSLLRAQLERYAEARALRWIDDESNADTRYARNALRARVLPELAVHFPGFRDALARTAQHAAAAQRLL
DVLAADDLAGATRDDGRVLSRERLLGFDDERGANLLRHWMRTLGLPGASAARLAEMIKQLRAAQVSHALRIDHAGQCLRL
YRDDVYWEAGDSADPADDDSAPERPLARLEWAGQEVWHLPAWRGSFVFRPVDAAPAAAAGASDLVAEAVLAGAPLVARAR
AGGERMRMSPGGPSRTLKNLFQERGVPAWQRDVPLLHVGETLLFVPRLGVNRAPEAGGAGAGVAGPCRRIEWRPDLLIA
>Mature_479_residues
MISPHETAADRVVLDAVRGALDALPEAATIAIAYSGGLDSSVLLDAAVRVAGAARCVALHVHHGLSPNADAWLAHAAACA
AALGVCFDAQRVEVPRESGLGVEASARELRYRALDALGERHGAQALWIAQHADDQAETVLLQLLRGAGLAGIAAMAPRYL
PDGATIVRMRPLLSLLRAQLERYAEARALRWIDDESNADTRYARNALRARVLPELAVHFPGFRDALARTAQHAAAAQRLL
DVLAADDLAGATRDDGRVLSRERLLGFDDERGANLLRHWMRTLGLPGASAARLAEMIKQLRAAQVSHALRIDHAGQCLRL
YRDDVYWEAGDSADPADDDSAPERPLARLEWAGQEVWHLPAWRGSFVFRPVDAAPAAAAGASDLVAEAVLAGAPLVARAR
AGGERMRMSPGGPSRTLKNLFQERGVPAWQRDVPLLHVGETLLFVPRLGVNRAPEAGGAGAGVAGPCRRIEWRPDLLIA

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=439, Percent_Identity=37.8132118451025, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 51267; Mature: 51267

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00539 PYROKININ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISPHETAADRVVLDAVRGALDALPEAATIAIAYSGGLDSSVLLDAAVRVAGAARCVALH
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEE
VHHGLSPNADAWLAHAAACAAALGVCFDAQRVEVPRESGLGVEASARELRYRALDALGER
ECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHH
HGAQALWIAQHADDQAETVLLQLLRGAGLAGIAAMAPRYLPDGATIVRMRPLLSLLRAQL
CCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
ERYAEARALRWIDDESNADTRYARNALRARVLPELAVHFPGFRDALARTAQHAAAAQRLL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DVLAADDLAGATRDDGRVLSRERLLGFDDERGANLLRHWMRTLGLPGASAARLAEMIKQL
HHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RAAQVSHALRIDHAGQCLRLYRDDVYWEAGDSADPADDDSAPERPLARLEWAGQEVWHLP
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHEECC
AWRGSFVFRPVDAAPAAAAGASDLVAEAVLAGAPLVARARAGGERMRMSPGGPSRTLKNL
CCCCCEEEECCCCCCCHHCCHHHHHHHHHHHCCCHHHHHHCCCCEEECCCCCHHHHHHHH
FQERGVPAWQRDVPLLHVGETLLFVPRLGVNRAPEAGGAGAGVAGPCRRIEWRPDLLIA
HHHCCCCCHHHCCCEEEECCHHEECCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MISPHETAADRVVLDAVRGALDALPEAATIAIAYSGGLDSSVLLDAAVRVAGAARCVALH
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEE
VHHGLSPNADAWLAHAAACAAALGVCFDAQRVEVPRESGLGVEASARELRYRALDALGER
ECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHH
HGAQALWIAQHADDQAETVLLQLLRGAGLAGIAAMAPRYLPDGATIVRMRPLLSLLRAQL
CCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
ERYAEARALRWIDDESNADTRYARNALRARVLPELAVHFPGFRDALARTAQHAAAAQRLL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DVLAADDLAGATRDDGRVLSRERLLGFDDERGANLLRHWMRTLGLPGASAARLAEMIKQL
HHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RAAQVSHALRIDHAGQCLRLYRDDVYWEAGDSADPADDDSAPERPLARLEWAGQEVWHLP
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHEECC
AWRGSFVFRPVDAAPAAAAGASDLVAEAVLAGAPLVARARAGGERMRMSPGGPSRTLKNL
CCCCCEEEECCCCCCCHHCCHHHHHHHHHHHCCCHHHHHHCCCCEEECCCCCHHHHHHHH
FQERGVPAWQRDVPLLHVGETLLFVPRLGVNRAPEAGGAGAGVAGPCRRIEWRPDLLIA
HHHCCCCCHHHCCCEEEECCHHEECCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA